CHEMISTRY OF NATURAL COMPOUNDS
·
2024
·
IF 0.8
Tan Jin-Ni,Lai Chun-Hua,Wei Wei,Tan Qin-Gang
Six lupane triterpenoids ( 1 – 6 ), including an undescribed one, 3,23-di- O -isopropylidene-lup-20(29)-ene ( 1 ), along with two oleane triterpenoids ( 7 , 8 ), were isolated from the Zhuang ethnic medicinal plant Glochidion eriocarpum. Their structures were identified based on comprehensive NMR, MS, and physiochemical data analysis. Compounds 2 , 7 , and 8 significantly inhibited the production of nitride oxide (NO) of the LPS-induced RAW 264.7 cells, which may be attributed to the anti-inflammatory activity of this plant.
研究领域:
药用植物学
·
药理学
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天然产物化学
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DOI
高影响因子
ANALYST
·
2019
·Vol. 144
·期 23
·6981-6988
·
IF 4.019
Xiaoyan Liao,Fangli Hu,Zilin Chen
Triterpenoids, as an important family of plant secondary metabolites, have important biological activities associated with health and disease prevention. In this work, we proposed a HPLC-MS method for profiling multiple groups of triterpenoid acids and triterpenoid esters differing only in one position of the hydroxyl or methyl group in O. fragrans fruits. A total of thirty-one compounds were identified, and twenty-seven components were discovered in O. fragrans fruits for the first time. The HPLC-MS profiling method was applied in the analysis of the triterpenoids of O. fragrans flowers, and the time courses of triterpenoids of O. fragrans fruits.
研究领域:
分析化学
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植物化学
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植物代谢组学
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产品关联
DOI
PubMed
高影响因子
BMC Complementary Medicine and Therapies
·
2025
·Vol. 25
·期 1
·1-13
·
IF 3.4
Yang Tian,Yan YiFan,Xu YiYing,Chen YiLong,Zhi DaLong,Zhang Yan,Xiao ChaoNi
Background Loquat (Eriobotrya japonica Lindl.) fruit has been used as a heath food and folk medicine for preventing and treating the respiratory tract inflammation diseases, however, the active compounds responsible for anti-inflammation effects remain unclear.Methods Cysteinyl leukotriene receptor-1 (CysLTR1) has become an important target protein for developing therapeutics to manage the inflammation-associated diseases. Herein, a rapid approach was established by immobilizing CysLTR1 onto microspheres for the column packing, and it was applied in screening the active compounds from Loquat fruit.Results The screened compounds targeting CysLTR1 were identified as corosolic acid, ursolic acid and oleanolic acid in combination with Mass Spectrum. The high-dose combination of the three ones exhibited the stronger anti-inflammatory activity than triterpene acid alone and the Loquat extract, which can significantly decrease the counts of inflammatory cells and inhibit the production of pro-inflammatory mediators as well as increase the secretion of anti-inflammatory mediator in the LPS-inflamed mice. It was proved that three triterpene acids exhibited anti-inflammatory activities by the attenuation of CysLTR1 expression.Conclusion sOur results indicated that CysLTR1-immobilized column was a rapid and effective tool to seek out the target-specific components from herbal extract, and the screened triterpene acids could serve as alternative anti-inflammatory agents to Loquat fruit.
研究领域:
药理学
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植物化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Weiwei Zheng,Suqing Lan,Weixi Zhang,Bingqian Nie,Kai Zhu,Xingqian Ye,Zhiqiang Hou,Shiguo Chen
The polysaccharides and triterpenes are important functional components of Ganoderma lucidum , but traditional preparation process of G. lucidum functional components can only realize the preparation of single functional component, which has poor targeting and low efficiency. In this study, the existence state of the functional components of G. lucidum was revealed. Then, the single step extraction process for functional components was established, and the precise structure evaluation of polysaccharide and triterpenes was conducted based on the process. The results showed that preparation time required for this strategy is only one-sixth of the traditional one, and 50 % of raw materials can be saved. Structural analysis of the functional components revealed that triterpenes were mainly Ganoderic acid and Lucidenic acid, and the polysaccharide structure was mainly 1,3-glucan and 1,3,6-glucan. The establishment of single step extraction strategy and the evaluation of the fine structure of functional components improved the efficiency of preparation and result determination, and provided an important basis for the development and utilization of green and low-carbon G. lucidum and even edible fungi resources and human nutritional dietary improvement strategies.
