Biomass Conversion and Biorefinery
·
2021
·Vol. 13
·期 2
·1199-1211
·
IF 4.987
Fahim Raana,Xiwu Lu,Jilani Ghulam,Ali Farasat
The inadequate sanitation, global warming, and dwindling natural oil reserves pose significant threats to global sustainable growth. With this objective, biomass availability for bioenergy production could be ensured alternately by exploring non-conventional plant species and algae. This idea was examined by growing divergent plants and algae species separately in horizontal subsurface flow constructed wetlands and raceway ponds, later collected their biomass for bioethanol determination. Through this approach, sewage was fed to constructed wetlands and raceway ponds as influent and passed through these cultivated systems for wastewater treatment. Although all the four plant and algae species showed efficient results in sewage treatment, however, Ipomoea aquatica and Spirogyra were statistically superior to the others and produced the highest biomass yield of 4.5 and 4.1 kg m −2 , respectively. For bioethanol production, alkali–autohydrolysis combined pretreatment followed by enzymatic hydrolysis with two successive incubation periods (12 and 24 h) were employed. Results suggested that Spirogyra biomass rendered greater carbohydrate and ethanol concentration (3.7 and 1.9 mg L −1 , respectively) during 24 hours of incubation, whereas Oryza sativa acquired similar carbohydrate concentration as Spirogyra but slightly lower bioethanol yield. This study postulates, Spirogyra is promising for wastewater treatment coupled with bioethanol production. Graphical abstract
Open raceway pond and constructed wetland coupled with wastewater treatment and bio-ethanol production
研究领域:
环境科学
·
生物能源
✓ 已验证
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2019
·
IF 6.044
Xiahui Wang,Shouqing Cui,Jiajun Hu,Xiubing Ma,Tian-Ao Zhang,Yiu Fai Tsang,Jixiang Li,Min-Tian Gao
The bioconversion of rice straw into ethanol can alleviate the energy crisis and solve problems related to waste treatment. In this study, the effect of soluble polysaccharides (SPs) produced during rice straw saccharification on the formation of extracellular matrices (EMs) by the yeast Saccharomyces cerevisiae was investigated. SPs were characterized by high-performance liquid chromatography (HPLC) and fourier transform infrared spectroscopy (FT-IR). SPs reduced the inhibition of alcohol dehydrogenase activity by phenolic acids (PAs) and regulated the intracellular redox state, resulting in higher ethanol production. The results of flow cytometry, confocal laser scanning microscopy, and atomic force microscopy indicated that PAs changed microbial morphology and caused damage in microbial cell membranes. The protective effect of SPs against cell membrane damage could be attributed to the synthesis of polysaccharide-dependent extracellular matrix, which maintained cellular integrity even under phenolic acid stress. These findings provide new strategies to improve pretreatment and saccharification processes.
研究领域:
生物化学
·
微生物学
·
能源生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Hossain M. Zabed,Suely Akter,Yuehui Tian,Mudasir A. Dar,Junhua Yun,Mei Zhao,Arthur J. Ragauskas,Jia Li,Xianghui Qi
This study evaluated microbial systems and technological approaches to configure the whole slurry co-fermentation process for ethanol biosynthesis from a novel stover system rich in sugars. Two approaches, namely separate and simultaneous hydrolysis and co-fermentation (SHCF and SSCF, respectively), were investigated using Escherichia coli monoculture and E. coli- yeast coculture. The SSCF with E. coli monoculture produced 32.75 g/L ethanol, representing only 44.87% yield, which left 65.13 g/L of total sugars unconverted and exhibited limited xylose consumption. Subsequently, a coculture-based SHCF significantly enhanced sugar consumption, leading to increase in ethanol yield and concentration to 66.94% and 48.86 g/L, respectively. Nevertheless, xylose utilization remained minimal due to the preference for glucose and the inhibitory effects of certain compounds. Thereafter, modification of the medium composition by supplementing betaine and sodium metabisulfite improved ethanol production to 53.18 g/L by reducing the toxic effects of inhibitors. Finally, a dual-phase SSCF (DP-SSCF) was explored by allowing the consumption of sugars from the pretreated slurry in the first phase, followed by concurrent cellulose hydrolysis and utilization of the resulting glucose in the second phase. This strategy increased ethanol titer to 63.14 g/L, with 84.3% yield and 0.88 g/L/h productivity.
