SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2017
·
IF 2.536
Longhua Ding,Hongmei Yang,Shenguang Ge,Jinghua Yu
A simple and effective fluorescent assay for the determination of vitamin B 12 was developed. In this study, carbon dots (CDs) were prepared by one-pot hydrothermal method and directly used as a fluorophore in the inner filter effect (IFE). Both of the maximum absorption peak of vitamin B 12 and excitation maxima of CDs are located at 360 nm, hence, the excited light of CDs can be absorbed by vitamin B 12 , resulting in the fluorescence reduction of CDs. And the fluorescence intensity of CDs decreases with the increasing concentration of vitamin B 12 . This IFE-based sensing strategy shows a good linear relationship between the normalized fluorescence intensity and the concentration of vitamin B 12 ranging from 0 to 60 μM, with a limit of detection (LOD) of 0.1 μM at a signal-to-noise ratio of 3. Furthermore, this proposed approach was successfully applied to vitamin B 12 sensing in injections. This IFE sensing platform based on various fluorescent nanomaterials has a high promise for the detection of other biomolecules due to its inherent convenience.
研究领域:
分析化学
·
生物医学
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DOI
PubMed
高影响因子
ACS Omega
·
2019
·
IF 2.584
Shan Huang,Erli Yang,Jiandong Yao,Xu Chu,Yi Liu,Yue Zhang,Qi Xiao
Detection of cholesterol and uric acid biomarkers is of great importance for clinical diagnosis of several serious diseases correlated with their variations in human blood serum. In this study, a new kind of well selective and highly sensitive ratiometric fluorescent probe for cholesterol and uric acid determination in human blood serum was innovatively developed on the basis of the inner filter effect (IFE) process of nitrogen, cobalt co-doped carbon dots (N,Co-CDs) with 2,3-diaminophenazine (DAP). DAP was the oxidative product during the oxidation reaction between o-phenylenediamine and H2O2. Fluorescent magnetic N,Co-CDs possessing blue emission and magnetic property were prepared through a facile one-pot hydrothermal strategy by using citric acid, diethylenetriamine, and cobalt(II) chloride hexahydrate as precursors. N,Co-CDs exhibited good ferromagnetic property and excellent optical properties even in extremely harsh environmental conditions, implying the huge potential applications of such N,Co-CDs in biological areas. On the basis of the IFE process between N,Co-CDs and DAP, N,Co-CDs were applied to establish ratiometric fluorescent probes for the indirect detection of cholesterol and uric acid that participated in enzyme-catalyzed H2O2-generation reactions. The established IFE-based fluorescent probes exhibited relatively low detection limits of 3.6 nM for cholesterol and 3.4 nM for uric acid, respectively. The fluorescent probe was successfully utilized for the determination of cholesterol and uric acid in human blood serum with satisfying results, which provided an informed perspective on the applications of such doped CDs to explore the specific and sensitive nanoprobe in disease diagnoses and clinical therapy.
研究领域:
生物化学
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临床医学
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纳米材料科学
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DOI
PubMed
高影响因子
TALANTA
·
2019
·
IF 4.916
Jie Ma,Lei Bian,Lei Zhao,Xiaotong Feng,Lizhi Zhao,Zhaoyan Wang,Qiaosheng Pu
Low-cost and nontoxic fluorescent reagents are important for on-site analysis performed by non-professionals. In this work, a cheap and widely available food additive , caramel was used as an effective fluorescence reagent for the sensitive detection of 2,4,6-trinitrophenol (TNP), 2,4-dinitrophenol (DNP) and 4-nitrophenol (4-NP). Through a simple dialysis, fluorescent components of a commercial caramel were harvested with a yield as high as 60%. The structural characterization demonstrated that the fluorescent components were dehydrated oligomers of carbohydrates. Their fluorescence can be quenched by these nitrophenols . The quenching mechanism was speculated as inner filter effect. At pH 8, a linear range of 0.2–22 μM and a detection limit of 90 nM could be achieved for TNP. Based on the difference of their quenching efficiency at different pHs, TNP, DNP and 4-NP can be simultaneously determined by solving the linear equations obtained at pH 3, 5 and 8. Successful detection of these nitrophenols in the water and soil samples was performed with relative standard deviations of 1.1–5.9% and recoveries of 95–108% of spiked standards.
