INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Wanqiu Huang,Mengdi Hao,Liangqiang Chen,Hao Shen,Shaoning Yu,Ying Han,Fan Yang
Rapid quantitative detection of β-amylase is important for food quality, production efficiency, and food safety. However, current detection methods are time-consuming with low sensitivity. Fast, sensitive, and simple detection of β-amylase remains a challenge in practice. In the present work, we constructed a colorimetric β-amylase assay based on cross-linked nanozyme. The assay utilized the interaction between β-amylase and its specific substrate, p -nitrophenyl-β-maltopentaoside (PNPG3), which cross-links with the FeCo-BDC-NH 2 metal-organic framework nanozyme. The detection mechanism is based on β-amylase hydrolyzing the crosslinking agent PNPG3 on the surface of FeCo-BDC-NH 2 , leading to enhanced peroxidase-mimicking biological activity of the nanozyme in free state. The detection limit was 2.77 U L −1 , with a broad linear range (5–160 U L −1 ) and remarkable specificity. In addition, the assay was used in fermentation samples to validate the analytical performance. This assay offers an accessible and efficient method of determining β-amylase activity in the actual production.
研究领域:
食品科学
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生物化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Yingxin Wu,Kainan Hong,Juzhen Yi,Juncheng Shen,Liqun Yang
Surface-enhanced Raman spectroscopy (SERS) shows unique advantages for the study of protein structure in water environment, because it can provide plenty of structural information of proteins in addition to very weak Raman scattering signal of water. To achieve the Raman-enhanced effect of proteins, the challenge is to prompt protein molecules to approach the surface of noble metal (such as Ag and Au). Based on the enzyme–substrate specificity, the Ag nanoparticles-encapsulated amylopectin (Amyp/AgNPs) colloid was prepared as an aqueous SERS substrate to obtain the Raman-enhanced effect of amylase. Dynamic light scattering analysis showed that the hydrodynamic diameter of the Amyp/AgNPs colloid was in the range of 40–700 nm. UV–vis spectroscopy and energy dispersive spectroscopy confirmed the formation of Ag nanoparticles in the Amyp/AgNPs colloid. The Ag nanoparticles in the Amyp/AgNPs colloid were observed as globular homogeneous particles in the sizes ranging from 10 to 20 nm using transmission electron microscopy (TEM). The crystalline Ag nanoparticles were further observed in the high-resolution TEM image. The obviously enhanced Raman bands assigned to the amino acid residues and the secondary structures of α -amylase and β -amylase appeared in their SERS spectra, which were further analyzed following the principal component analysis (PCA) process. The Raman-enhanced mechanism is considered as that the specificity of two amylases with amylopectin in the Amyp/AgNPs colloid may prompt them to approach the surface of Ag nanoparticles and then produce the significant SERS effect. The SERS strategy based on enzyme–substrate specificity is thus anticipated to apply for structural analysis of enzyme.
研究领域:
生物化学
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分析化学
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纳米技术
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DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Hao Shen,Mengdi Hao,Shaoning Yu
β-Amylase (BMY) is a linchpin in food production and the pharmaceutical industry because the enzyme efficiently controls the ratio of diverse saccharides in fermentation and the manufacture of high-quality maltose. However, existing BMY detection tactics suffer from inadequate selectivity/sensitivity and cumbersome operation and do not meet the needs of precise quantification. Consequently, there is an urgent need to develop an ultrasensitive sensing platform to achieve precise BMY analysis with a low detection limit and simpler workflow. In this work, we establish an encapsulated-enzyme-based BMY biosensing platform in which α-glucosidase is embedded in magnetic layered double hydroxide using a self-sacrificing template. The encapsulated enzyme has increased activity, robustness, and recyclability and was utilized for BMY detection via a cascade chromatic process. We found a detection limit for the quantification of BMY activity of 2.67 U/L with a broad range (5–400 U/L), fast response speed (10 min), and satisfactory specificity. We applied the biosensing platform to liquor starters to verify the capability of the assay in complicated fermentation samples. The proposed platform holds great promise as an efficient and simple method for enzymatic bioactivity monitoring in food manufacturing, biopharmaceutical processing, and clinical laboratory tests.
