Foods
·
2023
·Vol. 12
·期 18
·3467
·
IF 5.2
Weiqing Lan,Bingjie Zhang,Jintao Du,Shengyun Zhu,Xiao Xu,Jing Xie
The effects of allicin and antioxidant of bamboo leaves (AOB) on the quality of bullfrogs (Lithobates catesbeiana) during refrigerated storage (4 °C) were investigated. The quality changes in samples treated with deionized water (CK), allicin solution (All), antioxidant of bamboo leaves (AOB), and allicin solution combined with AOB solution (AA) in microbiological, physicochemical, and sensory evaluation were analyzed, respectively. The results demonstrated that combination treatment inhibited the increase in total viable counts, delayed the decrease in amino acid content, and retarded the sensory deterioration. Preservative treatment has an inhibitory effect on the early storage of PBC, which can reduce PBC by about 1.0 log CFU/g. The reduction in thiobarbituric acid (TBA) content and total volatile basic nitrogen (TVB-N) content indicated that combination treatment could better restrain the lipid oxidation and degradation of protein than the CK group and single-treatment group. In addition, the TVB-N content in the AA group still did not exceed the threshold on the 14th day. As a consequence, combination treatment prolonged the shelf life of bullfrogs for another six days. Therefore, allicin and AOB with excellent antioxidant and antimicrobial activity could be an effective approach to delay the biochemical reaction of refrigerated bullfrogs. This study has provided a potential approach for increasing the shelf life of bullfrogs and preserving their quality during refrigerated storage.
研究领域:
食品科学
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微生物学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2025
·
IF 5.5
Quan Xu,Jinmei Wu,Yulan Zhao,Zhipeng Zhou,Yi Liu,Zhiyong Song,Longhao Chen,Heyou Han
Drug-resistant bacterial pneumonia presents a substantial challenge for targeted drug delivery to the infected lung site due to the existence of mucus and gas-blood barriers. Allicin, renowned for its extensive bioactivity, holds promise as an antimicrobial agent. However, its unregulated bioavailability restricts both its antibacterial and anti-inflammatory efficacy. As a result, precise targeting of the infection site for controlled drug release is crucial in the treatment of drug-resistant bacterial pneumonia. In this study, we developed a macrophage cell membrane-modified nanoallicin composite material (Allicin@MSN@CM) by utilizing cell membrane modification technology. By harnessing the homing ability of macrophage cell membranes to inflammatory sites, we enabled the active aggregation of nanomaterials during circulation within the body. Furthermore, α-hemolysin secreted by MRSA bacteria was employed to perforate the cell membrane, facilitating on-demand drug release at the infection site. In vitro antibacterial experiments demonstrated that the material possesses strong antibacterial properties, alongside good stability and biocompatibility. Additionally, in a mouse model of lung infection, it not only effectively eradicated bacteria but also reduced lung inflammation. This study provides valuable insights into the application of nanocarriers in delivering traditional Chinese medicine to address the challenges posed by bacterial drug-resistant pneumonia.
研究领域:
医学
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纳米技术
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药物递送
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产品关联
DOI
高影响因子
Materials Science and Engineering B-Advanced Functional Solid-State Materials
·
2025
·
IF 4.6
Yan Li,Ming Shen,Jing Kong
Although the antibacterial properties of ZnO nanoparticles have been widely studied for potential biomedical applications, their relatively mild antibacterial activity still limits the clinical application of ZnO-based antibacterial materials. Here, we reported a new method to improve the antibacterial activity and visible light absorption of ZnO nanoparticles by incorporating allicin on the surface. The synthesis was performed through a simple condensation reflux reaction to obtain the designed series of ZnO/Allicin composites. FT-IR, XRD, and TEM analysis proved the structure and composition of the obtained composites. All composites show visible light absorption in the UV–visible diffuse reflectance spectroscopy analysis. Through antibacterial activity tests, the ZnO/allincin-1 % sample showed significantly enhanced activity (2-fold increase) against Staphylococcus aureus after visible light excitation. Antibacterial mechanistic investigations reveal that the composition of allicin with the ZnO nanoparticles not only improves ROS productive ability but also enhances the release of Zn 2+ . Overall, this study provides a practical, convenient, and economic strategy to refine the visible light absorption and antibacterial activity of ZnO without introducing more toxic metal ions. In the meantime, this new nanomaterial could be a good starting point for initiating clinically applicable ZnO nanocomposites.
