Antioxidants
·
2022
·Vol. 11
·期 12
·2406
·
IF 7.675
Xiang-Rong Cheng,Bu-Tao Yu,Jie Song,Jia-Hui Ma,Yu-Yao Chen,Chen-Xi Zhang,Piao-Han Tu,Mitchell N. Muskat,Ze-Gang Zhu
Food-derived electrophilic compounds (FECs) are small molecules with electrophilic groups with potential cytoprotective effects. This study investigated the differential effects of six prevalent FECs on colitis in dextran sodium sulfate (DSS)-induced mice and the underlying relationship with molecular characteristics. Fumaric acid (FMA), isoliquiritigenin (ISO), cinnamaldehyde (CA), ferulic acid (FA), sulforaphane (SFN), and chlorogenic acid (CGA) exhibited varying improvements in colitis on clinical signs, colonic histopathology, inflammatory and oxidative indicators, and Nrf2 pathway in a sequence of SFN, ISO > FA, CA > FMA, CGA. Representative molecular characteristics of the “penetration-affinity–covalent binding” procedure, logPvalue, Keap1 affinity energy, and electrophilic index of FECs were theoretically calculated, among which logPvalue revealed a strong correlation with colitis improvements, which was related to the expression of Nrf2 and its downstream proteins. Above all, SFN and ISO possessed high logPvalues and effectively improving DSS-induced colitis by activating the Keap1–Nrf2 pathway to alleviate oxidative stress and inflammatory responses.Keywords:electrophilic compound;colitis;molecular characteristic;logP
研究领域:
药理学
·
免疫学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2019
·Vol. 43
·期 4
·839-846
·
IF 2.516
Guo Yu,Xing Fan,Xue-Ming Dong,Rui-Yu Wang,Yun-Peng Zhao,Hong-Cun Bai,Tian-Sheng Zhao,Qing-Jie Guo,Xian-Yong Wei
Dayan lignite was subjected to thermal dissolution sequentially with cyclohexane, acetone, and methanol. Each thermal dissolution extract was subjected to further separation/enrichment using column chromatography, which was sequentially eluted with petroleum ether, a mixture of ethyl acetate and petroleum ether (vol:vol = 1:1), and ethyl acetate. The three thermal dissolution extracts and nine enrichment subfractions were characterized by an Orbitrap mass spectrometry equipped with an atmospheric pressure chemical ionization ion source. The mass spectrometry data were also statistically analyzed by principal component analysis, which can reduce the dimensionality of data and classify multiple samples according to principal components. Identified compounds in the extracts and subfractions are classified into eight classes according to the heteroatom distribution. Hydrocarbon class is mainly presented in the petroleum ether fraction, and oxygen class, nitrogen class, and oxygen-nitrogen class are distributed in both petroleum ether/ethyl acetate and ethyl acetate subfractions. The combination of different analytical methods enhances the understanding of coal at the molecular level and provides important data for downstream refining processes.
研究领域:
化学
·
能源科学
✓ 已验证
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2018
·
IF 5.089
Xiao-Dan Shi,Jun-Yi Yin,Liu-Jing Zhang,Ou-Ye Li,Xiao-Jun Huang,Shao-Ping Nie
Aloe barbadensis Miller ( Aloe vera ) has long been used in food , cosmetic and pharmaceutical industries. The solid wastes such as the spikes and the outer green skins are usually separated from the inner gel and discarded during the process of Aloe vera leaves. Three kinds of polysaccharides (designated as ASP-4N, ASP-6N and ASP-8N) were successfully purified from the leaf skin of Aloe vera in our previous work. The present study aimed to analyze the structural and molecular features of ASP-6N and ASP-8N using methylation and GC-MS, NMR, high performance size-exclusion chromatography coupled with multi-angle laser light scattering (HPSEC-MALLS) and scanning electron microscopy (SEM) techniques. Results showed that ASP-6N and ASP-8N were β-(1→4)-glucomannans with acetyl groups, which may attach to O -2, O -3 or O -6 of mannopyranosyl residues in the backbone as mono-, di-, or tri-acetylated forms. HPSEC-MALLS analysis indicated that the polysaccharide fractions from Aloe leaf skin adopted various conformations in the aqueous solution depending on their molecular mass. The polysaccharides had fibrous, ribbon-like and spherical morphology by SEM observation.
