FOOD HYDROCOLLOIDS
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2025
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IF 12.4
Longwei Chen,Chi Zhang,Xi Yu,Lixiao Fu,Xiaozhi Tang,Xiao Feng
Native quinoa protein has weak processability. Fibrillation can vary the structure and foaming properties of proteins. Quinoa 2S albumin also has the potential to be an interface active substance. In this study, quinoa protein amyloid-like fibrils (QPF) and 2S albumin were blended to stabilize foams for the first time, and their interfacial behavior, foaming properties and the microstructure of foams were analyzed. The absolute value of zeta potential and surface tension of quinoa protein isolates (QPI) decreased after fibrillation, which improved the foam capacity and stability significantly. When QPF were further blended with 2S albumin at a ratio of 1:1, foam capacity and foam stability reached the highest values of 183.33% and 86.38%, respectively. The adsorption of QPF at the air-water interface along with 2S albumin’s filling in the interfacial voids may strengthen the protein interfacial network and their foaming properties. Meanwhile, our study revealed that both the viscosity of the liquid phase as well as the interfacial properties of the protein dispersion influenced the foam capacity and foam stability of QPF/2S albumin. Furthermore, foams generated from QPF/2S albumin were applied to prepare coffee latte art and chiffon cakes. The cakes using foams generated from QPF/2S albumin to fully replace egg white exhibited a fluffy, porous, and stable structure. To conclude, the fibrillated quinoa protein and 2S albumin complex showed the potential to be an innovative plant-based ingredient to generate foams and applied in food processing industry, which will contribute to the food sustainability.
研究领域:
食品科学
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生物化学
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产品关联
DOI
高影响因子
BIOORGANIC CHEMISTRY
·
2021
·
IF 5.275
Lian Xu,Li-Chao Wang,Bing-Mei Su,Xin-Qi Xu,Juan Lin
(2 S , 3 R )-4-methylsulfonylphenylserine [(2 S , 3 R )-MPS], a key chiral precursor for antibiotics florfenicol and thiamphenicol, could be asymmetrically synthesized by l -threonine transaldolase (LTTA) coupled with an acetaldehyde elimination system. The low efficiency of acetaldehyde elimination system blocked further accumulation of (2 S , 3 R )-MPS. To address this issue, strengthening acetaldehyde elimination system and enzyme self-assembly strategy were combined to accelerate biosynthesis of (2 S , 3 R )-MPS. The new multi-enzyme cascade with intensified acetaldehyde elimination system BL21 (PsLTTAD2/ScADH/BtGDH) could produce (2 S , 3 R )-MPS with a titer of 157.6 mM, 1.7-folds than that produced by the original system BL21 (PsLTTAD2/ApADH/CbFDH). Moreover, self-assembly of PsLTTAD2 and ScADH by respective fusion of SpyTag and SpyCatcher were carried out to develop a self-assembled multi-enzyme cascade BL21 (ST-PsLTTAD2/SC-ScADH/BtGDH). As a result, the yield of (2 S , 3 R )-MPS was up to 248.1 mM with 95% de . As far as we knew, that represented the highest yield of (2 S , 3 R )-MPS by enzymatic synthesis, and therefore was a promising and green route for industrial production of this valuable compound.
