JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Jiali Yang,Xia Jiang,Rong Zhang,Qinxiang Luo,Bin Wang,Xuewei Shi,Zhangying Wang
The quality of table-stock sweetpotatoes is significantly influenced by carbohydrates. The study investigated the changes of dry matter, starch, amylose, soluble sugar and 22 sugars in six purple-fleshed sweetpotatoes (PFSPs) with different anthocyanin contents harvested at 75, 105, 135, 165 days after transplanting, and revealed the accumulation pattern of carbohydrates in PFSPs. The dry matter accumulated constantly. The starch content decreased at 165d in GZ1, GZ9, GZ10 and G95–1, while GZ2 and G20 continued to accumulate with more starchiness taste. The accumulation of soluble sugars was primarily observed in 75–135 days, with sucrose, glucose, and D-fructose representing the predominant sugars. The proportion of sucrose varying from 41.75 % to 87.07 %, but decreased at 165d accompanying with the increasing of glucose and fructose. GZ1 exhibited the most favorable taste profile with higher sweetness and GZ10 accumulated sugar rapidly at 165d, making it the most suitable for harvesting later. While GZ2, GZ9, G20 and G95–1 accumulated carbohydrates rapidly in 75–135 days, which can be harvested appropriately early. This study can provide a theoretical basis for the selection and breeding for better quality of PFSPs.
研究领域:
植物学
·
食品科学
✓ 已验证
DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2024
·
IF 7.6
Qian Liu,Shuyuan Wang,Wei Wang,Jie Chen,Lizhong Zhu
Halogenated flame retardants in farmlands were observed to inhibit the growth of exposed crops. This study aimed to elucidate the mechanism of inhibition on rice by employing four representative polybrominated diphenyl ethers (PBDEs) and polychlorinated biphenyls (PCBs). The exposure to these contaminants at 200 nM led to a decrease of 0.63–0.95 fold in rice below-ground biomass and 0.49–0.66 fold in yield, and a corresponding 4%–10% increase in soluble sugars in leaves. PBDEs and PCBs were found to significantly disrupt the synthesis, decomposition, and transport of sugars in leaves, the three pivotal determinants of crop growth. Notably, these compounds promoted a 1.41- to 7.60-fold upregulation of the triose phosphate translocator, significantly enhancing soluble sugar synthesis. Conversely, a 0.45–0.97 fold downregulation was observed for sucrose transporters, thus impeding the leaf-to-shoot efflux of soluble sugars. Furthermore, PBDEs and PCBs were favorably bound to fructose-1,6-bisphosphate aldolase (FBA), inducing its substrate-specific dysfunction in fructose-1,6-diphosphate decomposition (3%–14%). Overall, PBDE and PCB exposure promoted a notable intracellular accumulation of soluble sugars in rice leaves, a typical symptom of plant diabetes, since the intensified synthesis of soluble sugars in leaves and the repressed decomposition and transportation of soluble sugars to other storage organs, thus impeding crop growth. This study provided an insightful understanding of the toxic effects and molecular mechanisms of halogenated flame retardants, highlighting their role in abnormal sugar accumulation and growth inhibition in crops and offering vital information for the risk assessment and administration of these compounds to guarantee the safety of agricultural products.