研究领域:
食品科学
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生物技术
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分析化学
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产品关联
DOI
PubMed
高影响因子
POLYMERS FOR ADVANCED TECHNOLOGIES
·
2020
·Vol. 31
·期 12
·2967-2979
·
IF 2.578
Chao Ma,Jide Wang,Liqin Cao
The current study reports the utilization of MIL-100(Fe) as Pickering emulsifiers to stabilize CO 2 -in-water high internal phase emulsions, thereby synthesizing interconnected macroporous hybrid monoliths with adjustable pore sizes. During such a green synthesis procedure, the influences of Fe-MOF type and content, carbon dioxide density, and the amount of crosslinker have been explored respectively. The as-synthesized MOF-containing poly(HIPE)s were characterized by FT-IR, XRD, SEM, CLSM, and TGA. The morphology of the monoliths was mainly observed by EDS-mapping and SEM, which showed that Fe-MOFs were uniformly distributed into the monolithic matrix and the void sizes and interconnected pore sizes were 20 to 150 μm and 5 to 50 μm, respectively. Furthermore, the MIL-100(Fe)-containing monoliths exhibited an excellent elasticity and can quickly return to their original state when reaching up to 90% compressive strain without failing. To evaluate the adsorptive properties of the composite monoliths, the immobilization of β-amylase on the obtained monoliths was studied. The results of immobilized enzymes showed that the highest protein adsorption rate can reach 55% and the immobilization efficiency can reach 89%. Since our synthetic route is clean and controllable, biocompatible monoliths with good elasticity and toughness were prepared, which presents a viable strategy to produce hybrid monoliths for bio-medical applications.
研究领域:
材料科学
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生物医学工程
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酶工程
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2020
·
IF 5.162
Xiong Lin,Siwei Sun,Bailong Wang,Baodong Zheng,Zebin Guo
Lotus seed starch (LS) was enzymatically hydrolyzed by pullulanase, β-amylase and α-amylase to obtain three types of starch nanoparticles (SNPs): P-SNPs, β-SNPs and α-SNPs, respectively. The structure and physicochemical properties of lotus seed starch nanoparticles (LS-SNPs) were systematically studied by Laser particle size analysis, Scanning electron microscope (SEM), X-ray diffraction (XRD), Raman spectrometry, Nuclear magnetic resonance (NMR) and Gel permeation chromatography (GPC). The results showed that compared to LS (10,466.7 ± 230.9 nm), the D [3,2] of P-SNPs were (334.7 ± 16.2), which indicated that LS was decomposed into LS-SNPs with low degree of polymerization. P-SNPs showed the highest efficiency, with 81.74% of samples reaching the nanoscale. The particle size order was LS > β-SNPs (α-SNPs) > P-SNPs. XRD results showed P-SNPs had the highest crystallinity 65.07%. During preparation of LS-SNPs, crystal region was destroyed, and double helix structure became stronger, but no new functional groups were introduced. P-SNPs had the smallest amorphous area and also showed a minimal molecular weight and the broadest molecular weight distribution. P-SNPs had the smallest particle size and the highest crystallinity, so P-SNPs were the best of all LS-SNPs.
研究领域:
食品科学
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材料科学
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化学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Hao Huang,Fatima-ezzahra Ettoumi,Li Li,Yanqun Xu,Zisheng Luo
Economic value of the global potato harvest is impacted by sprouting during storage. We examined how sprouting might be reduced or eliminated using citral , a naturally occurring component in citrus fruit peel. The current study integrated both loading and sustained release of citral using emulsification-based interfacial synthesis of hollow MIL-88A. The structural properties and compositions of MIL-88A and hollow MIL-88A were confirmed using SEM, EDS, and XRD. BET analysis showed a surface area of 30.36 m 2 g −1 , pore volume of 0.21 cm 3 g −1 , and an average pore radius of 13.56 nm for hollow MIL-88A. Citral was successfully loaded into 10 g of MIL-88A and hollow MIL-88A, with a total citral load of 0.21 cm 3 and 1.82 cm 3 , respectively. The citral-loaded hollow MIL-88A induced a sustained release of citral, which effectively inhibited the sprouting, leading to higher starch content by 41%, lower weight losses, reducing sugar content, α-Amylase, β-amylase, and starch phosphorylase activities by 75%, 55%, 34%, 31%, and 43%, respectively. The citral-loaded hollow MIL-88A might inhibit sprouting by suppressing gibberellin and indole-3-acetic acid while maintaining abscisic acid.
