MATERIALS & DESIGN
·
2022
·
IF 9.417
Meng-Dan Gong,Xin-Jiang Pei,Guang-Xu Duan,Gao-Ying Zhi,Zhi-Qin Liu,Dong-Hao Zhang
To firmly immobilize enzyme on carrier surface without covalent attachment as well as improve enzyme stability, an effective method of enzyme immobilization was developed by encapsulating enzyme into nano-molecular cages on the surface of magnetic microspheres. Compared with physical adsorption, molecular cage encapsulation could fix lipase much more firmly and make lipase more active. Then, this study focused on investigating the effects of cage hydrophobicity and cage size on lipase encapsulation respectively by adjusting the log P value (from −0.85 to 1.28) and chain length (from 2.93 to 5.41 nm) of crosslinkers. It showed that lipase activity was dependent on the hydrophobicity of cage. Moreover, the size of cage had important effect on lipase loading amount, and a big cage was conducive to house lipase molecule. The structural analysis indicated that molecular cages could retain the active conformation of lipase and increase its structural rigidity. Molecular dynamics simulation revealed the interaction between the lid domain of lipase and molecular cage, leading to the higher activity of encapsulated lipase (63.6 U/mg) than that of free lipase (50.6 U/mg). Besides, the encapsulated lipase showed more than twice higher thermal stability and almost 8 times higher tolerance to denaturant than the adsorbed lipase.
研究领域:
生物化学
·
酶工程
✓ 已验证
产品关联
DOI
高影响因子
BIOCONJUGATE CHEMISTRY
·
2023
·
IF 4.7
Yun-Yan Xiao,Jiang-Tao Zhang,Yong-Hua Dong,Shu-Li Li,Gao-Ying Zhi,Ya-Fang Zhang,Dong-Hao Zhang
Here, lipase encapsulation is constructed by locking enzyme molecules in nanomolecular cages on the surface of SH-PEI@PVAC magnetic microspheres. To improve the encapsulation efficiency in enzyme loading, the thiol group is efficiently modified on the grafted polyethyleneimine (PEI) using 3-mercaptopropionic acid. N2 adsorption–desorption isotherms reveal the existence of mesoporous molecular cages on the microsphere surface. The robust immobilizing strength of carriers to lipase demonstrates the successful encapsulation of enzymes in nanomolecular cages. The encapsulated lipase shows high enzyme loading (52.9 mg/g) and high activity (51.4 U/mg). Different sizes of molecular cages are established, and the cage size showed important effects on lipase encapsulation. It shows that enzyme loading is low at a small size of molecular cages, which is attributed to that the nanomolecular cage is too small to house lipase. The investigation in lipase conformation suggests that the encapsulated lipase retains its active conformation. Compared with the adsorbed lipase, the encapsulated lipase shows higher thermal stability (4.9 times) and higher resistance to denaturants (5.0 times). Encouragingly, the encapsulated lipase shows high activity and reusability in lipase-catalyzed synthesis of propyl laurate, suggesting the potential application value of encapsulated lipase.
