Membranes
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2025
·Vol. 15
·期 5
·141
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IF 3.6
Ning Li,Hongbo Xin,Keyu Deng
Outer membrane vesicles (OMVs) are extracellular vesicles secreted by Gram-negative bacteria with diameters of 20–250 nm. OMVs contain various biologically active substances from their parent bacteria, such as proteins, lipids, and nucleic acids.Escherichia coliNissle 1917 (EcN) is a Gram-negative probiotic that resides in the human intestine. EcN-derived OMVs are pivotal in modulating intestinal immune responses. However, few studies have addressed the heterogeneity of EcN-derived OMVs in terms of size, significantly limiting the research on their clinical applications. Currently, there are a lack of feasible methods for obtaining EcN-derived OMVs of different sizes. To address this knowledge gap, we developed a membrane filtration method to isolate EcN-derived OMVs of varying sizes. In this study, we first used gradient filtration to isolate high-purity EcN-derived OMVs and conducted a proteomic analysis. Subsequently, we used membrane filtration to separate the EcN-derived OMVs by size. We successfully obtained EcN-derived OMVs of three specific sizes: <50 nm, 50–100 nm, and 100–300 nm. We then performed proteomic analyses of these EcN-derived OMVs and compared their protein profiles. Finally, we compared the ability of each EcN-derived OMV type to induce RAW264.7 macrophages to secrete the pro-inflammatory factor interleukin (IL)-1β and the anti-inflammatory factor IL-10. The EcN-derived OMVs contained 646 different proteins overall; those of different sizes contained different protein types. Among them, the EcN-derived OMVs in the <50 nm group contained significantly fewer proteins (262 different types in total) than those in the 50–100 nm (1603 types) and 100–300 nm (1568 types) groups. Furthermore, the <50 nm group had fewer membrane proteins (40) than the 50–100 nm (215) and 100–300 nm (209) groups. We also found that RAW264.7 macrophages secreted different concentrations of IL-1β and IL-10 following co-incubation with the three EcN-derived OMV types. The 50–100 nm EcN-derived OMV group showed a stronger effect in terms of inducing inflammatory cytokine secretion compared to the other two groups. This study provides direct experimental evidence that EcN-derived OMVs of different sizes exhibit heterogeneous properties.
研究领域:
微生物学
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免疫学
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蛋白质组学
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产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
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2025
·
IF 19
Lujiao Wang,Song Yang,Tao Zhou,Siyu Yang,Pengyu Bi,Jiangyi Zhou,Qi Wang,Hai Qiao
Extracellular vesicles, such as Escherichia coli outer membrane vesicles (OMVs), have been widely explored as carriers for contrast agents, yet their application in ultrasound imaging remains limited. In this study, heat-responsive nB-OMVs are developed by loading n-butanol into OMVs (nB-OMVs). Upon heating, the decreased solubility of n-butanol triggered phase separation within the vesicles, resulting in enhanced ultrasound signals, including fundamental, harmonic, and subharmonic components. Experimental results demonstrated that nB-OMVs retained the native morphology of OMVs, along with their tumor-targeting accumulation and tumor growth inhibition capabilities. nB-OMVs injection induces red blood cell extravasation into tumors, under near-infrared (NIR) irradiation, these extravasated RBCs acted as photothermal mediators, generating a localized thermal environment that activated intratumoral nB-OMVs and further amplified the ultrasound signal. In B-mode and contrast-enhanced ultrasound (CEUS) imaging, tumor grayscale intensities increased by 4.6- and 8.5-fold, respectively. This work provides a promising strategy for ultrasound-based tumor imaging using extracellular vesicles.
