ACS Applied Bio Materials
·
2021
·
IF 0
Pengzhong Shi,Daozhen Zhou,Yaxin Zhu,Bo Peng,Nannan Shao,Xingjie Zan
Fast hemostasis, biosecurity, and adaptation of different bleeding sites are the major challenges for hemostatic materials. In this work, an easy-to-make, rapid hemostatic, tannic acid doped calcium carbonate microsphere with a low concentration of thrombin (Thr@TA-CaCO3) was developed. In an in vitro blood clotting assay, the hemostatic effect of Thr@TA-CaCO3 varied with the change of thrombin amount. Furthermore, the average hemostatic time can be reduced from 205 to 20 s as the thrombin levels are elevated. When applied in different hemorrhage models, the Thr@TA-CaCO3-treated group had a lower blood loss than the tannic acid modified CaCO3 (TA-CaCO3) groups. Especially in the rabbit artery injury model, the total bleeding amount in the Thr@TA-CaCO3-treated group was only 11.97% of that in the TA-CaCO3 groups, proving that Thr@TA-CaCO3 had an excellent hemostatic effect even in the case of rapidly flowing blood. Based on the above, Thr@TA-CaCO3 is a promising hemostatic agent and can be applied in more extensive clinical applications.
研究领域:
生物医学工程
·
材料科学
·
血液学
✓ 已验证
DOI
Advanced Healthcare Materials
·
2025
·
IF 9.6
Weihang Wu,Hongjian Huang,Zhicheng Huang,Qinghui Wang,Zongxuan Huang,Hongwen Zhang,Dongsheng Chen,Wen Wang,Hu Zhao,Haiqing Liu,Baodong Zheng,Yu Wang
Uncontrollable hemorrhage from deep, narrow wounds poses a critical threat to life, as locating the bleeding arteries in such areas is challenging. Rapid bleeding control remains a significant challenge in pre-hospital care. Herein, an injectable hemostatic device filled with disc-like PVA sponges is developed for this objective. The pristine PVA sponge is strung as a necklace-structured sponge loaded in a foldable injector. With a novel retraction mechanism, this self-designed injector precisely deploys hemostatic sponges to deep bleeding sites, surpassing the XStat TM injector in design innovation. The PVA sponges' high porosity and unique porous structure allowed it for rapid shape recovery (<30 s) and good compressive strength. In the punctured wound, the swollen PVA sponges exert high compression to close the bleeding artery, resulting in fast-bleeding control. In the pig groin femoral artery injury model, the PVA sponge treatment resulted in a 100% survival rate during a 2-h hemostasis period, compared to a 33.3% survival rate in the cotton gauze control group. In addition, it can be easily taken out by a simple pulling. The easily applicable and removable necklace-structured PVA sponge is a highly promising hemostat for punctured wounds.
研究领域:
生物医学工程
·
创伤急救
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Small
·
2025
·
IF 12.1
Yumei Wang,Han Wu,Xin Liu,Zekai Ren,Yang Cao,Hailin Cong,Bing Yu
The development of biomaterials with rapid hemostatic and antibacterial activity is essential for the treatment of chronic bacterial infected wounds. In this study, bacterial membrane chromatography combined with one-bead-one-compound (OBOC) method is used for rapid screening of antimicrobial peptides with potential antimicrobial properties. The screened antimicrobial peptide LKAPI (AMP LI5) has broad-spectrum bactericidal properties. Besides, AMP LI5 exhibited superior activation of the coagulation cascade and increased adherence to blood cells and platelets, which allows it to quickly coagulate blood. The hemostatic results showed that the AMP LI5 has a desirable hemostatic effect on the mouse tail-breaking model (9 ± 0.47 s). Finally, an HA-base composite antimicrobial peptide hydrogel is prepared antibacterial and hemostatic effects, which have injectability, compressibility, self-healing, and good biocompatibility. In addition, the antimicrobial peptide hydrogel significantly accelerated wound healing in chronic bacterial infected wounds in mouse. In summary, antimicrobial-hemostatic peptide hydrogels are expected to be competitive and multifunctional wound dressings for hemostasis and healing of bacterial-infected wounds.
