Frontiers in Bioengineering and Biotechnology
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2025
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IF 4.8
Yaping Ma,Minchang Liu,Ruifang Gu,Rongya Zhang,Xiaomei Ji,Juan Zhang,Wu Wen,Zheng Peng
Introduction: Laccase exhibits significant applications in food additives, wastewater treatment, and biomass processing. Fungal laccase exhibits high activity, good stability, and excellent performance. However, scale-up production of high-yield laccase from fungi is challenging. This study aimed to identify crucial factors affecting enzyme production and analyze the enzymatic properties of laccase during fermentation.Methods: A laccase-producing white-rot fungus was used for fermentation process optimization in 200 L and 1200 L fermenters. The Plackett–Burman design revealed three significant influencing factors: temperature, aeration ratio, and agitation speed. The steepest ascent experiment was used to approximate the maximum response region, followed by the establishment of a regression model between experimental factors and laccase activity using the Box–Behnken response surface methodology and optimal fermentation condition selection.Results: The optimal conditions for laccase production by Ganoderma lucidum fermentation were 30°C temperature, 0.66 aeration ratio, and 100 rpm agitation speed, achieving a maximum laccase activity of 214,185.2 U/L. Dissolved oxygen (DO) was a crucial factor for high laccase yield, and its maintenance at a high level in the fermentation system significantly enhanced the enzyme activity. Fermentation batches with varying laccase production levels exhibited a trend of decreasing pH and a subsequent increase in the mid-to-late fermentation stages. With decreased pH, the DO level started declining; when DO stabilized, the pH started to rebound, coinciding with the peak laccase activity, indicating a signal of fermentation endpoint in industrial production.Discussion: This study provides valuable theoretical and data support for the industrial production of laccase by fungi through optimized fermentation processes.
研究领域:
生物技术
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微生物学
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生化工程
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工业生物技术
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DOI
PubMed
高影响因子
JOURNAL OF AOAC INTERNATIONAL
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2025
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IF 1.7
Tang Dianchao,Jin Chen,Gan Liqin,Zhong Lanlan,Wang Zexi,Liu Qiqiong,Yang Yiling,Liu Ling,Huang Huilian
BackgroundTinosporae Radix is a valuable plant with applications in medicinal and edible fields. Alkaloids are one of the main active components of Tinosporae Radix, showing prominent antioxidant activity and great potential in healthcare fields.ObjectiveResponse surface method was applied to determine the optimal enrichment process of total alkaloids of Tinosporae Radix (TRTA). Antioxidant alkaloid components were screened by characteristic chromatogram, antioxidant analysis and molecular docking, so as to provide the basis for Tinosporae Radix to develop as a medicinal and edible homologous plant and antioxidant products.MethodsIn this study, a single-factor investigation experiment and a response surface optimization experiment were used to determine the optimal enrichment process of D101 macroporous resin. High-performance liquid chromatography (HPLC) was employed to establish the characteristic chromatogram of TRTA, after which chemometrics was applied to evaluate the data. The antioxidant activity of TRTA was assessed using DPPH and ABTS assays. Grey relational analysis was applied to explore the spectrum-effect relationship, and molecular docking techniques were combined to predict targets of antioxidant activity.ResultsThe best enrichment process of total alkaloids is presented as follows: water removal 3 BV, 10% ethanol removal 3 BV, and 30% ethanol elution 3 BV. And all four identified alkaloid components contributed to the antioxidant activity of TRTA, potentially processing binding interactions with multiple antioxidant protein targets, including TNF, NO-1 and XOD.ConclusionsAlkaloids significantly contributed to the antioxidant activity of TRTA, with columbamine and palmatine playing particularly prominent roles. And the antioxidant activity of TRTA is likely exerted through a complex multi-component, multi-target mechanism, involving the coordinated action of various alkaloid components and multiple biological targets.HighlightsThe optimized enrichment process demonstrated remarkable efficiency in concentrating total alkaloids from Tinosporae Radix. This study contributes significantly to the formulation of its quality control methods.
研究领域:
植物化学
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分析化学
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药理学
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计算生物学
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DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
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2022
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IF 11.889
Rongxin Ling,Weiqi Wei,Yongcan Jin
In this work, HCl catalyzed ethylene glycol–water pretreatment (HCl/EG-H 2 O) of sugarcane bagasse (SCB) was explored with response surface methodology (RSM) and single factor analysis, which aim to investigate the influence of pretreatment variable on pretreated solid cellulose enzymatic conversion. The result showed that HCl/EG-H 2 O pretreatment could selectively extract ∼89.9 % xylan and ∼61.2 % lignin in SCB, meanwhile maintain a relatively high cellulose retention (∼86.8 %). Pretreatment of SCB at 120 °C for 60 min with 1.00 % HCl and 90 % EG obtained the pretreated solid having maximum cellulose enzymatic conversion of 88.7 % under 10 FPU/g enzyme dosage, this enhancement of cellulose enzymatic conversion mainly attributed to structure change of SCB in pretreatment. The adding of enzymatic additives into the hydrolysis process could not only improve hydrolysis efficiency but also lower the enzyme dosage. Besides, the linear relationship between substrate characteristic parameters (such cellulose content, lignin removal rate etc.) and cellulose conversion were observed.
