FOOD CHEMISTRY
·
2021
·
IF 7.514
Yantong Pan,Zhaopeng Wang,Changfei Duan,Leina Dou,Kai Wen,Zhanhui Wang,Xuezhi Yu,Jianzhong Shen
The two sensitive fluorescence quantitative immunochromatographic assays (FQICAs), background fluorescence quenching immunochromatographic assay (bFQICA) and time-resolved fluorescent immunochromatographic assay (TRFICA), play an important role increasingly in rapid detection technology for food safety. Amantadine (AMD), used extensively in virus infections in livestock and poultry, has been prohibited due to hazard concerns over public human health. Therefore, AMD was used as a model molecule in the FQICAs establishment and comparison based on the same bioreagents. The outstanding performance in technical parameters of the two FQICAs indicated that they could provide rapid, precise, reliable technical support for large-scale on-site screening for AMD detection. What’s more, the systematic and comprehensive comparison of the two FQICAs would give useful suggestions for scientists and users in monitoring the harmful compounds.
研究领域:
食品安全检测
·
免疫学
·
分析化学
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Jie Liu,Tongxu Gu,Rui sun,Jiaojiao Sun,Jian Yin,Pengli Bai,Huancai Yin
Classical gold nanoparticle-based colorimetric immunochromatographic assay (ICA) exhibits moderate performance, limiting their application in trace detection. In this study, perovskite-MSN nanocomposites with high quantum yield (74.7 %), a narrow full width at half maximum (29.5 nm) and excellent water stability were synthesized using in situ crystallization growth and a water-assisted strategy. Carboxyl-modified perovskite-MSN nanocomposites were employed to label anti-gliadin monoclonal antibody (mAb) and construct dual-mode fluorescent ICA (FICA) for determination of gliadin in milk and beef samples. Compared to the traditional AuNP-based colorimetric ICA strips, the CsM-H-based FICA demonstrated improved sensitivity, with a limit of detection (LOD) of 3.62 ng/mL in quantitative analysis. The CsM-H-based FICA strips exhibited average recoveries ranging from 90.2 % to 110.0 %, with coefficients of variation (CVs) between 3.3 % and 7.4 % for milk and beef samples. The ultra-stable perovskite-MSN nanocomposites provided the FICA strip with excellent stability, enabling storage for up to six months at room temperature. This study highlights the advantages of CsM-H-based FICA, including its rapidity, simplicity, and cost-effectiveness, as a promising POCT biosensor.
研究领域:
材料科学
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分析化学
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2019
·
IF 2.398
Shanshan Song,Steven Suryoprabowo,Liqiang Liu,Hua Kuang,Liguang Xu,Wei Ma,Xiaoling Wu
A highly sensitive, specific, and rapid immunochromatographic assay for halofuginone (HFG) has been developed. A well-characterized immunogenic conjugate of HFG with bovine serum albumin was produced using the N,N″-Carbonyldiimidazole method. A sensitive monoclonal antibody specific for HFG was then generated by immunizing BALB/c mic with the HFG conjugate. The antigen, HFG-CDI-ovalbumin and goat anti-mouse IgG were attached to a nitrocellulose membrane as the test line and the control line, respectively to produce an immunochromatographic test strip for the rapid detection of HFG in milk. Under optimized conditions, the detection limits of the semi-quantitative test strip for HFG were as low as 50 ng/mL in 0.01 M PBS (pH 7.4) and 100 ng/mL in milk. All the results were obtained within 5 min. The newly developed immunochromatographic assay provides a sensitive, rapid, and specific procedure for on-site screening for HFG.
研究领域:
分析化学
·
免疫学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2020
·Vol. 10
·期 47
·28041-28048
·
IF 3.119
Caixia Liu,Baiping Mao,Vanessa Martinez,Xiaojian Chen,Yanan Li,Lingyun He,Sian Chen,Xiaoling Guo,Xian Shen,Xiandan Bao,Haifa Shen,Stefania Lenna,Pinyi Qian,Lingzhi Wu,Chao Li
The outbreak of new coronavirus disease (COVID-19) has quickly spread all over the world. Real time reverse transcriptase polymerase chain reaction (rRT-PCR) for nucleic acid detection has become the standard method for clinical diagnosis of COVID-19 infection. But these rRT-PCR tests have many inherent limitations, and carry a high false negative rate. It is an urgent to develop a method to accurately identify the vast infected patients and asymptomatic viral carriers from the population. In this article, we present the principle and procedure of developing a colloidal gold immunochromatographic assay (GICA) for rapid detection of COVID-19-specific antibodies. The detection kit can be used to detect immunoglobulin M (IgM) and IgG of COVID-19 in human blood samples within 15 minutes, and to identify different stages of viral infection. Test results can be digitalized using an office scanner and a FiJi software with appropriate confidence interval (CI) setting. Based on analysis from 375 samples, we calculated that overall sensitivity and specificity of the assay were 95.85% and 97.47%, respectively. Compared with rRT-PCR, this assay has many advantages including convenience and rapid detection. The detection kit can be widely used in hospitals, clinics and laboratories for rapid screening of both symptomatic and asymptomatic COVID-19 carriers in large scale.