研究领域:
生物工程
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
FEMS MICROBIOLOGY LETTERS
·
2021
·Vol. 368
·期 15
·
IF 2.742
Wu Liang,Wen Yongdi,Chen Wenying,Yan Tongshuai,Tian Xiaofei,Zhou Shishui
The reduced yields of acetaldehyde and fusel alcohols through fermentation by Saccharomyces cerevisiae is of significance for the improvement of the flavor and health of alcoholic beverages. In this study, the ADH2 (encode alcohol dehydrogenase) and THI3 (encode decarboxylase) genes of the industrial diploid strain S. cerevisiae XF1 were deleted. Results showed that single-gene-deletion mutants by separate gene deletion of ADH2 or THI3 led to a reduced production of the acetaldehyde or fusel alcohols, respectively. In the meantime, the double-gene-deletion mutant S. cerevisiae XF1-AT was constructed by deleting the ADH2 and THI3 simultaneously. An equivalent level of the ethanol production by the S. cerevisiae XF1-AT could be achieved but with the yields of acetaldehyde, isoamyl alcohol and iso-butanol reduced by 42.09%, 15.65% and 20.16%, respectively. In addition, there was no interaction between the ADH2 deletion and THI3 deletion in reducing the production of acetaldehyde and fusel alcohols. The engineered S. cerevisiae XF1-AT provided a new strategy to alcoholic beverages brewing industry for reducing the production of acetaldehyde as well as the fusel alcohols.
研究领域:
微生物学
·
发酵技术
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENERGY & FUELS
·
2023
·
IF 5.3
Zhihui Ling,Shang Wang,Zhongzhan Leng,Chengmei Li,Wenhui Zhang,Xinxin Kang,Xianghui Qi,Cunsheng Zhang
To improve the performance of ethanol production from food waste, a consolidation process involving both the addition of surfactant and cell immobilization was investigated. Results showed that the optimum glucose concentration was 200 g/L for ethanol fermentation using Saccharomyces cerevisiae as inoculum. With the addition of 0.5% Tween 80, the ethanol production was improved from 68.1 to 79.8 g/L, which was attributed to the increased mass transfer of cells provoked by Tween 80. In a cell-immobilized system, wheat straw was used as the matrix to carry the cells. A high ethanol production of 87.8 g/L was obtained with the sequential fermentation mode due to the high cell density on the cell carrier. The results of a mass balance calculation revealed that the ethanol production of food waste achieved 340.4 kg/t (on a dry basis), which provided an insight that food waste was a suitable substrate for ethanol production. The main cost was spent on energy consumption in the processes of food waste sterilization, hydrolysis, fermentation, and ethanol distillation, and measures for reducing the energy were suggested to improve the economic performance of ethanol production.
研究领域:
生物技术
·
环境科学
·
发酵工程
✓ 已验证
产品关联
DOI
高影响因子
RENEWABLE ENERGY
·
2024
·
IF 9
Xiangjun Guo,Yun Huang,Yunheng Tang,Ao Xia,Xianqing Zhu,Xun Zhu,Qiang Liao
Mixed bacterial syngas fermentation is a promising carbon-neutral method for producing acetic acid and ethanol. However, the bacterial community and metabolic pathways were different in the growth and ethanol production stages, whereas the activity of the bacterial community greatly depended on the pH of the culture. In this study, the effect of pH on syngas fermentation performance and microbial community composition under continuous gas supply was investigated. The mixed bacteria grew fastest at a pH of 7, while a constant pH of 6 led to the highest ethanol concentration of 5.15 g L −1 , which was 160 % higher than that at pH 7. Based on this, a two-step pH regulation method was proposed in this study to improve the ethanol production. Initially, the culture pH was maintained at 7 to create optimal growth conditions. Once bacterial density stabilized, it was then changed to a pH 6 to promote ethanol production. By doing this, compared with the control group, the maximum ethanol concentration (5.85 g L −1 ) increased by 148.9 %, the ethanol production rate (2.41 g L −1 d −1 ) increased by more than 8 times. This two-step pH regulation method ensured a high bacterial density and effectively stimulated ethanol production during syngas fermentation.