研究领域:
生物技术
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可再生能源
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
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2025
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IF 6.3
Hao Peng,Jing Guo,Guixuan Jin,Jingjing Wang,Qiuyan Wu,Hongwei Liu,Aiyang Li
This study investigated the effectiveness of tartaric acid (TA) for the direct reduction of pentavalent vanadium (V(V)), a critical process in vanadium-related industries. Leveraging TA's carboxyl groups, which showed promise as reducing agents for V(V), the research systematically evaluated the effects of key parameters including reaction temperature, reaction time, H 2 SO 4 concentration, and TA dosage on reduction efficiency. Response surface methodology (RSM) analyzed parameter significance and interactions, while density functional theory (DFT) elucidated the molecular reduction mechanism. The process was further validated using an industrially sourced vanadium-rich solution. Results demonstrated that TA effectively reduces V(V) under elevated temperatures and TA dosage, achieving 100% within a short time under optimized conditions. RSM revealed all parameters positively influenced the reduction process, ranked by significance as: reaction temperature > reaction time > H 2 SO 4 concentration > TA dosage, underscoring the dominance of thermal activation. DFT analysis identified the molecular mechanism: the carbon atom within TA's carboxyl group (-COOH) donated lone pair electrons to V(V), facilitating reduction to V(IV). Successful application to the industrial vanadium solution confirmed TA's effectiveness. This study established TA as an efficient V(V) reducing agent, clarified its electron-transfer mechanism, and confirmed its dual role as both reductant and complexing agent.
研究领域:
无机化学
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环境科学
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工业化学
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冶金学
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绿色化学
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产品关联
DOI
高影响因子
APPLIED CLAY SCIENCE
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2025
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IF 5.8
Xiaolong Yan,Yuxuan Deng,Wanming Huang,Qijun Zhang,Li Zheng,Junfeng Zhou,Dongsheng He,Jiayu Ma
This study investigated LDHs-MgAl preparation factors on Gly adsorption performance. The single-factor experiments shown that CO 3 2− -type LDHs-400-MgAl prepared under the condition of calcination temperature of 400 °C, calcination time of 3 h, Mg/Al molar ratio of 2:1, synthesis pH of 10, preparation temperature of 30 °C and aging temperature of 25 °C, had better Gly adsorption performance due to its enhanced crystallinity and hierarchical porosity. Moreover, the Gly adsorption process optimization with LDHs-400-MgAl via the response surface methodology (RSM) was studied. The relative error between the actual value and the predicted value obtained from the RSM model is 0.39 % and the determination coefficient (R 2 ) is 0.9987, which means the Gly adsorption process with LDHs-400-MgAl can be predicted by the proposed RSM model. This work integrates material synthesis optimization with process engineering, offering a scalable strategy for Gly removal in complex wastewater matrices.
研究领域:
环境科学
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材料科学
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化学工程
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产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2022
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IF 4.598
Mingran Li,Xia Qin,Mingxiao Gao,Tinghui Li,Yue Lv
Electrosynthesis of hydrogen peroxide (H 2 O 2 ) by two-electron oxygen reduction on the cathode is an attractive green approach for environmental remediation and chemical industries. In this study, an active carbon fiber (ACF) electrode modified with graphite carbon nitride (g-C 3 N 4 ) and carbon nanotubes (CNTs) is fabricated by a wet ultrasonic impregnation-calcination method. The doping content of g-C 3 N 4 , CNTs and polytetrafluoroethylene (PTFE) is optimized by response surface methodology (RSM). With optimum doping content, the H 2 O 2 generation by modified ACF increased several times, indicating the physicochemical and electronic transformation of ACF. The introduction of g-C 3 N 4 modulates the electronic structure of ACF, alleviating the adsorption energy of oxygen and coordinating the ∗ OOH binding capability. Besides reducing the oxygen adsorption energy, CNTs simultaneously improve the conductivity of electrodes. In addition, the addition of an appropriate amount of PTFE constructs a gas–liquid-solid three-phase equilibrium, facilitating oxygen diffusion. The effects of operating parameters on H 2 O 2 generation are also investigated and the optimal performance is achieved at current = 0.2 A, pH = 3, and aeration rate = 0.32 L/min. The modified ACF shows great potential in the practical applications for in-situ H 2 O 2 electro-generation.