研究领域:
生物化学
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纳米技术
·
酶工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MATERIALS CHEMISTRY AND PHYSICS
·
2017
·
IF 2.084
Nan Jing,An-Nan Zhou,Ying-Qiao Xiang,Qing-Hong Xu
The chemical precipitation reaction of Ca 2+ and phosphate aqueous solution was used to prepare super-small hydroxyapatite (HA) nanoparticles in the presence of glucose. A hypothetical “molecular cage” was built in glucose alkaline solution to limit the chemical reaction in a tiny space to control the size and morphology of HA nanoparticles. A possible reaction mechanism of HA nanoparticles confined space synthesis was proposed in this work. Compared with the previous research of the preparation of HA nanoparticles, our work in the first time successfully obtained the surper-small HA nanoparticles which had a 7 nm average size with homogeneous globular morphology. The mild and green synthetic method achieve the low-cost and controllable preparation of super-small nanoscale HA particles. In addition, Zn(OH) 2 , Ni(OH) 2 , Ag and SiO 2 nanoparticles (<10 nm) were also successfully synthesized by this method, indicating “molecular cage” can be appropriate for various nanoparticle synthesis and could become a universally method for the synthesis of nanoparticles.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
·
2022
·
IF 6.76
Sisi Ma,Ligang Lin,Xinyang Li,Wenying Shi,Xiaofei Zhai,Jing Yang
Developing multifunctional, efficient and durable membrane for treating complex oily wastewater is highly desirable but still a challenge due to the severe membrane fouling . Herein, nanosponge membrane with 3D-macrocycle β-cyclodextrin (β-CDs) as molecular cage was manufactured by azide-alkyne click reaction for oil/water treatment and antifouling properties simultaneously. The macrocyclic ‘molecular cage’ geometry of β-CDs can induce various guest molecules into their cavities. When clickable β-CD-N 3 was fixed onto a clickable EVAL-≡ membrane surface, the hydrophilicity of the membrane was greatly improved. Furthermore, the molecular cage–grafted membrane (EVAL-g-CD) showed better antifouling performance than a pure EVAL membrane, with lower water flux decline (15%) and higher water flux recovery (91%). The flux and separation efficiency values of the EVAL-g-CD membrane were higher than 120 L·m −2 ·h −1 and 99%, respectively. The EVAL-g-CD membrane also exhibited good adsorption performance for organic pollutants owing to its cavity structure. Furthermore, the membrane showed desirable stability and its rejection remained at 99% after filtration. This proposed 3D membrane strategy based on molecular cages sheds light on the formation of hydrophilic membrane surfaces and shows great promise for potential applications such as the separation of oil-in-water emulsions.
研究领域:
材料科学
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环境工程
·
化学工程
✓ 已验证
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2020
·Vol. 45
·期 6
·3343-3348
·
IF 3.288
Ming Li,Yu Peng,Fei Yan,Chunguang Li,Yiqiang He,Yue Lou,Dingxuan Ma,Yi Li,Zhan Shi,Shouhua Feng
A novel cage-based crystalline covalent organic framework, i.e. Cage-COF-TT (TT = triammonia–terephthalaldehyde), was prepared from a prism-like triammonia-containing molecular cage and terephthalaldehyde. Its ordered structure and porosity are expected to ensure efficient drug delivery as a potent carrier. The excellent cell viability of Cage-COF-TT was testified through MTT assays with respect to both normal mouse cells and normal human hepatocyte cells. In terms of drug delivery performance, Cage-COF-TT exhibit a high loading capacity and efficient drug release profile in a simulated body fluid environment. This work offers new insights into the design and development of a COF-based drug carrier and inspires the development of versatile tools for biomedical applications.
研究领域:
材料科学
·
生物医学工程
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Litao Wang,Zhen Li,Ying Wang,Niannian Li,Dekuan Hu,Wei Wu,Jin xia Hu,Dong Pei,Mei Lv
The epoxy propanol molecular cage bonded silica stationary phase, RCC3-GLD@silica, synthesized through the ring-opening reaction of secondary amine with epoxy propanol using RCC3-R as the scaffold unit, was successfully prepared as confirmed by infrared spectroscopy, thermogravimetric analysis, and nitrogen adsorption-desorption characterization. This stationary phase demonstrated excellent separation performance in both reversed-phase and hydrophilic chromatography modes, effectively separating a wide variety of compounds including alkylbenzenes, polycyclic aromatic hydrocarbons, phenols, anilines, sulfonamides, nucleosides, amino acids, sugars, and acids. The development of RCC3-GLD@silica benefits from the synergistic effects of its hydrophobic and hydrophilic actions, as evidenced by the U-shaped characteristic of the retention factor for nucleoside compounds with changes in the aqueous content of the mobile phase, further confirming the simultaneous presence of reversed-phase and hydrophilic chromatography mechanisms. Not only did this stationary phase successfully separate 33 compounds in reversed-phase chromatography mode, but it also separated 54 compounds in hydrophilic interaction chromatography mode, showcasing its broad separation capability from weakly polar to strongly polar compounds on a single chromatographic column. This indicates a wide application prospect in the field of chromatographic analysis.
