Scientific Reports
·
2025
·Vol. 15
·期 1
·1-16
·
IF 3.9
Wang Qing,Chen Jie-Jie,Qiu Bin,Zhou Liang,Liu Rui-Ye,Huang Jia-Fen,He Yan,Xie Qin
Taking the extraction amount of rutin as the index, the extraction process of rutin from Ilex asprella (Hook. et Arn.) Champ. ex Benth. using the ultrasonic-assisted deep eutectic solvent method was optimized through response surface method. The effects of various factors, including the types of deep eutectic solvent, molar ratio of the solvent, water content, liquid-solid ratio, the temperature, power and time of ultrasound on the extraction amount of rutin were investigated. In accordance with the result of univariate experiments, the extraction process was optimized through employing the Box-Behnken response surface design method. A three-factor, three-level experimental model was established with the liquid-solid ratio, water content and ultrasonic time as variables. The findings indicated that the optimal technological conditions were as stated below: the molar ratio of lactic acid to choline chloride at 1:1, the ultrasonic extraction temperature at 40 °C, the ultrasonic extraction time at 31 min, the water content at 28%, and the liquid-solid ratio at 20:1 mL/g. Under optimal conditions, the validation experimental result revealed that the extraction amount of rutin was 86.553 ± 1.35 µg/g, with an absolute error of less than 0.6% in comparison with the predicted value of the model. The use of deep eutectic solvents for the extraction of rutin and the application of response surface method for optimizing the extraction process of rutin from Ilex asprella (Hook. et Arn.) Champ. ex Benth. were demonstrated. The present study provided a reference for the comprehensive utilization of Ilex asprella (Hook. et Arn.) Champ. ex Benth. .
研究领域:
植物化学
·
天然产物开发
·
药用植物研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION SCIENCE AND TECHNOLOGY
·
2021
·
IF 2.475
Weiyue Wang,Zenghao Yan,Hongliang Yao,Peibo Li,Wei Peng,Weiwei Su,Yonggang Wang
An efficient and low-cost method was developed for the preparation of pedunculoside from the dried barks of Ilex rotunda using crystallization combined with polyamide column chromatography. The kinetics of ethanol extraction was first evaluated and then the extract was concentrated to remove ethanol. Pedunculoside was partially crystallized after ethanol removal and those left in solution were further separated by polyamide column chromatography. To optimize the operating parameters for chromatography, the static and dynamic adsorption/desorption experiments were conducted. The purity of pedunculoside in the product increased to 85.69%, with an overall yield of 66.71%. The extraction and purification method developed herein provides a reference standard and can be used as the starting material for further studies.
研究领域:
植物化学
·
分离科学
·
天然产物化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
·
2021
·
IF 1.571
Fang Chen,Shi-Yun Huang,Shu-Qing Xiang,Xiao-Xu Qiao,Guo-Chao Liao,Zhong-Qiu Liu,Hua Zhou,Peng Wu
Two new phenolic glycosides (1-2), along with six existing compounds (3-8), were isolated from the ethanolic extract of Ilex pubescens roots, a traditional folk medicine. These structures were determined using HR-ESI-MS, IR, UV, and NMR (including 1 D, 2 D-NMR). The anti-inflammatory activities of three phenolic glycosides (1-3) were evaluated in the human HepG2 cell lines. The results showed that compound 3 could induce P-gp and BCRP expression through the Nrf2-mediated pathway.
研究领域:
天然产物化学
·
药理学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Food & Function
·
2017
·Vol. 8
·期 10
·3688-3695
·
IF 3.247
Xueli Cao,Yu Liu,Jing Li,Lan Xiang,Hiroyuki Osada,Jianhua Qi
Ilex latifolia Thunb is a traditional Chinese tea and herbal medicine. In this study, one new triterpene saponin (1) and six known triterpenoids (2–7) were isolated from the methanol extract of I. latifolia using a PC12 cell bioassay system. The structures and stereochemistry of these compounds were elucidated using spectroscopic methods and chemical derivatization. This new triterpene saponin (1) was characterized as an ursolic type acid with a 19α-hydroxyl and a trisaccharide moiety at C-3. Compound 1 significantly promoted the neurite outgrowth in PC12 cells by 52% at 10 μM, whereas compounds 2–7 showed less neuritogenic activity. Structure activity relationship studies indicated that introducing a trisaccharide moiety at C-3 is important for the neuritogenic activity, but the sugar group at C-28 decreased this activity. In addition, compound 1 increased the neurite outgrowth length in primary cortical neuron cells of mice and also exhibited a neuronal protection effect on H2O2-damaged PC12 cells at optimum concentrations.
研究领域:
药理学
·
植物化学
·
神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Jie Yu,Zhonghang Chen,Jiefan Zhu,Viachaslau Zhylko,Liyan Dai
The aqueous two-phase extraction process (APTE) was utilized for the extraction of polysaccharides from the fruits of Ilex cornuta (Lindl. et Paxt.) (PFIC). After screening and selection of isopropanol/ammonium sulfate as the aqueous two-phase system, various parameters of crude extract amount, ultrasonic time, extraction temperature and extraction time on the extraction process was investigated in detail and optimized by response surface methodology (RSM). Under the optimal conditions in which crude extract amount was 2.23 g, ultrasonic time was 17.5 min, extraction temperature was 69 °C and extraction time was 1.55 h, the yield of polysaccharides reached 50.87% which was close to the predicted value of 50.78%. Structural features of PFIC were investigated by GPC, GC–MS and FT-IR analysis. And the results indicated that PFIC with a molecular weight of 3.89 kDa was composed of monosaccharides including rhamnose (1.83%), arabinose (11.70%), xylose (3.30%), mannose (3.55%), glucose (61.88%) and galactose (17.74%). The bioactivity experiments showed that PFIC had strong scavenging ability on DPPH radical, fine scavenging ability on hydroxyl radical and certain scavenging ability on superoxide anion radical. Overall, these results suggested that PFIC exhibiting fine to good antioxidant capacities could be potentially developed as a natural antioxidant.