研究领域:
植物化学
·
食品科学
·
药学
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2022
·
IF 6.449
Yan-Chan Wei,Ding Zhu,Wen-Yi Xie,Jie-Hui Xia,Meng-Fan He,Shuangquan Liao
Natural rubber (NR), an important source of elastomeric material, has superior comprehensive properties compared to synthetic high cis -1, 4-polyisoprene, which are attributed to its peculiar architecture. However, it is a challenge to identify the precise structure of NR, especially the structure of NR particles, as there are no direct characterization methods to perform in-situ investigation of structure of NR particles at an intact state. In this study, we used cryogenic transmission electron microscopy (cryo-TEM) to directly explore the morphology of NR particles existing in the latex without drying or chemical fixation. Then, the spatial organization of proteins and phospholipids on the surface of NR particles was visualized by stochastic optical reconstruction microscopy (STORM). The results show that NR particles are spherical particles with an average particle size of 88 ± 15 nm, and the exterior of NR particles is a mixed monolayer of proteins and phospholipids (ca. 4.6 ± 0.77 nm in thickness). Thus, a more complete model for the Hevea brasiliensis rubber particles was proposed. Moreover, we explicated the relationship between the particle size of NR particles, molecular characteristics, and mechanical properties of NR. The M n and M w of small rubber particles (SRPs) with a particle size of 106 nm are (1.56 ± 0.06) × 10 6 g/mol and (2.21 ± 0.07) × 10 6 g/mol respectively, which are much larger than those of the large rubber particles (LRPs) with a particle size of 628 nm ( M n : (0.41 ± 0.05) × 10 6 g/mol; M w : (1.63 ± 0.10) × 10 6 g/mol). After vulcanization, the SRPs have better mechanical properties. Compared with LRPs, SRPs exhibit a 4.3-fold increase in tensile strength (from 5.7 to 24.6 MPa) and a 3.5-fold increase in toughness (from 12.9 to 44.9 kJ/m 2 ). This work not only provides rational design for the new biomimetic synthetic rubber with unique hierarchical structures and excellent properties, but also offers a new possibility for tuning the mechanical properties of NR by preparation of LRPs/SRPs blend of various ratios.
研究领域:
材料科学
·
生物学
✓ 已验证
产品关联
DOI
高影响因子
WATER RESEARCH
·
2022
·
IF 13.4
Junjie Yu,Hui Xu,Dongsheng Wang,Hongyan Sun,Ruyuan Jiao,Yang Liu,Zhiyuan Jin,Shuo Zhang
Most studies on the interaction between coagulation and NOM (natural organic matter) currently focus on pollutant removal and coagulant species distribution, while studies on floc aging are lacking. Investigation onto the effects of floc aging could guide further processes that utilize flocs, such as densadeg sludge recirculation, floc predeposition for ultrafiltration, sludge condensation, and other traditional sludge reflux processes. In this study, flocs generated by Al 13 and AlCl 3 in microparticle- and nanoparticle-containing water were investigated, and the effect of floc aging on NOM was quantified based on several organic matter characterization techniques. Flocs absorb and release organics during aging. The flocs generated from micro-SiO 2 have a significant absorbing effect for LWM-N (low-molecular-weight neutral substances) and protein-like substances, while the absorption of NOM by flocs generated from nano-SiO 2 is insignificant. HS (humic substances) with high aromaticity are released during floc aging. From the molecular perspective, the molecules released during floc aging are those with higher double bond equivalents and higher aromaticity, while the absorbed molecules are those with lower double bond equivalents and lower aromaticity. 