PROCESS BIOCHEMISTRY
·
2017
·
IF 2.497
Wen Qin,Chengjin Wu,Wei Song,Xiulai Chen,Jia Liu,Qiuling Luo,Liming Liu
In this study, an eco-friendly and high-efficient method for the bioconversion of phosphatidylserine (PS) from phosphatidylcholine (PC) and l -serine was developed. After the strain screening, the Streptomyces chromofuscus phospholipase D ( Sc PLD) was chosen and expressed in Escherichia coli BL21 with pET28a (+). After optimization, the maximal specific productivity and Sc PLD hydrolysis activity reached 9049.6 U/g and 10.6 U/mL, respectively, while induction with 2 g/L lactose, at 25 °C for 12 h. The maximal conversions of PC to PS was 93.4% with a final PS concentration of 106.2 g/L (53.1 g/L/h) under theoptimal conditions: 0.153 M PC in CPME, 2.3 M l-ser in sodium acetate buffer, 2.0 g lyophilized cells/mol PC, 100 mM Ca 2+ , 40 °C, and 2 h of incubation. After scale-up to 1 L, the conversions and yield maintained at 85.4% and 97.2 g/L. Therefore, the enzymatic process showed great potential for industrialization of PS.
研究领域:
生物化学
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酶工程
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工业微生物学
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DOI
高影响因子
CERAMICS INTERNATIONAL
·
2020
·
IF 3.83
Hong Zhou,Ying-Ying Jiang,Shuo Tan,Li-Jia Liu,Qian-Ting Yao,Yu-Jian Xia,Yun-Shan Fan,Jian-Ping Hu,Zi-Fei Zhou,Bing-Qiang Lu,Shi-Sheng He,Feng Chen
Biomineralization is a set of processes whereby living organisms generate hierarchically structured organic–inorganic materials. Studies on biomineralization not only elucidate the natural process of biomineral formation but also contribute to the development of functional biomaterials. However, the function of small molecules in the process has not been clarified. In this study, phosphoserine (PS) was used as a phosphorus source to prepare biomimetic minerals , and a new flower-like organic–inorganic complex of calcium PS (CaPS) was obtained. The CaPS samples were self-assembled plate-like crystals in the first 10 min. Then, over the next 5 h of the reaction, the plate-like crystals were broken down into fine granular nanoparticles . The chemical and thermal properties of CaPS were characterized, and the results indicated that the CaPS was or had a similar structure to Ca(C 3 H 6 NO 6 P)·H 2 O. X-ray absorption near edge structure (XANES) and extended X-ray absorption fine structure spectra (EXAFS) were obtained, which clearly revealed 6-fold-coordinated Ca-O bonds with lengths of 2.319 (±0.0147) Å, 2.358 (±0.0147) Å, and 2.435 (±0.0147) Å. Furthermore, the results of a CCK-8 assay indicated the high biocompatibility of CaPS. When the samples were co-cultured with mouse preosteoblast cells (MC3T3-E1), CaPS exhibited good bioactivity to promote the osteoblast differentiation of cells. Owing to its excellent chemical and biological properties, the as-prepared CaPS complex is promising for various biomedical applications.
研究领域:
材料科学
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生物材料
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生物医学工程
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DOI
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
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2019
·
IF 2.298
Yuwen Fan,Wenjun Zhu,Yonghong Hu,Wenge Yang,Qiang Xu,Xinxin Liu,Bin Heng
In the study, the solubility of l-serine were measured in eight common pure solvents and binary mixed solvents (acetonitrile + water, isopropanol + water, methanol + water, and acetone + water) from 278.15 to 333.15 K under atmospheric pressure by gravimetric method (acetone was measured from 278.15 to 328.15 K). The solubility of l-serine increases with increasing temperature, and the order in eight common pure solvents is water > acetonitrile > methanol > acetone > ethyl acetate > ethanol > n-butanol > isopropanol. In binary mixed solvents, the solubility of l-serine is dependent on temperature and solvent composition. Also, the solubility of l-serine increases with increasing temperature, and it decreases as the concentration of acetonitrile, isopropanol, methanol, or acetone increases at a constant temperature. The experimental data in eight pure solvents were fitted by the modified Apelblat model, Buchowski–Ksiazaczak λh model. The modified Apelblat equation, combined nearly ideal binary solvent/Redlich–Kister (CNIBS/R–K) model, and Jouyban–Acree model were used to correlate the experimental data in four binary solvents. Among the four models, the modified Apelblat model is more suitable with a higher accuracy than the other three models. The experimental data can be used to optimize the industrial manufacturing process of l-serine.
