Small
·
2021
·Vol. 17
·期 30
·2100244
·
IF 13.281
Yuhong Lin,Hongkun Zhang,Jingyun Feng,Bori Shi,Mengying Zhang,Yuexing Han,Weijia Wen,Tongyi Zhang,Yabing Qi,Jinbo Wu
An ideal anti-counterfeiting label not only needs to be unclonable and accurate but also must consider cost and efficiency. But the traditional physical unclonable function (PUF) recognition technology must match all the images in a database one by one. The matching time increases with the number of samples. Here, a new kind of PUF anti-counterfeiting label is introduced with high modifiability, low reagent cost (2.1 × 10 −4 USD), simple and fast authentication (overall time 12.17 s), high encoding capacity (2.1 × 10 623 ), and its identification software. All inorganic perovskite nanocrystalline films with clonable micro-profile and unclonable micro-texture are prepared by laser engraving for lyophilic patterning, liquid strip sliding for high throughput droplet generation, and evaporative self-assembling for thin film deposition. A variety of crystal film profile shapes can be used as “specificator” for image recognition, and the verification time of recognition technology based on this divide-and-conquer strategy can be decreased by more than 20 times.
研究领域:
材料科学
·
光学工程
·
化学工程
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Xiuqing Li,Hongfen Bian,Siming Yu,Wei Xiao,Jianying Shen,Caifeng Lan,Kenan Zhou,Caihong Huang,Lei Wang,Dan Du,Yuehe Lin,Yong Tang
Using an enzyme-linked immunosorbent assay (ELISA) and limited dilution methods to screen and clone antigen-specific hybridoma cells is extremely time-consuming and labor-intensive. This work features a simple and rapid cell surface fluorescence immunosorbent assay (CSFIA), designed for the detection and isolation of antigen-specific hybridoma clones. In this assay, antigens are first anchored to the hybridoma cell surface through a dual-functioning molecular Oleyl-PEG4000-NHS. Specific antibodies secreted from hybridoma cells are then captured by the antigens on the cell surface. Positive hybridoma cells are stained using a fluorescently labeled anti-mouse IgG-Fc antibody. After the addition of a methylcellulose semisolid medium, positive clones are easily picked using a pipet. These positive cell clones can be used to produce monoclonal antibodies after direct expansion. Using this method, positive hybridoma clones against both malachite green and porcine epidemic diarrhea virus are selected with high efficiency. Compared to the ELISA-based method, the CSFIA-based method achieved the capability of isolating >2-fold more hybridoma clones in <25% of the corresponding processing time. In brief, the CSFIA-based method is highly efficient and inexpensive with a simple and direct operation, which is an excellent candidate method for antigen-specific positive clone isolation in a monoclonal antibody preparation.
研究领域:
免疫学
·
分子生物学
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DOI
PubMed
高影响因子
TALANTA
·
2023
·
IF 6.1
Yilin Peng,Yaqi Jin,Di Sun,Zihui Jin,Qiyang Zhao,Yue He,Bining Jiao,Yongliang Cui,Yaohai Zhang
Fenpyroximate is a systemic phenoxy pyrazole acaricide applied worldwide to prevent and control various phytophagous mites and has high activity against young mites, with residues increasingly being found in ecological environment and agricultural products. To identify its residues, four haptens of fenpyroximate were designed and an indirect competitive enzyme-linked immunosorbent assay (icELISA) based on monoclonal antibodies (2G4C7) was developed. Because the icELISA had higher sensitivity to the hydrolysate ( Hapten A ) of fenpyroximate, a method for indirectly determining the concentration of fenpyroximate was established by measuring the content of Hapten A . The assay had a working range of 0.07–1.49 ng/mL and a half-maximal inhibitory concentration (IC 50 ) of 0.34 ng/mL. It showed average recoveries of 77.0%–105.4 %, 72.3%–106.4 % in citrus and apple samples, respectively. The icELISA and UPLC-MS/MS test results for samples of various citrus cultivars are remarkably consistent. These results and data represent the icELISA is suitable and applicable for detecting fenpyroximate residuals in fruit and vegetable samples.
