COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
·
IF 5.4
Ruixue Yan,Hao Guo,Zeyun Yang,Wenting Niu,Jianpeng Ma,Liping Peng,Mingyue Wang,Wu Yang
Alterations in guanine (GA) and adenine (AE) levels within the human body are intricately linked to overall health and daily physiological functions. Therefore, it is crucial to develop an accurate and sensitive sensor for quantifying the levels of GA and AE. In this present paper, a novel electrochemical sensor NH 2 -MXene@COF TA-DH /GCE was constructed by in-situ growing COF TA-DH with high crystallinity, good thermal stability and N-rich active centers based on Schiff base condensation reaction of 1, 3, 5-tris-(4-aminophenyl) triazine (TAPT) and 2,5-dihydroxyl-terephthalaldehyde (DHTA) on highly conductive NH 2 -MXene and then modifying it on the surface of the glass carbon electrode (GCE). The highly dispersed sandwich structure of NH 2 -MXene@COF TA-DH significantly increases the specific surface area and exposes a greater number of active sites. Benefitted from remarkable synergistic effect, the sensor exhibited excellent electrochemical catalytic activity and high sensitivity for GA and AE detection. The sensing platform was successfully used for simultaneous detection of GA and AE with wide linear ranges of 0.6–230 μM and 1–450 μM and low detection limits of 0.07 μM and 0.44 μM, respectively. This study expands applications of COFs and MXene in electrochemical biosensing platform and provides a general and valuable strategy for COFs-based electrochemical sensor construction for biomedical investigations
研究领域:
生物医学
·
分析化学
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产品关联
DOI
高影响因子
BMC MICROBIOLOGY
·
2025
·Vol. 25
·期 1
·1-13
·
IF 4.2
Cao Xiaoyu,Zhang Yu,Xu Qianqian,Yan Hai
The overproduction of uric acid, driven by its key precursors (inosine and guanosine), leads to hyperuricemia, a metabolic disorder associated with severe complications such as gout and renal dysfunction. Here, a promising bacterial strain YC06 with excellent biodegradation capability for inosine and guanosine was successfully isolated from healthy individual feces and identified as Bacillus paranthracis through average nucleotide identity (ANI) analysis. B. paranthracis YC06 resting cells (live but suspended in PBS buffer) and its cell-free extracts could effectively biodegrade inosine and guanosine in vitro. Whole-genome sequencing revealed a 5,535,183 bp draft genome (52 contigs, 35.22% GC content) containing 5,672 protein-coding genes. B. paranthracis YC06 demonstrated high survival rates in simulated gastrointestinal fluids, supported by the presence of stress-response genes and bile salt hydrolase genes associated with gastrointestinal tolerance. However, this strain exhibited hemolytic activity and no amino acid decarboxylase activity, while hemolysin genes, antibiotic genes and toxin-producing genes were identified, raising potential biosafety concerns for its further application. The gene functional annotation and polymerase chain reaction (PCR) amplification electrophoresis identified key genes ( rihA , rihB , deoD and pnp ) encoding purine nucleosidase and purine-nucleoside phosphorylase, and combined with biodegradation product analysis, elucidated the pathways of inosine and guanosine biodegradation into hypoxanthine and guanine. Comprehensive safety evaluations, including cytotoxicity assay and in vivo pathogenicity studies, must be conducted to rigorously assess its risks prior to practical utilization.
研究领域:
微生物学
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医学
·
基因组学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2021
·
IF 4.539
Hao Guo,Tingting Zhang,Mingyue Wang,Lei Sun,Junye Zhang,Meng Yang,Fan Yang,Ning Wu,Wu Yang
In this work, a novel sensor based on gold nanoparticle-modified zirconium-metal-organic frameworks (MOF-801) and carboxyl-functionalized carbon nanotube (MWCNT-COOH) composite material was built successfully for electrochemical detection of guanine and adenine in DNA sample, which exhibits diverse advantages such as fast, convenience and high efficiency. Guanine and adenine were detected by the cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Under the optimal conditions, MOF-801/MWCNT-COOH/ AuNPs/GCE delivered linear DPV responses to guanine in the range of 0.8 ~ 60, 60 ~ 180 μM and the adenine in the range of 0.8 ~ 60, 60 ~ 180 μM with the low detection limit of 0.09 μM for guanine and 0.08 μM for adenine, respectively. The advantages of this method include simplicity, rapidity, high sensitivity, good selectivity as well as low cost. The modified GCE was successfully applied to the analysis of guanine and adenine in DNA samples.
