酵母裂解酶

CAS: 37340-57-1 货号: Z1523272 EC号: 609-409-4
有货
级别和纯度: 生物活性 ? 生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。 ActiBioPure™ ? ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。 天然 ? 天然型(Native)—— 处于天然(非重组、非变性)形态的蛋白/生物分子。当需要天然结构和活性时使用。 高性能 ? 高性能级 —— 纯度和性能特性经过优化。适用于普通等级无法满足的灵敏分析。 EnzymoPure™ ? EnzymoPure™ —— 阿拉丁的高质量酶解决方案系列。当酶纯度和明确活性决定检测或工艺性能时使用。 from Arthrobacter luteus; ≥100,000 U/g enzyme powder
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规格
库存
价格
数量
5mg
Z1523272-5mg
期货 Stock Image
¥1,399.90
25mg
Z1523272-25mg
期货 Stock Image
¥3,999.90
100mg
Z1523272-100mg
期货 Stock Image
¥9,999.90
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为什么选择此级别

ActiBioPure™,EnzymoPure™,天然,生物活性,高性能 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述


Zymolyase, produced by a submerged culture of Arthrobacter luteus, has strong lytic activity against living yeast cell walls, to produce protoplast or spheroplast of various strains of yeast cells. An essential enzyme for the lytic activity of Zymolyase is β-1,3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with β-1,3-linkages and releases specifically laminaripentaose as the main and minimum product unit. Essential enzyme: β-1,3-glucan laminaripentaohydrolase ptimum pH and Temp.: pH7.5, 35°C (for lysis of viable yeast cells) pH6.5, 45°C (for hydrolysis of yeast glucan) Stability (2°C): No loss of activity was found after storage for 1 year Specificity (Lytic Spectrum) : Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, kluyveromyces, Lipomyces, Metschnikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, Schwanniomyces, etc. Unit Definition One unit of lytic activity is defined as that amount which indicates 30% of decrease in absorbance at 800 nm(A800) of the reaction mixture under the following condition. Reaction Mixture Enzyme solution: 1 ml (0.012-0.024 mg/ml for Zymolyase) Brewer's yeast cell suspension: 3 ml (2 mg/ml) 1/15M Phosphate buffer: 5 ml (pH7.5) Distilled water: 1 ml After incubation for 2 hours at 25°C with gentle shaking, A800 of the mixture is determined. When 60% of A800 decrease, equivalent to 2 units, is observed in the reaction system, the brewer’s yeast cells are completely lysed, namely 1 unit of Zymolyase lyses 3 mg dry weight of brewer’s yeast. Preparation of 1/15 M Phosphate Buffer (1L of pH 7.5 buffer) Dissolve 19.1 g of di-sodium hydrogenphosphate 12-hydrate in distilled water to make an 800 mL solution. Dissolve 1.814 g of potassium dihydrogenphosphate in distilled water to make a 200 mL solution. Mix the two solutions.

规格

产品名称
酵母裂解酶
规格或纯度
ActiBioPure™,生物活性,高性能,EnzymoPure™,天然,from Arthrobacter luteus; ≥100,000 U/g enzyme powder
CAS编号和信息
37340-57-1
分子类型
酶
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
浓度
from Arthrobacter luteus; ≥100,000 U/g enzyme powder
储存条件
2-8°C储存
运输条件
低温运输

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

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✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

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🔬 规格说明书

该级别的完整质量属性和验收标准。

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高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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溶液计算器