研究领域:
分析化学
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环境科学
·
农业科学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Yaping Duan,Congmin Liu,Xiaowen Dou,Liu Wu,Hao Liu,Linan Shan,Shihai Yang,Jiaoyang Luo,Meihua Yang
Triptolide is a characteristic chemical component and quality marker of Tripterygium wilfordii Hook. F., a widely used traditional Chinese medicine with various pharmacological activities. Triptolide-14-succinate is a derivative of triptolide that can be combined with bovine serum albumin and ovalbumin to form an immunogen and coating antigen. Monoclonal antibody specific to triptolide-14-succinate conjugated with ovalbumin was produced and named mAb 2D10 1H8. An indirect competitive enzyme-linked immunosorbent assay (icELISA) was established by using mAb 2D10 1H8. The 50% inhibition concentration and working range of triptolide for the icELISA were 0.28 and 0.09–0.87 μg mL −1 , (based on 20–80% binding inhibition of mAb 2D10 1H8 to triptolide, R 2 = 0.9997, y = -0.571Logx + 0.246), respectively. The icELISA displayed crossreactivity values of 48.6, 20.5, 3.27, and < 1.00% for triptonide, triptophenolide, tripterifordin, and other analogs of triptolide, respectively. The average recovery of triptolide from the Tripterygium wilfordii samples as determined by icELISA ranged from 90.05 to 106.6%. Triptolide was detected in 38 batches of Tripterygium wilfordii samples by icELISA, which was validated by ultra-fast liquid chromatography combined with electrospray ionization tandem-mass spectrometry. In conclusion, the icELISA method established in the present study is applicable for rapid authentication of Tripterygium wilfordii and related products.
研究领域:
中药学
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免疫学
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分析化学
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产品关联
DOI
高影响因子
Biosensors-Basel
·
2022
·Vol. 13
·期 1
·22
·
IF 5.743
Lingyuan Xu,A. M. Abd El-Aty,Jing Zhao,Xingmei Lei,Xiuyuan Zhang,Yun Zhao,Xueyan Cui,Yongxin She,Fen Jin,Jing Wang,Maojun Jin,Bruce D. Hammock
In this study, a previously unreported 3-((4-(isopropylamino)-6-(methylthio)-1,3,5-triazin-2-yl) amino) butyric acid hapten was designed and synthesized. This maximized the exposure of the antigen-determinant isopropyl of prometryn to the immune system in animals to induce the production of anticipated highly specific anti-prometryn antibodies. The hapten has a molecular weight of 285.37 Da. The compound was confirmed by nuclear magnetic resonance hydrogen spectroscopy (1H NMR), nuclear magnetic resonance carbon spectroscopy (13C NMR), and high-resolution mass spectrometry (HRMS). By using the active ester approach, immunogens and coated antigens were created. Bovine serum albumin (BSA) was used as an immunogen, along with the successfully produced hapten, to immunize mice. The IC50value of mouse monoclonal anti-prometryn antibody (mAb) 7D4 (the quantity of analyte that generated 50% prometryn inhibition) was 3.9 ng/mL. The anti-prometryn mAb was of the IgG1 subclass. The IC20(80% binding level (B/B0) of prometryn)-IC80(20% binding level (B/B0) of prometryn) range of the anti-prometryn monoclonal antibody standard curve working range was 0.9–18.1 ng/mL. The prepared mAb has good characteristics because it can specifically recognize prometryn, and the cross-reaction rates for ametryn, desmetryn, and terbumeton were 34.77%, 18.09%, and 7.64%, respectively. The cross-reaction rate with the other seven triazines was less than 1%. The hapten structure proposed can serve as an additional tool for modulating selectivity in detecting triazines.Keywords:prometryn;hapten;monoclonal antibody;immunoassays
研究领域:
环境科学
·
生物化学
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DOI
PubMed
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2024
·
IF 1.7
Jiaxin Wang,Wenhua Shang,Shibei Shao,Jialin Zhang,Qiuyang Wu,Min He,Licai Ma,Jianzhong Shen,Zhanhui Wang,Kai Wen
Paxilline (PAX) is a tremorgenic mycotoxin that is thought to be associated with various syndromes in animals. To facilitate the determination of this toxin, a hapten was chemically synthesized and then respectively conjugated to Bovine Thyroglobulin (BTG) and Bovine serum albumin (BSA) for antibody production. Monoclonal antibody (mAb) 4F8 was finally obtained and used to establish an accurate and sensitive indirect competitive ELISA (icELISA). Various parameters that could impact the performance of icELISA were optimized, including the ion concentration, pH value and organic solvent. Under optimal condition, this assay exhibits an IC50 value as low as 0.84 µg L−1. The limit of detection (LOD) of the established icELISA method for PAX was 0.03–0.3 µg L−1. In conclusion, this study provided a rapid, sensitive, and high-throughput immunoassay for the detection of PAX in grains.