研究领域:
环境科学
·
植物生理学
·
毒理学
✓ 已验证
DOI
PubMed
高影响因子
CyTA-Journal of Food
·
2018
·
IF 1.371
Liang Li,Ying Ma
Methyl ketones can be formed by the metabolism of intermediate products of β-oxidation by enzymes. In this article, the effect of soluble saccharides on the activity of key enzymes involved in methyl ketone synthesis in L. lactis was studied by detecting the relationship between soluble saccharides and enzyme activity. The results showed that glucose, fructose, sucrose, lactose and galactose could promote the growth of L. lactis. Glucose induced the activity of enoyl-coenzyme A (CoA) hydratase but inhibited the activity of β-hydroxyacyl-CoA dehydrogenase. Fructose induced the activities of acyl-CoA dehydrogenase and enoyl-CoA hydratase while maintaining the activity of β-hydroxyacyl-CoA dehydrogenase. Sucrose induced the activities of enoyl-CoA hydratase and β-hydroxyacyl-CoA dehydrogenase and maintained the activity of thiolase. Lactose also induced enoyl-CoA hydratase activity. Galactose induced enoyl-CoA hydratase activity and maintained thiolase activity. Glucose, lactose, fructose and galactose induced thioesterase activity. The data showed fructose and lactose could inhibit thiolase activity and induce thioesterase activity, which would be beneficial to methyl ketone synthesis, so fructose and lactose were the best soluble saccharides, followed by galactose, glucose and sucrose. A positive and significant correlation was found between thioesterase activity and enoyl-CoA hydratase activity (p < 0.01). In summary, the β-oxidation system of L. lactis is related to sugar metabolism.
研究领域:
微生物学
·
生物化学
✓ 已验证
DOI
高影响因子
Plants-Basel
·
2025
·Vol. 14
·期 8
·1191
·
IF 4.1
Wenjing Qi,Jinping Bai,Han Yu,Guojun Han
Background: Heavy metal contamination significantly threatens crop growth and global food security. Understanding plant responses to such stress is crucial to developing stress-tolerant crops. This study explores the physiological and biochemical responses ofVigna radiata(L.) R. Wilczek to mercury, lead, and copper stress, focusing on the role of soluble sugar accumulation and biomass enhancement in conferring heavy metal tolerance. Methods: Commercially availableV. radiataseeds were exposed to varying concentrations (50, 150, and 300 mg/L) of mercurous nitrate, lead nitrate, and copper chloride under controlled conditions. The germination rates, seedling growth, and physiological parameters such as the soluble sugar and protein content were analyzed using spectrophotometry and statistical methods, including ANOVA. Results: The results demonstrated that lead ion stress significantly increased the seedling dry weight, while all the tested heavy metals promoted soluble sugar accumulation. Although the heavy metals inhibited germination and growth at higher concentrations,Vigna radiataexhibited strong tolerance at moderate stress levels. Conclusion: This study highlights the adaptive strategies ofV. radiata, including soluble-sugar-mediated osmotic adjustment and enhanced biomass allocation, which contribute to its resilience under heavy metal stress. These findings provide insights for breeding stress-resistant crops and managing heavy-metal-contaminated environments.
研究领域:
植物生理学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
·
2019
·Vol. 66
·期 5
·806-817
·
IF 0.94
An Y. Q.,Sun L.,Wang X. J.,Sun R.,Cheng Z. Y.,Zhu Z. K.,Yan G. G.,Li Y. X.,Bai J. G.
Abstract This study investigated whether vanillic acid (VA) mitigated dehydration stress responses in blueberry ( Vaccinium corymbosum L.), and analyzed potential mechanisms mediating this activity. We pretreated 2‑year-old blueberry plants with 40 μM VA for two days, and then induced dehydration stress by irrigating with nutrient solution containing 10% (w/v) polyethylene glycol 6000 for two days. VA pretreatment increased the transcript levels of genes encoding eight antioxidant enzymes in leaves, including iron superoxide dismutase, chloroplast copper/zinc superoxide dismutase, cytoplasmic copper/zinc superoxide dismutase, catalase, guaiacol peroxidase, glutathione peroxidase, glutathione reductase, and dehydroascorbate reductase. These increased transcript levels were consistent with enhanced activities of superoxide dismutase and glutathione peroxidase and elevated contents of reduced glutathione and ascorbate. Subjecting the V--A‑treated blueberry to dehydration stress further enhanced expression levels of these genes, compared with the control plants subjected only to dehydration stress, increased the contents of endogenous VA, proline, and soluble sugars, enhanced the relative water content and osmotic potential, and reduced the levels of superoxide anion, hydrogen peroxide, and malondialdehyde. So pretreatment of blueberry with VA reduces lipid peroxidation and mitigates dehydration stress by enhancing the endogenous VA content, activating the expression of antioxidant enzyme genes, and increasing the levels of proline and soluble sugars.