研究领域:
医学影像学
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生物材料学
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肿瘤学
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产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
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IF 19
Donglin Cai,Zhelun Li,Wendong Gao,Yuqing Mu,Jiaying Liu,Yufeng Zhang,Xiaohan Mei,Jingjing Quan,Lan Xiao,Yin Xiao
The temporary transition of macrophages from a pro-inflammatory macrophages (M1) to an anti-inflammatory macrophages (M2) is crucial for tissue repair and regeneration processes. Selectively targeting M1 macrophages is preferred to modulate inflammatory responses. However, macrophage populations are inherently heterogeneous, and the competitive phagocytosis of nanoparticles (NPs) by these two subtypes has not been thoroughly investigated. This study establishes a 3D cell co-culture system to evaluate the competitive phagocytosis of M1-targeting NPs under dynamic fluidic flow conditions. Bacterial outer membrane vesicles (OMVs) are utilized as a “trojan horse” to decorate gold nanocage (AuNC), creating hybrid NPs (AuNC-OM) capable of selectively targeting M1 macrophages. It is noted that the selective uptake of AuNC-OM by M1 macrophages is mediated through phagocytosis-related receptors CD64 and CD14, which are highly expressed on the M1 macrophage surfaces. Furthermore, the study demonstrates that AuNC-OM selectively targets M1 macrophages without affecting the phagocytosis of M2 macrophages. Once internalized, the nanoparticles release anti-inflammatory drugs, effectively reversing the inflammation. The release of dexmedetomidine from AuNC-OM protects against lipopolysaccharide (LPS)-induced bone resorption in a mouse model. This study presents a novel system for accurately assessing the M1 macrophage-targeting capabilities and introduces an innovative strategy for M1 macrophage-targeted therapy.
研究领域:
免疫学
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纳米医学
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药理学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
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2025
·
IF 8.2
Han Yin,Jingqi Zhao,Hanyu Zhong,Nanxi Zhu,Yiyi Zhang,Jiyao Li,Jun Luo,Kunneng Liang,Bo Han,Jiaojiao Yang
Current treatments for skin infections employing single-agent antimicrobials have demonstrated pronounced limitations. Here, we have engineered an efficient antibacterial composite consisting of MXene and poly(vinyl alcohol) electrospun fibrous membranes, which are further functionalized with outer membrane vesicles (OMVs) using a tannic acid (TA) bridge. Upon near-infrared irradiation, OMVs augment the photothermal properties of MXene due to their “bedquilt effect”. Meanwhile, the temperature rise facilitates active antibacterial substance release by increasing the permeability of the OMVs. The synergistically enhanced antimicrobial ability of the OMVs and photothermal materials achieves a potentiated antibacterial effect, resulting in a near 100% antibacterial efficacy in vitro and in vivo. This multifunctional, antibiotic-free dressing membrane, which integrates photothermal and biological antibacterial properties, presents a promising therapeutic strategy for the management of skin wound infections.
研究领域:
材料科学
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医学
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生物工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MICROENCAPSULATION
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2020
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IF 2.287
Jinyan Shi,Zhiwei Ma,Hao Pan,Yang Liu,Yuqi Chu,Jinglei Wang,Lijiang Chen
Aim In this study, 5-fluorouracil (5-FU) is delivered to target colon without the interference of mononuclear phagocyte system (MPS).Methods Outer membrane vesicles (OMVs) were used as the biological shield to disguise mesoporous silica (MSN) and 5-FU. OMVs-MSN-5-FU were prepared by high pressure co-extrusion, and characterised on the basis of size, drug loading, transmission electron microscope, infra-red spectroscopy, differential scanning calorimetry, thermal gravity analysis, % in vitro release, MTT assay, cell uptake and in vivo imaging.Results OMVs-MSN-5-FU with −18.22 ± 0.17 mV zeta potential and 90.4 ± 9.1 nm size were used for oral treatment of colon cancer. Drug loading of the drug was 50.22%±0.17 (w/w). The cumulative release of OMVs-MSN-5-FU reached 75.07%±0.94 in tumour microenvironment. The percentage of cell viability of OMVs-MSN-5-FU was 33.75%±2.73. In vivo experiments results confirmed that OMVs-MSN-5-FU could be taken up by colon cancer cells.Conclusions The study provided a promising nano platform for the targeting treatment of colon cancer.