研究领域:
材料科学
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微生物学
·
生物医学工程
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Yongxu Liu,Rong Song,Xiaohong Zhang,Dawei Zhang
Herein, the natural extract of garlic, allicin (Alli), was added into chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide (GO) composites to develop the nanofibrous membranes with strong antibacterial activity and sustained-release properties by electrospinning technology. Vitro Alli release test showed that the release rate and amount of Alli could be regulated by the content of GO in the nanofibrous membrane. The antibacterial activity test against Staphylococcus aureus was performed and revealed the antibacterial activity of nanofibrous membranes loading with Alli. Compared with the nanofibrous membrane without GO, the CS/PVA/Alli nanofibrous membrane with 0.1 wt% GO still had nice antibacterial activity after 48 h. The water contact angle of nanofibrous membranes dropped significantly with the addition of GO and Alli, which showed the nanofibrous membrane had highly hydrophobic. The CS/PVA/GO nanofibrous membrane loading with Alli had great hygroscopicity and moisture-retention capacity. The essential characteristics of nanofibrous membranes were evaluated by SEM, FTIR , XRD. The above results indicate that the membrane has a strong antimicrobial activity and long-lasting efficacy, so the developed natural nanofibrous membranes hold potential as promising antibacterial wound dressing and tissue engineering.
研究领域:
材料科学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
MOLECULAR NUTRITION & FOOD RESEARCH
·
2024
·
IF 4.5
Kelin Li,Victoria A. Uyanga,Xiaojuan Wang,Hongchao Jiao,Jingpeng Zhao,Yunlei Zhou,Haifang Li,Hai Lin
Scope Allicin, a product of enzymatic reaction when garlic is injured, plays an important role in maintaining glucose homeostasis in mammals. However, the effect of allicin on glucose homeostasis in the state of insulin resistance remains to be elucidated. This study investigates the effect of allicin on glucose metabolism using different muscle fibers in a chicken model. Methods and results Day-old male Arbor Acres broilers are randomly divided into three groups and fed a basal diet supplemented with 0, 150, or 300 mg kg −1 allicin for 42 days. Results show that allicin improves the zootechnical performance of broilers at the finishing stage. The glucose loading test (2 g kg −1 body mass) indicates the regulatory role of allicin on glucose homeostasis. In vitro results demonstrate allicin increases glutathione (GSH) level and the expression of cystathionine γ lyase (CSE), leading to endogenous hydrogen sulfide (H 2 S) production in M. pectoralis major (PM) muscle-derived myotubes. Allicin stimulates adenosine monophosphate-activated protein kinase (AMPK) S-sulfhydration and AMPK phosphorylation to promote glucose uptake, which is suppressed in the presence of d , l -propargylglycine (PAG, a CSE inhibitor). Conclusion This study demonstrates that allicin induces AMPK S-sulfhydration and AMPK phosphorylation to promote glucose uptake via the CSE/H 2 S system in a muscle fiber-dependent manner.
研究领域:
动物科学
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营养学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2021
·
IF 9.642
Jingyang Luo,Le Zhang,Wei Du,Xiaoshi Cheng,Fang Fang,Jiashun Cao,Yang Wu,Yinglong Su
T his work revealed the impacts of exogeneous allicins on the antibiotic resistance genes (ARGs) variations during waste activated sludge (WAS) fermentation process. The overall abundance of ARGs was respectively reduced by 4.84 and 9.42% in presence of 0.01 and 0.05 g allicin/g TSS. Allicins disrupted the EPS structure and increased the permeability of cell membranes, which resulted in the release of ARGs for subsequent removal. Allicins also reduced intracellular ATP levels, which was disadvantageous to ARGs dissemination. Besides, allicins affected the microbial community and decreased the abundance of potential hosts based on bacterial taxa-ARGs network analysis. Moreover, the metabolic pathways and genetic expressions ( i.e., two-component system, quorum sensing, and SOS response) involved in ARGs propagation were down-regulated, which caused the ARGs alleviation in allicins-stressed reactors. Overall, the simultaneous responses of cellular status, bacterial host, and genetic regulation accounted for the effective ARGs reduction induced by allicins during WAS fermentation.
