计算溶液所需的质量、体积或浓度。
BioReagent,无菌过滤 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本产品由 Na₂HPO₄、NaH₂PO₄、KCl 和 MgSO₄ 组成,为 pH7.0 的缓冲液,经过过滤除菌处理。可用于配制Z buffer/X-gal 溶液,检测核系统 AH109 酵母双杂和膜系统 NMY51 酵母双杂中 LacZ 报告基因。
Z buffer 为酵母 X-gal 显色实验提供缓冲环境,使酵母菌落显色更清晰、持久。
使用说明 (以5 mL体系为例):
5 mL Z buffer中加入13.5 μL 2-巯基乙醇 (阿拉丁货号:M755744),83.5 μL X-Gal 溶液 (20 mg/mL) (阿拉丁货号:X1520260)。根据需求量可按比例扩大配制。
注意事项:
1. 注意无菌操作,避免微生物污染。
2. 本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。
3. 为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
This product is composed of Na₂HPO₄, NaH₂PO₄, KCl, and MgSO₄. It is a buffer at pH 7.0 and has been filter-sterilized. It can be used to prepare Z buffer/X-gal solution for detecting the LacZ reporter gene in the nuclear system AH109 yeast two-hybrid and the membrane system NMY51 yeast two-hybrid.
Z buffer provides a buffering environment for yeast X-gal chromogenic experiments, making yeast colonies develop clearer and more persistent color.
Instructions for Use (using a 5 mL system as an example):
Add 13.5 μL of 2-mercaptoethanol (Aladdin Catalog No.: M755744) and 83.5 μL of X-Gal solution (20 mg/mL) (Aladdin Catalog No.: X1520260) to 5 mL of Z buffer. The preparation can be scaled up proportionally according to the required amount.
Precautions:
1.Pay attention to aseptic operation to avoid microbial contamination.
2.This product is for research use only. Do not use it for pharmaceutical, clinical diagnostic or therapeutic, food, or cosmetic purposes.
3.For your safety and health, please wear a laboratory coat and disposable gloves and a mask during operation.
| pH | 7 |
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