研究领域:
生物材料学
·
医学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2017
·
IF 4.811
Tingsheng Yan,Feng Cheng,Xinjing Wei,Yudong Huang,Jinmei He
Efficient and biodegradable hemostatic materials become increasingly important in civilian and military clinical. However, traditional hemostatic materials are difficult to achieve expected effects especially in parenchymal organs with rich vascularity. In facing these challenges, we designed a biodegradable collagen sponge reinforced with chitosan/calcium pyrophosphate nanoflowers (CPNFs-Col sponge) for rapid hemostasis. With specific performances, such as rapid water absorption ability, the positive surface rich in amino groups and high specific surface area (952.5 m 2 g −1 ), the obtained CNPFs-Col sponge with optimized composition could activate the intrinsic pathway of coagulation cascade, induce haemocytes and platelets adherence, promote the blood clotting and achieve hemorrhage control in vitro and in vivo. In addition, the CNPFs-Col sponge can be completely biodegraded in 3 weeks, which is suitable for post-operative treatment and peritoneal adhesion prevention. It can be concluded that the CPNFs-Col sponge would become a promising candidate for clinical hemostatic applications.
研究领域:
生物医学材料
·
血液学
·
创伤治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2022
·Vol. 11
·期 4
·852-864
·
IF 7.571
Zhenhua Huang,Juan Wu,Yujiao Zhao,Dong Zhang,Laiqiang Tong,Fan Gao,Changsheng Liu,Fangping Chen
Death caused by excessive blood loss has always been a global concern. Timely control of bleeding in incompressible penetrated wounds remains a great challenge. Here, we developed a shape memory sponge (SQG) based on modified starch and gelatin (Gel) to control the hemorrhage of penetrating wounds. The porous structure of SQG greatly enhanced the absorption of blood, and the adhesion of erythrocytes and platelets. The water absorption rate of SQG reached 1178.72 ± 12.18% in 10 s. SQG quickly recovered its shape in water (∼3 s) and exhibited high mechanical strength (∼38 kPa), acting as a physically packed barrier to facilitate hemostasis. Furthermore, the positively charged sponges were conducive to activating platelets and promoting the release of coagulation factors. SQG sponges possessed the lowest blood coagulation index (BCI) of 21.32 ± 0.19%, and presented good biocompatibility and obvious inhibitory effect on Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). Moreover, SQG sponges controlled complete bleeding in 69 ± 20 s and a bleeding loss of 334 ± 138 mg was observed, nearly 50% lower than that of gelatin sponge in rabbit liver penetrating wounds. Overall, SQG possesses a combination of potent shape recovery, rapid hemostasis, and excellent antibacterial and degradation ability, enabling promising applications for hemostasis in non-compressible penetrating wounds.
研究领域:
生物医学工程
·
材料科学
·
创伤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Aihong Chen,Lixue Wu,Kaiyang Wang,Long Qin,Kexin Zhang,Song Chen,Yijiong Zhang,Wei Song,Qian Zhang,Wei Xia,Yu Luo,Xijian Liu,Jian Wan
Uncontrollable bleeding and excessive blood loss are the primary causes of death on the battlefield and in accidents. However, commercial hemostatic materials possess individual drawbacks for hemostasis. Here, we present a rapid hemostatic powder (SACC-GO) with high biosafety, which is fabricated by a facile ball milling method using inexpensive raw materials (sodium alginate (SA), calcium chloride, cerium nitrate and graphene oxide (GO)). The SACC-GO could immediately become gel when meeting blood, whose strength was enhanced due to specific surface area of GO and high coordination capacity of Ce 3+ ions. In addition, the SACC-GO had a high water absorption rate (~2000 %) allow it to concentrate blood and release Ca 2+ ions to improve blood coagulation. The in vitro and in vivo results, such as hemostasis in tail amputation, femoral hemostasis and hepatic hemostasis, exhibited excellent hemostasis effects by reducing hemostasis time and blood loss (with the majority of reductions exceeding 50 %). The SACC-GO also enhanced wound healing due to Ce ions scavenging reactive oxygen species (ROS). Thus, the SACC-GO hemostatic powder had great potential for rapid hemostasis.