研究领域:
药理学
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纳米医学
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肿瘤学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Pharmacology
·
2025
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IF 4.8
Wen-Ba Wang,Jia-Yang Wan,Dao-Jun Yu,Hai-Xia Du,Hui-Fen Zhou,Hai-Tong Wan,Jie-Hong Yang
Introduction: Carbapenem-resistant Klebsiella pneumoniae (CRKp) infection poses a significant global public health challenge, with the misuse of antibiotics further contributing to the development of resistance and triggering harmful inflammatory responses. Outer membrane vesicles (OMVs) released by CRKp under sub-lethal concentration of MEM pressure (KOMV-MEM) exhibit enhanced virulence and greater efficiency in transferring resistance genes.Methods: We investigated the inhibitory effects of chlorogenic acid (CA) on KOMV-MEM characteristics and its protective role in KOMV-MEM infected mice. Based on LC-MS proteomic analysis of vesicles, we screened for potential targets of KOMV-MEM in promoting macrophage (MØ) pyroptosis pathways and inducing resistance gene transfer. Subsequently, computational predictions and experimental validation were performed to determine how CA regulates these mechanisms.Results: This study confirmed that, under MEM pressure, the exacerbated infection levels in CRKp-inoculated mice are attributable to the high virulence of KOMV-MEM. Computational and experimental results demonstrated that CA inhibits pyroptosis by reducing MØ capture of KOMV-MEM through blocking the interaction between GroEL and LOX-1. Furthermore, CA prevents the spread of resistance genes by disrupting the conjugation and transfer processes between KOMV-MEM and recipient bacteria. Finally, in vitro and in vivo assays showed that CA inhibits KOMV-MEM resistance enzymes, thereby preventing the hydrolysis of MEM in the environment and depriving susceptible bacteria of protection.Discussion: These findings provide the first confirmation that CA can inhibit both the virulence and the transmission of drug resistance in KOMV-MEM. This underscores the potential of CA treatment as a promising antimicrobial strategy against CRKp infection.
研究领域:
微生物学
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药理学
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感染与免疫
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
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2021
·
IF 9.028
Sheng-Song Yu,Jie-Jie Chen,Rui-Fen Cheng,Yuan Min,Han-Qing Yu
The biogeochemical cycle of iron is of great importance to living organisms on Earth, and dissimilatory metal-reducing bacteria (DMRB) with the capability of reducing hematite (α-Fe2O3) by outer-membrane (OM) cytochromes play a great role in the iron cycle. However, the dynamic binding of cytochromes to α-Fe2O3 at the molecular level and the resulting impact on the photon-to-electron conversion of α-Fe2O3 for the iron cycle are not fully understood. To address these issues, two-dimensional IR correlation analysis coupled with molecular dynamics (MD) simulations was conducted for an OmcA–Fe2O3 system as OmcA bonds stronger with hematite in a typical DMRB,Shewanella. The photoelectric response of α-Fe2O3 with the OmcA coating was evaluated at three different potentials. Specifically, the binding groups from OmcA to α-Fe2O3 were in the sequence of carboxyl groups, amide II, and amide I. Further MD analysis reveals that both electrostatic interactions and hydrogen bonds played essential roles in the binding process, leading to the structural changes of OmcA to facilitate iron reduction. Moreover, the OmcA coating could store the photogenerated electrons from α-Fe2O3 like a capacitor and utilize the stored electrons for α-Fe2O3 reduction in dark and anoxic environments, further driving the biogeochemical cycle of iron. These investigations give the dynamic information on the OM protein/hematite interaction and provide fundamental insights into the biogeochemical cycle of iron by taking the photon-induced redox chemistry of iron oxide into consideration.
