Journal of Materials Chemistry C
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2021
·Vol. 9
·期 12
·4300-4306
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IF 7.393
Yanmin Zhao,Xin Geng,Xinxin Shi,Yifei Guo,Yuanqiang Sun,Lingbo Qu,Zhaohui Li
Developing optical oxygen-sensitive nanoparticles with good biocompatibility and low cost to rapidly detect molecular oxygen in a signal turn-on mode is highly desirable. Herein, we present a phenylenediamine-derived carbon dot (CD), which has been found to display a fluorescence-switchable property, with fluorescence quenching by Na2S2O4 and fluorescence recovery triggered by O2. Because of this unique property, we directly employed the quenched CDs as a reversible probe for the fluorescence turn-on detection of molecular oxygen and found that this detection mode possessed a short response time of 12 s and high sensitivity. Moreover, this CD solution could be used as a writeable ink for the patterning of paper strips and security service without any loss in the fluorescence-switchable property. We thus achieved CD-based test strips for oxygen detection in vacuum packaging. This study not only provides a convenient, low-cost and practical way for oxygen detection but also reveals a fluorescence-switchable CD that will find its applications in numerous O2-involved fields.
研究领域:
化学
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生物传感
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材料科学
✓ 已验证
DOI
高影响因子
MATERIALS LETTERS
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2017
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IF 2.572
Shenghong Yang,Xiaohan Sun,Yu Chen
The rapid development of fluorescent nanomaterials has broadened a new horizon for the design of easy-to-use and sensitive sensors. Herein, the stable, water-soluble and fluorescent L-Cystine modified copper nanoclusters (CuNCs) were prepared via size-focusing etching method. The maximum emission wavelength of the obtained CuNCs was 500 nm with an excitation wavelength of 396 nm. The as-prepared CuNCs can only emit faint light when dispersed in aqueous solution, but generate obvious fluorescence intensity enhancement after adding specific amount of 2,4,6-trinitrotoluene (TNT). Under the optimal conditions, the CuNCs were adopted as an effective fluorescence probe for the detection of trace TNT through fluorescence “turn-on” mode for the first time. The calibration curve of TNT was linear up to 4.8 μM, with a detection limit of 9.1 nM.
研究领域:
分析化学
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环境科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Xiaoxia Li,Yuchen Luo,Shufang Zhou,Jiayi Wang,Futai Lu,Shuo Wang,Qiliang Deng
A sensor capable of sensing of water in various organic solvents ranging from water-soluble to water-miscible solvents is still a challenging task. In this research, a cyclic polymer fluorescence chemosensor (CPFC) has been developed for sensing of water by turn-on model in 9 organic solvents and turn-off model in DMA, where the broadest concentration range and the lowest detection limit was obtained for water in DMA (10 %–90 %) and dioxane (0.011 %), respectively. The sensing mechanism is explored by theory calculation and experimental investigation. The amphiphilic nature endows the polymer probe with great potential for measuring various contaminants from aqueous and nonaqueous mediums. Furthermore, the present search highlights the potential applications of cyclic polymer as fluorescence probes in the field of sensing.
研究领域:
材料科学
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分析化学
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聚合物科学
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环境科学
✓ 已验证
DOI
PubMed
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 9
·1248-1253
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IF 2.378
Dehua Deng,Yuanqiang Hao,Ping Yang,Ning Xia,Wenxue Yu,Xiuhua Liu,Lin Liu
A general peptide substrate for the specific detection of proteases is linked with a quencher/fluorophore pair. However, labeling of the substrate with a quencher/fluorophore pair may increase the complexity for the synthesis of peptides and affect the approach of protease to the cleavage site. A peptide with a specific sequence has been shown to bind Cu2+ with high affinity. More intriguingly, Cu2+ exhibits a high inherent quenching ability towards the fluorophore by complexation with the sequence-specific peptide. Based on this fact, this work reports a general single-labeled peptide substrate for the detection of protease activity, screening of potential inhibitors and evaluation of cell apoptosis. The feasibility and applications of the method were demonstrated by assays of caspase-3 and β-secretase with a signal-on and a signal-off format, respectively.
研究领域:
生物化学
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分子生物学
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分析化学
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药物发现
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2014
·Vol. 181
·期 11
·1361-1367
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IF 3.719
Luo Xubiao,Wu Wenbin,Deng Fang,Chen Dezhi,Luo Shenglian,Au Chaktong
Silica-coated, thiol-enriched and zinc-doped CdS quantum dots (SiO 2 -S-Zn-CdS QDs) were fabricated by a sol–gel process and used as fluorescent probe for imaging and monitoring of intracellular Cd(II) and Zn(II) in endophytes in the “turn-on” mode. There is a linear relationship between fluorescence intensity and the concentration of Zn(II) in the 3.5 – 23.2 μM concentration range and with a detection limit of 2.0 μM. The probe also responds well to Cd(II) in the 0.3 – 26 μM concentration range and the detection limit is 0.1 μM. Due to its negligible cytotoxicity, fair sensitivity and good photostability, the quantum dot probe presented here is a promising tool for fluorescent imaging of intracellular Zn(II) or Cd(II).
The SiO 2 -S-Zn-CdS QDs “turn-on” fluorescent probe shows superior selectivity for Zn 2+ and Cd 2+
研究领域:
材料科学
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纳米技术
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分析化学
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生物成像
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2020
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IF 10.257
Qiong Hu,Yu Bao,Shiyu Gan,Yuwei Zhang,Dongxue Han,Li Niu
As one of the most important proteolytic enzymes , trypsin is useful as a reliable and specific biomarker for the diagnosis of pancreatitis and other pathological conditions. In this paper, a novel signal-on electrochemical biosensor based on the use of electrochemically controlled grafting of polymers as an amplification strategy is described for the ultrasensitive assay of trypsin activity. The carboxyl-group-free peptide, serving as the substrate for the recognition of trypsin, is first immobilized via its N-terminus. The tryptic cleavage of peptide substrate can generate a free carboxyl group at the C-terminus of the truncated peptide, to which through the carboxylate-Zr(IV)-carboxylate linkage the carboxyl-group-containing initiator for atom transfer radical polymerization (ATRP) can be conjugated. The subsequent surface-initiated grafting of polymers (SI-GOP) based on electrochemically controlled ATRP (eATRP), with ferrocenylmethyl methacrylate (FcMMA) as the monomer , can bring a large amount of Fc tags to electrode surface , resulting in the generation of a very high detection signal. The eATRP-based SI-GOP is easy to operate and low-cost as an amplification strategy. Under optimal conditions, the detection limit for trypsin activity can be down to 0.016 mU mL −1 (~2.68 pM or ~0.064 ng mL −1 ). As the current signal increases with trypsin activity, this trypsin biosensor is less susceptible to false positives due to the signal-on mode. Moreover, it is highly selective and applicable to inhibitor screening and the assay of trypsin activity in the presence of complex biological matrices. Taking together, this electrochemical trypsin biosensor may hold great potential in diagnostic applications.
研究领域:
分析化学
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生物化学
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生物传感器
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诊断学
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电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）