Frontiers in Bioengineering and Biotechnology
·
2021
·
IF 5.89
Lyu Liting,Chu Yadong,Zhang Sufang,Zhang Yue,Huang Qitian,Wang Shuang,Zhao Zongbao K.
Conversion of lignocellulosic biomass into lipids and related chemicals has attracted much attention in the past two decades, and the oleaginous yeast Rhodosporidium toruloides has been widely used in this area. While R. toruloides species naturally have physiological advantages in terms of substrate utilization, lipid accumulation, and inhibitor resistance, reduced lipid production and cell growth are noticed when biomass hydrolysates are used as feedstocks. To improve the robustness of R. toruloides, here, we devised engineered strains by overexpressing genes responsible for phenolic compound degradation. Specifically, gene expression cassettes of the manganese peroxidase gene (MNP) and versatile peroxidase gene (VP) were constructed and integrated into the genome of R. toruloides NP11. A series of engineered strains were evaluated for lipid production in the presence of typical phenolic inhibitors. The results showed that R. toruloides strains with proper expression of MNP or VP indeed grew faster in the presence of vanillin and 5-hydroxymethylfurfural than the parental strain. When cultivated in concentrated mode biomass hydrolysates, the strain VP18 had improved performance as the cell mass and lipid content increased by 30% and 25%, respectively. This study provides more robust oleaginous yeast strains for microbial lipid production from lignocellulosic biomass, and similar efforts may be used to devise more advanced lipid producers.
研究领域:
生物技术
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微生物学
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产品关联
DOI
PubMed
高影响因子
Microorganisms
·
2025
·Vol. 13
·期 3
·612
·
IF 4.2
Wenming Wang,Liling Xie,Lin Zhao,Qilin Yu
Alum mining leads to significant heavy metal and acid pollution within soils. Phytoremediation is a common strategy used to treat alum mine soils, but its efficiency is frequently compromised by the alum-mining-induced impairment of plant growth. To improve the strength of plants against mine pollution, this study constructed the artificial yeast strain ScHB (heavy metal-binding protein-containingSaccharomyces cerevisiae) expressing the de novo designed protein HBGFP (heavy metal-binding green fluorescence protein) and investigated its effect on the phytoremediation of alum mine soils with soil physiochemical assays and heavy metal quantification. This protein was composed of an N-terminal signal peptide, an HB (heavy metal-binding) domain, and a GFP (green fluorescence protein) domain, as well as a C-terminal glycolphosphatidylinositol-anchoring fragment. The exposure of the HBGFP on the ScHB surface increased the growth rate of the yeast cells and enhanced cadmium capture from the cadmium-containing medium. After culturingMedicago sativain the alum mine soils for 30 days, ScHB remarkably increased the plants’ average height from 17.5 cm to 27.9 cm and their biomass from 3.03 g/plant to 4.35 g/plant, as well as increasing the accumulation of antioxidant agents in the plants. Moreover, the ScHB cells strongly improved the soil quality, with an increase in the soil pH values from 5.47 to 6.21 to 6.9, and increased the levels of soil organic matter, total nitrogen, available phosphorus, and living bacteria. Furthermore, ScHB efficiently improved the plants’ abilities to remove soil heavy metals, decreasing the levels of cadmium, lead, chromium, and copper by 90%, 86%, 97%, and 88%, respectively. This study developed a genetic engineering method to improve the efficiency of phytoremediation against pollution from alum mining.
研究领域:
环境科学
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遗传工程
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植物学
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产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2015
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IF 4.494
Hong Guo,Shaolan Zou,Boshi Liu,Rongxin Su,Renliang Huang,Wei Qi,Minhua Zhang,Zhimin He
Enzyme recycling by re-adsorption is one of the primary methods for reducing enzyme usage in lignocellulose conversion. This work proposes the combination of an engineered yeast strain that expresses β-glucosidase with enzyme recycling to reduce the amount of supplemented β-glucosidase in enzyme recycling experiments. Using the engineered strain, a slight increase in ethanol concentration was obtained after a 96-h fermentation of pretreated corncobs. Ethanol concentrations increased by 34.7% and 62.7% in the following two recycle rounds using the engineered strain compared with those using its parental strain without β-glucosidase addition. Furthermore, with the addition of β-glucosidase at 30 CBU/g cellulose, the ethanol concentration after two recycle rounds exceeded 90% of that observed in the first SSF round with the engineered strain at a high initial cellulase loading of 45 FPU/g cellulose.