研究领域:
生物化学
·
药学
·
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
MycoKeys
·
2022
·
IF 3.111
Zhaoxue Zhang,Rongyu Liu,Shubin Liu,Taichang Mu,Xiuguo Zhang,Jiwen Xia
Species of Sporocadaceae have often been reported as plant pathogens, endophytes or saprophytes and are commonly isolated from a wide range of plant hosts. The isolated fungi were studied through a complete examination, based on multilocus phylogenies from combined datasets of ITS/ tub2 / tef1 , in conjunction with morphological characteristics. Nine strains were isolated from Ficus microcarpa , Ilex chinensis and Schima superba in China which represented four species, viz. , Monochaetia schimae sp. nov. , Neopestalotiopsis haikouensis sp. nov. , Neopestalotiopsis piceana and Pestalotiopsis licualicola . Neopestalotiopsis piceana was a new country record for China and first host record from Ficus macrocarpa . Pestalotiopsis licualicola was first report from Ilex chinensis in China.
研究领域:
真菌学
·
植物病理学
·
分类学
·
菌物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Arabian Journal of Chemistry
·
2024
·
IF 5.3
Hui Liu,Xiao Hu,Mingyi Hu,Xinxin Liu,Jiangping Huang,Zhixuan Ying,Shuiqing Chen,Lingguang Yang,Peipei Yin
Kuding tea (KT) is the general name of a set of bitter-tasting herbal tea made from varied original plant species. However, limited information was reported about the difference of bioactive constituents, heavy metals, and bioactivities among those original species. In the present study, comparison analyses among heavy metals, polysaccharides, phenolics, flavonoids, and saponins constituents, as well as the antioxidant and antidiabetic activities were performed through colorimetry, UPLC-QTOF-MS, enzymatic assay and molecular docking techniques on the six KT original plants mainly from the genus Ilex . Results showed that Cd contents in the four KT original plants other than Ilex pubescens (IP) and Ilex cornuta (IC) and heavy metal contents including Pb, Cu, As and Hg in the all six plants were lower than standard limit values. Furthermore, contents of carbohydrate in polysaccharides (470.06 and 548.51 mg GLE/g), total phenolics (33.84 and 48.85 mg GAE/g), flavonoids (30.86 and 109.97 mg RE/g), phenolic acids (49.68 and 61.48 mg CAE/g), flavonols (18.47 and 19.19 mg RE/g), flavanols (0.18 and 0.14 mg CE/g), saponins (90.81 and 97.33 mg GSE /g) in IP and Ligustrum japonicum (LJ), as well as their antioxidant activity levels (IC 50 607.41–780.44, and 547.06–754.00 mg/L) were all significantly higher than those in other four KT original plants. Moreover, a total of 6 and 9 compounds were tentatively characterized from IP and LJ, respectively, and molecular docking and molecular dynamics simulation were further used to analysis the interaction between α-glucosidase and bioactive compounds in IP. At last, principal component, hierarchical cluster, and correlation analysis indicated that carbohydrate content of polysaccharides, total phenols, flavonoids and phenolic acids were the main influencing factors to antioxidant abilities. Overall, according to our systematic analysis, IP possessed the highest antioxidant and antidiabetic potentials, thus might be a better KT original plants from the genus Ilex for further development and utilization.
研究领域:
药用植物学
·
分析化学
·
植物科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 13
·3999
·
IF 4.927
Ying Chen,Xuqiang Sun,Lanting Fang,Xinxiu Jiang,Xuena Zhang,Zijun Ge,Rongbin Wang,Cunqin Wang
The polyphenolic extract ofIlex latifolia(PEIL) exhibits a variety of biological activities. An evaluation of the parameters influencing the ultrasonic extraction process and the assessment of PEIL antioxidant activity are presented herein. Response surface methodology (RSM) was used to optimize the experimental conditions for the polyphenols ultrasonic-assisted extraction (UAE) from the leaves ofIlex latifolia. We identified the following optimal conditions of PEIL: ethanol concentration of 53%, extraction temperature of 60 °C, extraction time of 26 min and liquid–solid ratio of 60 mL/g. Using these parameters, the UAE had a yield of 35.77 ± 0.26 mg GAE/g, similar to the value we predicted using RSM (35.864 mg GAE/g). The antioxidant activity of PEIL was assessed in vitro, using various assays, as well as in vivo. We tested the effects of various doses of PEIL on D-galactose induced aging. Vitamin C (Vc) was used as positive control. After 21 days of administration, we measured superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities, malondialdehyde (MDA) levels in mouse serum and liver tissue. The results demonstrated that the PEIL exhibits potent radical scavenging activity against 1,1-diphenyl-2-picrythydrazyl (DPPH∙), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS+), and hydroxyl (∙OH) radicals. The serum concentrations of SOD and GSH-Px were higher, and MDA levels were lower, in the medium- and high-dose PEIL-treated groups than those in the aging group (p< 0.01), and the activity of MDA was lower than those of the model group (p< 0.01). The liver concentrations of SOD and GSH-Px were higher (p< 0.05), and MDA levels were lower, in the medium- and high-dose PEIL-treated groups than those in the aging control group (p< 0.01). These results suggest that optimizing the conditions of UAE using RSM could significantly increase the yield of PEIL extraction. PEIL possesses strong antioxidant activity and use as a medicine or functional food could be further investigated.Keywords:Ilex latifolia;polyphenols;response surface methodology;antioxidant activity
研究领域:
药理学
·
植物学
·
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）