BioReagent,适用于分析 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
锌是必需的微量元素之一，在胰岛素和卟啉代谢中也起重要作用。本试剂盒适用于检测血清、血浆、尿液、乳汁中的锌含量。
本试剂盒检测原理如下：在特定的酸碱度条件下，锌离子与5-Br-PADAP 能形成稳定的、具有特征颜色的络合物，颜色的深浅与锌离子浓度成正比。
产品组分
Z1509014 | 组件 | 物理外观 | 100T | 储存 | 单次测试使用量 |
Z1509014A | 蛋白沉淀剂 | 液体 | 10 mL | 2-8℃，避光 | 按需 |
Z1509014B | 显色剂 | 液体 | 100 μL | 2-8℃，避光 | 1 μL |
Z1509014C | 缓冲液 | 液体 | 20 mL | 2-8℃ | 200 μL |
Z1509014D | 标准品（1.54 mmol/L） | 液体 | 1 mL | 2-8℃，避光 | 按需 |
使用方法
1. 样本上清液的制备
按样本：蛋白沉淀剂为1：1 比例混匀，4℃，13780 × g 离心10 min，取上清液待测。
注意：离心后的上清液必须澄清，若有浑浊，须将浑浊上清转移至新的离心管，再次离心。
2. 标准品的配制
按照下表用双蒸水将标准品（1.54 mmol/L）稀释至0、1.54、7.7、15.4、23.1、30.8、38.5、46.2 μmol/L。
编号 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
浓度（μmol/L） | 0 | 1.54 | 7.7 | 15.4 | 23.1 | 30.8 | 38.5 | 46.2 |
标准品（μL） | 0 | 1 | 5 | 10 | 15 | 20 | 25 | 30 |
双蒸水（μL） | 1000 | 999 | 995 | 990 | 985 | 980 | 975 | 970 |
3. 显色工作液配制
按显色剂：缓冲液为1：200比例混匀，现用现配。
4. 样本测定
按照下表进行样本测定（在96孔板中反应）。
| 标准孔 | 测定孔 |
待测样本（μL） | - | 50 |
不同浓度标准品（μL） | 50 | - |
显色工作液（μL） | 200 | 200 |
混匀，静置5min，使用酶标仪测定560nm处OD值。 |
5. 结果计算
标准品拟合曲线：y = ax + b
锌含量计算公式：锌含量( μmol/L)=(ΔA-b) ÷ a × 2* × f
y：标准品OD 值-空白OD 值(标准品浓度为0 时的OD 值)；
x：标准品的浓度；
a：标曲的斜率；
b：标曲的截距；
ΔA：样本OD 值-空白OD 值(标准浓度为0 时的OD 值)；
2*：样本前处理时的稀释倍数；
f：样本加入检测体系前的稀释倍数
注意事项
1. 样本不能溶血；不能使用EDTA、柠檬酸盐等金属螯合剂作为抗凝剂。
2. 所用器皿和试剂避免锌离子污染。
3. 正式检测前，建议选择 2-3 个预期差异较大的样本进行预实验。
4. 为了您的安全和健康，请穿实验服并戴乳胶手套操作。
Zinc is one of the essential trace elements and plays an important role in insulin and porphyrin metabolism. This kit is suitable for detecting zinc levels in serum, plasma, urine, and milk.
The detection principle of this kit is as follows: under specific pH conditions, zinc ions form a stable, colorimetric complex with 5-Br-PADAP, the intensity of which is directly proportional to the concentration of zinc ions.
Kit Contents
Z1509014 | Components | Appearance | 100T | Storage | Quantity Per Test |
Z1509014A | Protein Precipitator | Liquid | 10 mL | 2-8℃. Store in the dark. | As needed |
Z1509014B | Chromogenic Agent | Liquid | 100 μL | 2-8℃. Store in the dark. | 1 μL |
Z1509014C | Buffer Solution | Liquid | 20 mL | 2-8℃ | 200μL |
Z1509014D | Standard Solution (1.54 mmol/L) | Liquid | 1 mL | 2-8℃. Store in the dark. | As needed |
Instructions for Use
1. Preparation of Sample Supernatant
Mix the sample with protein precipitation reagent in a 1:1 ratio, centrifuge at 4°C and 13,780 × g for 10 minutes, then collect the supernatant for testing.
Note: The supernatant after centrifugation must be clear. If turbidity is present, transfer the turbid supernatant to a new centrifuge tube and centrifuge again.
2. Preparation of standards
Using deionized water, dilute the standards (1.54 mmol/L) to 0, 1.54, 7.7, 15.4, 23.1, 30.8, 38.5, and 46.2 μmol/L according to the table below.
Serial Number | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
Concentration ( μmol/L) | 0 | 1.54 | 7.7 | 15.4 | 23.1 | 30.8 | 38.5 | 46.2 |
Standard solution (μL) | 0 | 1 | 5 | 10 | 15 | 20 | 25 | 30 |
Deionized water (μL) | 1000 | 999 | 995 | 990 | 985 | 980 | 975 | 970 |
3. Preparation of Chromogenic Working Solution
Mix Chromogenic Agent and Buffer Solution at a volume ratio of 1:200. Prepare immediately before use.
4. Sample Measurement
Carry out the sample measurement according to the table below (with the reaction taking place in a 96-well plate).
Reagent name | Standard well | Sample well |
Sample (μL) | - | 50 |
Standard solutions of different concentrations (μL) | 50 | - |
Chromogenic Working Solution (μL) | 200 | 200 |
Mix well, incubate for 5 min, then measure the OD value at 560 nm using a microplate reader. |
5. Result calculation
Standard curve equation: y = ax + b
Zinc Content Calculation: Zinc content (μmol/L) = (ΔA - b) / a × 2* × f
y: measured OD value minus blank OD value (OD at zero standard concentration);
x: standard concentration;
a: slope of the standard curve;
b: intercept of the standard curve;
ΔA: Sample OD value - blank OD value (OD value at standard concentration = 0);
2*: Dilution factor during sample pretreatment;
f: dilution factor applied to the sample prior to addition into the assay system.
Precautions
1. The sample must not cause hemolysis; metal chelating agents such as EDTA and citrate cannot be used as anticoagulants.
2. The used containers and reagents should be free from zinc ion contamination.
3. Before the formal test, it is recommended to select 2-3 samples with significant expected differences for a preliminary experiment.
4. For your safety and health, please wear a lab coat and latex gloves during the operation.
Z1509014 | 组件 | 物理外观 | 100T | 储存 | 单次测试使用量 |
Z1509014A | 蛋白沉淀剂 | 液体 | 10 mL | 2-8℃，避光 | 按需 |
Z1509014B | 显色剂 | 液体 | 100 μL | 2-8℃，避光 | 1 μL |
Z1509014C | 缓冲液 | 液体 | 20 mL | 2-8℃ | 200μL |
Z1509014D | 标准品（1.54 mmol/L） | 液体 | 1 mL | 2-8℃，避光 | 按需 |
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0738041 |
分析证书 | Z1509014 |
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