研究领域:
微生物学
·
生物技术
✓ 已验证
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2021
·
IF 5.645
Meishan Fan,Chenbiao Zhao,Xinyu Huang,Hongdan Zhang,Jun Xie
In this study, various combined pretreatment strategies using liquid hot water (LHW), 60 % ethanol, 1% H 2 SO 4 and 0.5 % NaOH together with different surfactants (PEG 6000, Triton X-100 and Tween 80) were evaluated to improve glucose production from sugarcane bagasse (SCB) during enzymatic hydrolysis. After pretreatment with PEG 6000-assisted 1% H 2 SO 4 , the highest glucose yield of 75.04 % was achieved. Pretreatments of SCB with various combinations of H 2 SO 4 (1–3%, w/v) and PEG 6000 (0–1.5 %, w/v) were further investigated for enhancing enzymatic hydrolysis and ethanol production. When 1.5 % PEG 6000 was introduced during pretreatment with 2 % H 2 SO 4 , the highest glucose yield of 86.14 % and ethanol concentration of 20.17 g/L were liberated, which were much higher than those obtained from pretreatment with 2% H 2 SO 4 alone. This increased digestibility and fermentability can be attributed to decreases in crystalline sizes and PEG 6000 adherence to SCB surfaces, which was confirmed by cellulase adsorption isotherms, FT-IR, XRD, and SEM analysis. In addition, prehydrolysate and the stillage remaining after ethanol production were sequentially digested to seek potential biomethane production and resulted in final methane yields of 264.05 mL and 387.77 mL per g of volatile solids, respectively. The feasibility of the process provids an environmentally friendly technology for an integrated biorefinery.
研究领域:
生物技术
·
可再生能源
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2015
·
IF 4.494
Hong Guo,Shaolan Zou,Boshi Liu,Rongxin Su,Renliang Huang,Wei Qi,Minhua Zhang,Zhimin He
Enzyme recycling by re-adsorption is one of the primary methods for reducing enzyme usage in lignocellulose conversion. This work proposes the combination of an engineered yeast strain that expresses β-glucosidase with enzyme recycling to reduce the amount of supplemented β-glucosidase in enzyme recycling experiments. Using the engineered strain, a slight increase in ethanol concentration was obtained after a 96-h fermentation of pretreated corncobs. Ethanol concentrations increased by 34.7% and 62.7% in the following two recycle rounds using the engineered strain compared with those using its parental strain without β-glucosidase addition. Furthermore, with the addition of β-glucosidase at 30 CBU/g cellulose, the ethanol concentration after two recycle rounds exceeded 90% of that observed in the first SSF round with the engineered strain at a high initial cellulase loading of 45 FPU/g cellulose.
研究领域:
生物技术
·
酶工程
·
发酵工程
✓ 已验证
DOI
PubMed
高影响因子
Journal of Physical Chemistry C
·
2021
·
IF 4.126
Zhaolong Xie,Menghuan Chen,Yangshen Chen,Anxiang Guan,Qing Han,Gengfeng Zheng
The direct conversion of methane to value-added chemicals at ambient conditions is a long-awaited perspective, and the electrocatalytic methane oxidation reaction (CH4OR) offers the potential of utilizing abundant natural gas and renewable energy sources. However, due to the competition of water oxidation and the overoxidation of methane, CH4OR has remained as a significant challenge. In this work, we developed Rh/ZnO nanosheets as an effective catalyst for electrochemical conversion of methane into ethanol. The highly dispersed rhodium nanoparticles provided surface sites for methane adsorption, and ZnO allowed presentation of abundant active oxygen species for methane activation. With a 0.6% Rh doping ratio, the Rh/ZnO nanosheets demonstrated an excellent ethanol production rate of 789 μmol·gcat–1·h–1 at 2.2 V versus reversible hydrogen electrode, corresponding to a Faradaic efficiency of 22.5% and an ethanol production selectivity of 85%, suggesting a promising means for methane utilization at ambient conditions.
