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BioReagent,适用于微生物学 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本产品为 2 倍浓度 YT肉汤培养基,在经典配方基础上将胰蛋白胨与酵母浸粉浓度提升一倍,营养更充沛。能显著提高大肠杆菌的菌体密度,是重组质粒扩增、重组蛋白诱导表达的绝佳选择。
核心优势
• 营养加倍,高效扩繁:16g/L 胰酪蛋白胨 + 10g/L 酵母浸粉,为重组大肠杆菌(如 BL21、Rosetta 等)提供充足的生长因子,轻松达到高密度发酵要求。
• 配方经典,兼容性强:5g/L 氯化钠维持适宜渗透压,完美兼容各类抗生素筛选(如 Amp、Kana 等),适合各类重组菌株培养。
• 即用型粉剂,操作便捷:按比例称量溶解即可,减少繁琐的配制误差。
培养基配方(每升):
组分 | 含量 |
胰酶水解酪蛋白(胰酪蛋白胨) | 16.0g |
酵母浸粉 | 10.0g |
氯化钠 | 5.0g |
使用说明:
称取本品 31.0 克于 1L 蒸馏水或去离子水中 (可按比例增加或减少配制量),加热煮沸至完全溶解,分装,121℃ 高压灭菌 15 分钟,冷却,备用。
注意事项:
1、pH7.0±0.2 (25℃)。
2、操作时避免与皮肤、眼睛直接接触,如不慎接触,请立即用大量清水冲洗。
3、本品仅用于实验室科研实验,不可用于临床诊断。
This product is 2× YT Broth Medium. Compared with the classic formula, the concentrations of tryptone and yeast extract are doubled for richer nutrition. It can significantly increase the cell density of Escherichia coli and is an excellent choice for recombinant plasmid amplification and recombinant protein induction and expression.
Key Advantages
• Double nutrition for efficient propagation: Contains 16 g/L tryptone + 10 g/L yeast extract, providing abundant growth factors for recombinant E. coli (e.g., BL21, Rosetta) to readily meet high-density fermentation requirements.
• Classic formula with broad compatibility: 5 g/L sodium chloride maintains suitable osmotic pressure, fully compatible with various antibiotic selections (e.g., Amp, Kana), suitable for culture of diverse recombinant strains.
• Ready-to-use powder for easy operation: Weigh and dissolve as required to reduce errors from complicated preparation.
Medium Formula (per liter):
Component | Content |
Tryptone | 16.0g |
Yeast Extract | 10.0g |
Sodium Chloride | 5.0g |
Instructions for Use:
Weigh 31.0 g of the product into 1 L of distilled or deionized water (the preparation volume can be increased or decreased proportionally), heat to boiling until completely dissolved, dispense, autoclave at 121 °C for 15 minutes, cool and set aside for use.
Precautions:
1. pH 7.0±0.2 at 25 °C.
2. Avoid direct contact with skin and eyes during handling. In case of accidental contact, rinse immediately with plenty of clean water.
3. This product is for laboratory research only and is not intended for clinical diagnosis.
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Suitable for microbiology 级别指南 →