研究领域:
生物催化
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酶工程
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生物技术
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化学生物学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2020
·Vol. 21
·期 15
·5347
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IF 4.556
Xiao-Ran Jing,Huan Liu,Yao Nie,Yan Xu
Fe(II)/2-ketoglutarate-dependent dioxygenase (Fe(II)/2-KG DO)-mediated hydroxylation is a critical type of C–H bond functionalization for synthesizing hydroxy amino acids used as pharmaceutical raw materials and precursors. However, DO activity requires 2-ketoglutarate (2-KG), lack of which reduces the efficiency of Fe(II)/2-KG DO-mediated hydroxylation. Here, we conducted multi-enzymatic syntheses of hydroxy amino acids. Using (2s,3r,4s)-4-hydroxyisoleucine (4-HIL) as a model product, we coupled regio- and stereo-selective hydroxylation ofl-Ile by the dioxygenase IDO with 2-KG generation from readily availablel-Glu byl-glutamate oxidase (LGOX) and catalase (CAT). In the one-pot system, H2O2significantly inhibited IDO activity and elevated Fe2+concentrations of severely repressed LGOX. A sequential cascade reaction was preferable to a single-step process as CAT in the former system hydrolyzed H2O2. We obtained 465 mM 4-HIL at 93% yield in the two-step system. Moreover, this process facilitated C–H hydroxylation of several hydrophobic aliphatic amino acids to produce hydroxy amino acids, and C–H sulfoxidation of sulfur-containingl-amino acids to yieldl-amino acid sulfoxides. Thus, we constructed an efficient cascade reaction to produce 4-HIL by providing prerequisite 2-KG from cheap and plentifull-Glu and developed a strategy for creating enzymatic systems catalyzing 2-KG-dependent reactions in sustainable bioprocesses that synthesize other functional compounds.Keywords:Fe(II)/2-ketoglutarate-dependent dioxygenase;2-ketoglutarate generation;regio- and stereo-selective synthesis;hydroxy amino acids;sequential cascade reaction
研究领域:
生物化学
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生物催化
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合成生物学
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酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Ao Zhou,Dan Zhang,Zhihong Liu,Zhiyong Liu
With the retirement of rechargeable lithium-ion batteries (LIBs) entering peak period, the recycling of LiCoO 2 has become a hot topic. The spent LiCoO 2 batteries are rich in carcinogenic metal Co and high-value metal Li, so they are needed to be recovered in time before causing serious environmental pollution and resource waste. Thus, a clean and efficient process is proposed for the comprehensive recovery of Li, Co, and Mn from the spent LiCoO 2 cathode material. It is an integrated pyro-hydrometallurgical process, mainly consisting of sulfation roasting with NH 4 HSO 4 and selective sulfurizing leaching with (NH 4 ) 2 S solution. Using low melting point ammonium salt as roasting auxiliary, 99.7 %, 99.1 %, and 99.8 % of Li, Co, and Mn in the spent LiCoO 2 powder can be converted into sulfate when roasting under 400 ℃ for 75 min. Then 99.5 % of Li in the sulfation roasting product can be selectively leached with an aqueous (NH 4 ) 2 S solution, which is also a precipitator for Co, Mn, and impurity Al. In addition, 96.4 % of Co and 96.0 % of Mn in the sulfurized precipitate can be separated with impurity elements Al and C via oxidation roasting–water leaching process. Compared to traditional recycling, this method can achieve a higher Li leaching rate at a low roasting temperature, and the recovery of Co and Mn can be realized without adding any acid/base reagents or impurity cations. More importantly, the circulation of NH 3 /NH 4 + and S element in the recycling system has been taken into account, making it an environmental-friendly and economical process. In this study, the sulfation reduction roasting and selective sulfurizing leaching mechanisms were both revealed in details, and the behavior of the valuable elements was also elucidated.
研究领域:
环境科学
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冶金工程
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
SOLAR ENERGY
·
2019
·
IF 4.674
Yuqian Ai,Weiqing Liu,Chunhui Shou,Jin Yan,Nan Li,Zhenhai Yang,Wei Song,Baojie Yan,Jiang Sheng,Jichun Ye
Tin oxide (SnO 2 ) is widely adopted as an electron transport layer (ETL) in perovskite solar cells (PSCs). However, the oxygen vacancies of the SnO 2 not only are the trap states of the nonradiative recombination of photogenerated carriers, but also build the potential barrier of carrier transport. To solve this issue, ammonium sulfide [(NH 4 ) 2 S] is introduced to the SnO 2 precursor for passivating the surface defects by terminating the Sn dangling bonds (S–Sn bonds). After reducing the surface traps, the electron mobility and conductivity of SnO 2 film are enhanced significantly while the carrier recombination is decreased. Additionally, the energy level of S-SnO 2 is also slightly modified. Therefore, this sulfide-passivated mothed remarkably improves the electron collection efficiency of the ETL. Furthermore, the linkage of Sn–S–Pb anchors the perovskite crystals at the perovskite/SnO 2 interface, which increases the electron extraction efficiency and the stability of PSC. Based on this S-SnO 2 ETL, the power conversion efficiency of the PSC is greatly promoted from 18.67% to 20.03%, compared with the reference one. In this study, it is proven that the surface defect passivation of SnO 2 is an efficient and simple method to improve the photovoltaic performance, as a promising ETL for high-efficiency device.