2D-COS (two-dimensional correlation spectroscopy) demonstrated that the flocs generated by Al 13 and AlCl 3 had the same organic release patterns but different intensities, while the flocs generated in the micro-SiO 2 and nano-SiO 2 systems had different organics release patterns. Abundant aluminum hydrolysates with low polymerization and amorphous Al(OH) 3 would be produced from AlCl 3 during the coagulation process and then undergo hydroxyl‑bridging reaction and crystallization during floc aging, thus releasing more HS with high aromaticity into the supernatant; in comparison, prehydrolyzed Al 13 produces a more stable floc and releases less HS during aging. The flocs produced by nano-SiO 2 and Al-based coagulants release higher aromaticity HS into the water than those produced by micro-SiO 2 , which may be related to the formation of more highly polymerized degree hydrolysates and nanocrystalline Al(OH) 3 in the nano-SiO 2 system. The flocs generated in water with micro-SiO 2 may contain a large amount of Al-OH and have a loose structure, thus further absorbing NOM, such as protein-like substances and LWM-N. In contrast, the flocs generated from nano-SiO 2 possess abundant adsorbed water and a denser structure; thus, organic matter cannot be absorbed stably.
研究领域:
环境科学
·
水处理工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Weiping Jin,Yiran Yu,Wenfu Hou,Guozhen Wang,Zhenzhou Zhu,Jingren He,Shuiyuan Cheng,Qingrong Huang
Selenium is an essential trace element in human body, and kappa-selenocarrageenan (Se-car) is an organic source of selenium supplement. To further utilize Se-car in food packaging, biotherapy or biosensor, the molecular information of Se-car was characterized here and multi-functional Ag NPs synthesized by Se-car were fabricated. Results of GPC-MALLS, FTIR , potentiometric titration, and intrinsic viscosity showed that Se-car was polymerized by nearly 22 basic units of disaccharide . Sixty-four percentage of sulfated groups (SO 4 2− ) in carrageenan was replaced by selenium acid (SeO 3 2− ), which belonged to weak acid resulting from a gradually decrease of ζ-potential with acidity process to pH 1.0. Besides, the capacity of biosynthesis silver nanoparticles (Ag NPs) by Se-car was studied and it made a comparison with κ-carrageenan. Results exhibited that Se-car could serve as an efficient reducing and capping agent for Ag NPs fabrication (remarked as Se-car@Ag). The k app of Se-car@Ag NPs for catalyzing 4-NP degradation was 2.14 × 10 −2 s −1 . Antibacterial test revealed Se-car@Ag had an ability to inhibit the growth of Escherichia coli and Staphylococcus aureus . To combine the selenium health benefit and functional metal nanoparticles, Se-car@Ag might have potential applications in multiple areas like medicine, disease diagnostic, and drug delivery.
研究领域:
食品科学
·
材料科学
·
生物医学
✓ 已验证
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Wenjia Yan,Minghao Zhang,Meng Zhang,Madhav P. Yadav,Xin Jia,Lijun Yin
The effect of wheat bran arabinoxylan (WBAX) with different molecular characteristics on the gelatinization and long-term retrogradation behavior of wheat starch (WS) have been evaluated. WBAXs with M w of 280–754 kDa and Ara/Xyl of 0.45–0.62 were obtained through alkaline extraction with graded ethanol precipitation . WBAXs with larger M w and branching degree could impede gelatinization process of wheat starch by effectively decreasing the water availability for starch gelatinization. The hydrogen-bonding interactions between WS and WBAX could enhance the gel strength of WS-WBAX mixed pastes. WBAXs with lower branching degree could inhibit the long-term retrogradation of starch through hindering the rearrangement of amylopectin and double-helical associations of amylose during long-term storage, due to hydrogen-bonding interaction between WBAX and starch. Low-field nuclear magnetic resonance (LF-NMR) relaxometry analysis confirmed that the addition of WBAXs could improve the water holding properties of retrograded starch gels.