研究领域:
分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Ciyang Zhang,Keren Lu,Linrui Li,Wu Lei,Mingzhu Xia,Fengyun Wang
Oxytetracycline hydrochloride (OTC) is a commonly used over-the-counter antibiotic, valued for its potent antibacterial properties. However, the inappropriate and excessive use of OTC can result in the accumulation of the drug in both the environment and human body, causing significant harm to ecosystems and human health. Therefore, the development of a fast and sensitive method for detecting OTC is of great significance. Lanthanide metal–organic frameworks (LnMOFs) can effectively excite lanthanide metals to emit long-lifetime, narrow and stable fluorescence based on the antenna effect, but their application in fluorescence sensing is rarely reported. In this work, a strongly fluorescent material Tb-MOF was synthesized by a facile solvothermal method using 1,2,4,5-phenylenetetracarboxylic acid (H 4 btec) and 1,10-phenanthroline (1,10-phen) as organic ligands and lanthanide metal Tb as the luminescent center. Tb-MOF is a stable material in water and shows excellent linearity with OTC in the concentration range of 0 ∼ 70 μM, with low detection limit (0.12 μM) and luminescence color transition from bright green to dark green during the detection process. X-ray diffraction, UV–vis absorption and fluorescence lifetime analyses revealed that the fluorescence quenching of Tb-MOF by OTC is caused by the inner filter effect in static quenching. Test strips for OTC detection were successfully prepared using Tb-MOF. These strips are not only low cost and easy to prepare but can also be used as portable sensing devices that can be easily distinguished by the naked eye during OTC testing. This study not only presents a fluorescent probe for the detection of OTC in water but also offers a practical method for converting fluorescent luminescent materials into functional devices for OTC detection.
研究领域:
分析化学
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环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
LUMINESCENCE
·
2016
·Vol. 31
·期 8
·1448-1455
·
IF 1.452
Lingcan Kong,Keyu Lu,Guangyuan Ma,Yuyang Yao,Xia Ling,Wenwei Liu
One strong fluorescent Bodipy-containing derivative was synthesized and characterized using 1 H NMR, electrospray ionization mass spectrometry and elemental analysis. Its electrochemical and photophysical properties were investigated. In addition, the Bodipy derivative could be used as an on–off fluorescent probe for the detection of Fe 3 + ions based on the inner filter effect because the absorption band of the Fe 3 + ion overlaps the excitation band of Bodipy very well upon irradiation with UV light. Furthermore, the Bodipy-based sensor has obvious advantages including simplicity, rapid response, high selectivity, sensitivity and a detection limit of 1.2 μ mol/L, and has been demonstrated in real water samples including tap water, mineral water and water from Lake Tai. Moreover, the fluorescent probe could also be used as a probe for the determination of Fe 3 + in cellular and bacterial imaging. Copyright © 2016 John Wiley & Sons, Ltd.
研究领域:
分析化学
·
生物成像
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Zhifeng Cai,Yixuan Zhang,Manlin Zhao,Jinjia Bao,Ling Lv,Haoyang Li
Copper nanoclusters (FA@CuNCs) emitting blue fluorescence were successfully developed via a one-pot technique. In this method, the copper chloride, folic acid and hydrazine hydrate were applied as a precursor, protective agent and reducing agent, respectively. The absorption, fluorescence excitation and emission spectra of FA@CuNCs were carried out by using ultraviolet–visible and fluorescence spectrometry, respectively. The morphology, particle size, functional groups, oxidation states of elements of FA@CuNCs were discussed via using transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS). The stability of FA@CuNCs was studied under various conditions, such as storage time at 25 ℃, ultraviolet radiation time, sodium chloride solutione and pH. The FA@CuNCs displayed blue fluorescence under the excitation wavelength of 361 nm, and the fluorescence quantum yield was 7.45 %. As a result of the inner filter effect, the alizarin red could significantly weaken the blue fluorescence of FA@CuNCs. Thus, the as-prepared FA@CuNCs could be utilized as fluorescence nanosensors for the trace determination of alizarin red. This platform suggested an excellent linear range for alizarin red varying from 0.5 to 200 μM with a fitting coefficient of 0.9955. The detection limit was calculated to be 0.064 μM in the light of the 3b/k (b and k refer to the standard deviation and slope of fitted curve, respectively). Furthermore, the as-developed FA@CuNCs could be used to detect the alizarin red in real samples and for the sensing of temperature.