研究领域:
材料科学
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能源科学
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光伏技术
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2022
·
IF 9.221
Suqin Xiong,Shuguang Yan,Mingxia Sun,Hongjie Song,Lichun Zhang,Jiaxi Hu,Yi Lv
Vacancy regulation is considered to be an effective means to improve the properties of materials, which has been applied in the fields of catalysis, electricity and optics. Herein, different contents of nitrogen vacancy in graphitic carbon nitride (g-C 3 N 4 ) could be modulated via changing temperature of secondary calcination . Results showed the introduction of nitrogen vacancy significantly promoted the cataluminescence (CTL) intensity towards ammonium sulfide ((NH 4 ) 2 S) and the CTL performance increased regularly with the increaseing vacancy content. The positive effect of nitrogen vacancy on CTL intensity of (NH 4 ) 2 S was unveiled in-depth by the experiment and density functional theory (DFT). Moreover, a cutting-edge CN-600-based sensor was established for the highly sensitive detection of (NH 4 ) 2 S. The sensor possessed excellent selectivity and stability, and a low detection limit (0.16 μg mL −1 ). Noteworthily, this study presents novel mentality for designing materials with improved catalytic activity .
研究领域:
材料科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
BIOORGANIC CHEMISTRY
·
2025
·
IF 4.7
Xinqi Xu,Xialian Wang,Lian Xu,Bingmei Su,Juan Lin,Qiaobin Chen
Fe(II)/α-ketoglutarate (α-KG)-dependent isoleucine dioxygenase (IDO) enables selective C-H hydroxylation to produce the antidiabetic agent (2 S , 3 R , 4 S )-4-hydroxyisoleucine ((2 S , 3 R , 4 S )-4-HIL), but its industrial application is limited by insufficient activity and stability and the high cost of α-KG. Here, the IDO from Bacillus thuringiensis was engineered using multiple strategies to generate a mutant MU3 (V146I/S105E/N104P) with increased substrate loading. The specific activity exhibited 15-fold higher (152.0 U/mg) with 15-fold improved catalytic efficiency ( k cat / K m ) for L-isoleucine hydroxylation. The thermostability was enhanced with a 10-fold decrease in thermal inactivation rate at 30 °C. Molecular dynamics simulation revealed that the communication alteration between the loops around the active site and the strengthened substrate binding contributed to the improvement of MU3. The MU3 mediated L-isoleucine hydroxylation was coupled with an optimized whole-cell module integrating L-glutamate oxidase and catalase for α-KG generation from low-cost L-glutamate sodium. By the stepwise whole-cell biocatalytic cascade system, both 500 mM and 750 mM of L-isoleucine could be completely converted into (2 S , 3 R , 4 S )-4-HIL under scaled-up condition. The whole space-time-yield reached 196 g/L/d as the highest record up to date and the cost was decreased compared to the α-KG-supplemented approach. This work provides an efficient, cost-effective and scalable route for (2 S , 3 R , 4 S )-4-HIL synthesis.
研究领域:
酶工程
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生物催化
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合成生物学
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工业生物技术
·
代谢工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2026
·
IF 18.1
Tang Chao,Shen Yuemeng,Qu Jingyao,Yu Lu,Liu Honglei,Liu Huaiwei,Xun Luying,Xia Yongzhen
Iron-sulfur clusters are versatile protein cofactors involved in diverse biological processes, but their role in hydrogen sulfide/hydrosulfide (H 2 S/HS - ) sensing remains largely unexplored. Here, we report that the Bacillus licheniformis sensor kinase NreB contains an unusual [2Fe-2S] cluster within its PAS domain. A 1.52-Å crystal structure reveals a distinct coordination geometry where three conserved cysteine residues and a non-cysteinyl sulfur ligand stabilize the cluster. Biochemical and native mass spectrometry support assignment of the predominant ligand state as -SH and show enrichment of -SSH/SSOH-like state upon sulfide exposure in the presence of O 2 , correlating with increased NreB kinase activity. Electron paramagnetic resonance spectroscopy shows that the cluster retains its oxidized [2Fe-2S] 2+ state during sulfide-sensing. Molecular dynamics simulations further reveal transient solvent and HS - accessibility to the buried cluster, providing a physical basis for ligand entry. Here, we show that bacteria sense sulfide via a three-cysteine-coordinated Fe-S cluster with a labile sulfur ligand.
✓ 已验证
DOI
PubMed
高影响因子
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