研究领域:
植物生理学
·
胁迫生物学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Li Wang,Timin Shan,Bing Xie,Chen Ling,Shuang Shao,Peng Jin,Yonghua Zheng
Glycine betaine (GB) treatment is useful to reduce chilling injury (CI) of several kinds of fruits including peach. However, the regulatory mechanism remains unknown. In this study, peach fruit was treated with 10 mmol L −1 GB solution for 10 min. The effects of GB treatment on CI, phenolic and soluble sugar metabolism were investigated in this study. Moreover, phenylpropanoid and soluble sugar content, and enzyme activities associated with phenolic and sugar metabolisms were also measured. The results showed that GB reduced CI and maintained high levels of total phenolic and flavonoid content. The activities of phenylpropanoid metabolism-related enzymes were significantly enhanced by GB. Higher content of sucrose and lower contents of fructose and glucose were observed in GB-treated fruits. Therefore, our results showed that GB could enhance chilling tolerance of peach through regulating phenolic and sugar metabolisms, and maintaining high levels of individual phenolic and sucrose content.
研究领域:
植物生理学
·
食品科学
·
农业化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Minglan Xu,Narcisse Tsona Tchinda,Siyang Li,Lin Du
The chemical composition of aerosols plays a significant role in aerosol-cloud interactions and, although saccharides make up their largest organic mass fraction, the current process model for understanding sea spray aerosol (SSA) composition struggles to replicate the enrichment of saccharides that has been observed. Here, we simulated the generation of SSA and quantified the enrichment of two soluble saccharides (glucose and trehalose) in SSA with a homemade sea spray aerosol generator. The results of the generation experiments demonstrated that both saccharides, especially trehalose, can promote the generation of SSA, whereas surface-active fatty acids primarily inhibit SSA production due to fewer bubble bursts caused by a large amount of foam accumulation. A significant decrease in surface tension of seawater with the addition of fatty acids was observed, while only a minor decrease was observed for seawater with the addition of only saccharide. Enrichment factors (EFs) of saccharides measured using high performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD) revealed no enrichment of glucose in submicron SSA, while trehalose showed a slight enrichment. In the presence of surface-active fatty acids on the seawater surface, a significant increase in the enrichment of saccharides in SSA was observed, with glucose and trehalose showing EF of approximately 27-fold and 58-fold, respectively. Besides, this enrichment was accompanied by the accumulation of calcium and magnesium ions. The results presented here suggest that the coupling interaction mechanism of soluble saccharides and surface-active fatty acids on the ocean surface contributes to the enrichment of soluble saccharides in SSA.