研究领域:
生物化学
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分析化学
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产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2012
·
IF 2.905
Xiao Liu,Liqiang Luo,Yaping Ding,Qingsheng Wu,Youli Wei,Daixin Ye
In this study, a polymerized film of melamine (PMel) was first prepared on the surface of glassy carbon electrode (GCE) in acidic solution by cyclic voltammetry. Scanning electron microscopy, Fourier transforms infrared spectroscopy and electrochemical techniques were used for characterization of the modified electrode surface. The electrochemical activity of the poly-melamine film modified glassy carbon electrode (PMel/GCE) was primarily tested for electrochemical catalyzes of guanine (G), adenine (A) and epinephrine (EP) in 0.1 M phosphate buffer solution (pH 4.5). The oxidation currents of G, A, and EP at the PMel/GCE increased 9, 7 and 13 times respectively compared with that of bare GCE. In the detection of the three aforementioned analytes using square wave voltammetry, the linear ranges of the peak currents at the PMel/GCE were 0.1–50 μM G, 0.1–60 μM A and 0.1–100 μM EP with detection limits of 0.08 μM G, 0.07 μM A and 0.05 μM EP, respectively. Furthermore, the prepared electrode displayed voltammetric responses with high sensitivity, good selectivity and reproducibility for A, G, and EP in optimal conditions, making it very suitable for determination of the three components.
研究领域:
分析化学
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生物医学工程
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Yicheng Zhou,Rongrong Qiao,Li Dong,Jiazheng Zhang,Lin Liu,Chao Liu,Xiaobo Zhang,Zhiwei Tong
A nanocomposite of 5,10,15,20-tetrakis (N-methylpyridinium-4-yl) porphyrin cobalt (III) (CoTMPyP) and titanium oxide (Ti 4 O 9 2− ) nanosheets was constructed using a stripping/self-assembly method for the first time. The morphology, structure, and composition of CoTMPyP/Ti 4 O 9 2− were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and X-ray photoelectron spectroscopy (XPS). Electrochemical impedance spectroscopy (EIS), Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques were utilized to analyze the electrochemical properties and detect uric acid (UA), guanine (G), and adenine (A). Under optimized conditions (0.1 M phosphate buffer, pH 4.0), the CoTMPyP/Ti 4 O 9 2− modified glassy carbon electrode (GCE) enabled simultaneous detection of UA, G, and A with detection limits of 396, 59, and 47 nM, respectively (S/N = 3). The linear detection ranges were 0.31–16.99 μM for UA, and 0.12–4.02 μM for both G and A. The stability and anti-interference ability of the CoTMPyP/Ti 4 O 9 2− /GCE sensor were also examined. Further tests in serum samples demonstrated recoveries of 98.1–109.7 % for the biomolecules, with relative standard deviations of 0.8–6.2 %. These results indicate excellent performance of the nanocomposite sensor for electrochemical detection.
研究领域:
分析化学
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生物医学工程
·
材料科学
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产品关联
DOI
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2016
·Vol. 20
·期 7
·2055-2062
·
IF 2.327
Chen Yalin,Mei Tao,Chen Yi,Wang Jianying,Li Jinhua,Fu Yang,Dai Guangchao,Wang Sheng,Xiong Weilai,Wang Xianbao
In this study, we prepared the porphyrin/reduced graphene oxide (porphyrin/RGO) nanocomposite which combined the excellent electrical conductivity and high specific surface area of RGO and the catalytic effect on the oxidation of guanine and adenine of porphyrin for simultaneous detection of guanine and adenine. Since the nanocomposite had remarkable synergistic effect between porphyrin and RGO, the modified glassy carbon electrode (GCE) exhibited excellent electrochemical catalytic activities and high sensitivity toward the detection of guanine and adenine. The nanocomposite was successfully applied to simultaneous quantitative detection of guanine and adenine with a linear range covering 0.05–150 and 0.2–100 μM, respectively, and the detection limits (LOD) ( S / N = 3) were estimated to be 0.016 μM for guanine and 0.06 μM for adenine. The porphyrin/RGO/GCE was applied for simultaneous detection of trace amounts of guanine and adenine in hydrochloric calf thymus DNA. Moreover, the porphyrin/RGO/GCE was simply fabricated and displayed good reproducibility.