研究领域:
微生物学
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生物地球化学
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环境科学
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DOI
高影响因子
Membranes
·
2023
·Vol. 13
·期 7
·665
·
IF 4.2
Jie Li,Ting He,Hongyu Chen,Yangming Cheng,Enrico Drioli,Zhaohui Wang,Zhaoliang Cui
HighlightsPreparation of anti-wetting polypropylene membrane using environmentally friendly diluents via TIPS methodOptimization of Preparation Process for Polypropylene Hollow Fiber MembraneFluorinated polymer modified membrane enhances anti-wetting performance and blood compatibilityAbstractA high-performance polypropylene hollow fiber membrane (PP-HFM) was prepared by using a binary environmentally friendly solvent of polypropylene as the raw material, adopting the thermally induced phase separation (TIPS) method, and adjusting the raw material ratio. The binary diluents were soybean oil (SO) and acetyl tributyl citrate (ATBC). The suitable SO/ATBC ratio of 7/3 was based on the size change of the L-L phase separation region in PP-SO/ATBC thermodynamic phase diagram. Through the characterization and comparison of the basic performance of PP-HFMs, it was found that with the increase of the diluent content in the raw materials, the micropores of outer surface of the PP-HFM became larger, and the cross section showed a sponge-like pore structure. The fluoropolymer, Hyflon ADx, was deposited on the outer surface of the hollow fiber membrane using a physical modification method of solution dipping. After modification, the surface pore size of the Hyflon AD40L modified membranes decreased; the contact angle increased to around 107°; the surface energy decreased to 17 mN·m−1; and the surface roughness decreased to 17 nm. Hyflon AD40L/PP-HFMs also had more water resistance properties from the variation of wetting curve. For biocompatibility of the membrane, the adsorption capacity of the modified PP membrane for albumin decreased from approximately 1.2 mg·cm−2to 1.0 mg·cm−2, and the adsorption of platelets decreased under fluorescence microscopy. The decrease in blood cells and protein adsorption in the blood prolonged the clotting time. In addition, the hemolysis rate of modified PP membrane was reduced to within the standard of 5%, and the cell survival rate of its precipitate was above 100%, which also indicated the excellent biocompatibility of fluoropolymer modified membrane. The improvement of hydrophobicity and blood compatibility makes Hyflon AD/PP-HFMs have the potential for application in membrane oxygenators.Keywords:polypropylene;thermally induced phase separation;environmentally friendly diluent;fluoropolymer;ECMO
研究领域:
材料科学
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生物医学工程
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膜技术
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产品关联
DOI
PubMed
高影响因子
Science Advances
·
2024
·Vol. 10
·期 12
·
IF 11.7
Xianyuan Wei,Bin Xue,Shuangchen Ruan,Jintong Guo,Yujing Huang,Xiaorui Geng,Dan Wang,Cangtao Zhou,Jun Zheng,Zhen Yuan
To eliminate multidrug-resistant bacteria of Acinetobacter baumannii, we screened 1100 Food and Drug Administration–approved small molecule drugs and accessed the broxyquinoline (Bq) efficacy in combination with various metal ions. Antibacterial tests demonstrated that the prepared Zn(Bq)2 complex showed ultralow minimum inhibitory concentration of ~0.21 micrograms per milliliter with no resistance after 30 passages. We then constructed the nano zeolitic imidazolate framework-8 (ZIF-8) as a drug carrier of Zn(Bq)2 and also incorporated the photosensitizer chlorin e6 (Ce6) to trace and boost the antibacterial effect. To further ensure the stable and targeted delivery, we genetically engineered outer membrane vesicles (OMVs) with the ability to selectively target A. baumannii. By coating the ZnBq/Ce6@ZIF-8 core with these OMV, the resulted drug (ZnBq/Ce6@ZIF-8@OMV) exhibited exceptional killing efficacy (>99.9999999%) of A. baumannii. In addition, in vitro and in vivo tests were also respectively carried out to inspect the remarkable efficacy of this previously unknown nanodrug in eradicating A. baumannii infections, including biofilms and meningitis.