研究领域:
植物化学
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食品科学
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药用真菌学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Yaping Duan,Congmin Liu,Xiaowen Dou,Liu Wu,Hao Liu,Linan Shan,Shihai Yang,Jiaoyang Luo,Meihua Yang
Triptolide is a characteristic chemical component and quality marker of Tripterygium wilfordii Hook. F., a widely used traditional Chinese medicine with various pharmacological activities. Triptolide-14-succinate is a derivative of triptolide that can be combined with bovine serum albumin and ovalbumin to form an immunogen and coating antigen. Monoclonal antibody specific to triptolide-14-succinate conjugated with ovalbumin was produced and named mAb 2D10 1H8. An indirect competitive enzyme-linked immunosorbent assay (icELISA) was established by using mAb 2D10 1H8. The 50% inhibition concentration and working range of triptolide for the icELISA were 0.28 and 0.09–0.87 μg mL −1 , (based on 20–80% binding inhibition of mAb 2D10 1H8 to triptolide, R 2 = 0.9997, y = -0.571Logx + 0.246), respectively. The icELISA displayed crossreactivity values of 48.6, 20.5, 3.27, and < 1.00% for triptonide, triptophenolide, tripterifordin, and other analogs of triptolide, respectively. The average recovery of triptolide from the Tripterygium wilfordii samples as determined by icELISA ranged from 90.05 to 106.6%. Triptolide was detected in 38 batches of Tripterygium wilfordii samples by icELISA, which was validated by ultra-fast liquid chromatography combined with electrospray ionization tandem-mass spectrometry. In conclusion, the icELISA method established in the present study is applicable for rapid authentication of Tripterygium wilfordii and related products.
研究领域:
中药学
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免疫学
·
分析化学
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2021
·
IF 5.645
Mingming Zhou,Siew Young Quek,Xulan Shang,Shengzuo Fang
This research investigated the variations of triterpenoid contents in Cyclocarya paliurus leaves and their underlying anticancer activity and mechanism. Leaf samples were collected from twelve geographical locations in China for the experiments. Significant variation in the triterpenoid content and growth inhibition on HeLa cells were observed among the C. paliurus populations. Antiproliferation effects of the leaf samples were significantly affected by the pentacyclic triterpenoids contents except for oleanolic acid. Among the pentacyclic triterpenoids, hederagenin exhibited the best anticancer activity with IC 50 of 17.42 μg/mL. Based on the results, pentacyclic triterpenoids may induce HeLa cell apoptosis via mitochondrial pathway. RNA sequencing indicated that the ACTB , ATF4 , TUBA1B and TUBA1C genes might be the key ones involved in the apoptosis induction by cyclocaric acid B and hederagenin. These results provided useful information for application of C. paliurus in functional beverage and pharmaceutical industries.
研究领域:
药理学
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植物科学
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分子生物学
·
癌症研究
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产品关联
DOI
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2017
·
IF 2.981
Zi-wei Li,Yi Kuang,Shu-nan Tang,Kai Li,Yun Huang,Xue Qiao,Si-wang Yu,Yew-min Tzeng,Jen-Yu Lo,Min Ye
Ethnopharmacology relevance Antrodia camphorata (AC) is a rare and precious fungus indigenous to Taiwan used as a traditional medicine for the treatment of liver injury. Triterpenoids are the major bioactive constituents of A. camphorata and have been reported to possess hepatoprotective activities. To meet the increasing demand, artificial cultivation techniques have been developed. Aim of the study This study aims to evaluate the hepatoprotective activities of AC samples derived from different cultivation techniques and to dissect the main active triterpenoid compounds. Materials and methods The ethanol extracts of five batches of AC samples, including wild growing fruiting bodies, cutting wood culture fruiting bodies, dish cultures, cutting wood culture mycelia, and submerged fermentation mycelia were orally administered (50 mg/kg or 200 mg/kg) to ICR mice for 7 days. On the last day, CCl 4 (0.2%, 7 mL/kg, i.p.) was used to induce liver