MOLECULES
·
2022
·Vol. 27
·期 21
·7636
·
IF 4.927
Xuyan Fan,Tao Tang,Song Du,Ningning Sang,Hao Huang,Chenghui Zhang,Xueping Zhao
A rapid, efficient, simple, and high-throughput method for the simultaneous determination of 108 pesticide residues in three traditional Chinese medicines (TCMs) was established, comprising an improved QuEChERS method in combination with HPLC-MS/MS based on mixed samples. A quantity of 10 mL of acetonitrile was used as extraction solvent, and 10 mg of amino-modified multi-walled carbon nanotubes (MWCNTs-NH2) and 150 mg of anhydrous magnesium sulfate (MgSO4) were selected as sorbents for dispersive solid phase extraction. The performance of the method was verified according to the analytical quality control standards of SANTE/11813/2017 guidelines. With good linearity (R2> 0.9984) in the range of 2–200 μg/L for all pesticides in the selected matrices, and good accuracy, precision, and high sensitivity, the recoveries were in the range of 70–120% for more than 95% of the pesticides, with a relative standard deviation (RSD) of less than 16.82% for all. The limit of detection (LOD) and limit of quantification (LOQ) of the method were 0.01–3.87 μg/kg and 0.07–12.90 μg/kg, respectively, forFritillaria thunbergiiMiq (F. thunbergii),Chrysanthemum MorifoliumRamat (C. morifolium), andDendrobium officinaleKimura et Migo (D. officinale). The method was successfully applied to 60 batches of actual samples from different regions.Keywords:pesticide residue;QuEChERS;MWCNTs-NH2;traditional Chinese medicines
研究领域:
分析化学
·
药学
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DOI
PubMed
高影响因子
Environmental Technology & Innovation
·
2020
·
IF 3.356
Xiaochu Chen,Qizhen Zhou,Fengmao Liu,Qingrong Peng,Yanli Bian
The control and removal of pesticide residues from possible pollution source by using improved methods without secondary pollution is essential for reducing its potential hazards to humans. In this work, a method of combining adsorption and degradation for the removal of pesticide residue was investigated based on the biodegradable microporous starch immobilized laccase (LA–MPS). Biosorbent of microporous starch (MPS) were prepared from maize starch by treating with glucoamylase and α -amylase to increase the specific surface area. Then, laccase with natural biological activity was immobilized on the surface of MPS, the prepared LA-MSP was characterized by SEM, FT-IR, activity assay and stability test. Moreover, LA–MPS coupled with mediators were applied to remove two typical triazine herbicides, atrazine and prometryn, in paddy field water sample. The physical action force between the LA–MPS and pesticide molecules resulted in the good adsorption of LA–MPS to the two pesticides. The apparent rate constants of laccase interaction with pesticide molecules were greatly improved by the adsorption process. Compared with natural degradation, the removal efficiency of atrazine and prometryn were increased by 61% in 7 days, which contributed by 43 ∼ 52% of degradation and 10 ∼ 38% of adsorption. These results indicated that the application of LA–MPS prepared by MPS sorbent was a feasible and green approach for the removal of pesticide residues in paddy field water sample.
研究领域:
环境科学
·
生物技术
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DOI
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 7
·1-7
·
IF 5.705
Liu Qingju,Han Ping,Gong Wenwen,Wang Hui,Feng Xiaoyuan
A method is described for the determination of the pesticide chlorothalonil (CLT). It is based on the finding that citrate-capped gold nanoparticles (AuNPs) undergo aggregation on exposure to chlorothalonil. This is accompanied by a visually detectable color change from wine red to blue. The effect is due to the interaction of the cyano group of chlorothalonil with gold nanoparticles. The assay may also be performed by using a spectrometer. The ratio of absorbances at 700 nm and 520 nm (A 700 /A 520 ) linearly drops in the 5 to 100 ng·mL −1 CLT concentration range, with a 3.6 ng·mL −1 detection limit. This is below the Chinese guideline value for cucumber. The method is rather simple and does not require any modification of the AuNPs or the utilization of antibody. It was successfully applied to the determination of CLT in (spiked) cucumber samples. Recoveries ranged from 80.4 to 97.4%, and the analytical results compared well with those obtained by HPLC.