研究领域:
化学工程
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生物化学
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DOI
高影响因子
Catalysts
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2020
·Vol. 10
·期 9
·1057
·
IF 3.52
Jing Wang,Sheng Xu,Yang Pang,Xin Wang,Kequan Chen,Pingkai Ouyang
To achieve efficient bio-production of phospholipase D (PLD), PLDs from different organisms were expressed inE.coli. An efficient secretory expression system was thereby developed for PLD. First, PLDs fromStreptomycesPMF andStreptomyces racemochromogeneswere separately over-expressed inE.colito compare their transphosphatidylation activity based on the synthesis of phosphatidylserine (PS), and PLDPMFwas determined to have higher activity. To further improve PLDPMFsynthesis, a secretory expression system suitable for PLDPMFwas constructed and optimized with different signal peptides. The highest secretory efficiency was observed when the PLD * (PLDPMFwith the native signal peptide Nat removed) was expressed fused with the fusion signal peptide PelB-Nat inE. coli. The fermentation conditions were also investigated to increase the production of recombinant PLD and 10.5 U/mL PLD was ultimately obtained under the optimized conditions. For the application of recombinant PLD to PS synthesis, the PLD properties were characterized and 30.2 g/L of PS was produced after 24 h of bioconversion when 50 g/L phosphatidylcholine (PC) was added.Keywords:phospholipase D;phosphatidylserine;secretory expression;enzymatic catalysis
研究领域:
生物化学
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分子生物学
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DOI
高影响因子
APPLIED CLAY SCIENCE
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2016
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IF 2.586
Zongrong Wang,Wan Zheng,Zhenqian Zhang,Likun Chen,Zhongfei Zhang,Yong Li,Ning Ma,Piyi Du
A kaolinite-serine intercalation compound was synthesized via the guest displacement method. Serine molecules were intercalated into kaolinite to form kaolinite-serine through exchange between serine and the guest molecules ethylene glycol or water, which was inserted into the interlayer space of kaolinite beforehand. The thickness of kaolinite-serine particles decreased to 14 nm from 30 nm of raw kaolinite after intercalation. The interlayer distance of kaolinite reached approximately 1.13 nm after the intercalation of serine molecules. The serine molecules inserted in the interlayer space of kaolinite molecules were arranged in a flattened monolayer configuration and partially compressed into the tetragonal holes in the silicate layer. The activation energy Ea for releasing the pre-intercalated molecule before ultimate exchanging with serine determines the intercalation degree of serine into kaolinite. Higher Ea makes the expansion of kaolinite with pre-intercalated molecules more stable, which will facilitate the final intercalation of serine. The activation energy is 6.95 kJ/mol for ethylene glycol and 21.2 kJ/mol for water, respectively, resulting in serine intercalation degree of 45%, and 85% correspondingly. The kaolinite-serine intercalation compound is expected to be applied in the pharmaceutical and cosmetics fields.
研究领域:
材料科学
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化学
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生物医学工程
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DOI
高影响因子
Biotechnology Journal
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2023
·Vol. 18
·期 9
·2300027
·
IF 4.7
Jian-Miao Xu,Zhou-Sheng Wu,Ke-Ji Zhao,Zhi-Jie Xi,Liu-Yu Wang,Feng Cheng,Ya-Ping Xue,Yu-Guo Zheng
Background Biocatalytic production of L-phosphinothricin (L-PPT) is currently the most promising method. In this work, we use an Escherichia coli strain coexpressing of D-amino acid oxidase and catalase ( E. coli DAAO-CAT) to oxidation biocatalytic D-PPT to PPO, then use the second E. coli strain coexpressing glutamate dehydrogenase and formate dehydrogenase ( E. coli GluDH-FDH) to reduce biocatalytic PPO to L-PPT. Main Methods and Major Results We compared the effects of different concentrations of IPTG or lactose on protein expression and enzyme activity in 5 L fermenter. The best induction conditions for E. coli DAAO-CAT were 0.05 mM IPTG, induction for 18 h at 28°C. The specific enzyme activities of DAAO and CAT were 153.20 U g −1 and 896.23 U g −1 , respectively. The optimal induction conditions for E. coli GluDH-FDH were 0.2 mM IPTG, induction for 19 h at 28°C. The specific enzyme activities of GluDH and FDH were 41.72 U g −1 and 109.70 U g −1 , respectively. The 200 mM D-PPT was biocatalyzed by E. coli DAAO-CAT for 4 h with space-time yield of 9.0 g·L −1 ·h −1 and conversion rate of over 99.0%. Then 220 mM PPO was converted to L-PPT by E. coli GluDH-FDH for 3 h with space-time yield of 14.5 g·L −1 ·h −1 and conversion rate of over 99.0%. To our knowledge, this is the most efficient biocatalytic reaction for L-PPT production. Conclusions and Implications We found that IPTG has advantages compared with lactose in the enzyme activity and biomass of E. coli DAAO-CAT and E. coli GluDH-FDH, and IPTG is more environmentally friendly. Our data implicated that IPTG can replace lactose in terms of economic feasibility and effectiveness for scaled-up industrial fermentations. Graphical and Lay Summary A two-step enzymatic method was developed for the biological preparation of L-PPT from D-PPT. In the first step, we constructed a recombinant E. coli BL21(DE3)/pCDFDuet-DAAO-CAT that utilized D-amino acid oxidase (DAAO) to catalyze the conversion of D-PPT to PPO, and catalase to degrade the hydrogen peroxide (H 2 O 2 ) produced during the reaction. Subsequently, in the second step, we constructed another recombinant E. coli BL21(DE3)/pETDuet-LcGluDH-PseFDH that employed glutamate dehydrogenase (GluDH) for the reductive amination of PPO to L-PPT, with NADH supplied by formate dehydrogenase (FDH).