研究领域:
大气科学
·
海洋化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHYSIOLOGIA PLANTARUM
·
2018
·Vol. 165
·期 2
·155-168
·
IF 2.58
Wasim Ibrahim,Cheng-Wei Qiu,Can Zhang,Fangbin Cao,Zhu Shuijin,Feibo Wu
Soil salinity and drought are the two most common and frequently co-occurring abiotic stresses limiting cotton growth and productivity. However, physiological mechanisms of tolerance to such condition remain elusive. Greenhouse pot experiments were performed to study genotypic differences in response to single drought (4% soil moisture; D) and salinity (200 mM NaCl; S) stress and combined stresses (D + S) using two cotton genotypes Zhongmian 23 (salt-tolerant) and Zhongmian 41 (salt-sensitive). Our results showed that drought and salinity stresses, alone or in combination, caused significant reduction in plant growth, chlorophyll content and photosynthesis in the two cotton genotypes, with the largest impact visible under combined stress. Interestingly, Zhongmian 23 was more tolerant than Zhongmian 41 under the three stresses and displayed higher plant dry weight, photosynthesis and antioxidant enzymes activities such as superoxide dismutase (SOD), peroxidase (POD) catalase (CAT) and ascorbate peroxidase (APX) activities compared to control, while those parameters were significantly decreased in salt-stresses Zhongmian 41 compared to control. Moreover, Na + /K + -ATPase activity was more enhanced in Zhongmian 23 than in Zhongmian 41 under salinity stress. However, under single drought stress and D + S stress no significant differences were observed between the two genotypes. No significant differences were detected in Ca 2+ /Mg 2+ -ATPase activity in Zhongmian 41, while in Zhongmian 23 it was increased under salinity stress. Furthermore, Zhongmian 23 accumulated more soluble sugar, glycine-betaine and K + , but less Na + under the three stresses compared with Zhongmian 41. Obvious changes in leaf and root tips cell ultrastructure was observed in the two cotton genotypes. However, Zhongmian 23 was less affected than Zhongmian 41 especially under salinity stress. These results give a novel insight into the mechanisms of single and combined effects of drought and salinity stresses on cotton genotypes.
研究领域:
植物生理学
·
农学
·
胁迫生物学
✓ 已验证
DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 15
·期 1
·
IF 5.1
Huanling Lan,Xueyuan Lin,Huanhuan Ma,Liuying Lu,Wenxia Liao,Yuting Wang,Yi Chen,Chang Li
Camellia oleiferaoil (CO) is an important edible oil with excellent nutritional value. Recently, there has been an increasing market demand for oils with distinct flavor profiles. However, the formation mechanisms of characteristic aromas in CO remain unclear. Therefore, this study investigated the effects of roasting (170 °C, 0–30 min) on free amino acids, soluble sugars, and volatile components in camellia seeds and the corresponding oils. To further elucidate the generation mechanisms of flavor compounds in CO, reaction systems simulating the Maillard reaction and lipid oxidation were constructed. The results show strong correlations between volatile compounds and both soluble sugars and free amino acids during roasting. The key flavor precursors identified included arginine, glutamic acid, glycine, histidine, leucine, phenylalanine, and lysine, as well as sucrose and glucose. The simulated systems indicated that the flavor compounds in CO were mainly derived from the Maillard reaction and lipid oxidation, with significant interactions enhancing its unique flavor. This study potentially provides scientific guidance for the production and flavor control of fragrant CO.
研究领域:
食品科学
·
风味化学
·
脂质研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Xuchao Yin,Yuming Wei,Tiehan Li,Jixin Zhang,Li Zou,Qingqing Cui,Chengye Lu,Jingming Ning
Maillard reactions occurred during the roasting process of large-leaf yellow tea (LYT) were the main reason for the formation of their unique flavor. However, the contribution of different amino acids and soluble sugars to the formation of heterocyclic compounds in the Maillard reaction is still unclear. First, this study found that trimethylpyrazine, 2-ethyl-3,5-dimethylpyrazine, 3-ethyl-2,5-dimethylpyrazine and 2,5-diethylpyrazine, which had higher odor activity values (OAV), were significantly increased with the increase of roasting temperature. In contrast, only five amino acids ( l -theanine, glutamic acid, lysine, glutamine, histidine) and three monosaccharides ( d -glucose, d -fructose, and l -fucose) decreased significantly with increasing roasting temperature, which were significantly and positively correlated with each other. This indicates that these five amino acids and three monosaccharides were involved in the Maillard reaction during the roasting process. In addition, the model thermal reaction results showed that lysine contributed significantly to the formation of pyrazine especially with d -glucose to methylpyrazine, 2,5-dimethylpyrazine, trimethylpyrazine and 3-ethyl-2,5-dimethylpyrazine, while l -theanine contributed significantly to the formation of 1-ethylpyrrole-2-carbaldehyde. The d -glucose and d -fructose were the main contributors to the Maillard reaction among soluble sugars. This study lays the foundation for future studies on the relationship between volatile compounds and Maillard reactions.
