Weiliang Shen,Hanhan Xu,Jing Feng,Wei Sun,Guoxing Hu,Yonghong Hu,Wenge Yang
Based on the principle of FRET, we have developed a ratiometric and colorimetric fluorescent probe TFBN, which can specifically recognize SO 2 derivatives (SO 3 2− /HSO 3 − ), and exhibit a transition from red to green fluorescence under 405 nm excitation. The probe TFBN owns the advantages of short response time (<3 min), quantitative detection SO 2 derivatives in two linear ranges, extremely low detection limit (39 nM), large Stokes shift (239 nm) and wide emission window gap (140 nm). In addition, the NBC structure was used as a fluorescent donor for FRET probes for the first time, which expanded the diversity of donors. Importantly, with low toxicity and good biocompatibility, the probe TFBN successfully detects exogenous and endogenous sulfites in living cells. These characteristics endow the probe TFBN can be successfully used in living cells and mouse imaging.
The research of endogenous hydrogen sulfide (H 2 S) biology function, pathological and pharmacological effects has been active in the field of chemists and biologists in recent years. Especially, consequent H 2 S detection mechanisms and biological applications based on fluorescence probe emerged in endlessly, competing ideas, each had its advantage. In facts, the reaction mechanism and luminescence properties play a decisive role in the biology application of H 2 S fluorescence probe. In this despite booming season, in the process of research we still skillfully conceived, in view of H 2 S with small structure being able to do successive nucleophilic substitution in the same molecules, high selective H 2 S subcellular detection was realized. Specifically, we take the cyanine (Cy7) with positive charge as a red fluorophore, through the introduction of disulfide bond and carbonate to build the specific reaction sites of H 2 S, thus avoiding the interference of other sulfur-containing substances in biological systems. After reaction with H 2 S, the Probe released red fluorophore, realizing mitochondria target H 2 S detection. Because the advantages of red fluorescence emission probe, the Probe successfully was applied imaging in mice.
By using an organic framework to fabricate functional biomaterials, novel design and advanced biomedical applications of polymeric microbubbles for in vivo targeting and disease-oriented imaging of tumor vascularization can be developed. The present study describes novel synthetic
protocols to fabricate RGD (Arginine-Glycine-Aspartic)-tagged αvβ3-targeted ultrasound microbubbles. The microbubbles were synthesized by emulsion polymerization techniques. Two types of microbubbles (MBs-1 and MBs-2) were obtained via biotin-streptavidin conjugation
to poly(butyl cyanoacrylate) microbubbles (MBs-0) obtained by one-step synthesis in reverse order. The size distributions and surface zeta potentials were characterized. The results showed that the sizes of the MBs-2 were larger than that those of the MBs-1, and the MBs-2 showed decreased
charge compared to MBs-1. In cell targeting studies, MBs-2 exhibited relatively stronger targeting affinity for αvβ3 integrins, while MBs-1 showed weaker targeting capability. Furthermore, in vivo mice imaging using MBs-2 for intravenous injection exhibited
an obvious and sustained signal increase, which revealed the accumulative of MBs-2 anchoring in tumor. Hence, MBs-2 have been proven to be a promising candidate for using as ultrasound contrast agents for the early diagnosis of αvβ3-overexpressing malignant tumors,
including breast cancer.
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