研究领域:
生物技术
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酶工程
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发酵工程
✓ 已验证
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
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IF 8.2
Qing-Song Huang,Si-Qi Chen,Xiao-Min Zhao,Li-Jun Song,Yu-Ming Deng,Ke-Wei Xu,Zheng-Fei Yan,Jing Wu
Polyethylene terephthalate (PET) microplastics pose significant environmental and human health risks due to their resistance to degradation and accumulation in ecosystems. In this study, we engineered Stenotrophomonas pavanii JWG-G1, a robust biofilm-forming bacterium, to overexpress the PET hydrolase (DuraPETase) for PET microplastics degradation at ambient temperature. Nine endogenous PET hydrolases were identified through genome sequencing of S. pavanii , and were successfully expressed in Escherichia coli BL21(DE3). Among them, hydrolase Est_B achieved 100% degradation of bis(2-hydroxyethyl) terephthalate (BHET) at an initial concentration of 0.23 mg/mL at 30 °C within 4 h, identifying it as a novel BHETase. However, the PET degradation performance of all endogenous PET hydrolases was inferior to that of DuraPETase. The engineered strain overexpressing DuraPETase demonstrated a significant enhancement in PET degradation, achieving a 38.04 μM total product release of high-crystallinity PET microplastics after 30 days at 30 °C. The degradation extent was greater than that of low biofilm-forming engineered strains, attributing to the aggregation of DuraPETase on the PET surface in the presence of biofilm. Additionally, this engineered strain also maintained PET degradation activity across various water environments and demonstrated effectiveness in degrading other polyester plastics. This is the first report demonstrating that an engineered strain of Stenotrophomonas species is capable of simultaneously secreting exogenous hydrolase and degrading polyester microplastics, representing a novel approach in the development of engineered bacteria with potential applications in bioreactor systems and environmental remediation.
研究领域:
环境科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
AMB Express
·
2021
·Vol. 11
·期 1
·1-8
·
IF 3.298
Xu Jing,Wang Bo,Zhang Wen-hui,Zhang Fu-Jian,Deng Yong-dong,Wang Yu,Gao Jian-Jie,Tian Yong-Sheng,Peng Ri-He,Yao Quan-Hong
p-Nitrophenol (PNP) is an important environmental pollutant and can causes significant environmental and health risks. Compared with the traditional methods, biodegradation is a useful one to completely remove the harmful pollutants from the environment. Here, an engineered strain was first constructed by introducing PNP biodegradation pathway via the hydroquinone (HQ) pathway into Escherichia coli. In the engineered strain BL-PNP, PNP was completely degraded to β-ketoadipate and subsequently enter the metabolites of multiple anabolic pathways. The high tolerance and rapid degradation ability to PNP enable the engineered strain to have the potential to degrade toxic substances. The engineered strain created in this study can be used as a functional strain for bioremediation of PNP and potential toxic intermediates, and the method of assembling aromatic hydrocarbons metabolic pathway can be used to eradicate nitroaromatic pollutants in the environment.
研究领域:
环境科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
BIOTECHNOLOGY AND BIOENGINEERING
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2016
·Vol. 114
·期 3
·543-551
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IF 4.243
Renliang Huang,Hong Guo,Rongxin Su,Wei Qi,Zhimin He
Recycling cellulases by substrate adsorption is a promising strategy for reducing the enzyme cost of cellulosic ethanol production. However, β-glucosidase has no carbohydrate-binding module (CBM). Thus, additional enzymes are required in each cycle to achieve a high ethanol yield. In this study, we report a new method of recycling cellulases without β-glucosidase supplementation using lignocellulosic substrate, an engineered strain expressing β-glucosidase and Tween 80. The cellulases and Tween 80 were added to an aqueous suspension of diluted sulfuric acid/ammonia-treated corncobs in a simultaneous saccharification and fermentation (SSF) process for ethanol production. Subsequently, the addition of fresh pretreated corncobs to the fermentation liquor and remaining solid residue provided substrates with absorbed cellulases for the next SSF cycle. This method provided excellent ethanol production in three successive SSF cycles without requiring the addition of new cellulases. For a 10% (w/v) solid loading, a cellulase dosage of 30 filter paper units (FPU)/g cellulose, 0.5% Tween 80, and 2 g/L of the engineered strain, approximately 90% of the initial ethanol concentration from the first SSF process was obtained in the next two SSF processes, with a total ethanol production of 306.27 g/kg corncobs and an enzyme productivity of 0.044 g/FPU. Tween 80 played an important role in enhancing cellulase recovery. This new enzyme recycling method is more efficient and practical than other reported methods. Biotechnol. Bioeng. 2017;114: 543–551. © 2016 Wiley Periodicals, Inc.
