Juan Zhou,Huiyan Ying,Mingyu Wang,Dandan Su,Guozhong Lu,Jinghua Chen
A dual layer conduit, which consists of collagen I-hyaluronic acid (Col-HA, external layer) and collagen I-heparin (Col-HP, inner layer), has been developed. This conduit not only meets the structural requirement of nature blood vessel, but also presents favourable mechanical performance after crosslinking via EDC. In addition, its biological properties are evaluated through culturing with fibroblast cell (Cos-7) on Col-HA surface and vascular endothelial cell (HMEC) on Col-HP layer. Results demonstrate that the conduit can support Cos-7 and HMEC adhesion, proliferation and elongation on respective side. This dual layer conduit would be a new choice of artificial vascular scaffold and improve blood vessel regeneration for tissue engineering.
Surface morphological instability, induced by geometric incompatibility, is ubiquitous in nature and plays a pivotal role in maintaining specific biological functions in organs. In this study, we experimentally reveal that geometric incompatibility effectively regulates the morphological instability of bilayer tubes. Building upon these experimental findings, we develop a theoretical model to elucidate the underlying mechanical mechanism of the observed morphological instability. Theoretical analysis demonstrates that by manipulating geometric incompatibility, various patterns including circumferential, two-dimensional (2D), and axial patterns can be generated in bilayer tubes. Specifically, as the axial geometric incompatibility parameter increases, the instability pattern transitions from the circumferential pattern to the 2D pattern and eventually to the axial pattern. Furthermore, we conduct a series of quantitative experiments and finite element simulations to validate our theoretical model. Both the numerical simulations and experimental observations show excellent agreement with our theoretical predictions, demonstrating that linear stability analysis can be employed not only to predict wrinkles but also to determine the number of creases in tubular soft materials roughly. This study significantly advances our understanding of the morphological instability of geometrically incompatible bilayer tubular tissues and provides valuable insights for the fabrication of morphology-related multifunctional surfaces.
危险品化学品经营许可证（带存储）