研究领域:
环境科学
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材料科学
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化学工程
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产品关联
DOI
高影响因子
TRIBOLOGY INTERNATIONAL
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2019
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IF 3.517
Bo Wu,Hui Song,Chuan Li,Ruhong Song,Tianming Zhang,Xianguo Hu
Nano-lanthanum hydroxide/reduced graphene oxide (nano-La(OH) 3 /RGO) composites were successfully prepared as anti-wear additives for diesel engine oil. Response surface methodology was adopted to explore the influences of temperature, contact pressure, and composite concentration on the tribological properties of diesel engine oil. The anti-wear mechanism of nano-La(OH) 3 /RGO composites was simultaneously studied by comparing with the tribological behavior of graphene oxide and nano-La(OH) 3 pure particles. Results indicate that nano-La(OH) 3 /RGO composites can remarkably improve the anti-wear performance of diesel engine oil under boundary lubrication conditions. In particular, the anti-wear performance of diesel engine oil increased by 44% after adding 0.1 wt% composites at a temperature of 80 °C and a contact pressure of 1.62 GPa. The synergistic anti-wear mechanism of graphene and lanthanum oxide was proposed on the basis of worn surface characterization .
研究领域:
材料科学
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机械工程
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产品关联
DOI
高影响因子
Bioresources and Bioprocessing
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2025
·Vol. 12
·期 1
·1-18
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IF 5.1
Zhang Rui,Wang Han,Wang Li,Zheng Zhiming
Menaquinone-7, a form of vitamin K 2 , plays a critical role in the treatment of hemorrhagic diseases caused by vitamin K deficiency and in the prevention of bone fractures. Microbial fermentation has emerged as a promising method for MK-7 production due to its high product optical purity and significant physiological activity. However, the current production efficiency of MK-7 remains insufficient to meet industrial demands. In this study, we employed a combination of single-factor experiments, Plackett-Burman design, steepest ascent experiments, and Box-Behnken design to optimize the fermentation medium for MK-7 production by Bacillus subtilis BS - Δ ackA . Through regression analysis and consideration of practical production constraints, the optimal fermentation medium parameters were determined as follows: 20 g/L sucrose, 20.7 g/L glycerol, 47.3 g/L soy peptone, 4 g/L yeast extract, and 1.9 g/L KH 2 PO 4 , 0.1 g/L MgSO 4 ·7 H 2 O. Under these optimized conditions, the MK-7 yield reached 154.6 ± 1.32 mg/L. The experimental results demonstrated excellent stability and reliability, aligning closely with the model predictions. This study significantly enhances MK-7 production at the shake-flask fermentation level, providing valuable insights for large-scale industrial applications.
研究领域:
微生物发酵
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生物技术
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生物工程
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应用微生物学
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营养科学
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产品关联
DOI
PubMed
高影响因子
BIOMASS & BIOENERGY
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2025
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IF 5.8
Xinyu Zhao,Yanjie Lv,Lijie Chen,Le Li,Xin Zhang,Zhongran Dai
Biochar materials are emerging as promising candidates for treating uranium-containing wastewater. In this study, the response surface methodology (RSM) and backpropagation (BP) neural network model were employed to optimize the processing parameters during the preparation of biochar via sludge aerobic fermentation products (SAPBC), thereby maximizing its potential in addressing the challenges associated with uranium-contaminated wastewater. Through systematic parameter optimization, the optimal pyrolysis conditions were determined as: pyrolysis temperature 382 °C, heating rate 5 °C/min, and holding time 2 h. Results obtained from batch adsorption experiments demonstrated that the maximum adsorption capacity of SAPBC-382 for U(VI) was 159.27 mg/g under a pH condition of 4.0. Kinetic analysis revealed the adsorption process followed pseudo-second-order kinetics ( R 2 = 0.992), while isotherm data best fitted the Langmuir model ( R 2 = 0.997), indicating monolayer chemisorption mechanism. Thermodynamic parameters confirmed the spontaneous and endothermic nature of the adsorption process. Notably, the SAPBC maintained >90 % removal efficiency through five consecutive adsorption-desorption cycles, demonstrating exceptional recyclability. Mechanism study reveals that the effective adsorption of U(VI) by SAPBC primarily stems from electrostatic attraction as well as the coordination of hydroxyl and carboxyl functional groups. Overall, this research adheres to the principle of waste control by waste not only provides an efficient solution for uranium-containing wastewater treatment but also establishes a sustainable approach for sludge valorization in environmental remediation applications.