研究领域:
生物医学材料
·
创伤急救
·
止血剂开发
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Youjing Shi,Lijie Wang,Wei Wei,Sheng Ding,Wen Zhang,Fan Li,Zhaoming Li,Xin Meng
Rapid and effective hemostasis can immediately control blood loss and prevent fatal complications. Chitosan sponge loaded with carbon nanospheres PC-800 for rapid hemostasis was first reported here. Preparation process is simple, green and low-cost. Specifically, microporous carbon nanospheres (PC-800) were synthesized by hydrothermal method and followed by calcination in high yield. Afterwards, PC-800 was homogeneously dispersed in the chitosan solution and lyophilized to obtain the composite sponge. The aim of this study is to explore the synergistic hemostatic effect of chitosan and PC-800 and validate its feasibility in trauma therapy. The composite sponges exhibited porous structure, suitable mechanical properties, strong water absorption capacity (49.2 ± 0.8 g/g), good hemocompatibility (hemolysis rate<5 %) and low cytotoxicity. However, cytotoxicity occurs with excessive PC-800 loads. The blood coagulation index of CS-1.0 with 9.5 % was significantly lower than the commercially available Celox Rapid. Upon application to a wound, CS-1.0 sponge rapidly forms a wound seal, achieving effective hemostasis through the synergistic action of chitosan and PC-800. Compared to Celox Rapid, tail hemorrhage and hepatic hemorrhage time in rats decreased to 73 s and 34 s, respectively. The composite sponges were expected to be a safe and fast hemostatic dressing for uncontrolled bleeding during emergencies.
研究领域:
生物医学工程
·
材料科学
·
止血材料研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMERS FOR ADVANCED TECHNOLOGIES
·
2023
·Vol. 34
·期 5
·1608-1621
·
IF 3.4
Xujian Li,Xumin Chen,Zhixiao Ji,Luqi Pan,Yi Liu,Xiao Yang,Changcan Shi
Rapid hemostasis, biosafety, and adaptability to different bleeding sites are the focus of research on hemostatic materials. In this work, an efficient and simple preparation of aldehyde starch hemostatic microspheres (ASHMs) crosslinked with L-cystine dimethyl ester was developed. Through a series of physicochemical parameters and in vitro and in vivo hemostatic properties measurements, the results showed that under the optimal crosslinking degree, ASHMs had a regular and uniform spherical shape, and it had a liquid suction capacity of up to 880%. At the same time, the aldehyde groups on its surface can increase the adhesion and aggregation of blood cells, thereby enhancing the hemostatic effect, which can be considered as a synergistic effect. More importantly, ASHMs have good biocompatibility and minimal intradermal irritation. Taken together，ASHMs could be used as potential novel hemostatic materials for surgical treatment.
研究领域:
生物材料学
·
医学工程
·
外科治疗
✓ 已验证
产品关联
DOI
高影响因子
Nature Communications
·
2022
·Vol. 13
·期 1
·1-14
·
IF 17.694
He Huaying,Zhou Weikang,Gao Jing,Wang Fan,Wang Shaobing,Fang Yan,Gao Yang,Chen Wei,Zhang Wen,Weng Yunxiang,Wang Zhengchao,Liu Haiqing
Cotton gauze is a widely used topical hemostatic material for bleeding control, but its high blood absorption capacity tends to cause extra blood loss. Therefore, development of rapid hemostatic cotton gauze with less blood loss is of great significance. Here, we develop an efficient hemostatic cotton gauze whose surface is slightly modified with a catechol compound which features a flexible long hydrophobic alkyl chain terminated with a catechol group. Its hemostatic performance in animal injuries is superior to standard cotton gauze and Combat Gauze TM . Its biosafety is similar to cotton gauze and rebleeding hardly occurs when the gauze is removed. Here, we show its hemostatic capability is attributable to the rapid formation of big and thick primary erythrocyte clots, due to its effective controlling of blood movement through blocking effect from tissue adhesion by catechol, blood wicking in cotton, and the hydrophobic effect from long alkyl chains.