研究领域:
免疫学
·
食品科学
·
毒理学
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产品关联
DOI
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2019
·
IF 2.398
Xiujin Chen,Zhaozhou Li,Fengxia Sun,Xiaotian Cao,Yao Wang,Li Cao,Hongli Gao,Dong Gao,Yawen Wang
Tebuconazole is widely applied for controlling pathogenic fungi of crops and proved hazardous to human being. To better measure tebuconazole residues, an enzyme-linked immunosorbent assay for screening tebuconazole residues in aqueous samples was established using an innovative hapten (hapten1) designed via molecular model. The work concentration of calibration standard curves based on aqueous matrices ranged from 0.51 to 10.71 ng mL–1. The cross-reactivities of other analogs were less than 6%. The detection limits of the assay were in the range of 0.197–0.297 ng mL–1 for tebuconazole in water samples. The average recoveries of tebuconazole in the spiked water samples varied from 76.76% to 107.52% with the variation coefficient scope of 4.54–13.26%. The obtained immunoassay was verified by gas chromatography coupled to mass spectrometry with high correlation coefficients (r > 0.9868). Herein, the proposed immunoassay offers a cost-effective, convenient, reliable tool for the determination of tebuconazole residues in aqueous samples.
研究领域:
环境科学
·
分析化学
·
毒理学
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产品关联
DOI
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 82
·67093-67101
·
IF 3.84
Yuping Wei,Jiandong Xu,Liang Zhang,Yankai Fu,Xia Xu
The huge demand for the most promising biopharmaceuticals, monoclonal antibodies (mAb), has resulted in a need for more efficient and less costly downstream purification processes for mAb. The currently used Protein A Agarose is unattractive due to toxic ligand leakage and high cost. In this study, three novel small peptide ligands, DWHW, CEWW and HEYW, were designed based on the interactions with human immunoglobulin G (IgG) using molecular simulations. The effects of pH, ionic strength and flow rate on the binding capacities to IgG were investigated. The static and dynamic binding capacities were determined. The dissociation constant (Kd) of the DWHW resin was 1.1 × 10−5 M. The binding capacity of the DWHW resin was 24.5 mg ml−1, which is comparable to Protein A Agarose. The DWHW resin was able to purify IgG from cMEM and CHO cell culture supernatants with a purity of more than 95% under elution conditions of pH 9 (50 mM sodium borate buffer) and pH 3 (50 mM glycine–HCl buffer). The results indicate that the small peptide ligands, especially DWHW, can offer a potential alternative for mAb purification.
研究领域:
生物化学
·
生物工程
·
制药工程
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产品关联
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2021
·
IF 3.935
Xing Han,Fang-jie Cheng,Bin Di,Hui Xu,Min Song,Tai-jun Hang,Yu-Ting Lu
Zopiclone, a non-benzodiazepine hypnotic, is the first-line treatment for insomnia. The quality and stability of zopiclone tablets directly affects its efficacy and safety. However, the impurity investigation in zopiclone tablets remain incomplete. In this study, the accelerated and long-term stabilities of zopiclone tablets, as well as the stability characteristics under thermal and photolytic conditions were evaluated according to the ICH guidelines. In addition, a sensitive and specific LC-QTOF-MS method was developed for the separation and identification of all the impurities in zopiclone tablets and its stability test samples. Nine impurities were found in the test samples, five among them have not been reported before. Based on the accurate mass and elemental compositions of the parent and product ions obtained, the structures of all the detected impurities were identified. Combined with the formulation composition analysis and stability studies, the origins and the formation mechanisms of these impurities were elucidated. The obtained results are useful for the establishment of the optimum formulation, storage condition, manufacturing processes and quality control of zopiclone tablets.