研究领域:
医学
·
病毒学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS
·
2015
·Vol. 15
·期 2
·4291-4301
·
IF 2.245
Min Feng,Dezhao Kong,Wenbing Wang,Liqiang Liu,Shanshan Song,Chuanlai Xu
A rapid, simple, sensitive, and specific immunochromatographic test strip was developed for the detection ofPantoea stewartii subsp. stewartii(Pss) in corn seed which was soaked overnight and then centrifuged for precipitate re-dissolved as samples. A pair of sensitive monoclonal antibodies for the immunochromatographic test strip was generated by mice immunization and cell fusion. Under optimized conditions, the lower detection limit of the strips for Pss was 1 × 105cfu/mL both in 0.01 M phosphate buffer solution and corn seed samples, with no cross-reactivity with other common plant pathogens. The developed strip is useful and rapid for the detection of Pss in corn seed samples.Keywords:Pantoea stewartii subsp. stewartii(Pss);immunochromatographic test strip;monoclonal antibody
研究领域:
植物病理学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 36
·22005-22013
·
IF 3.361
Qingbao Yang,Yanhua Qi,Jingming Zhou,Yumei Chen,Chao Liang,Zhanxiang Liu,Xiaoli Zhang,Aiping Wang
Fleroxacin (FLE) is a broad-spectrum fluoroquinolone antibiotic widely used in animal husbandry, veterinary medicine and aquaculture. Eating animal-derived foods with FLE residues can cause allergies, poisoning or drug resistance. The water-soluble QDs (CdSe/ZnS) and anti-FLE monoclonal antibody (mAb) were used to prepare a fluorescent probe by the method of N-(3-dimethylaminopropyl)-N′-ethylcarbodimide hydrochloride (EDC) activation. The fluorescent probe was characterized by dynamic light scattering (DLS). The better bioactivity and stability of the fluorescent probe was obtained under the pH value of 8.0, the molecule molar ratio of EDC (1 : 2000) and anti-FLE monoclonal antibodies (1 : 10). The control line (C line) and test line (T line) of a nitrocellulose (NC) filter membrane were sprayed with SPA (0.05 mg mL−1) and FLE-OVA (1.4 mg mL−1) solutions with optimal concentration, respectively. A novel method of fluorescent immunochromatographic assay based on quantum dots (QDs-ICA) in this work exhibited good accuracy, reproductivity and excellent specificity under the optimal experimental conditions. Compared with the traditional method for the visual detection of FLE, the developed QDs-ICA can successfully determine FLE residues in pork meat with a better cut-off value of 2.5 ng mL−1. The QDs-ICA could be adapted for the rapid preliminary detection of FLE residues in pork meat for the first time.
研究领域:
生物化学
·
兽医学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Yan Gao,Junpeng Shi,Chen Wu,Longlong Cao,Lin Liu,Jinyuan Wang,Xiaofang Luo,Xiao Zhang,Yun Zhang
Immunochromatography is a commonly used immediate detection technique, using signal labels to generate detection signals for rapid medical diagnosis. However, its detection sensitivity is affected by background fluorescence caused by the excitation light source. We have developed an immunochromatographic test strip using Zn2GeO4:Mn2+ (ZGM) persistent luminescent nanoparticles (PLNPs) for immediate fluorescence detection and highly sensitive persistent luminescence (PersL) detection without background fluorescence interference. ZGM emits a strong green light when exposed to ultraviolet (UV) excitation, and its green PersL can persist for over 30 min after the excitation light is turned off. We modified the surface of ZGM with heparin-binding protein (HBP) antibodies to create immunochromatographic test strips for the detection of HBP as the target analyte. Under UV excitation, the chromatography test paper can be visually observed at concentrations as low as 25 ng/mL. After the excitation light source is switched off, PersL can achieve a detection limit of 4.7 ng/mL without background interference. This dual-mode immunochromatographic detection, based on ZGM, shows great potential for in vitro diagnostic applications.
研究领域:
生物医学工程
·
诊断学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2018
·
IF 2.568
Jiajia Sun,Liqiang Liu,Shanshan Song,Gang Cui,Hua Kuang
The problem of waste oil is a food safety issue that requires urgent resolution. In this study, a novel hapten was designed for the rapid detection of capsaicinoids, a marker of waste oil, and a monoclonal antibody (MAb) 5D7 was generated that is specific for three major capsaicinoids: capsaicin (CPC), dihydrocapsaicin (DCPC), and N-vanillylnonanamide (N-V). The half maximal inhibitory concentrations of MAb 5D7 for CPC, DCPC, and N-V were 1.12, 0.8, and 0.87 ng/mL, respectively. An immunochromatographic strip test based on MAb 5D7 was established for these three capsaicinoids, with visual inspection limits of <1.0 ng/mL, which satisfies the requirement for the rapid on-site inspection of waste oil.