研究领域:
生物技术
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酶工程
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发酵工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Jiazeng Yang,Zhuoxin Guo,Wenlong Zha,Jiachen Zi
Piceatannol, a stilbene widely found in many types of foods, shows a variety of effects on the cardiovascular system, nervous system, and skin. Its low abundance in nature impedes its development. Microbial cell factories offer a green and sustainable approach to the production of natural products. In this study, we screened two 4-coumarate:coenzyme A ligases (4CLs), nine stilbene synthases (STSs), and five 4-hydroxyphenylacetate-3-hydroxylases (4HPA3Hs) to construct multiple bioconversion routes from p-coumaric acid to piceatannol in a single Saccharomyces cerevisiae strain. Then, the supply of p-coumaric acid and malonyl-CoA was enhanced, and NADPH and FADH2 regenerations were optimized, resulting in establishment of yPi101 which produced 127 mg/L and 442 mg/L piceatannol in a flask and by fed-batch fermentation, respectively. This is the first report of the de novo production of piceatannol in a single S. cerevisiae strain and represents the highest piceatannol yield.
研究领域:
合成生物学
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微生物工程
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天然产物合成
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产品关联
DOI
PubMed
高影响因子
ACS Synthetic Biology
·
2021
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IF 5.11
Guangbao Zhang,Xiaohui Yang,Zhenhua Zhao,Tao Xu,Xiaoqiang Jia
Polycyclic aromatic hydrocarbons (PAHs) are highly toxic and persistent organic pollutions that can accumulate in the environment. In this study, an aromatic ring cleavage module, a salicylic acid synthesis module, and a catechol metabolism module were respectively constructed in three Escherichia coli BL21 strains. Subsequently, the engineered strains were cocultured as an artificial consortium for the biodegradation of phenanthrene, a typical PHA. Single factor experiments and response surface methodology were used to identify the optimal degradation conditions, including an inoculation interval of 6 h, inoculation ratio of 1:1:1, and IPTG concentration of 2 mM. Under these conditions, the 7-day degradation ratio of 100 mg/L phenanthrene reached 72.67%. Moreover, the engineered Escherichia coli BL21 strains showed good phenanthrene degradation ability at substrate concentrations 10 mg/L up to 500 mg/L. Enzyme activity assays combined with gas chromatography–mass spectrometry measurements confirmed that the three engineered strains behaved as a synergistic consortium in the phenanthrene degradation process. Based on the analysis of the key metabolites, the engineered bacteria were supplemented at 7-day intervals in batches so that each engineered strain maintained its optimal degradation ability. The 21-day degradation ratio finally reached 90.66%, which was much higher than what was observed with simultaneous inoculation. These findings suggest that the three engineered strains with separate modules constructed in this study offer an attractive solution for removing PAHs from the environment.
研究领域:
环境生物学
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微生物学
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产品关联
DOI
PubMed
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
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2024
·
IF 3.5
Guo Xudong,Wang Dingyi,Guo Yingying,Zhang Junpeng,Li Yingying,Tian Haozhong,Liu Lihong,Liang Yong,Yin Yongguang,He Bin,Hu Ligang,Jiang Guibin
Droplet-based bioprinting (DBB) allows for high precision, noncontact, and on-demand distribution of bioinks, hence it has been widely utilized in the preparation of bacteria-laden living materials (BLMs). Nonetheless, discontinuous ink deposition makes it challenging to fabricate large-sized intact living structures via this technique. Herein, we explore the way of using DBB to construct centimeter-scale BLMs with bespoke geometries, and further demonstrate its potential applicability in sensing-responsive device by integrating engineered bacteria. We first established a DBB method based on printing-path design, which does not require hardware modification. This strategy was able to produce customized 3D-hydrogel structures with high shape fidelity. Then, we confirmed the excellent biocompatibility of the above biofabrication approach. The Escherichia coli survived 93% ± 4.0% in printed BLMs, with uniform distribution throughout the structure. As a proof-of-concept, we finally manufactured a test strip-like heavy metal biosensor capable of plug-and-play detecting mercury (II) in water using the aforesaid approach. To our knowledge, this is the first study to employ 3D bioprinted BLMs for the detection of prevalent heavy metal pollutants. Our research shed light on the versatility of DBB in BLMs construction, which is not restricted to two-dimensional patterns. Moreover, our results are expected to innovate heavy metal biodetection and improve detection efficiency and sensitivity. Graphical abstract
研究领域:
生物制造
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合成生物学
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环境科学
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Wenwei Tang,Meihuan Tong,Hui Liao,Peiqi Zhang,Xinping Zeng
Aureomycin (CTC) is a commonly used tetracycline antibiotic that causes water and soil pollution and greatly endangers the environment and human health. Microbial degradation to remove residual antibiotics in wastewater is of growing interest. Due to its unique structure and excellent oxidative properties, biogenic manganese oxides (BMO) has been widely studied in the field of water pollutant removal. However, the current preparation of BMO generally suffers from low yield and insufficient production efficiency. In this paper, an efficient manganese-oxidizing bacterium Bacillus sp. D-1 was isolated. Manganese-oxidizing engineering bacteria were constructed to achieve rapid and sufficient BMO production, and the structure and function of the BMO was characterized. SEM was used to characterize the layered structure of the nanoparticles. XPS and XRD were used to determine that the mixture was composed of manganese species with three valence states. BMO was used to degrade 10 mg·L −1 CTC, and the CTC removal rate reached 93.2 % in 24 h, with an optimal reaction pH of 7.6. Finally, LCMS and ECOSAR were used to analyze the oxidative degradation products of CTC and their biological toxicity. Therefore, this paper successfully constructed a high-performance manganese-oxidizing engineering bacterium, revealed the efficient degradation mechanism of CTC by BMO, provided a new strategy for the green treatment of combined pollution of manganese and antibiotics in water bodies, and realized the continuous removal of manganese and water pollutants.