研究领域:
分析化学
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生物医学工程
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材料科学
✓ 已验证
DOI
高影响因子
ELECTROCHEMISTRY COMMUNICATIONS
·
2019
·
IF 4.197
Jian Zhang,Dong Han,Shu Wang,Xiaodi Zhang,Ruiqi Yang,Yanchen Ji,Xin Yu
In this work, a WS 2 nanosheet/graphite microfiber hybrid electrode has been fabricated by in situ synthesis of WS 2 nanosheets on the surface of graphite microfibers. The WS 2 nanosheets possess an excellent electrocatalytic oxidation response towards adenine and guanine that encourages the adsorption of nucleobases and charge transfer at the surface of the microfibers. The WS 2 nanosheet/graphite microfiber hybrid electrode shows high sensitivity and selectivity for the detection of adenine and guanine. This hybrid microfiber electrode represents an excellent candidate platform for development of an electrochemical biosensor due to its low cost, flexibility and 3D morphology.
研究领域:
生物化学
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材料科学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
Soft Matter
·
2024
·Vol. 20
·期 22
·4422-4433
·
IF 2.9
Xiubin Hou,Yingxia Wang,Xinbing Song,Juan Gao,Yurong Ma
Biogenic purine crystals can function in vision as light scatters, mirrors, and multilayer reflectors and produce structural colors or depolarization for camouflage. Xanthine crystals form irregular multifocal mirrors in the median ocellus of Archaeognatha. It is important to broaden the study of crystallization strategies to obtain organic crystals with purine rings in the laboratory. In this work, a facile one-step synthesis route to fabricate bio-inspired xanthine crystals is reported for the first time. The obtained rhomboidal xanthine nanoplates have similar morphology and size to biogenic xanthine crystals. Their length and thickness are about 2–4 μm and 50 nm, respectively. Lattice parameters, crystal structure, formation mechanism and optical properties of synthetic single-crystalline xanthine nanoplates were investigated in detail in this work. The obtained xanthine nanoplate crystals are proposed to be anhydrous xanthine with monoclinic symmetry, and the xanthine nanoplates mainly expose the (100) plane. It is proposed that the anhydrous xanthine nanoplates are formed via an amorphous xanthine intermediate precursor. The synthetic anhydrous xanthine nanoplates exhibit excellent optical properties, including high diffuse reflectivity, strong depolarization and pearlescent luster. This work provides a new design to synthesize bio-inspired organic molecular crystals with excellent optical properties.
研究领域:
晶体学
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生物材料
·
光学
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产品关联
DOI
PubMed
高影响因子
Materials Today Communications
·
2024
·
IF 3.7
Yicheng Zhou,Li Dong,Jiazheng Zhang,Rongrong Qiao,Lin Liu,Chao Liu,Xiaobo Zhang,Zhiwei Tong
In this study, a nanocomposite of Polyaniline/titanium carbide (PANI/Ti 3 C 2 ) was synthesized through self-assembly and in situ polymerization for use as a sensor material to simultaneously detect guanine (G), adenine (A), and uric acid (UA). X-ray diffraction (XRD), Fourier transform infrared spectrometer (FTIR), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and X-ray photoelectron spectroscopy (XPS) were utilized to confirm the successful synthesis of PANI/Ti 3 C 2 . Electrochemical analysis methods including differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) were used to detect the electrochemical performance of PANI/Ti 3 C 2 modified glassy carbon electrode (PANI/Ti 3 C 2 /GCE). The pH dependence, electrochemically active surface area (ECSA), electron transport , stability, reproducibility, sensitivity, and selectivity of the modified GCE were examined. Compared to previous reports, the nanocomposite exhibited excellent performance. Uric acid, guanine, and adenine detection limits were 0.33, 0.31, and 0.28 μM, respectively, over concentration ranges of 0.62 - 20.10, 0.31 - 17.00, and 0.31 - 17.00 μM. The nanocomposite demonstrated high recovery and low error in serum sample detection. In summary, PANI/Ti 3 C 2 is a sensor material with excellent electrochemical properties .
