AGRICULTURE ECOSYSTEMS & ENVIRONMENT
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2017
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IF 4.099
Congyan Wang,Jiawei Zhou,Jun Liu,Kun Jiang,Daolin Du
Soil N-fixing bacteria communities (SNB) can increase soil N availability, which can facilitate invasions of many kinds of invasive plant species. Meanwhile, variability in the forms of anthropogenic N deposition can mediate obvious shifts in both soil physicochemical properties and soil microbial communities (especially SNB). This can alter the soil micro-ecological mechanisms of plant invasions via changes in soil characteristics and soil microbial communities, especially SNB. This study uses next-generation high throughput sequencing technology to provide insight into the effects of invasion by Amaranthus retroflexus L. on the structure and diversity of SNB under different forms of simulated N deposition (SND). Soil pH under A. retroflexus invasion under various forms of SND was 8.08% lower than controls under a NH 4 -N treatment ( P < 0.05), 15.15% lower than controls under a NO 3 -N treatment ( P < 0.05), and 6.57% lower than controls under CO(NH 2 ) 2 -N and Mix-N treatments ( P < 0.05). Meanwhile, soil pH was one of the most important environmental variables explaining changes in the structure and diversity of SNB. The total number of species of SNB under A. retroflexus invasion was approximately 13.13% higher than controls for the NH 4 -N treatment ( P > 0.05), 11.00% higher than controls for the NO 3 -N treatment ( P > 0.05), 39.42% higher than controls for the CO(NH 2 ) 2 -N treatment ( P < 0.05), and 27.67 higher than controls for the Mix-N treatment ( P < 0.05). The positive effects mediated by the combined treatments of A. retroflexus invasion and SND may be ascribed to enhanced soil nutrient content (especially N), which can enhance the abundance of the nifH gene. The effects of A. retroflexus invasion on the total number of species of SNB under organic and mixed-N addition were greater than under inorganic N addition. This may be a consequence of a disturbance of the balance of inorganic to organic N for SND, as a result of the addition of a single form of inorganic N, which can diminish the stimulatory effects of soil nutrient content on SNB under inorganic N. Another potential cause of this pattern may be stronger acidification under inorganic N addition, which can exert more pronounced effects on SNB. LEfSe analysis showed that some species changed significantly under A. retroflexus invasion in the presence of SND (i.e., Rhodobacteraceae under NH 4 -N and Proteobacteria under CO(NH 2 ) 2 -N). Thus, A. retroflexus invasion in the presence of SND caused obvious shifts in certain species of SNB and increased the total number of species of SNB in particular, to facilitate its further invasion.
研究领域:
生态学
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微生物学
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2021
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IF 10.588
Mengqi Zheng,Hongjun Han,Jingxin Shi,Zhengwen Zhang,Wencheng Ma,Chunyan Xu
In current study, nano-Fe 3 O 4 @activated coke enhanced bio-system (FEBS) under limited-oxygen condition was applied for efficient treatment of aromatic organics in coal pyrolysis wastewater. Metagenomic analyses revealed functional microbiome linkages and mechanism involved in aromatic ring-cleavage. Based on biodegradation efficiency in different reactors, FEBS supplementation conferred the best organic removal (avg. 92.29%). It also showed a remarkable advantage in biodegradability maintenance (> 40%) over control reactors. Metagenomics profiling revealed the degradation processes were driven by Fe 3 O 4 redox reactions and microbial biofilm, while the suspended sludge was the principal force for aromatic mineralization . Based on the analysis of functional species and genes, most bacteria cleaved the benzene ring preferably through the aerobic pathways, mediated by catechol 1, 2-dioxygenase, catechol 2, 3-dioxygenase and protocatechuate 3, 4-dioxygenase (66−84%). Ecological network showed that Comamonas testosterone -centered microbiome and Azotobacter linked to the nitrogen (N)-heterocyclic ring-cleavage. Network linkage further demonstrated that Alicycliphilus and Acidovorax were the key tone taxa involved in benzene ring-cleavage. Finally, combined with analysis of degradation products , bacteria degraded N-heterocyclic ring containing organic aromatic compounds (quinoline) mainly through anaerobic processes , whereas cleavage of benzene ring preferred aerobic pathways. The enriched functional species were the primary reason for the enhanced biodegradation in FEBS.