研究领域:
化学
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材料科学
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分析化学
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 5
·3851-3856
·
IF 3.201
Shuyun Zhu,Cuihua Lei,Yue Gao,Jing Sun,Hongwei Peng,Han Gao,Ruixue Zhang,Rui Wang,Xian-En Zhao,Hua Wang
Ascorbic acid (AA), as one of the most important neurochemicals in cerebral systems, plays a vital role in many physiological and pathological processes. Herein, a facile, label-free, and ultrasensitive fluorescence sensing system was developed for the determination of AA based on the Ag+–o-phenylenediamine (OPD) interaction. OPD could be oxidized by Ag+ to generate fluorescent 2,3-diaminophenazine (OPDox). When AA was introduced, on the one hand, AA can inhibit the oxidation process due to its strong reducing capability. On the other hand, AA can be oxidized to dehydro-AA with an absorption peak at 380 nm, which has a good spectral overlap with the emission of OPDox. Thus, the inner filter effect (IFE) between dehydro-AA and OPDox may occur. Therefore, the introduction of AA can intensively suppress the fluorescence of the Ag+–OPD system. Benefitting from the remarkable synergistic effect of the reducing capability of AA and IFE, a facile and ultrasensitive sensor was constructed successfully for AA sensing. The whole detection procedure was achieved within 10 min. The linear response range of AA was obtained from 0.05 to 40 μM with a detection limit of 10 nM. This developed method has many merits including more simplicity, good selectivity, excellent biocompatibility, and more cost-effectiveness without using any nanomaterials. Notably, the proposed method was successfully applied to detect AA in rat brain microdialysates in the presence of catecholamines, thus providing a new route for AA detection in physiological and pathological fields.
研究领域:
神经化学
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分析化学
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Wendi Han,Chenfang Miao,Xintian Zhang,Yinning Lin,Xiaoli Hao,Zhengjun Huang,Shaohuang Weng,Xinhua Lin,Xianzhong Guo,Jianyong Huang
The content of deferasirox (DEF) in plasma is significant in β-thalassemia patient that needs long-term transfusion therapy, while the effective and simple strategy for DEF monitoring is still limited. The carbon dots (CDs) prepared from citric acid monohydrate and glutathione exhibit weakly modulated fluorescence intensity to several common metal ions containing Cu 2+ . Interestingly, the process of interaction of Cu 2+ and DEF forms the chelation of Cu 2+ and DEF (Cu-DEF) with the absorbance wavelength of DEF at 320 nm shifting to 332 nm for Cu-DEF. And the obtained Cu-DEF will effectively quench CDs through inner filter effect (IFE). Accordingly, a facile signal-off fluorescent method based on CDs as probe is developed for DEF detection using Cu 2+ as medium. And the proposed method exhibits linear range of 0.5–20 μg/mL with the detection limit of 0.33 μg/mL for DEF under the optimized conditions. Moreover, the developed assay is further expanded to test the content of DEF in dispersible tablet and plasma with accuracy and reproducibility. Such cost-effective and sensitive fluorescent assay just through simple mixing operation present a valuable strategy for drug monitoring.