研究领域:
农业科学
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食品科学
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材料科学
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植物生理学
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DOI
高影响因子
Foods
·
2024
·Vol. 13
·期 17
·2822
·
IF 4.7
Chun Zhan,Ruqi Jia,Shuzhen Yang,Meihong Zhang,Litao Peng
Chestnuts become sweetened with better tastes for fried products after cold storage, but the possible mechanism is not clear. The dynamics of sugar components and related physiological responses, as well as the possible molecular mechanism in chestnuts during cold storage, were investigated. Sucrose accumulation and starch degradation contributed to taste improvement. Sucrose content reached the peak after two months of cold storage, along with the accumulation of reducing sugars of maltose, fructose and glucose to a much lesser extent. Meanwhile, alpha-amylase and beta-amylase maintained high levels, and the activities of acid invertase and sucrose synthase increased. Transcriptome data demonstrated that differentially expressed genes (DEGs) were significantly enriched in the process of starch and sucrose metabolism pathway, revealing the conversion promotion of starch to sucrose. Furthermore, DEGs involved in multiple phytohormone biosynthesis and signal transduction, as well as the transcription regulators, indicated that sucrose accumulation might be interconnected with the dormancy release of chestnuts, with over 90% germinated after two months of cold storage. Altogether, the results indicated that cold storage improved the taste of chestnuts mainly due to sucrose accumulation induced by DEGs of starch and sucrose metabolism pathway in this period, and the sweetening process was interconnected with dormancy release.
研究领域:
植物生理学
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分子生物学
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食品科学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2024
·Vol. 89
·期 2
·941-953
·
IF 3.2
Guoxing Chen,Ruixuan Zhao,Yixuan Zhang,Qiannan Liu,Zhiqian Guo,Guohui Zhang,Tao Wu,Wei Liu,Honghai Hu
The interest in incorporating potatoes into wheat dough is increasing. However, potatoes exhibit significant viscosity during thermal processing, affecting product processing and quality. This study aims to find an effective method to reduce the viscosity of mashed potatoes. We aimed to compare the effects of different enzymes (α-amylase, β-amylase, and flavourzyme) and concentrations (0.01%, 0.05%, and 0.1%) on the micromorphology and rheological properties of mashed potatoes and potato–wheat dough. The impact of flavourzyme was the most significant ( p <0.05). When enzyme concentration increased, viscosity decreased, and the degree of structural damage, indicated by increased porosity. Notably, the addition of flavourzyme can increase the content of sweet and umami free amino acids, improving the flavor of mashed potatoes. The scanning electron microscopy and confocal laser scanning microscopy images of potato–wheat dough revealed that enzyme-hydrolyzed mashed potatoes had improved homogeneity, reestablished the dough continuity, and strengthened the three-dimensional structure comprising proteins and starch. Notably, flavourzyme demonstrated the most significant effect on enhancing the protein–starch network structure. This was attributed to the exposure of functional groups resulting from protein hydrolysis, facilitating interaction with starch molecules. Our findings indicate that the addition of 0.1% flavourzyme (500 LAPU/g, pH 5.5, 55 ± 2°C, 30 min treated) was the most effective in reducing viscosity and reconstructing the gluten network. Enzymatic hydrolysis plays a vital role in the production of high-quality potato products, with particular importance in the baking industry, where flavourzyme exhibits significant potential. Practical Application Enzymatic hydrolysis plays a vital role in the production of high-quality potato products, with particular importance in the baking industry, where flavourzyme exhibits significant potential.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Juan Tan,Shuaishuai Ding,Ling He,Xiu-wu Bian,Gan Tian
Alkaline phosphatase (ALP), a pivotal hydrolase in human tissues, serves as a key biomarker for disease diagnostics. Here, we report a hollow AuAg@CeO 2 plasmonic nanozyme engineered for ALP detection through triple synergistic catalysis. The hierarchically designed hollow architecture enhances near-infrared (NIR) photon capture via multiscale light scattering, while the AuAg-CeO 2 heterojunction enables directional charge transfer. ALP-mediated ascorbic acid generation triggers a cascade mechanism: (1) plasmonic hot electrons regenerate CeO 2 oxygen vacancies to optimize H 2 O 2 adsorption; (2) photothermal activation facilitates H 2 O 2 dissociation; and (3) localized surface plasmon resonance (LSPR) amplifies interfacial electron kinetics. This synergy boosts its peroxidase-like activity, achieving nanomolar ALP sensitivity—a 100-fold improvement over commercial kits within 10-min. Integrated smartphone-based colorimetric dual-mode analysis enables cross-validated ALP quantification with <10 % matrix interference, validated by human samples with 94.8–105.4 % recovery. By deciphering structure-LSPR-activity correlations, this work pioneers adaptive nanozyme design and intelligent diagnostic platforms for precision medicine, bridging critical gaps in point-of-care testing and early disease surveillance.