研究领域:
材料科学
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分析化学
·
生物医学工程
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产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2010
·
IF 2.338
Tao Liu,Xiangbin Zhu,Lin Cui,Peng Ju,Xiangjin Qu,Shiyun Ai
A novel and reliable electrochemical sensor based on PbO 2 -carbon nanotubes-room temperature ionic liquid (i.e., 1-butyl-3-methylimidazolium hexafluorophosphate, BMIMPF6) composite film modified glassy carbon electrode (GCE) (PbO 2 –MWNT–RTIL/GCE) was proposed for simultaneous and individual determination of guanine and adenine . The guanine and adenine oxidation responses were monitored by differential pulse voltammetric (DPV) measurement. Compared with the bare electrode, the PbO 2 –MWNT–RTIL/GCE not only significantly enhanced the oxidation peak currents of guanine and adenine, but also lowered their oxidation overpotentials, suggesting that the synergistic effect of PbO 2 , MWNT and RTIL could dramatically improve the determining sensitivity of guanine and adenine. The PbO 2 –MWNT–RTIL/GCE showed good stability, high accumulation efficiency and enhanced electrocatalytic ability for the detection of guanine and adenine. Besides, the modified electrode also exhibited good behaviors in the simultaneous detection of adenine and guanine with the peak separation of 0.29 V in 0.1 M pH 7.0 phosphate buffer solution (PBS). Under the optimal conditions, the detection limit for individual determination of guanine and adenine was 6.0 × 10 −9 M and 3.0 × 10 −8 M ( S / N = 3), respectively. The proposed method for the measurements of guanine and adenine in herring sperm DNA was successfully applied with satisfactory results.
研究领域:
生物化学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
Drug Metabolism and Pharmacokinetics
·
2022
·
IF 2.041
Shanshan Wang,Yan Qin,Qiuyu Wen,Quan Xia,Ruoyu Gu,Sheng Wang,GuanJun Chen,Chao Tan,Chenlin Shen,Shuai Song
Adverse reactions to azathioprine (AZA) vary greatly among individuals, which is associated with the variable levels of its major metabolites 6-thioguanine nucleotides (6-TGN) and 6-methylmercaptopurine (6-MMP). The intestinal microbiota has been proven to contain AZA-metabolizing enzymes, although the explicit role of the intestinal microbiota in AZA metabolism in vivo remains poorly comprehended. In this study, the pharmacokinetic behaviours of 6-TGN and 6-MMP were assessed in the pseudo germ-free (PGF) group and control group following oral administration of AZA. The AUC 0-t and C max of 6-TGN in the PGF group were significantly decreased by 34.0% and 35.0% ( P < 0.05) compared with those in the control group. Additionally, the AUC 0-t and C max of 6-MMP were reduced by 27.9% and 34.2% in the PGF group, although the differences were not significant. The TPMT and NUDT15 genotypes of rats in the two groups were genetically identical. The expression levels of key AZA-metabolizing enzymes in liver were not different between two groups. Furthermore, the major metabolites of AZA in the incubation system with intestinal microbial enzymes were identified. In summary, shifts in the composition of the intestinal microbiota may regulate the exposure of 6-TGN in vivo by altering the gut microbial metabolism of AZA.
研究领域:
药理学
·
微生物学
·
药物基因组学
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产品关联
DOI
PubMed
高影响因子
ELECTROANALYSIS
·
2018
·Vol. 30
·期 5
·842-851
·
IF 2.851
Meiling Wang,Mingzhu Cui,Weifeng Liu,Xuguang Liu,Bingshe Xu
Here, a facile reduction approach for synthesis of β-cyclodextrin functionalized reduced graphite oxide with the aid of ionic liquid (βCD/IRGO) was reported. This strategy relies on the simple mixing of pre-pared aqueous βCD/GO with ionic liquid (IL) at high temperatures, which can bring about a well dispersed βCD/RGO in the utilized IL (βCD/IRGO) with a synchronous evaporation of water. The as-prepared βCD/IRGO product can be stably re-dispersed in water (up to 1.0 mg/mL) after washed and dried. And the presented βCD/IRGO composites possess high supramolecular recognition capability and good conductivity for realizing enhanced electrochemical response towards simultaneous detection of guanine and adenine compared with other βCD/carbon (RGO or carbon nanotube) based composites. In the presence of one of analysts, the linear response ranges is 0.03–10.0 μM for guanine and 0.02–7.0 μM for adenine, respectively, with detection limit of 0.01 μM for both guanine and adenine (S/N=3). This ease of making the βCD/IRGO composite will open up possibilities of its diversifier applications.
研究领域:
材料科学
·
电化学分析
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）