Schematic of the assay. The strong interaction of the cyano group of acetamiprid with gold nanoparticles (AuNPs) via Au-N bond induces the aggregation of gold nanoparticles, and this is accompanied by a color change from red to purple.
研究领域:
分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Nan Fang,Xueping Zhao,Yanjie Li,Yuqin Luo,Xiangyun Wang,Hongmei He,Changpeng Zhang,Jinhua Jiang
Novel pesticides broflanilide (BFI), afidopyropen (ADP), and flupyradifurone (FPO) have been widely used and become the new organic pollutants . However, uptake, translocation and residual distribution of BFI, ADP, and FPO in plants remain unclear. Therefore, residues distribution, uptake, and translocation of BFI, ADP, and FPO were investigated in mustard field trials and hydroponic experiments. The field results indicated that the residues of BFI, ADP, and FPO were 0.001–1.87 mg/kg at 0–21 d and dissipated fast in mustard (half-lives=5.2–11.3 d). More than 66.5 % of FPO residues were distributed in the cell-soluble fractions because of their high hydrophilicity, while hydrophobic BFI and ADP were primarily stored in the cell walls and organelles . The hydroponic data showed that the foliar uptake rates of BFI, ADP, and FPO were weak (bioconcentration factors<1), but the root uptake rate was strong (bioconcentration factors>1). The upward and downward translations of BFI, ADP, and FPO were limited (translation factor<1). BFI and ADP are uptake by roots via apoplast pathway, and FPO is uptake via symplastic pathway. This study contributes to the understanding of the formation of pesticide residues in plants and provides a reference for safe application and risk assessment of BFI, ADP, and FPO.
研究领域:
植物学
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环境科学
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Ruiqi Liu,Lihong Wang,Tao Lan,Jin Hua,Jingxuan Zhang
In this study, an innovative method for preparing biochar aimed at enhancing the QuEChERS pesticide residue analysis pretreatment technology was developed. The method is ecofriendly and cost-effective. A microporous biochar with a high specific surface area (351.43 m²·g −1 ) was successfully produced using corn stover as the raw material. The preparation process involved sodium hypochlorite delignification, hydrothermal pyrolysis, and slow pyrolysis. This biochar effectively removes pigments and lipids from chrysanthemum extracts, and its three-dimensional structure offers additional adsorption sites for planar compounds and sulfonylurea pesticides. The cost for preparing biochar is approximately 0.5 USD/g, which is significantly lower than that of traditional GCB, offering substantial advantages in both cost and environmental sustainability. The biochar developed in this study not only provides an efficient and economical material for pesticide residue analysis pretreatment but also contributes significantly to the advancement and application of ecofriendly pesticide residue detection technologies.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
CyTA-Journal of Food
·
2025
·
IF 1.7
Zhou Lu,Zhao Junpeng,Hilary Kwesi Ketemepi
A QuEChERS-UPLC-MS/MS method was developed and validated for simultaneous determination of six pesticides (chlorpyrifos, malathion, carbofuran, thiodicarb, deltamethrin, and lambda-cyhalothrin) in dairy products. Samples (n=50), collected from a supermarket in Chongqing, China, were extracted with 1% acetic acid–acetonitrile, purified using PSA, and quantified by external-standard UPLC-MS/MS. All pesticides showed excellent linearity (0.001–0.50 mg/L, R² > 0.99), with LOQs of 0.002 mg/kg. Average recoveries at four spiking levels (0.002, 0.01, 0.1, 1.0 mg/kg) ranged from 89% to 110% (RSD 0.5–9.5%, n=5). Analysis of real samples revealed residues below China’s MRLs (GB 2763–2021), confirming regulatory compliance. The method meets analytical requirements for dairy matrices and offers a reliable framework adaptable to other international standards.