研究领域:
食品安全
·
免疫学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Shuyue Ren,Qiaofeng Li,Jingyi Wang,Bingyan Fan,Jialei Bai,Yuan Peng,Shuang Li,Dianpeng Han,Jin Wu,Jiang Wang,Kang Qin,Zunquan Zhao,Baoan Ning,Tie Han,Huanying Zhou,Zhixian Gao
A rapid and ultrasensitive method for colourimetric/photothermal dual-readout detection was developed using an 808 nm NIR laser and a thermal imaging app on mobile phone. Norfloxacin was used as a model contaminant to demonstrate this universal rapid detection method. It is innovatively, to use the advanced two-dimensional material black phosphorus as a colourimetric/photothermal reagent for the first time. The samples were added to the strip, and the analytes were selectively captured on the conjugate pad by monoclonal antibody-modified magnetic/upconversion nanocomposites . The samples flowed through the strips by capillary action until reaching the control line, where immune complex formation occurred due to the presence of secondary antibody. The added black phosphorus could be captured by the the antigens which were directly exposed to the test line and a brown band could be observed by naked eye. Upon illumination by NIR light for 1 min, the real-time temperature is obtained for quantitative analysis through the thermal imaging performed by mobile phone camera. This method can achieve the detection of norfloxacin in water samples within 20 min, and the detection limits of colorimetric and photothermal readout can reach 45 pg mL −1 . Compared with conventional strips, this method provided an increased sensitivity by about two orders of magnitude, with a integrated portable laser and a mobile phone. It is a valuable method for rapid detection and can be applied to other environmental contaminants as well.
研究领域:
环境科学
·
分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF RAMAN SPECTROSCOPY
·
2020
·Vol. 51
·期 4
·642-648
·
IF 2
Wei Li,Xiaozhou Li,Tianyue Yang,Xingjia Guo,Youtao Song
An immunochromatographic assay (ICA) based on surface-enhanced Raman scattering (SERS) method was developed for the detection of morphine in this study. Indirect competitive immunoadsorption was adopted in the ICA due to the small molecular weight of morphine. Antibody modified gold nanoparticles in the ICA were labeled with Raman reporters and were used as the SERS enhancement substrate. By measuring the feature peaks of Raman reporter MBA at 1,078 cm −1 , a quantitative estimation of morphine can be obtained. The limit of detection of this ICA-SERS method was 2.4 × 10 −4 ng/ml for morphine, which was four orders of magnitude lower than the visual method. This study demonstrated that the proposed ICA-SERS method is a good candidate for the rapid and accurate detection of illicit drugs in saliva. All the analyses in this paper were carried out in a Microsoft (MS) Excel platform using embedded R functions through Excel BERT add-in.
研究领域:
分析化学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
BIOMEDICAL CHROMATOGRAPHY
·
2023
·Vol. 37
·期 8
·e5656
·
IF 1.8
Minmin Shen,Haiying Gu,Kai Deng,Zhongpeng Li,Yumei Yuan,Yangjin He,Yin Jin,Nengming Lin
Vancomycin (VAN) has a narrow therapeutic window and variable pharmacokinetic properties, which require timely monitoring of plasma concentration to adjust the dosage for better effectiveness. A sensitive and quantitative immunochromatographic method was developed to detect VAN in plasma. The linear response range of the method to detect plasma VAN was 1.25–50 μg/mL. The intra- and inter-assay coefficients were 4.68% and 8.81%, respectively, while the recovery was 89.10%–98.32%. The clinical comparative experiment results demonstrated a good correlation (r > 0.90) between the fluorescent immunochromatographic strip test and the mass spectrum. In this study, a simple, rapid, and high-sensitivity immunochromatographic method was established for detecting VAN, which helped establish the basis for further application of monitoring plasma concentration.
研究领域:
临床药理学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2022
·
IF 3.268
Kemeng Qin,Mingyue Ding,Chensi Zhang,Xijie Zhang,Yexuan Mao,Meng Dang,Zizhe Li,Youyi Wang,Shaohui Zhang,Yuhang Sun,Zhibin Zhang,Gaiming Zhao,Yu Li,Qian Li,Xiya Zhang
To improve the detection sensitivity of neomycin (NEO) in milk, we produced a sensitive monoclonal antibody (mAb) against NEO and developed a lateral flow immunoassay based on amorphous carbon nanoparticles (ACNPs-LFA). First, we conjugated NEO to carrier protein to prepare mAbs. We obtained six mAbs: mAb 1C6, mAb 1D3, mAb 2D3, mAb 4D5, mAb 5D1, and mAb 5H1. We characterised the mAbs by indirect competitive enzyme-linked immunosorbent assay and selected the most sensitive mAb based on the half-maximal inhibitory concentration (IC50). We selected mAb 4D5 (IC50 = 0.15 ng/mL) for the development of LFA. MAb 4D5 was labelled with ACNPs (ACNPs-mAb 4D5) by electrostatic absorption. Under optimised conditions, 5.4 μg mAb 4D5 coupling with 1 mL ACNPs, NEO-OVA at concentration of 80 μg/mL, 3 μL ACNPs-mAb 4D5 were used to develop LFA. The cut-off value was 8 ng/mL. Therefore, our developed ACNPs-LFA is suitable for on-site detection of NEO residues.
研究领域:
食品安全
·
免疫学
·
分析化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）