研究领域:
药物化学
·
分析化学
·
药学
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DOI
PubMed
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 36
·22005-22013
·
IF 3.361
Qingbao Yang,Yanhua Qi,Jingming Zhou,Yumei Chen,Chao Liang,Zhanxiang Liu,Xiaoli Zhang,Aiping Wang
Fleroxacin (FLE) is a broad-spectrum fluoroquinolone antibiotic widely used in animal husbandry, veterinary medicine and aquaculture. Eating animal-derived foods with FLE residues can cause allergies, poisoning or drug resistance. The water-soluble QDs (CdSe/ZnS) and anti-FLE monoclonal antibody (mAb) were used to prepare a fluorescent probe by the method of N-(3-dimethylaminopropyl)-N′-ethylcarbodimide hydrochloride (EDC) activation. The fluorescent probe was characterized by dynamic light scattering (DLS). The better bioactivity and stability of the fluorescent probe was obtained under the pH value of 8.0, the molecule molar ratio of EDC (1 : 2000) and anti-FLE monoclonal antibodies (1 : 10). The control line (C line) and test line (T line) of a nitrocellulose (NC) filter membrane were sprayed with SPA (0.05 mg mL−1) and FLE-OVA (1.4 mg mL−1) solutions with optimal concentration, respectively. A novel method of fluorescent immunochromatographic assay based on quantum dots (QDs-ICA) in this work exhibited good accuracy, reproductivity and excellent specificity under the optimal experimental conditions. Compared with the traditional method for the visual detection of FLE, the developed QDs-ICA can successfully determine FLE residues in pork meat with a better cut-off value of 2.5 ng mL−1. The QDs-ICA could be adapted for the rapid preliminary detection of FLE residues in pork meat for the first time.
研究领域:
生物化学
·
兽医学
·
食品安全
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产品关联
DOI
PubMed
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2015
·
IF 0.986
Naifeng Xu,Liguang Xu,Wei Ma,Hua Kuang,Chuanlai Xu
In this study, a highly sensitive monoclonal antibody (McAb) against chloramphenicol (CAP) was successfully raised and characterised by indirect competitive enzyme-linked immunosorbent assay (ic-ELISA). Three antigens coupled with different proteins were synthesised and determined by the UV-vis method. After five rounds of immunisation, antisera were collected from six Balb/C mice and screened by ic-ELISA. The standard inhibition curve of one CAP McAb (labelled 6F11) showed the best sensitivity to CAP after optimisation of the principal working conditions. The half-maximal inhibition concentration (IC50) was calculated to be 0.01 ng/mL and the linear working range was from 0.0028 ng/mL to 0.040 ng/mL with a correlation coefficient of 0.996. This McAb showed high specificity, with negligible cross-reactivities detected towards CAP analogues (florfinicol, florfinicol amine, thiamphenicol). The recovery rate was 98.9–106.8% while the coefficient of variation was 4.45–6.05%. The IC50 value and other parameters obtained for this CAP McAb suggest that it is likely to have a promising future in further applications in combination with different analytical techniques.
研究领域:
免疫学
·
分析化学
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产品关联
DOI
高影响因子
SENSORS
·
2015
·Vol. 15
·期 2
·4291-4301
·
IF 2.245
Min Feng,Dezhao Kong,Wenbing Wang,Liqiang Liu,Shanshan Song,Chuanlai Xu
A rapid, simple, sensitive, and specific immunochromatographic test strip was developed for the detection ofPantoea stewartii subsp. stewartii(Pss) in corn seed which was soaked overnight and then centrifuged for precipitate re-dissolved as samples. A pair of sensitive monoclonal antibodies for the immunochromatographic test strip was generated by mice immunization and cell fusion. Under optimized conditions, the lower detection limit of the strips for Pss was 1 × 105cfu/mL both in 0.01 M phosphate buffer solution and corn seed samples, with no cross-reactivity with other common plant pathogens. The developed strip is useful and rapid for the detection of Pss in corn seed samples.Keywords:Pantoea stewartii subsp. stewartii(Pss);immunochromatographic test strip;monoclonal antibody
研究领域:
植物病理学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）