研究领域:
微生物学
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药理学
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纳米医学
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产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-14
·
IF 15.7
Gong Xu,Liu Shan,Xia Bozhang,Wan Yichen,Zhang Shuyi,Zhang Baoyan,Wang Zehao,Chen Junge,Xiao Fei,Liang Xing-Jie,Yang Yun
Genetically engineered commensal bacteria are promising living drugs, however, their therapeutic molecules are frequently confined to their colonization sites. Herein, we report an oral protein delivery technology utilizing an engineered bacterial type zero secretion system (T0SS) via outer membrane vesicles (OMVs). We find that OMVs produced in situ by Escherichia coli Nissle 1917 (EcN) can penetrate the intact gut epithelial barrier to enter the circulation and that epithelial transcytosis involves pinocytosis and dynamin-dependent pathways. EcN is engineered to endogenously load various enzymes into OMVs, and the secreted enzyme-loaded OMVs are able to stably catalyze diverse detoxification reactions against digestive fluid and even enter the circulation. Using hyperuricemic mice and uricase delivery as a demonstration, we demonstrate that the therapeutic efficacy of our engineered EcN with a modified T0SS outperforms that with a direct protein secretion apparatus. The enzyme-loaded OMVs also effectively detoxify human serum samples, highlighting the potential for the clinical treatment of metabolic disorders.
研究领域:
分子生物学
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医学工程
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微生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2022
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IF 14.224
Xiaofeng Yi,Shurui Liu,Mingyu Luo,Qingbiao Li,Yuanpeng Wang
Microbe-photocatalyst biohybrids, integrating the optimal attributes of whole-cell catalysts and nanometer photocatalysts, have emerged as a promising strategy for environment-associated applications. However, few such biohybrids have been tested for complex pollution systems. Herein, we constructed an outer membrane photosensitized Geobacter sulfurreducens ( G. sulfurreducens )-CdS biohybrid, which enabled to generate stronger photocurrent in response to irradiation and meanwhile achieved an significant promotion for the redox transformation of Cr(VI) and tetracycline compared with that of bare G. sulfurreducens or CdS counterparts. Further analysis revealed that the outer membrane played a significant role in photoelectron transfer. Differential pulse voltammetry (DPV) tests demonstrated that CdS enhanced the catalytic activity of C-type cytochromes on the outer membrane under irradiation, resulting in the increase of electron-hole pairs separation efficiency. The possible degradation pathway of tetracycline was proposed based on determined intermediates, whose toxicities were well evaluated. Importantly, the toxicity of the final detected intermediates was apparently decreased. Overall, this work aims to explore the working mechanisms of the novel G. sulfurreducens -CdS biohybrid system and opens up a new avenue to purifying combined wastewater by microbe-photocatalyst biohybrids.
研究领域:
环境科学
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微生物学
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2025
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IF 5.6
Yue Huang,Lunhao Zhi,Chong Cheng,Qiang Wei,Shudong Sun,Changsheng Zhao
In extracorporeal membrane oxygenation (ECMO) treatment, designing membrane with self-anticoagulant properties can solve problems resulting from the adverse effect of anticoagulants. In this study, 2,4,6-Triformylphloroglucinol (Tp) and 2,5-Diaminobenzenesulfonic acid (Pa-SO 3 H) were applied to grow a sulfonated COF film in situ on the polyethersulfone (PES) membrane. The introduction of sulfonic groups increased the hydrophilicity and electronegativity of the TpPa COF film, improved its anti-protein adhesion properties, maintained the normal morphology of blood cells, and endowed the COF film with antithrombotic properties. In the porcine blood circulation test, the duration to increase So 2 (O 2 saturation) from ∼75–95 % in TpPa-SO 3 H COF/PES membrane (M-TpPa-SO 3 H) was 70 min shorter than that in TpPa COF/PES membrane (M-TpPa). This preparation method is applicable to the preparation of not only flat membranes, but also hollow fiber membranes. These findings emphasize the potential of M-TpPa-SO 3 H in ECMO applications, providing superior antithrombotic property and CO 2 efflux potential.
研究领域:
生物医学工程
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材料科学
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血液学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）