研究领域:
化学工程
·
能源科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2022
·
IF 11.889
Xuelian Zhou,Chunlong Guan,Yexuan Xu,Shilong Yang,Chen Huang,Jiulong Sha,Hongqi Dai
The anatomical and histochemical characterization of pretreated substrates is essential for the further valorization of biomass during the biorefinery process. In this work, the benzenesulfonic acid (BA)-treated substrates were employed for simultaneous saccharification and fermentation (SSF) of ethanol for the first time. An ethanol yield of 50.36% was attained at 10% solids loading and 47.45 g/L of ethanol accumulated at 30 % solids loading. The dramatic improvements could result from the deconstruction of cell walls, which were evidenced by fluorescence microscope and confocal Raman microscopy spectra. Additionally, for a thorough comprehension of the inherent chemistry of lignin during the BA pretreatment, the changes in lignin structure features were identified for the first time by gel permeation chromatography (GPC) and nuclear magnetic resonance (NMR). In summary, this study tried to probe the possibility of BA-treated Miscanthus for the SSF process and unveiled the mechanism of the efficient BA pretreatment.
研究领域:
生物能源
·
植物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2014
·
IF 3.107
Renliang Huang,Ming Cao,Hong Guo,Wei Qi,Rongxin Su,Zhimin He
Pomelo peel is an abundant pectin-rich biomass waste in China and has the potential to serve as a source of fuels and chemicals. This study reports a promising way to deal with pomelo peel waste and to utilize it as raw material for ethanol production via simultaneous saccharification and fermentation (SSF). An integrated strategy, incorporating hydrothermal treatment, multienzyme formulation, and fed-batch operation, was further developed to enhance the ethanol production. The results show that hydrothermal treatment (120 °C, 15 min) could significantly reduce the use of cellulase (from 7 to 3.8 FPU g–1) and pectinase (from 20 to 10 U g–1). A multienzyme complex, which consists of cellulase, pectinase, β-glucosidase, and xylanase, was also proven to be effective to improve the hydrolysis of pretreated pomelo peel, leading to higher concentrations of fermentative sugars (36 vs 14 g L–1) and galacturonic acid (23 vs 9 g L–1) than those with the use of a single enzyme. Furthermore, to increase the final ethanol concentration, fed-batch operation by adding fresh substrate was employed in the SSF process. A final solid loading of 25% (w/v), which is achieved by adding 15% fresh substrate to the SSF system at an initial solid loading of 10%, produced 36 g L–1 ethanol product in good yield (73.5%). The ethanol concentration is about 1.73-fold that at the maximum solid loading of 14% for batch operation, whereas both of them have a closed ethanol yield. The results indicate that the use of the fed-batch mode could alleviate the decrease in ethanol yield at high solid loading, which is caused by significant mass transfer limitation and increased inhibition of toxic compounds in the SSF process. The integrated strategy demonstrated in this work could open a new avenue for dealing with pectin-rich biomass wastes and utilization of the wastes to produce ethanol.
研究领域:
生物技术
·
环境科学
·
能源科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2025
·
IF 9
Zhongcheng Yang,Yue Yang,Yunna Li,Junwen Qi,Zhigao Zhu,Yujun Zhou,Jiansheng Li
A novel hollow fiber membrane containing a carbon-coated CuO x (CuC-HFM) was prepared for in-situ CO 2 electrochemical reduction to ethanol during wastewater treatment. The ratio of Cu-based metal organic framework/carbon black of 1/1 was relatively optimal (Cu 1 C 1 -HFM) due to higher pure water permeance (24.62 L m −2 h −1 bar −1 ), better hydrophilicity (contact angle of 60.00°), and more ethanol yield (165.22 mg L −1 m −1 ) at −2.5 V with KOH as electrolyte. For control group (Cu 2 C 1 -HFM and Cu 1 C 2 -HFM), they were 76.50°/89.10°, 3.90/4.42 L m −2 h −1 bar −1 , 64.85/57.32 mg L −1 m −1 , respectively. The peaks of Cu (002) crystal plane, Cu 2+ electronic orbits, covalent bonds between C and O, and CuO x were appeared on prepared membrane. The CuO x could strongly adsorb CO and subsequently carbon shell could selectively produce HOCCH and HOCHCH, which were benefit for CO 2 electro reduction to ethanol. Therefore, the Cu 1 C 1 -HFM owned higher ethanol production (88.50 g L −1 m −2 h −1 ) during filtrating anaerobic supernatant. Meanwhile, the membrane could reject 97.2 % COD with only 14.7 % membrane flux loss. The membrane without bias suffered 27.1 % flux loss. This system gave a new thought for CO 2 electro reduction and the CuC-HFM had a promising employment in anaerobic bioreactor in the future.
研究领域:
环境科学
·
材料科学
·
电化学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）