ZNL0325 is a covalent probe based on pyrazolopyrimidine. ZNL0325 features an acrylamide side chain at the C3 position, which is capable of forming covalent bonds with multiple kinases that possess a cysteine at the αD-1 position, including BTK , EGFR , BLK , and JAK3. ZNL0325 can be used in the research of creating structurally distinct covalent kinase inhibitors.