研究领域:
环境工程
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微生物学
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宏基因组学
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废水处理
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DOI
PubMed
高影响因子
Journal of Water Process Engineering
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2022
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IF 7.34
Qiaona Xie,Yaquan Zhang,Haifeng Zhuang,Ruting Liang,Xiaoying Zhan,Yufei Zhao,Shengdao Shan,Hao Wu
In order to strengthen the treatment of phenolic wastewater by anaerobic biotechnology, micron-grade high iron-containing fly ash/loofah sponge biochar (MHIFA/LsBC) was prepared and added to anaerobic activated sludge . It was domesticated and cultured in anaerobic bottle by a constant temperature oscillation incubator. Its promoting effect on the anaerobic degradation of high-concentrated 2,4,6-trichlorophenol (2,4,6-TCP) wastewater and its effect on the composition and function of microbial community were studied. The results indicated that with MHIFA/LsBC, the final removal rates of COD and 2,4,6-TCP in R1 (75.6% and 75.1%) were 2.1 and 1.81 times than those of the control group, respectively. The maximum methane yield was 107.25 mL/d. MHIFA/LsBC addition resulted in higher biomass and conductivity, and simultaneously increased the concentration of EPS and coenzyme F 420 . Microbial community analysis indicated that the enriched electrogenic bacteria Pseudomonas might perform DIET with Methanothrix through bioelectrical connection assisted by MHIFA/LsBC, and promote the anaerobic degradation of 2,4,6-TCP. As the functional genus induced by MHIFA/LsBC, Pseudomonas promoted the degradation and transformation of phenolic pollutants, and Azotobacter played a keystone role in metabolism for hydrogen production by preserving the NADH/NAD + balance.
研究领域:
环境工程
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微生物学
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生物技术
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废水处理
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DOI
高影响因子
Frontiers in Microbiology
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2025
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IF 4.5
Xuan Bo,Yong Liu,Hong Zhang,Chao Su,Yang Miao
Spent mushroom substrate (SMS) is a promising organic amendment for remediating degraded soils, yet its effectiveness is often limited by poor compost maturity and stability. This study aimed to enhance the quality and functionality of SMS compost through microbial inoculation and to evaluate its impact on soil improvement and plant growth. Three plant growth-promoting strains [Bacillus subtilis (B. subtilis), Azotobacter chroococcum (A. chroococcum), and Paenibacillus mucilaginosus (P. mucilaginosus)] and their combination as a mixed microbial inoculant (MMI) were used in composting. Subsequent pot experiments assessed changes in soil physicochemical properties, nutrient levels, microbial diversity, and the growth of Pinus sylvestris seedlings. Results showed that SMS amended with MMI significantly improved soil porosity, pH, and nutrient content, while enriching beneficial microbial communities dominated by Proteobacteria and Basidiomycota. Moreover, MMI treatment notably enhanced plant height, stem diameter, and chlorophyll content compared to control treatments. These findings highlight the synergistic effect of mixed microbial inoculants in optimizing SMS compost and promoting soil and plant health. The approach offers a sustainable strategy for the circular reuse of agricultural waste and effective restoration of degraded soils.
研究领域:
土壤科学
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微生物学
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植物学
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农业可持续性
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环境修复
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DOI
PubMed
高影响因子
ISME Journal
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2023
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IF 11
Liu Xiaohan,Li Ping,Wang Helin,Han Li-Li,Yang Kai,Wang Yanhong,Jiang Zhou,Cui Li,Kao Shuh-Ji
Biological nitrogen fixation (BNF), the conversion of N 2 into bioavailable nitrogen (N), is the main process for replenishing N loss in the biosphere. However, BNF in groundwater systems remains poorly understood. In this study, we examined the activity, abundance, and community composition of diazotrophs in groundwater in the Hetao Plain of Inner Mongolia using 15 N tracing methods, reverse transcription qPCR (RT-qPCR), and metagenomic/metatranscriptomic analyses. 15 N 2 tracing incubation of near in situ groundwater (9.5–585.4 nmol N L −1 h −1 ) and N 2 -fixer enrichment and isolates (13.2–1728.4 nmol N g −1 h −1 , as directly verified by single-cell resonance Raman spectroscopy), suggested that BNF is a non-negligible source of N in groundwater in this region. The expression of nifH genes ranged from 3.4 × 10 3 to 1.2 × 10 6 copies L −1 and was tightly correlated with dissolved oxygen (DO), Fe(II), and NH 4 + . Diazotrophs in groundwater were chiefly aerobes or facultative anaerobes, dominated by Stutzerimonas , Pseudomonas , Paraburkholderia , Klebsiella , Rhodopseudomonas , Azoarcus , and additional uncultured populations. Active diazotrophs, which prefer reducing conditions, were more metabolically diverse and potentially associated with nitrification, sulfur/arsenic mobilization, Fe(II) transport, and CH 4 oxidation. Our results highlight the importance of diazotrophs in subsurface geochemical cycles.
研究领域:
环境微生物学
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地球化学
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水文地质学
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微生物生态学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）