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蛋白内切酶 Glu-C 来源于金黄色葡萄球菌 V8

蛋白内切酶 Glu-C 来源于金黄色葡萄球菌 V8

规格或纯度: ≥500 units/mg dry weight

CAS编号: 66676-43-5
MDL号: MFCD01324998
酶学委员会编号: I.U.B.: 3.4.21.19

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货号 (SKU)	包装规格	是否现货	价格	数量
P128655-1mg	1mg	期货
P128655-5mg	5mg	现货

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基本描述

别名	V8蛋白酶
规格或纯度	≥500 units/mg dry weight
英文名称	Endoproteinase Glu-C from Staphylococcus aureus V8
单位定义	One Unit causes a change of 0.001 A280 nm per minute at 37°C, pH 7.8 using casein as the substrate.
生化机理	Staphylococcus strain V8 protease specifically cleaves peptide bonds on the carboxyl side of aspartic and glutamic acid residues when used in phosphate buffer. When used in ammonium bicarbonate buffer or ammonium acetate buffer cleavage is restricted to the carboxyl side of glutamic acid residues only. The enzyme exhibits maximal activity from pH 4.0 to 7.8. If hemoglobin is used as the substrate, maximal activity is at pH 4.0. The maximal activity is at pH of 7.8 when casein is the substrate.
储存温度	2-8°C储存
运输条件	冰袋运输
产品介绍	Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay described by Drapeau (Methods Enzymol., 45, 469, 1976).Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine protease used for selective cleavage of proteins for amino acid sequence determination or peptide mapping . Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay described by Drapeau (Methods Enzymol., 45, 469, 1976). Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine protease used for selective cleavage of proteins for amino acid sequence determination or peptide mapping .

名称和标识符

酶学委员会编号	I.U.B.: 3.4.21.19
WGK Germany	3

安全和危险性（GHS）

象形图	Health Hazard Harmful
信号词	Danger
危险声明	H315: Causes skin irritation H319: Causes serious eye irritation H335: May cause respiratory irritation H317: May cause an allergic skin reaction H334: May cause allergy or asthma symptoms or breathing difficulties if inhaled
预防措施声明	P261,P305+P351+P338,P280,P342+P311
WGK Germany	3
个人防护装备	dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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