injury, and the activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined 24 h after the injection. Moreover, a HepG2 cell model treated with CCl 4 (0.35%) was used to screen the protective activities of 29 AC triterpenoids. After incubation for 6 h, viabilities of the cells were tested using MTS assay. The in vivo hepatoprotective activities of antcin B and antcin K were further studied on the mice model by ALT and AST tests and histopathologic examinations. To elucidate the mechanisms, the mRNA levels of iNOS , COX2, TNF-α and IL-1β, and the protein levels of NF-κB (p65/p-p65), iNOS and COX2 in liver tissues were determined. Results The wild growing or cutting wood culture fruiting bodies, and the dish cultures of AC showed more potent activities than the mycelia ( P <0.001). At 20 μM, 16 of 29 triterpenoids showed significant protective activities, increasing HepG2 cell viability from 46% of the CCl 4 group to >90%. Antcin B and antcin K could dose-dependently (10 or 50 mg/kg, 7 days, i.g.) decrease the serum levels of ALT and AST, and decrease the incidence of liver necrosis . The effects of 50 mg/kg of antcin K or antcin B were almost identical to those of 100 mg/kg silymarin . Furthermore, qRT-PCR and Western blotting analyses revealed they could down-regulate IL-1β, TNF-α, iNOS, COX-2 and NF- κ B in liver tissues at both transcriptional and translational levels. Conclusion The results indicate that cultivation techniques remarkably affect the hepatoprotective activities of AC. Antcin K and antcin B are the major hepatoprotective compounds of A. camphorata , and the mechanism is related with anti-inflammation. Given its high natural abundance and good oral absorption, antcin K could be a promising drug candidate for liver injury.
研究领域:
药理学
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中药学
·
肝脏病学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2020
·
IF 4.845
Shiping Zhu,Chunhua Luo,Weifeng Feng,Yan Li,Mujuan Zhu,Shengyun Sun,Xingwang Zhang
Arthritis remains the notion of a hard-to-treat disease that raises an area of unmet clinical need. The phytomedicine tripterine (Tri) and trace element selenium (Se) have been shown to be of anti-inflammatory activity. This study was devoted to develop nanomedicine containing Tri and Se used for fighting against arthritis via a coordination mechanism. Se-deposited Tri phytosomes (Se@Tri-PTs) were prepared by a melting-hydration/ in situ reduction technique and characterized by particle size, ζ potential , morphology, and entrapment efficiency ( EE ). The resultant Se@Tri-PTs were 126 nm around in particle size with an EE of 98.85%. Se@Tri-PTs exhibited a sustained drug release both in 0.1 M HCl and pH 6.8 PBS compared with Se-free phytosomes (Tri-PTs). The in vivo antiarthritic test demonstrated that Se@Tri-PTs could result in significant resolution of arthritis and decline of inflammatory factors. Phytosomes primely facilitated the transepithelial transport of Tri, while Se enhanced the antiarthritic efficacy of the phytomedicine synergistically. The present work provides a proof-of-concept for the combined therapy of arthritis using Tri and Se in the form of nanoparticles .
研究领域:
药理学
·
纳米医学
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产品关联
DOI
PubMed
高影响因子
SCIENTIA HORTICULTURAE
·
2024
·
IF 3.9
Xuan Wan,Xin Lin,Yun Zhang,Dengcan Luo,Junsen Peng,Hua Huang,Xiaochun Ding,Xiaoqing Dong
The occurrence of chilling injury is closely related to changes in wax. In this study, we investigated the relationship in plum fruit between wax presence (CK) and removal of epicuticular wax (REW) based on micromorphology and metabolomics. The results showed that wax reduced the incidence of chilling injury in plum fruit and slowed down the rate of loss of fruit firmness, and that the abundant wax structure not only protected the epidermal cells but also inhibited high-intensity respiration of the fruit. The correlation analysis between wax components and chilling injury illustrated that triterpene components were positively correlated with the occurrence of chilling injury. In summary, the present study revealed that wax components were involved in regulating chilling injury in plums, which provides a research idea to delay and reduce the occurrence of chilling injury in plums.