研究领域:
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Yi-Ping Cao,Gao-Ying Zhi,Li Han,Queting Chen,Dong-Hao Zhang
To improve the performance of lipase in biosynthesis of benzyl cinnamate, a new immobilized lipase by entrapping enzyme into nano-molecular cages was designed. Consequently, the entrapped lipase showed a robust immobilization, which diminished the leakage of lipase notably in use. Moreover, the entrapped lipase exhibited higher activity (57.1 U/mg) than free lipase (50.0 U/mg), demonstrating that the native conformation of lipase was not destroyed during immobilization. Compared with the adsorbed lipase (half-life 40.7 min) and free lipase (half-life 29.8 min), the entrapped lipase (half-life 85.3 min) increased the stability by about 2–3 times. Furthermore, the entrapped lipase was applied in biosynthesis of benzyl cinnamate, where it showed excellent activity and re-usability. After 7 cycles, the yield of benzyl cinnamate catalyzed by the entrapped lipase remained 70.2%, while the yield catalyzed by the adsorbed lipase was only about 10%. These results indicated that the nano-molecular cages could inhibit denaturation of lipase and maintain its activity well.
研究领域:
生物化学
·
酶工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2020
·Vol. 56
·期 1
·380-391
·
IF 3.553
Hou Ying,Nie Bei
Abstract With an in situ synthesized AgNO 3 complex molecular cage, icosahedral C 60 can be selectively extracted from fullerene mixture by a facial liquid–liquid interfacial precipitation, resulting in exclusive C 60 organometallic microcrystal (C 60 /C 70 = 6/1). By exploiting its intrinsic insolubility against nonpolar solvent, the C 60 can be separated from the homologues, C 70 , which contrarily is assembled through merely hydrophobic interaction and π-π stacking. The AgNO 3 complex encaged C 60 crystal was morphologically and structurally characterized by SEM, Raman and XRD spectra to revealing the underlying separation mechanism. The selective extraction efficiency (89.7%) was semiquantitatively characterized by combined optical spectroscopy and mass spectrometry, indicating a decent specificity of spontaneously formed molecular cage toward C 60 extraction. Thus, this discovery of a template-specific molecular cavity would pave the way to advance molecular imprinting technology using supramolecular array for fullerene separation, prevailing over the conventional “design-synthesis-performance” strategy by substantially reducing the laborious work and economic cost. Graphic abstract
研究领域:
化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2016
·
IF 2.567
Yu Liu,Weidong Chi,Donglin Zhao,Hui Liu,Yu Deng
The molecular-cage method is a convenient and universal way of synthesizing nanoparticles in aqueous phase. In this paper, hydroxyapatite (HA) and zirconium hydroxide nanoparticles are made by the molecular-cage method with a hydroxyl-rich solute. The structure, morphology, and particle size of the product were analyzed by differential scanning calorimetry and scanning electron microscopy. The chemical structures of the particles are not affected by the molecular-cage effect. The sizes of the nanoparticles and the concentration of the solute have a corresponding relationship in a limited range. The higher the concentration of the solute, the smaller the nanoparticles. Besides, HA nanoparticles are also made by the molecular-cage method in a water/ethanol mixture.
研究领域:
材料科学
·
纳米技术
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 43
·期 4
·1699-1709
·
IF 3.201
Chunfang Zhang,Xuejian Si,Shuo Zhang,Bei Pei,Jin Gu,Yunxiang Bai
Porous materials offer an attractive pathway to enhance the nanofiltration performance of thin film nanocomposite (TFN) membranes. Widely studied porous materials, such as zeolite, and metal–organic frameworks suffer from interfacial defects and poor dispersion in a polymer matrix, which leads to less than optimal performance of the TFN membranes. Porous metal–organic molecular cages are an exciting new family of porous materials that could ameliorate these issues by their better dispersing ability and intimate integration within the polymer matrix. By using the interfacial polymerization (IP) process, we synthesized a novel polyamide (PA) TFN membrane with highly improved nanofiltration performance using porous iron–organic molecular cage (Fe-cage) as the filler. Owing to the porous tetrahedral structure and polar –SO3Na group of the Fe-cage, the as-synthesized PA/Fe-cage TFN membranes at 1.0 wt% Fe-cage concentration in the aqueous solution showed water flux up to 26.33 L m−2 h−1, which was 3 times more than that of neat PA thin film composite (TFC) membranes, while simultaneously increasing the rejection ratio to 89.3% for Na2SO4 and 96% for Congo red. Further, the addition of Fe-cages induced a significant improvement in the antifouling property of the membranes, which could be attributed to both increased hydrophilicity and smooth surface microstructure of the membranes. These results provide a starting point for the high-performance TFN NF membranes by utilizing porous metal–organic molecular cages.