研究领域:
环境科学
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微生物学
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DOI
PubMed
高影响因子
JOURNAL OF CLUSTER SCIENCE
·
2022
·Vol. 34
·期 2
·761-771
·
IF 3.447
Fan Zhi-xia,Zhang Jing,Wang Xin,Miao Guo-ying
Squamous carcinoma (SCC) is one of the most common types of cancer in the world. The increasing rates of industrialization and the subsequent environmental changes have aggravated the issue of skin cancer. The stern topical barrier is impaired in diseases like skin cancer while challenging to break. Chemotherapy and surgery are no longer the only ways to treat skin cancers. Hence, we effectively developed allicin-loaded poly (lactic-co-glycolic acid) nanoparticles (NPs), designated as Allicin@NPs, for the treatment of in vitro squamous carcinoma cells has been developed. The Allicin@NPs characterization of the morphology investigation was assessed by the high-resolution transmission electron microscopy. Hydrodynamic parameters and zeta potential were investigated using the dynamic light scattering technique. The in vitro MTT analysis has displayed a substantial inhibition effect of Allicin@NPs on squamous carcinoma cells (B16F10 and A431 cells), and the proliferation of the cells was examined by EdU staining methods. Furthermore, morphological examination of the SCC cells was confirmed by ethidium bromide and acridine orange and Hoechst 33342 nuclear staining methods. The mechanism of apoptosis was further investigated by flow cytometry analysis. Notably, Allicin@NPs have proved that the dramatically improved animal model systemic toxicity profiles of the Allicin is recognized as potential candidates for SCC therapy employing these Allicin@NPs and needs further investigation.
研究领域:
肿瘤学
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药剂学
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纳米医学
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产品关联
DOI
高影响因子
JOURNAL OF FOOD SCIENCE
·
2020
·Vol. 85
·期 10
·3562-3569
·
IF 2.479
Tingting Li,Likun Ren,Dangfeng Wang,Minjie Song,Qiuying Li,Jianrong Li
Low-purine food is not only the focus of gout patients, but also the focus of contemporary green diet development. Fish are usually considered as high-purine foodstuff because of the high nutritional value and high purine content. Therefore, it is necessary to reduce purine content in fish to ensure that they are suitable for patients with hyperuricemia or gout. In this study, the effect of allicin on purine reduction in turbot during cooking was investigated using high-performance liquid chromatography, and the change of xanthine oxidase (XO) activity was also studied. Molecular docking analysis was further performed to elucidate the mechanism of purine reduction by allicin. The results revealed that in the step of soaking, allicin could reduce purine content in fish by slightly enhancing XO activity, promoting hypoxanthine transformation into xanthine. The removal of total purines in experimental and control group reached 70.45% and 57.20%, respectively. Moreover, allicin could change the thermal stability of xanthine by providing an acidic environment, resulting in the rapid decrease of xanthine and hypoxanthine levels by boiling. Thus, this study provides a simple method to decrease purine levels, suggesting a possibility that allicin can function as a purine remover in food.
研究领域:
食品科学
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生物化学
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营养学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Yipeng Pang,Maofang Zhao,Yuhan Xie,Yanping Wang,Yuxin You,Yongding Ke,Chaoyi Zhang,Xiaohan Chen,Yijia Yang,Chunlei Zhang,Xi Chen,Yi Liu,Xingtang Fang
The development of non-antibiotic strategies for combating bacterial infections, particularly through reactive oxygen species (ROS) accumulation accompanying inflammation, has attracted much attention. Despite Allicin (Ac) emerges as a promising antimicrobial agent with wide and remarkable bioactivity, it still displays quite limited ROS scavenging and anti-inflammation capacity due to uncontrolled and poor bioavailability. Herein, a multifunctional nanoplatform with pH-dependent and ROS-scavenging antibacterial, anti-inflammatory and proangiogenic properties is introduced to promote infected wound healing by integrating Ac into silver nanoparticles (AgNPs)-loaded zeolite imidazole framework (ZIF-8), namely Ac@ZIF-8/AgNPs. Primarily, the nanoplatform is activated and controlled release in the infection microenvironment, with disrupting biofilms, scavenging ROS and antibacterial capacitys, outperforming conventional Ac. Moreover, in the mouse models induced by bacterial infection, Ac@ZIF-8/AgNPs can alleviate the inflammatory condition, scavenge ROS, facilitate the regeneration of collagen fiber and enhance vascular formation, collectively accelerating wound healing. Overall, this biocompatible nanoplatform is provided as a reliable approach to solve the long-standing problem of infected wound healing.