研究领域:
生物化学
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微生物学
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DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2024
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IF 7.1
Jia’nan Zhang,Zizhen Liu,Yihan Wang,Bo Yu
l-Homoserine is proven to be a promising precursor for the synthesis of l-phosphinothricin, which is a broad-spectrum herbicide with low toxicity. To enhance the sustainability of the process, the utilization of nonfood carbon sources for l-homoserine production is highly sought-after. Acetate, which can be captured from C1 gases, has the potential to become the most abundant feedstock for biomanufacturing. In this study, we systematically engineered the Escherichia coli strain to generate l-homoserine from acetate. The production of l-homoserine from acetate was initially achieved by deleting branch pathways, activating the glyoxylate shunt, and enhancing the synthetic pathway. The strain’s tolerance and acetate assimilation capabilities were strengthened through adaptive laboratory evolution. Reducing the flux toward gluconeogenesis further facilitated the production of l-homoserine. Finally, the engineered strain produced 15.96 g/L l-homoserine solely from CO2-derived acetate and formate in a 5 L fermentor via a fed-batch process. This study provides insights into the development of a low-carbon bioeconomy for the herbicide industry.
研究领域:
生物工程
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可持续发展
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合成生物学
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DOI
高影响因子
Journal of Infrared Millimeter and Terahertz Waves
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2020
·Vol. 42
·期 1
·64-75
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IF 1.765
Bian Yujing,Zhang Xun,Zhu Zhenqi,Wu Xiaodong,Li Xiang,Yang Bin
Terahertz (THz) spectroscopy is regarded as a professional technology to probe collective molecular vibrational modes and intensity of bio–molecules, which is potential to analyze amino acids and short peptides. In this study, THz spectroscopy is utilized to investigate the correlations of glycine (Gly), Serine (Ser), amino acid mixtures of Gly/Ser, and dipeptides (GS and SG) in the frequency range of 0.3–2.5 THz. The results manifest that the collective molecular vibrational intensity of Ser is larger than Gly, implying that the hydroxyl methyl group (–CH 2 –OH) on the side chain of Ser exhibits larger motions than that of –H in Gly. Additionally, the THz spectra of amino acid mixtures of Gly/Ser fit the linear addition of the individual amino acid absorption spectrum. Herein, the THz spectra of dipeptide GS and its isomer SG are distinguished according to the different collective molecular vibrations produced by the terminal groups on the carbon skeleton. Compared to physical amino acid mixtures of Gly/Ser, the dipeptides exhibit diverse THz spectra, indicating that the different collective vibrational modes are caused by peptide bonds and terminal groups. These solid evidences confirm that THz spectroscopy can be availably used to analyze the correlations of amino acids, amino acid mixtures, and dipeptides, as well as to identify dipeptide and its isomer from molecular level.
研究领域:
生物化学
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分子生物学
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光谱学
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DOI
高影响因子
RSC Advances
·
2013
·Vol. 3
·期 48
·25982-25988
·
IF 2.562
Tiefan Huang,Mo Zhang,Lihua Cheng,Lin Zhang,Man Huang,Qiuping Xu,Huanlin Chen
A polysulfone (PSF)-based hollow fiber affinity membrane for in vitro endotoxin elimination was explored and prepared successfully. This depended on the well-known hemocompatibility of PSF membrane. The PSF hollow fiber membranes were firstly activated by a chloromethylation reaction, and treated with ethylenediamine and glutaraldehyde successively. L-Serine (Ser) was finally immobilized on the surface of the membranes as the functional ligand. The modified membrane was confirmed by X-ray photoelectron spectroscopy, attenuated total reflectance Fourier transform infrared spectroscopy, and contact angle analysis. L-Ser density up to 8.4 μmol cm−2 on PSF membranes could be achieved. The adsorption performance was preliminarily studied by comparing the PSF membrane and PSF–Ser membrane in deionized water and sodium phosphate buffer, showing that the PSF–Ser membrane had improved endotoxin adsorption capacity with decreased Bovine Serum Albumin (BSA) adsorption. The PSF–Ser membrane was further investigated in human plasma, and showed excellent removal efficiency and specificity for endotoxin. These results demonstrate the promising application potential of this novel affinity membrane in sepsis therapy.