研究领域:
材料科学
·
环境工程
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Ruifang Ding,Sheng Liu,Yangyang Zhang,Zhen Li,Ying Zuo,Dong Pei,Tao Lan,Jinxia Hu,He Zhu,Mei Lv,Litao Wang
The separation and analysis of complex samples with wide polar range on a singular column is always a difficult problem in separation and analysis science. In this study, the RCC3-R molecular cage modified with the quaternary ammonium salt stationary phase (RCC3-GQ@silica) was successfully prepared and applicated in the separation of wide polar range compounds. In the Reverse Phase Liquid Chromatography (RPLC) mode, due to the hydrophobicity and π-π interactions provided by the cyclohexane and benzene rings in the molecular cage structure, this stationary phase demonstrated effective separation capabilities for alkylbenzenes, polycyclic aromatic hydrocarbons, phenols, and anilines. In the Hydrophilic Interaction Liquid Chromatography (HILIC) mode, the study explored the separation performance of this stationary phase for sugars and inorganic salts. Utilizing a mixed mode of HILIC/RPLC/Ion Exchange Chromatography (IEC), effective separation was achieved for sulfonamides, nucleosides, acids, and amino acids, indicating good separation effects for medium to strongly polar compounds as well as various hydrophilic compounds. Combining various separation modes, the RCC3-GQ@silica stationary phase successfully separated 91 compounds across 15 categories. These results not only demonstrate the potential of the stationary phase in expanding the range of polarities of analyzable compounds and achieving multipurpose use on a single column, but also confirm its effective separation of nucleosides in pure water systems, further emphasizing the significant application potential of RCC3-GQ@silica stationary phase in the field of green chemistry.
研究领域:
分析化学
·
分离科学
·
绿色化学
✓ 已验证
DOI
PubMed
高影响因子
Biomaterials Advances
·
2024
·
IF 5.5
Hu Hui,Yuheng Song,Hongmei Liu,Jiahui Fan,Zhou Sha,Hongchuang Li,Jian Lu,Qian Zhang,Xiang Fei,Meifang Zhu
Artificial periosteum is deemed a novel strategy for inducing endogenous bone regeneration, but ideal periosteum substitutes achieved by orchestrating a biomimetic microenvironment for bone regeneration remain a significant challenge. Here, we design and fabricate a hybridized nanofiber-based artificial periosteum with boosted osteoinduction properties. Via a “molecular cage” biomineralization strategy, nano-hydroxyapatite (nano-HAp) with a controllable size (∼22 nm) and excellent dispersion serves as unique nano-additives for water-soluble polyvinyl-alcohol (PVA)-based artificial periosteum. The PVA/HAp composite is electrospun into nanofibers to replicate the extracellular-matrix-inspired nanostructure for inducing cell adhesion, proliferation, and fate manipulation. A simple post-crosslinking treatment is subsequently applied to further booster its mechanical strength (6.6 MPa) and swelling stability. The optimized sample of C-PVA/HAp (10 wt% nano-HAp) artificial periosteum features excellent biocompatibility and remarkable in vitro mineralization. Cell experiments demonstrate that its effectively boasted cell modulation for enhanced osteogenesis without the aid of growth factors, showing a possible activation of the ERK/MAPK signaling pathway. This work provides an effective strategy for designing novel HAp nano-additives and expands the possibility of biomimetic fabrication for more advanced nanofiber-based artificial periosteum.
研究领域:
生物材料
·
组织工程
·
骨再生
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）