研究领域:
纳米技术
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生物医学工程
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药学
·
伤口愈合
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DOI
高影响因子
POLYMER ENGINEERING AND SCIENCE
·
2024
·Vol. 64
·期 10
·4709-4719
·
IF 3.2
Jing Cao,Li Wang,Yuying Zheng
Slow rebound polyurethane foam (SPUF) has been widely used due to its advantages such as sound insulation, energy absorption, and good tactile sensation. However, SPUF is prone to harden at low temperature, and its application in medical equipment and households requires significant antibacterial properties. In this paper, self-made silicone modified polyethylene glycol (Si-APEG) and graphene oxide supported allicin (Alc@GO) were prepared and used as low-temperature resistant agent and antibacterial agent, respectively. Low-temperature resistant polyurethane foam (LSPUF) and antibacterial LSPUF (ALSPUF) were prepared respectively with water as foaming agent. The morphology of ALSPUF was observed by scanning electron microscope. Properties studied include apparent core density and porosity, mechanical properties including tensile strength, 40% compressive hardness, and rebound resilience, as well as low-temperature resistance. Effects of Si-APEG content on the structures and properties were analyzed and the LSPUF with an Si-APEG content of 10 wt.% showed the best comprehensive performance. Therefore, ALSPUFs with Si-APEG content of 10 wt.% and Alc@GO content of 0–3 wt.% were prepared. The addition of Alc@GO increased the antibacterial ratio significantly without obvious effect on the structure and mechanical properties of LSPUF. The antibacterial ratio of ALSPUF reached 99.07% at a Alc@GO content of 2.5 wt.% and testing time of 60 min. This work provides an effective and feasible method for the preparation of ALSPUF which can be widely used in medical devices and households. Highlights Silicone modified polyether is a suitable low-temperature resistant agent for PU foam. Allicin supported on GO provides good antibacterial property for PU foam. ALSPUF has better mechanical properties than SPU.
研究领域:
材料科学
·
生物医学工程
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产品关联
DOI
高影响因子
BIOSCIENCE REPORTS
·
2019
·Vol. 39
·期 6
·
IF 2.535
Qi Feilong,Zhang Chen,Jiang Shanshan,Wang Qian,Kuerban Kudelaidi,Luo Man,Dong Mengxue,Zhou Xinguang,Wu Laiming,Jiang Biao,Ye Li
Allicin can be used as fumigant to protect food and cultural relics from fungal contamination because of its strong antifungal activity and the characteristics of high volatility and no residues. However, the obvious disadvantages such as high minimal inhibitory concentration and instability prevent it from wide application. In this study, a stable derivative of allicin, S-ethyl ethanethiosulfinate (ALE), was synthesized. We further explored its antifungal activity and apoptosis-inducing effect, as well as the underlying mechanism. ALE had an excellent capability of inhibiting spore germination and mycelial growth of Penicillium chrysogenum observed by inverted microscope and scanning electron microscopy. XTT colorimetric assay indicated ALE could reduce the cell viability obviously and IC50 was 0.92 μg/ml, only 1/42 of allicin (38.68 μg/ml). DHR 123 ROS Assay Kit, flow cytometry assay and confocal immunofluorescence revealed intercellular ROS generation and metacaspase-dependent apoptosis triggered by ALE, while antioxidant tocopherol could reverse ALE-induced cytotoxicity effect and metacaspase activation. These results indicate that ALE induces metacaspase-dependent apoptosis through ROS generation, thus possesses an effective antifungal activity. This new derivative of allicin might be developed as a high efficient alternative to the conventional fungicides for food storage and cultural relic protection.
研究领域:
药理学
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微生物学
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食品科学
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文化遗产保护
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2024
·
IF 4
Xiaoyun Zhang,Yinhang Zhang,Yuxin Su,Shuo Guan
Protonated Mannich base N,N′-bis(3-oxo-3-phenylpropyl)ethane-1,2-diamine dihydrochloride (EDAM) was synthesized and its chemical structure was characterized by means of 1 H NMR, 13 C NMR, Fourier-transform infrared spectroscopy (FTIR) and elemental analysis. The synergistic inhibitive effect of EDAM with allicin on N80 steel in 15 % lactic acid solution at 363 K was investigated using weight loss experiments, electrochemical tests, and surface analysis techniques (including scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray Photoelectron Spectroscopy (XPS), and contact angle measurement), quantum chemical calculation and molecular dynamics (MD) simulation methods. The results show that the inhibition performance was significantly improved by combining EDAM and allicin. The inhibition efficacy (IE) reached 99.60 % at the mass ratio of 10:1 (EDAM/allicin) with total inhibitor dosage of 0.1 %. Electrochemical impedance test (EIS) proves that the charge transfer-controlled corrosion process is greatly hindered with the addition of allicin. The small changes in the corrosion potential of Tafel curves indicate that the complex system is a hybrid corrosion inhibition mechanism. SEM, AFM , XPS and contact angle surface analyses suggest that EDAM in cooperation with allicin forms a dense and strongly adsorbed protective film on the metal surface. The density functional theory (DFT) and molecular dynamics simulation (MD) calculations further elucidate the mechanism of the synergistic corrosion inhibition effect.
研究领域:
材料科学
·
腐蚀工程
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化学工程
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表面化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）