研究领域:
环境工程
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化学工程
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核工程
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水处理技术
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废弃物管理
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产品关联
DOI
高影响因子
Geoenergy Science and Engineering
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2025
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IF 4.6
Muhammad Arqam Khan,Shaine Mohammadali Lalji,Moiz Ali Khan Sial,Areeba Batool,Mohsin Ayubi,Syed Imran Ali,Muhammad Mustafa,Mei-Chun Li
The increasing demand for sustainable carbon capture technologies necessitates the exploration of novel solvents for CO 2 absorption. Carbon Capture and Storage (CCS) is a critical technology for controlling greenhouse gas emissions and tackling climate change. This study investigated the efficiency of sodium alginate (Na-Alg) as a bio-based solvent in carbon capture technology using the simulation software, Aspen Plus. The model was developed to simulate the absorption of CO 2 in a tray column, and the performance of sodium alginate solution was analyzed using various key parameters, including temperature, pressure, and solvent flow rate, to maximize the carbon capture efficiency. The simulation was then validated with the help of existing literature and experimental data and was found to be in good correlation. The carbon capture efficiency of CO 2 was observed at 97 % using sodium alginate solution. The relations between process parameters and their influence on CO 2 capture were evaluated by applying response surface methodology, identifying optimal conditions at 10–20 °C, 10 bar pressure, and a solvent flow rate of 20–25 kmol/h. These findings highlight the potential of sodium alginate as an effective, eco-friendly solvent for CCS, offering both environmental and operational advantages. The study also demonstrates the utility of simulation and optimization techniques in enhancing CCS performance.
研究领域:
化学工程
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环境工程
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能源技术
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过程模拟
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产品关联
DOI
高影响因子
Desalination and Water Treatment
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2024
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IF 1
Zhiguo Xiao,Yue Cheng,Hongwei Yu,Jianyu Li
It is necessary to better separate and recover rare earth elements from acid solution. The adsorbent that magnetic tripod ligand was synthesized was used to selectively adsorb lanthanum (La (III)). The adsorbent was characterized by transmission electron microscope, X-ray diffraction and Fourier-transform infrared spectroscopy. The adsorption effect of the adsorbent was studied by single-factor experiments, wherein the single factors included dosage, time, interfering ions and pH. For better predicting the test results, we used response surface methodology (RSM) to analyze the interaction between every single factor. Also, experiments on adsorbent recovery were carried out, and the adsorption mechanism was briefly discussed. The results of the study indicated that the magnetic tripod ligand was successfully synthesized. The predicted removal rate of La(III) can reach 99.42% by RSM with the dosage of 0.8 g/L, time of 100 min, and pH of 5. Moreover, the experimental results showed that the removal rate of lanthanum ion could reach 97.9%. After five cycles of reuse of the adsorbent, the adsorption effect was reduced to 78%. The reason for the high removal of La(III) by the adsorbent may be the important role of coordination chelation.
研究领域:
环境科学
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材料科学
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化学工程
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分析化学
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DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 31
·76992-77005
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IF 5.8
Jia Qunpo,Li Bowen,Li Bo,Cai Yanpeng,Yuan Xiao
Sediment adsorption is one of the main environmental fates of neonicotinoids (NEOs) in aquatic environments. Little information is available on for the adsorption characteristics of NEOs on urban stream sediments. In this study, urban tidal stream sediments were collected to determine the adsorption properties of four selected NEOs. The influence of environmental factors on NEO adsorption was determined by the RSM-CCD method. The NEO adsorption rates on sediments were established by multiple regression equations. As a result, the adsorption of four NEOs onto sediments fitted a linear isotherm model. The adsorption amounts of thiacloprid (THA), clothianidin (CLO), acetamiprid (ACE), and imidacloprid (IMI) were 1.68 to 2.24, 1.71 to 2.89, 1.88 to 3.07, and 2.23 to 3.16 mg/kg, respectively. The adsorption processes of four NEOs on urban sediments were favorable. Moreover, adsorption behaviors of NEOs were typical physical adsorption and readily adsorbed onto urban sediments. The adsorption processes of NEOs were exothermic reactions, and their adsorption rates decreased with increasing pH. Flow rates and organic matter contents could promote the adsorption ratios of typical NEOs. Multiple linear regression was used to assess the relationships between the adsorption rates of NEOs and environmental factors. The p -values of the fitting equations of adsorption rates were all less than 0.05. Within the ranges of concentration of the investigated factors, the multiple regression equations were able to reasonably model and predict the sorption of typical NEOs onto urban stream sediments. Therefore, the adsorption rate equations effectively predicted the NEO adsorption performance of urban streams and were helpful for controlling risk assessment of NEOs.
研究领域:
环境科学
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生态毒理学
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水污染
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吸附过程
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城市水文学
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DOI
高影响因子
危险品化学品经营许可证（带存储）