研究领域:
食品科学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 28
·期 13
·16655-16662
·
IF 4.223
Xiao Jinjing,Ma Jinjuan,Wang Fan,Xu Xing,Liao Min,Shi Yanhong,Cao Haiqun
Numerous natural preparations in traditional Chinese medicine are prepared as decoctions. Processing factors (PFs) comparing the levels of pesticide residues in decoctions to those in the corresponding unprocessed products should be considered in exposure assessments. Thus, this study determined the residue levels of six pesticides (chlorpyrifos, phoxim, imidacloprid, thiamethoxam, fenpropathrin, and emamectin benzoate), as well as 3,5,6-trichloropyridinol, the primary metabolite of chlorpyrifos, and clothianidin, the main metabolite of thiamethoxam in Baishao, Paeoniae radix lactiflora (Fam. Ranunculaceae). The results showed that significant time-response effects were present for the release of pesticides from P. radix. The PFs calculated were < 1, indicating a significant reduction in pesticide residues after TCM processing. The water solubility and partition coefficient values of the pesticides may have played a basic role in the dissipation of the residues during the TCM decocting process. A risk assessment based on the hazard quotient with PFs revealed that exposure to pesticide residues in P. radix was far below the levels that might pose a health risk. In conclusion, the results presented here are of theoretical and practical value for the safety evaluation of TCMs.
研究领域:
药理学
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毒理学
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中医药学
✓ 已验证
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Ying Wang,Siqi Li,Jing Sun,Yufang Li,Yan Hu,Chuanpu Shen,Feilong Liu,Shiwen Guo,Feiran Chen,Qiangjian Liu,Jingjing Ma,Fucai Ren,Furong Yang,Qunlin Zhang,Jingyuan Song,Tianyi Xin,Xiangdong Pu
Adulteration, pesticide residues, and fungal contamination are the three key factors affecting herbal medicine safety. Herbal tea is a healthy beverage containing bioactive molecules and is one of the derivatives of medicine food homology herbal medicine. However, these three factors are often ignored in the herbal tea industry. In this study, 171 commercially available Panax herbal tea products were tested. The findings revealed that the target Panax ingredient in the herbal tea products was adulterated by Platycodon grandiflorum or the mixed-using of Panax genus species, with an adulteration rate of 25.1%. The pentachloronitrobenzene (PCNB) pesticide residue rate was 78.4% in the 171 Panax herbal tea products. Two samples demonstrated obvious fungal contamination. Among the 171 Panax herbal teas, 98 samples were qualified with the correct species, qualified pesticide residues, and no fungal contamination (eyeballing). The present investigation not only sheds light on the three key factors exerting a significant impact on herbal tea safety but also provides a reference for the comprehensive evaluation of herbal tea, thereby facilitating the development of this industry.
研究领域:
中药学
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食品安全
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植物化学
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产品关联
DOI
高影响因子
MOLECULES
·
2017
·Vol. 22
·期 4
·560
·
IF 2.861
Yating Zhang,Lingbiao Gu,Fei Wang,Lingjun Kong,Guangyong Qin
As a natural and healthy beverage, tea is widely enjoyed; however, the pesticide residues in tea leaves affect the quality and food safety. To develop a highly selective and efficient method for the facile removal of pesticide residues, the subcritical butane extraction (SBE) technique was employed, and three variables involving temperature, time and extraction cycles were studied. The optimum SBE conditions were found to be as follows: extraction temperature 45 °C, extraction time 30 min, number of extraction cycles 1, and in such a condition that the extraction efficiency reached as high as 92%. Further, the catechins, theanine, caffeine and aroma components, which determine the quality of the tea, fluctuated after SBE treatment. Compared with the uncrushed leaves, pesticide residues can more easily be removed from crushed leaves, and the practical extraction efficiency was 97%. These results indicate that SBE is a useful method to efficiently remove the bifenthrin, and as appearance is not relevant in the production process, tea leaves should first be crushed and then extracted in order that residual pesticides are thoroughly removed.Keywords:subcritical butane;pesticide residue;bifenthrin;black tea
研究领域:
食品科学
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环境科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD PROCESS ENGINEERING
·
2023
·Vol. 46