研究领域:
生物医学工程
·
材料科学
·
血液学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Weishi Zheng,Zheng Zhang,Yuyu Li,Lujie Wang,Feiya Fu,Hongyan Diao,Xiangdong Liu
Traditional hemostatic materials on the market have considerable limitations for coping with massive bleeding. Herein, we developed an injectable hemostatic sponge via a pullulan oxidation approach. After an oxidation using NaIO 4 at room temperature, the resulting oxidized pullulan provides aldehyde groups to cross-link with the residual hydroxyl groups via the acetal reaction, leading to a hydrogel for lyophilization, thereby producing a porous sponge having highly interconnected macro-pores 89.4–195.4 μm in size. Experimental results show that the oxidized pullulan (OP) sponge possesses good biocompatibility and hemocompatibility. The optimized sponge, [email protected] (prepared using 0.5:1 mass ratio of NaIO 4 and pullulan), exhibited a high fluid absorption ratio, excellent shape memory property, and robust mechanical elasticity. In addition, the [email protected] sponge realized a remarkable hemostatic property, which can be reflected by a lower whole blood clotting index and better aggregation capacities of both blood cell and platelet when compared to commercial gelatin sponge and medical gauze. Furthermore, in vivo hemostatic results demonstrate that [email protected] sponge can accomplish rapid hemostasis times for a rat femoral artery injury model (mean value of 40 s) and liver volume defect injury model (mean value of 33.8 s). In contrast, the commercial gelatin sponges presented 230 s and 100 s under the same test condition, respectively. Our data also show that the [email protected] sponge provides a good antibacterial effect on both Escherichia coli and Staphylococcus aureus . This study demonstrated a great application potential using pullulan-based material for massive hemorrhage cases.
研究领域:
生物材料学
·
医学工程
·
创伤护理
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Qingwu Wang,Tianyu Luo,Xiaodong Xu,Qiaoyi Han,Xin Xu,Xingxia Zhang,Xia Liu,Qiang Shi
Irregular hemorrhagic traumas always threaten the health of patients due to uncontrollable bleeding and wound infections. The traditional hemostatic materials show dissatisfactory hemostatic efficiency and antibacterial activity in solving these potential bleeding dangers. Herein, we proposed a kind of composites based on flexible wood membrane (FWM) loaded with chitosan/alginate derivative for accelerating rapid hemostasis and preventing infection. FWM was removed part of hemicellulose and lignin by using NaOH/Na 2 SO 3 mixture to obtain excellent flexibility while retaining the original porous structure, followed by loading silver nanoparticles on the FWM surface to prepare AgNPs-FWM as an antibacterial bio-carrier. Then, AgNPs-FWM was coated with polyoxyethylene stearate-modified chitosan and multi-aldehyde sodium alginate to fabricate the composites of chitosan/alginate/AgNPs-FWM (CSA/AgNPs-FWM) using in-situ Schiff base reaction. Furthermore, in vitro and in vivo experiments showed that the CSA/AgNPs-FWM composites exhibited lower BCI value (2.6 ± 1.3 %), more rapid hemostasis (26 s) and lower blood loss (67.8 mg) than that of the traditional materials. The possible mechanism for the hemostasis process was not only the high blood absorption capacity, but also the synergistic interaction between hydrophobic alkane chains, amino groups, aldehydes, hydroxyl groups and blood cells. Moreover, CSA/AgNPs-FWM showed exceptional superiorities in mechanical properties and antibacterial activity, which endowed composites high potential in hemostasis application for irregular external wound.
研究领域:
生物医学工程
·
材料科学
·
创伤治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2025
·
IF 9.6
Pengcheng Zhang,Chuan Yu,Zhuofan Hu,Yijin Li,Guangming Wan,Keyong Tang,Xialian Fan
Self-expanding hemostatic sponge plays an important role in the control of non-compressible hemorrhage in deep wound. After hemostasis is accomplished, the sponge adheres to the wound via blood clots, posing a considerable challenge in wound debridement. A kind of protocatechualdehyde modified chitosan/sodium alginate composite hemostatic sponge with on-demand removal performance is designed in this study. After absorbing blood, the compression sponge rapidly expands and compresses the damaged blood vessels. The physical compression of the hemostatic sponge and the chemical adhesion of catechol is used to promote rapid hemostasis of the wound. The composite hemostatic sponge demonstrated outstanding hemostasis performance in both mouse liver and rat femoral artery bleeding model. Notably, after complete hemostasis of the rat femoral artery, the composite sponge is rapidly removed from the wound by rinsing it with a suitable concentration of Sodium carbonate (Na 2 CO 3 ) solution. This composite hemostatic sponge featuring the on-demand removal capability demonstrates outstanding application potential for non-compressible hemorrhage in deep wounds and provides a novel way for constructing removable hemostatic sponges.
研究领域:
生物医学工程
·
材料科学
·
创伤急救
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）