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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
黄嘌呤氧化酶(XOD,EC 1.17.3.2)属于需氧脱氢酶类,是活性氧的主要来源之一,也是核苷酸代谢的关键酶。该酶主要分布于哺乳动物的肝脏等组织中,当肝功能受损时,XOD大量释放入血,对肝损伤的诊断具有特异性意义。XOD催化黄嘌呤氧化生成尿酸和超氧阴离子自由基,后者与显色剂反应生成有色产物,通过测定有色产物的生成量,即可计算XOD的活性。
Xanthine oxidase (XOD, EC 1.17.3.2) is an aerobic dehydrogenase. It is a major source of reactive oxygen species (ROS) and also serves as a key enzyme in nucleotide metabolism. This enzyme is predominantly distributed in the liver and other tissues of mammals. When hepatic function is impaired, XOD is released into the bloodstream in large quantities, which holds specific diagnostic significance for liver injury. XOD catalyzes the oxidation of xanthine to uric acid and superoxide anion radicals (O₂⁻·). These radicals subsequently react with a chromogenic reagent to generate a colored product. The XOD activity can be calculated by measuring the amount of the colored product formed.