·期 12
·e14458
·
IF 3
Shaofeng Yuan,Hang Yu,Yahui Guo,Yunfei Xie,Yuliang Cheng,He Qian,Weirong Yao
The effect of ultrasound, ozone, and their combination treatment on the removal of two common pesticides (trichlorfon and phoxim) in pak choi samples was studied in this work. The results showed that both ultrasound and ozone treatment alone can significantly remove trichlorfon and phoxim residues in pak choi (>90%), but they reduced the quality of pak choi (such as vitamin C and color). Ultrasound and ozone had a synergistic effect on the removal of pesticide residues in pak choi. The synergistic effect of combined treatment was evaluated from the perspective of pesticide removal speed, and found that their combination significantly accelerated the removal of pesticides. Compared with ultrasound or ozone alone, the removal rate constant of trichlorfon by the combined treatment increased by 121% and 347%, and that of phoxim increased by 159% and 367%, respectively. In addition, the combined treatment of ultrasound and ozone can well maintain the vitamin C and color of pak choi while removing pesticides. Therefore, the combined treatment of ultrasound and ozone can be used as an effective cleaning method to remove pesticide residues in vegetables. Practical applications Reducing pesticide residues in vegetables is of great significance to human health, and how to maintain the quality of vegetables as much as possible while removing pesticides is a key issue. In this study, the combination of ultrasound and ozone significantly accelerated the removal of pesticides, and retained the vitamin C and color of pak choi well. Therefore, the combined application of ultrasound and ozone has the potential to be an industrial cleaning method for removing pesticide residues in fruits and vegetables without compromising quality.
研究领域:
食品科学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Yupeng Wen,Wendi Huo,Xinyuan Zhang,Yingchun Mu,Huan Liu,Chaoying Zhang,Lu Qiao,Jincheng Li
In this study, we developed a miniaturized semi-automatic purification method based on pressure-filtration purification for sensitive HPLC-MS/MS monitoring of pesticides in aquatic products. For the first time, loofah sponge was demonstrated and utilized as a novel green, low-cost, and effective one-step purification adsorbent for fish sample. The developed method consisted of two main steps: first, target analytes were extracted from fish samples using an acetonitrile-based QuEChERS extraction method. Subsequently, the extract was automatically purified through a pressure-filtration purification column in a centrifuge, followed by HPLC-MS/MS analysis. Under optimized experimental conditions, the method exhibited excellent linearity, with a range of 1.0 to 50.0 μg/kg and a correlation coefficient > 0.99. The detection and quantification limits were 0.5 and 1–2 μg/kg, respectively. Recoveries at spiked levels of 2.0, 5.0 and 30.0 μg/kg ranged from 69.95 to 104.88 %, with intra-day RSDs ranging from 0.58 to 15.01 % and inter-day RSDs ranging from 0.72 % to 14.32 %.
研究领域:
食品科学
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环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Mantang Chen,Linping Shi,Fuwei Xie,Xiaoyu Wang,Cong Nie,Sheng Wang,Kejian Liu,Jingjing Yu,Libin Wan
A flat membrane–based liquid-phase microextraction (FM-LPME) method was developed for the first time to simultaneously separate and extract basic and acidic pesticide analytes from the same sample. Using carbendazim and pirimicarb as representative basic pesticides, along with bromacil, diflubenzuron and 1,2-dibenzoyl-1-tert-butylhydrazine (RH-5849) as representative acidic pesticides, the performance of FM-LPME for the extraction of these model pesticides has been optimised individually. Under optimal extraction conditions, the developed FM-LPME-LC–MS/MS method exhibited excellent linearity, with R 2 values exceeding 0.9903 in concentration ranges of 0.1–1400 ng mL −1 for carbendazim and pirimicarb, and 1–1400 ng mL −1 for bromacil, diflubenzuron and RH-5849. The limits of detection ranged from 0.01 to 0.09 ng mL −1 . The recoveries and relative standard deviations of all the tested pesticides at three spiked concentrations (100, 500 and 1200 ng mL −1 ) were in the ranges of 80.0%–125.0% and 0.3%–9.3%, respectively. The FM-LPME-LC–MS/MS method demonstrated advantages in terms of being environmentally friendly, possessing a wide linear range, high sensitivity, high precision and satisfactory recoveries, thus highlighting its notable potential for the separation and extraction of basic and acidic pesticide residues in water samples.
研究领域:
分析化学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）