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产品关联
DOI
PubMed
高影响因子
Bioresources and Bioprocessing
·
2015
·Vol. 2
·期 1
·1-8
·
IF 0
Yang Xin,Zhu Liying,Jiang Ling,Xu Qing,Xu Xian,Huang He
Background Trehalose has many advantages due to its inertness and the ability to stabilize biomolecules. Trehalose synthase can catalyze intramolecular rearrangement of the inexpensive maltose into trehalose in a single step, which represents a simple, fast, and low-cost method for the future industrial production of trehalose. Results In this work, an intelligent visualization method for producing trehalose via trehalose synthase was for the first time established and optimized by corresponding enzymatic hydrolysis with kudzu root starch as the initial raw material. For the first step, kudzu root starch was liquefied by α-amylase at a certain dextrose equivalent value of 19–21, and β-amylase and pullulanase to saccharify for a certain time, a four-factor nine-level experimental design with nine experiments was performed according to the uniform design table U
* 9
(9 4 ) to optimize the yield of maltose. Then, optimization of trehalose conversion ratio from kudzu root starch hydrolysate was carried out with a four-factor 12-level experimental design to forecast the optimal process conditions. By comparing verification tests and predicted results, the optimized operating conditions were pH 7.1, 22 °C, 32.5 % (14,000 U/ml) loading amounts of TreS enzyme and after 24 h, along with a 70.6 % trehalose conversion rate. Conclusions The intelligent visualization method has succeeded in exploiting the conversion process of trehalose from kudzu root starch hydrolysate, which contributes significant benefits to the further commercial production of trehalose.
研究领域:
生物化学
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食品科学
·
工业微生物学
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产品关联
DOI
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Xiangpeng Han,Mengyu Zhang,Jiayi Peng,Jinsong Wu,Qingping Zhong
Vibrio parahaemolyticus and its biofilm threaten food safety and human health. Bacteriocins are known to inhibit various foodborne bacteria and their biofilms; however, few bacteriocins with antibiofilm activity against V. parahaemolyticus have been reported. Herein, a novel plantaricin Z057 from Lactiplantibacillus plantarum Z057 was extracted with ethyl acetate, purified by Sephadex LH-20 gel chromatography and reversed-phase high-performance liquid chromatography, and identified by nano-liquid chromatography coupled with tandem mass spectrometry. Its molecular mass and amino acid composition were 1272.61 Da and A-G-G-D-G-G-H-G-G-S-I-I-L-Y, respectively. Plantaricin Z057 presented a broad antibacterial spectrum against Gram-positive and Gram-negative pathogens. Moreover, it also exhibited excellent heat tolerance, maintained antibacterial activity at pH 2.0–4.0, and was susceptible to common proteases (pepsin, trypsin, papain, proteinase K). In addition, scanning electron microscopy revealed that plantaricin Z057 destroyed membrane integrity and caused cell deformation and cytoplasmic leakage. The crystal violet staining and XTT reduction assays revealed that plantaricin Z057 markedly decreased biofilm biomass and metabolic activity of V. parahemolyticus . Fluorescence microscopy showed that plantaricin Z057 efficiently inactivated sessile cells, and damaged integrated biofilm structure by reducing the extracellular polymer content. In summary, plantaricin Z057 exhibited significant potential for controlling V. parahaemolyticus and could be used in food production as a biological preservative.
研究领域:
微生物学
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食品科学
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生物技术
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分子生物学
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Yao Lin,Yuyang Li,Hongqi Chang,Simin Ye,Yi Ye,Lin Yang,Linchuan Liao,Hao Dai,Zeliang Wei,Yurong Deng,Jinyi Zhang,Chengbin Zheng
Excessive consumption of Δ9-tetrahydrocannabinol (THC) severely endangers human health and has raised public safety concerns. However, its quantification by readily rapid tools with simplicity and low cost is still challenging. Herein, we found that a G-rich THC aptamer (THC1.2) can tightly bind to thioflavin T (ThT) with strong fluorescence, which would be specifically quenched in the presence of THC. Based on that, a label-free ratiometric fluorescent sensor for the sensing of THC and its metabolite (THC-COOH) based on THC1.2/ThT as a color emitter and red CdTe quantum dots as reference fluorescence was constructed. Notably, a transition of the fluorescent color of the ratiometric probe from green to red can be instantly observed upon the increased concentration of THC and THC-COOH. Furthermore, a portable smartphone-based fluorescence device integrated with a self-programmed Python program was fabricated and used to accomplish on-site monitoring of THC and THC-COOH within 5 min. Under optimized conditions, this ratiometric fluorescent sensor allowed for an instant response toward THC and its metabolite with considerable limits of detection of 97 and 254 nM, respectively. The established sensor has been successfully applied to urine and saliva samples and exhibited satisfactory recoveries (88–116%). This ratiometric fluorescent sensor can be used for the simultaneous detection of THC and THC-COOH with the advantages of rapidness, low cost, ease of operation, and portability, providing a promising strategy for on-site detection and facilitating law enforcement regulation and roadside control of THC.
研究领域:
分析化学
·
生物医学工程
·
药物检测
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）