研究领域:
植物学
·
食品科学
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DOI
高影响因子
MOLECULES
·
2015
·Vol. 20
·期 4
·6273-6283
·
IF 2.416
Yun-Feng Zheng,Juan-Hua Wei,Shi-Qi Fang,Yu-Ping Tang,Hai-Bo Cheng,Tian-Lin Wang,Cun-Yu Li,Guo-Ping Peng
Two novel oleanane-type triterpene saponins, licorice-saponin P2 (1) and licorice-saponin Q2 (3), together with nine known compounds2,4–11, have been isolated from the water extract of the roots ofGlycyrrhiza inflata. The structures of these compounds were elucidated on the basis of spectroscopic analysis, including 2D-NMR experiments (1H–1H COSY, HSQC, HMBC and ROESY). Inin vitroassays, compounds2–4,6and11showed significant hepatoprotective activities by lowering the ALT and AST levels in primary rat hepatocytes injured by D-galactosamine (D-GalN). In addition, compounds2–4,6,7and11were found to inhibit the activity of PLA2with IC50values of 6.9 μM, 3.6 μM, 16.9 μM, 27.1 μM, 32.2 μM and 9.3 μM, respectively, which might be involved in the regulation of the hepatoprotective activities observed.Keywords:Glycyrrhiza inflate;genusGlycyrrhiza;triterpene saponin;hepatoprotective;phospholipase A2
研究领域:
药用植物化学
·
肝脏保护研究
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2018
·Vol. 19
·期 7
·2047
·
IF 3.687
Yao Li,Sheng Guo,Quanjin Ren,Dandan Wei,Ming Zhao,Shulan Su,Zhishu Tang,Jin-Ao Duan
Jujubae Fructus, the dried fruit ofZiziphus jujuba, has been used as Chinese medicine and food for centuries. Triterpenic acids have been found to be the major bioactive constituents inJujubae Fructusresponsible for their hepatoprotective activity in previous phytochemical and biological studies, while few pharmacokinetic studies have been conducted. To reveal the kinetics of the triterpenic acids under the pathological liver injury state, an established ultra-performance liquid chromatography coupled with a mass spectrometry method was applied for the simultaneous quantitation of seven triterpenic acids (ceanothic acid, epiceanothic acid, pomonic acid, alphitolic acid, maslinic acid, betulinic acid, and betulonic acid) in plasma samples of normal and acute liver injury rats induced by CCl4. The results showed that there were significant differences (p< 0.05) in the pharmacokinetic parameters of seven triterpenic acids between model and normal groups. The AUC0–tand AUC0–∞of epiceanothic acid (5227 ± 334 μg⋅h/L vs. 1478 ± 255 μg⋅h/L and 6127 ± 423 μg⋅h/L vs. 1482 ± 255 μg⋅h/L, respectively) and pomonic acid (4654 ± 349 μg⋅h/L vs. 1834 ± 225 μg⋅h/L and 4776 ± 322 μg⋅h/L vs. 1859 ± 230 μg⋅h/L, respectively) in model rats were significantly higher than those in normal rats, and the CLz/F of them were significantly decreased (0.28 ± 0.02 L/h/kg vs. 1.36 ± 0.18 L/h/kg and 19.96 ± 1.30 L/h/kg vs. 53.15 ± 5.60 L/h/kg, respectively). In contrast, the above parameters for alphitolic acid, betulinic acid and betulonic acid exhibited the quite different trend. This pharmacokinetic research might provide useful information for the clinical usage of triterpenic acids fromJujubae Fructus.Keywords:Ziziphus jujuba;triterpenic acids;pharmacokinetic study;acute liver injury
研究领域:
中药学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
PHYTOCHEMISTRY
·
2020
·
IF 3.044
Ming Zhou,Xiaogang Peng,Jia Zhou,Ye Liu,Xianggao Meng,Hanli Ruan
Six undescribed triterpenoids , schincarins A‒F, categorized as the preschisanartane (schincarin A), 3,4-secocycloartane (schincarins B‒D), 3,4; 9,10-disecocycloartane (schincarin E), and 3,4-secolanostane (schincarin F) classes, along with twelve known analogues, were obtained from the stems and leaves of Schisandra incarnata Stapf. Their structures were elucidated on the basis of extensive spectroscopic analyses. The absolute configuration of schincarin A was corroborated by analysis of the experimental ECD spectrum, and single-crystal X-ray diffraction with Mo K α irradiation. Schincarin A represented the first example of preschisanartane-type triterpenoid with two oxa-bridged hemiketals . Among the selected compounds which were evaluated for immunosuppressive activities, schincarin C and kadsudilactone A showed potential inhibition against ConA-induced T-cell proliferation with IC 50 values of 5.83 and 6.32 μ M, and LPS-induced B cell proliferation with IC 50 values of 10.21 and 11.49 μ M, respectively.
研究领域:
植物化学
·
药理学
·
有机化学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）