研究领域:
医学
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分析化学
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产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2021
·
IF 4.889
Fanyong Yan,Jingru Sun,Yueyan Zang,Zhonghui Sun,Hao Zhang,Jinxia Xu,Xiang Wang
MXene quantum dots (MQDs) derived from MXene with photoluminescent properties have attracted considerable attention. Herein, nitrogen-doped MQDs (N-MQDs) were constructed by a solvothermal method using Ti 3 C 2 MXene as precursors and o-phenylenediamine (oPD) as the nitrogen source. The morphology, element composition and optical properties of N-MQDs were investigated by transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS) and spectroscopy etc. The obtained N-MQDs are dispersed quasi-spherical nanoparticles , mainly composed of carbon, oxygen and nitrogen, with yellow fluorescence at 580 nm and quantum yield of 5.42 %. In further, N-MQDs can detect Alizarin Red (ARS) based on inner filter effect (IFE) with linear range and detection limit of 0–80 μM and 1.21 μM, respectively. N-MQDs can detect ARS in real water samples, and were expected to have further applications in the fields of environmental monitoring and biosensing.
研究领域:
材料科学
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环境科学
·
分析化学
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产品关联
DOI
高影响因子
JOURNAL OF FLUORESCENCE
·
2019
·Vol. 29
·期 6
·1343-1348
·
IF 1.913
Zheng Chao,Xiang Xu
Several fluorescence spectral features in aggregation of carbazole unites have been reported in previous study. Unfortunately, the influence of inner filter effect has not been paid enough attention. In this work, aggregation of carbazole unites resulting from increasing concentration or decreasing solvent affinity, was investigated by fluorescence spectroscopy. Through comparison between the spectra of two aggregation processes, it is found that only intensity ratio of 0–0 transition to 0–1 transition in excitation/emission spectra is sensitive to aggregation states. There is an obvious decrease in that intensity ratio upon aggregation. On the other hand, other spectral features observed in previous research such as intensity decrease in S 0 → S 2 transition and emission quenching in concentration induced aggregation process, should be induced by inner filter effect.
Graphical Abstract
研究领域:
化学
·
光谱学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 7
·1923-1931
·
IF 4.58
Wu Di,Li Guoliang,Chen Xuefeng,Qiu Nannan,Shi Xuexiang,Chen Guang,Sun Zhiwei,You Jinmao,Wu Yongning
The authors describe a sensitive method for determination of glutathione (GSH) that is based on a thiol-triggered inner filter effect on the fluorescence of N-doped carbon dots (N-doped CDs). N-doped CDs with a quantum yield as high as 31% were prepared by a one-pot procedure, and 5,5′-dithiobis-(2-nitrobenzoic acid) was employed as a reagent for GSH recognition. The reaction product (5-thio-2-nitrobenzoic acid; TNB) acts as an absorber of the 410-nm light used to photo-excite the N-doped CDs. Hence, the fluorescence of N-doped CDs (peaking at 510 nm) is reduced with increasing concentrations of GSH. As little as 30 nM of GSH can be detected by this method. The approach was successfully applied to (a) food analysis, (b) an investigation of an oxidative stress model, and (c) to live cells imaging. The method does not require the surface of N-doped CDs to be chemically modified, and a linkage between receptor and fluorophore is not needed. In our perception, the method may become a viable tool for the detection and imaging of thiols.
Fluorescence sensing strategy for glutathione detection based on a thiol-triggered inner filter effect via new N-doped carbon dots and application to food analysis, oxidative stress study and cell imaging.
研究领域:
分析化学
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生物化学
·
医学检测
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Ruoyu Dai,Yaoping Hu
Quantitative determination of hazardous synthetic colorants in food is quite essential for human health. Herein, a ratiometric fluorescence method is developed for sensitive and selective detection of acid red 18 (AR18) using green/red dual emissive carbon dots (g/r-CDs) as probes. The as-synthesized g/r-CDs exhibit two emission peaks at 508 and 610 nm under excitation of a UV light. When AR18 is added, the green fluorescence of g/r-CDs at 508 nm is quenched dramatically due to the strong inner filter effect; in contrast, the red fluorescence at 610 nm decreases much more mildly. The intensity ratio of I 610 /I 508 has a good linearity with the AR18 concentration over the range of 0.05–8.00 μM. A detection limit of 16 nM is obtained, much lower than the most values reported by former analytical methods. Based on the g/r-CD sensor, accurate determination of AR18 in drinks and candy is successfully realized, demonstrating the good practicability of the proposed method for AR18 monitoring in real samples.
研究领域:
分析化学
·
食品安全
·
材料科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）