研究领域:
生物医学工程
·
材料科学
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DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2014
·Vol. 132
·期 1
·
IF 1.64
Xiaohai Bu,Yuming Zhou,Tao Zhang,Man He,Yongjuan Wang
Serine-based monosubstituted acetylene monomers were synthesized and polymerized with rhodium zwitterion catalyst in THF to afford optically active polyacetylene derivatives (LPA and DPA) and corresponding racemic polyacetylenes (RPA) with moderate molecular weights in good yields. All of the substituted polyacetylenes (SPA) were characterized by FT-IR, NMR, GPC, UV-Vis spectroscopy, circular dichroism (CD) spectroscopy, and TGA. LPA and DPA were soluble in common organic solvents and possessed single-handed helical conformation according to their intense Cotton effect and large specific rotations, while RPA presented random coiled polymer chain. The characterization results showed that helical structure of these SPAs was stabilized by intra- and intermolecular hydrogen bonding between the substituents which played a significant role in creating and maintaining the helix. Further, the infrared emissivity properties of them at wavelength of 8 to 14 μm were investigated at room temperature. Consequently, the LPA and DPA exhibited lower infrared emissivity values than RPA, which came down to 0.632 and 0.616. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132 , 41210.
研究领域:
有机化学
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材料科学
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光谱学
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DOI
高影响因子
Food & Function
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2020
·Vol. 11
·期 9
·8089-8098
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IF 4.171
Liuqin He,Yonghui Liu,Jing Long,Xihong Zhou,Sijing Zeng,Tiejun Li,Yulong Yin
Maternal dietary serine affects free amino acid content in milk and the antioxidant ability of progeny. However, whether maternal dietary serine has any effects on offspring performance in pigs and related metabolic consequences remains unknown. This study was conducted to investigate the effects of different levels of maternal dietary serine from late pregnancy to lactation on sow reproductive performance and offspring performance, and on the metabolome of milk and the serum of sows and their offspring. The results showed that sows fed a diet supplemented with 25% serine of the basal diet (L-Ser) had a higher litter weight, and higher average piglet weight at birth and aged 21 days when compared with sows fed the basal diet (CON). We found a large number of metabolites in both colostrum and milk that differed significantly between sows in the CON and L-Ser groups. Additionally, twenty metabolites differed in the serum of piglets aged 21 days between the CON and L-Ser groups. Most of these metabolites are involved in purine and pyrimidine metabolism, glutathione and taurine metabolism, as well as phospholipid and sphingolipid metabolism, which may contribute to the growth-promoting effects of serine on offspring. Our results imply that maternal serine has the potential to improve offspring outcomes.
研究领域:
动物营养学
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代谢生物学
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生殖生理学
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产品关联
DOI
PubMed
高影响因子
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
·
2018
·Vol. 48
·期 3
·993-1002
·
IF 5.5
Zhou Xihong,Zhang Yumei,Wu Xin,Wan Dan,Yin Yulong
Background/Aims: Early weaning often causes gut dysfunction. Since serine alleviates oxidative stress and inflammatory response which are accompany with early weaning, we conducted the study to explore whether serine improves intestinal function in early-weaned piglets. Methods: Twenty-eight weaned piglets (aged 21 d) were fed either a basal diet or a basal diet plus 0.2% serine. We determined the effects of dietary serine supplementation on intestinal morphology by hematoxylin and eosin staining, expression of tight junction proteins (TJPs) by immunoblotting and immunofluorescence, expression of inflammatory cytokines and apoptosis markers by RT-qPCR and the level of antioxidant enzymes with ELISA kits in early-weaned piglets. Results: Serine supplementation increased daily body weight gain while decreasing diarrhea incidence. Both the jejunum and ileum of serine-supplemented piglets showed regularly arranged villi and microvilli. Moreover, dietary serine increased TJP expression, and alleviated apoptosis, inflammation, and oxidative stress in the intestine of early-weaned piglets. Conclusion: Our findings suggest that serine has the potential for use as a feed additive to prevent gut dysfunction caused by weaning
研究领域:
动物营养学
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胃肠病学
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兽医学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）