ACS Applied Materials & Interfaces
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2019
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IF 8.456
Lihua Chen,Mingchao Liu,Yan Tang,Chuangfu Chen,Xingxing Wang,Zhiqiang Hu
Gold magnetic particles as a new carrier of disease diagnosis probes have attracted wide attention but encountered a bottleneck. That is, the interfacial properties of gold magnetic particles are susceptible to the influence of nonspecific biological molecules in actual diagnostic samples. Here, a novel nanoparticle made by the covalent attachment of polyethyleneimine and hyperbranched polyether polyol onto the gold shell surface of a magnetic bead demonstrated not only low fouling properties but also excellent stability in a variety of external environments, especially in complex biological systems. Most importantly, in its application as the probe for sensitive and selective fluorescence detection of high-risk human papillomavirus (HPV) genotypes 18, 16 in buffer, even in 100% serum, a good linear correlation with the concentration of HPV18/16 target DNA ranging from 5 nM to 1 μM was shown with the low detection limits. To our knowledge, this is one of the few successful examples of direct application of magnetic beads to the detection of disease markers in whole serum, suggesting that this material has good commercial potential and value.
研究领域:
生物医学工程
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分子诊断学
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材料科学
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DOI
高影响因子
ANALYST
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2021
·Vol. 147
·期 2
·282-292
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IF 4.616
Chenhui Wu,Yunkang Ma,Yingxia Zhou,Wenjie Yang,Lihua Chen
Biofouling is a nuisance in the practical applications of biosensors, which seriously affects the reliability and accuracy of detection. The utilization of antifouling interface materials is a promising option for mitigating biofouling. Only highly accumulated antifouling polymeric surfaces tend to offer “zero” nonspecific protein adsorption. Herein, superior antifouling coatings based on chondroitin sulfate (CS) were prepared by the NH2-MIL-53 (Al) assisted strategy. This is a novel design to improve the antifouling property of material by taking advantage of the high specific surface area of the three-dimensional MOF to increase the accumulation degree of antifouling functional groups per unit area. And the related chemical technology is simple and easy to operate. As expected, this novel CS-loaded MOF demonstrated an excellent antifouling performance in various biological samples, even in 100% goat serum. Only 8.48% changes of differential pulse voltammetry (DPV) were found. Furthermore, this antifouling interface material is successfully applied for the specific detection of the tuberculosis (TB) gene in undiluted biofluids. This developed TB biosensor showed a high analytical performance with a wide linear range (1.00 × 10−16 M to 1.00 × 10−11 M) and a low detection limit, indicating that it may open new avenues for direct biosensing of disease markers for clinical samples.
研究领域:
生物传感器
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材料科学
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分析化学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2018
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IF 8.173
Shuli Lv,Jinliang Sheng,Shiyi Zhao,Mingchao Liu,Lihua Chen
It is a novel competitive challenge for electrochemical biosensor to directly, rapidly and ultrasensitively detect the disease markers in the whole serum due to biofouling caused by the complexity of actual samples. In this paper, poly (ethylene glycol) (PEG) and hyaluronic acid (HA) were utilized to modify Fe 3 O 4 @Au nanoparticles (NPs). Based on the successfully preparation and characterization of Fe 3 O 4 @ [email protected] @HA NPs with TEM , SEM, XRD, FTIR and EDS , respectively, a novel immunosensor of brucellosis with high selectivity , sensitivity and almost perfect protein-resistant properties in various external environments, especially, in complex biological systems was fabricated. More importantly, this immunosensor is capable of assaying brucellosis antibody in 100% serum without suffering from any significant biological interference. In addition, a wide linear response range from 10 –15 g mL −1 to 10 –11 g mL −1 towards antibody in 100% serum and a low limit of detection (LOD) of 0.36 fg mL −1 (3σ, n = 13) are demonstrated, which indicates that this immunosensor has a promising potential in clinical diagnosis.
研究领域:
生物传感器
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纳米技术
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分析化学
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临床诊断
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2020
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IF 5.977
Shiyi Zhao,Yingxia Zhou,Lai Wei,Lihua Chen
Based on the successful design and preparation of Fe 3 O 4 @ [email protected] glycol (PEG)@chondroitin sulfate (CS) nanoparticles , a novel electrochemical biosensor was elaborately developed for MO determination by the drop coating method. This biosensor demonstrates ultra-low fouling properties in complex biological systems, especially in whole serum. Only 2.40% of the differential pulse voltammetry (DPV) signal changes are shown in the resistance test of electrode interface to 100% goat serum. The constructed biosensor also presented a good selectivity (DNA samples for M1, M2 and M3give a response around 14.2–21.4%of that obtained by the target analyte), reproducibility (the relative standard deviation (RSD) is only 4.82%) and storage stability (93.2% of the initial response is maintained after 20 day). Moreover, this biosensor exhibits high analytical performance with a wide line arranges (10 −17 M ∼ 10 −12 M) in buffer, even in 100% goat serum. In both cases, a low limit of detection (LOD) of 3.3 aM(S/N = 3)is showed. To our knowledge, a few examples reported the application of electrochemical biosensors to the direct ultrahigh sensitive determination of MO, especially in whole serum.
研究领域:
电化学
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生物传感器
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分析化学
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微生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）