研究领域:
食品科学
·
植物化学
✓ 已验证
DOI
PubMed
高影响因子
Advanced Fiber Materials
·
2022
·Vol. 4
·期 6
·1645-1655
·
IF 12.958
Wang Chunyan,Hou Yaqi,Wang Xingyi,Li Weijun,Zhang Yunmao,Wang Shuli,Zheng Jing,Hou Xu
Catheterization is indispensable in the field of modern medicine. However, catheter-related thrombosis and infections almost inevitably occur during the process, and as drugs can only be administered at the end of catheter, auxiliary strategies are required for successful implantation. Considering these intractable limitations, a type of self-adaptive, anti-coagulate liquid-based fibrous catheter has been developed. More importantly, it has positional drug release property that traditional catheters desperately need but couldn’t attain. Although enlightening, the feasibility and performance of the positional drug release have only been demonstrated by fluorescents, the specific drug release kinetics remains unknown for adaptation to application scenarios. Therefore, we systematically investigate the structural and interfacial effects of drug molecules and fibrous matrixes on drug release kinetics in a liquid-based fibrous catheter. Theoretical calculations and experiments demonstrate that oleophilic and hydrophilic molecules release slowly due to a dissolution-diffusion mechanism. Amphipathic molecules, however, will significantly affect the gating performance by affecting the interfacial stability, hence they release quickly with emulsifying the gating liquid. Besides the significant impact of molecular properties and interfacial effects, matrix pore size also has a slight influence that molecules release faster in bigger pores. Through this study, the liquid-based fibrous catheter may step further toward practical applications including chemotherapy, haemodialysis, angiography, etc . to overcome the existing catheter-related limitations. Graphical Abstract
研究领域:
生物医学工程
·
药剂学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Tong Shi,Hui Liu,Teng Song,Zhiyu Xiong,Li Yuan,David Julian McClements,Wengang Jin,Quancai Sun,Ruichang Gao
The effects of l -arginine (Arg)-assisted ultrasonic treatment on the molecular and interfacial characteristics of myosin and emulsifying properties of the emulsion were evaluated to ascertain the underlying mechanism in improving the emulsion stability. Ultrasonication induced the exposure of residues of native myosin, which was increased by the addition of Arg (40 mM). Furthermore, in terms of emulsions containing Arg, the higher the ultrasonication intensity was, the greater the increase in adsorbed protein (from 15.43 ± 0.28% to 50.49 ± 1.65%) and π value, and the decrease in droplet sizes (from 4098 nm to 2324 nm) ( P < 0.05). Moreover, the increase in the ordered structures of interfacial myosin induced by Arg and ultrasonication favoured the formation of a protein gelation network. In summary, Arg-assisted ultrasonic treatment improved the stability of the emulsion by inducing the exposure of native myosin and facilitating the formation of ordered structures of interfacial myosin.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2020
·
IF 7.053
Jinxin Yan,Boya Zhang,Feifei Wu,Wenjia Yan,Peng Lv,Madhav Yadav,Xin Jia,Lijun Yin
This study investigated the molecular characteristics and rheological properties of arabinoxylans (AX) extracted from three different red sorghum brans from Shandong, Shanxi and Hebei Province in China. It was found that only the Shandong sorghum bran AX, with its high ferulic acid content and low Ara/Xyl ratio, could form a gel induced via peroxidase. Subsequently, this AX and soy protein isolate (SPI) were used to prepare the mixed gels using two crosslinking agents, namely peroxidase and calcium ions , which induced AX and SPI to form the gel networks, respectively. Dynamic rheological tests showed that the doubly induced AX-SPI mixed gel had higher storage modulus (G′) and loss modulus (G″) than mixed gel by only peroxidase or AX gel induced by one or two crosslinking agents. Moreover, Texture Profile Analysis (TPA) confirmed that the doubly induced