研究领域:
环境微生物学
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环境工程
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分子生物学
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污染物生物修复
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水处理技术
✓ 已验证
产品关联
DOI
高影响因子
Advanced Healthcare Materials
·
2025
·
IF 9.6
Qiliner Feng,Chen Wang,Si Shi,Junyu Fan,Yurong Chen,Tuanjie Zhang,Zhijun Ruan,Yongzheng Ma,Zifu Li,Jie Liu,Baizhu Chen
Tumor cells reprogram the energy metabolism and re-shape the microenvironment to maintain their fast proliferation and metastasis, leading to immunosuppression. Inspired by the design of engineered living materials, in this study, the AND-gated living hydrogel for metabolic-regulation enhanced tumor immunotherapy is constructed. Bacteria are genetically rewired to express lactate oxidase or glucose oxidase as two inputs under the control of thermosensitive promoter and then encapsulated inside the NIR-light controlled hydrogel. Triggered by laser, the engineered living hydrogel weakened the glycolysis and improved the mitochondrial respiration of tumor cells. The regulation of energy metabolism potentiated the antitumor immune responses by stimulating T cells, polarizing tumor associated macrophages to M1 phenotype, inducing the immunogenic cell death and stimulating the cGAS/STING pathway. With “high” inputs of two enzymes, the engineered living hydrogel realized the enhanced tumor immunotherapy as the “high” output. An approach of living hydrogel for metabolic-regulation AND-gated tumor immunotherapy is established.
研究领域:
肿瘤学
·
免疫学
·
合成生物学
·
生物材料
·
分子医学
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转化医学
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纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2024
·Vol. 14
·期 1
·2402272
·
IF 10
Hongwei Zhang,Linfu Chen,Qian Chen,Qiufang Chen,Jun Zhou
Tirapazamine (TPZ), an antitumor prodrug, can be activated in hypoxic environment. It specifically targets the hypoxic microenvironment of tumors and produces toxic free radicals. However, due to the tumor is not completely hypoxic, TPZ often fails to effectively treat the entire tumor tissue, resulting in suboptimal therapeutic outcomes. Herein, a low pathogenic Escherichia coli TOP10 is utilized to selectively colonize tumor tissues, disrupt blood vessels, and induce thrombus formation, leading to the expansion of hypoxic region and improving the therapeutic effect of TPZ. Additionally, a thermosensitive hydrogel is constructed by Pluronic F-127 (F127), which undergoes gelation in situ at the tumor site, resulting in sustained release of TPZ. To monitor the therapeutic process, it is genetically modified TOP10 by integrating the bioluminescent system luxCDABE (TOP10-Lux). The bioluminescent signal is associated with tumor hypoxia enhancement and thrombus formation, which is beneficial for therapeutic monitoring with bioluminescence imaging. In the murine colon cancer model, the TOP10-Lux combined with TPZ-loaded F127 hydrogel effectively suppressed tumor growth, and the treatment process is efficiently monitored. Together, this work employs genetically modified TOP10-Lux to enhance the therapeutic efficacy of TPZ and monitor the treatment process, providing an effective strategy for bacteria-based tumor-targeted chemotherapy and treatment monitoring.
研究领域:
肿瘤学
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微生物治疗
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分子影像学
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药物递送
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生物材料
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转化医学
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合成生物学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）