研究领域:
食品科学
·
茶叶化学
·
风味化学
✓ 已验证
产品关联
DOI
高影响因子
PLANT PHYSIOLOGY
·
2024
·
IF 6.5
Chu Le-Le,Zheng Wei-Xuan,Liu Hai-Qiang,Sheng Xing-Xing,Wang Qing-Ye,Wang Yue,Hu Chun-Gen,Zhang Jin-Zhi
Flowering is an essential process in fruit trees. Flower number and timing have a substantial impact on the yield and maturity of fruit. Ethylene and gibberellin (GA) play vital roles in flowering, but the mechanism of coordinated regulation of flowering in woody plants by GA and ethylene is still unclear. In this study, a lemon (Citrus limon L. Burm) 1-aminocyclopropane-1-carboxylic acid synthase gene (CiACS4) was overexpressed in Nicotiana tabacum and resulted in late flowering and increased flower number. Further transformation of citrus revealed that ethylene and starch content increased, and soluble sugar content decreased in 35S:CiACS4 lemon. Inhibition of CiACS4 in lemon resulted in effects opposite to that of 35S:CiACS4 in transgenic plants. Overexpression of the CiACS4-interacting protein ETHYLENE RESPONSE FACTOR3 (CiERF3) in N. tabacum resulted in delayed flowering and more flowers. Further experiments revealed that the CiACS4–CiERF3 complex can bind the promoters of FLOWERING LOCUS T (CiFT) and GOLDEN2-LIKE (CiFE) and suppress their expression. Moreover, overexpression of CiFE in N. tabacum led to early flowering and decreased flowers, and ethylene, starch, and soluble sugar contents were opposite to those in 35S:CiACS4 transgenic plants. Interestingly, CiFE also bound the promoter of CiFT. Additionally, GA3 and 1-aminocyclopropanecarboxylic acid (ACC) treatments delayed flowering in adult citrus, and treatment with GA and ethylene inhibitors increased flower number. ACC treatment also inhibited the expression of CiFT and CiFE. This study provides a theoretical basis for the application of ethylene to regulate flower number and mitigate the impacts of extreme weather on citrus yield due to delayed flowering.
研究领域:
植物生物学
·
分子遗传学
·
园艺学
✓ 已验证
DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 10
·3068
·
IF 4.927
Wenyun Wang,Song Liu,Mingyan Yan
Salinity is one of the major abiotic stresses limiting crop growth and productivity worldwide. Salt stress during germination degenerates crop establishment and declines yield in wheat, therefore alleviating the damage of salt stress to wheat seedlings is crucial. Chitooligosaccharide (COS) was grafted with γ-aminobutyric acid based on the idea of bioactive molecular splicing, and the differences in salt resistance before and after grafting were compared. The expected derivative was successfully synthesized and exhibited better salt resistance-inducing activity than the raw materials. By activating antioxidant enzymes such as superoxide dismutases (SOD), catalase (CAT) and phenylalanine ammonia-lyase (PAL) and subsequently eliminating reactive oxygen species (ROS) in a timely manner, the rate of O−2production and H2O2content of wheat seedlings were reduced, and the dynamic balance of free radical metabolism in the plant body was maintained. A significantly reduced MDA content, reduced relative permeability of the cell membrane, and decreased degree of damage to the cell membrane were observed. A significant increase in the content of soluble sugar, maintenance of osmotic regulation and the stability of the cell membrane structure, effective reduction in the salt stress-induced damage to wheat, and the induction of wheat seedling growth were also observed, thereby improving the salt tolerance of wheat seedlings.Keywords:chitooligosaccharide;γ-aminobutyric;salt stress
研究领域:
植物科学
·
农业生物技术
·
逆境生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）