mixed gel was harder and more elastic than the other gels. Fourier-Transform Infrared Spectroscopy (FT-IR) further confirmed that, in addition to the presence of AX intermolecular covalent cross-linked gel network by peroxidase and the connection of SPI intermolecular gel network via salt bridge (Ca 2+ ), a partial covalent crosslinking network between AX and SPI existed in the doubly induced mixed gel. Microstructure observation of the gels revealed that the doubly induced AX-SPI mixed gel had a more regular, uniform and dense inner structure than the AX single network gel. Gels with different mechanical properties and network structures could be prepared by adjusting the order of addition of the crosslinking agents.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
CATALYSIS TODAY
·
2021
·
IF 6.766
Jiyuan Fan,Saumitra Saxena,Chengkun Xiao,Jinlin Mei,Gang Wang,Aiping Chen,Wen Zhang,Haidong Li,Aijun Duan,William L. Roberts
Oxidative desulfurization of model oil and heavy fuel oil (HFO) was investigated under mild conditions using H 2 O 2 as an oxidant and acetonitrile as an extractant. The influence factors in the oxidation and extraction processes were evaluated and optimized. The molecular characteristics of the raw feedstock and the desulfurized oils, and the corresponding extraction phases were systematically analyzed by APPI FT-ICR MS, 1 H NMR, etc. The results showed that the desulfurization rate of dibenzothiophene reached 97.7% after 10 min reaction, while the sulfur removal efficiency of HFO was 30.7% under optimized conditions. The structures of sulfur compounds were described in heteroatom type, carbon number distribution vs unsaturation degree. The detected main sulfur-containing compounds are S 1 , S 2 , S 3 , NS, OS, O 2 S, O 2 S 2 , and O 4 S 2 . Based on the APPI FT-ICR MS results, it can be found that almost all the S 1 species have been transformed into sulfone after the oxidation process. Furthermore, the sulfone of O 2 S 1 and O 4 S 2 species in extraction phases were in double bond equivalent (DBE) range from 9 to 25 and carbon number with ≤ 40. 1 H NMR results showed that the α to aromatic CH 3 combined naphthenic CH-CH 2 group accounted for 73.2% in the extraction phase.
研究领域:
化学工程
·
分析化学
·
环境科学
✓ 已验证
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2024
·
IF 18.5
Xin-Mei Hu,Yang Shen,Shi-Chi Feng,Zhen-Huang Su,Kai Zhang,Long-Xue Cao,Bing-Feng Wang,Xingyu Gao,Jian-Xin Tang,Yan-Qing Li
Perovskite light-emitting diodes (PeLEDs) have garnered extraordinary attention in displaying field owing to their excellent luminescence properties. Although exogenous additives are extensively employed for optimizing PeLEDs, their comprehensive regulation including side effects still lacks in-depth study. Here for the first time, it is demonstrated that the deprotonation degree of additives significantly influences the performance of blue PeLEDs. Benzenesulfonic acid (BSA) and ammonium benzenesulfonate (ABS) with similar molecular structures while distinctly different acid dissociation constants (p K a ) are used for modifying blue perovskites. By comparison, high-p K a ABS holds greater potential in boosting device performance, contributing to an improved peak external quantum efficiency of 18.8%. This discrepancy is ascribed to the fact that low-p K a BSA is prone to induce prominent perovskite chlorine loss owing to its intense deprotonation, while high-p K a ABS significantly suppresses chlorine vacancy formation. Meanwhile, the adsorption energy of organic spacer onto perovskite is greatly reduced due to the strong intermolecular hydrogen bonding with ABS, contributing to a concentrated phase arrangement for smooth exciton energy transfer. Additionally, ABS modification further suppresses trap-mediated nonradiative recombination by coordinating with the undercoordinated lead (II) ions at grain boundaries. This work provides valuable guidelines for optimizing additive screening toward high-performance blue PeLEDs.
研究领域:
材料科学
·
光电子学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）