Analytical Methods
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2014
·Vol. 6
·期 23
·9198-9201
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IF 1.938
Rui Wang,Jian Wu,Fang Zhang,Liu Wang,Feng Ji
1% (w/v) concentration of pullulan as a cross-priming isothermal amplification (CPA) reaction additive has a great acceleration effect on nucleic acid amplification. Using this property of pullulan, on-point specific detection of nucleic acid by lateral-flow dipstick (LFD) within 20 minutes was achieved with initial DNA template of 750 copies.
研究领域:
分子生物学
·
食品科学
·
植物遗传学
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产品关联
DOI
高影响因子
IEEE SENSORS JOURNAL
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2024
·Vol. 24
·期 5
·5895-5903
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IF 4.3
Pang Peng,Zhang Tao,Zhang Xingxu,Ma Binghe,Zhao Keli,Deng Jinjun,Luo Jian
Wall shear stress and flow direction provide a basis for analyzing the boundary layer conditions, investigating drag reduction mechanisms, and enhancing environmental perception. This work presents novel single-loop and dual-loop hot-film sensors driven by the constant temperature (CT), which are capable of simultaneously measuring wall shear stress and flow direction. Based on the heat transfer and fluid dynamics theory, a mathematical model is developed to analyze the flow directions. The sensors feature multilayer structures, where the numerous leads are concealed and embedded in the insulation layer to enhance their robustness and integration. Utilizing the microelectromechanical system technology, sensor prototypes with single-loop and double-loop are fabricated. In particular, a new process method for accomplishing junction holes in the polyimide (PI) insulation layer is proposed. The sidewall-to-bottom angle of junction holes fabricated through wet etching is ~29.4°. After metal lays are deposited in the junction holes, the upper and bottom surfaces of the insulation layer are able to conduct electricity. Moreover, a testing system consisting of a microchannel and a turbulence generator is established to carry out the experimental verification. Then, the hot-film sensors are tested in the microchannel with a maximum Reynolds number of Red = 8600. Low-frequency turbulence as well as natural transition signals are detected by the hot-film sensors successfully. In the range of wall shear stress from 0 to 14.2 Pa, the accuracies of the dual-loop and single-loop hot-film sensors in perceiving flow directions are better than ±3° and ±6.5°, respectively. This work assists to analyze the boundary layer states, to investigate drag reduction mechanisms, and to enhance environmental perception in flow field.
研究领域:
流体力学
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传感器技术
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环境感知
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微制造技术
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Xing Liu,Wenshan Liang,Hui Zeng,Yiyu Jiang,Yan Li,Min Zhang
The high-speed capillary electrophoresis (HSCE) method is a technique that utilizes a high electric field strength applied through a short capillary to reduce the time required for sample separation. However, the increased electric field strength may result in pronounced Joule heating effects. To address this, we describe a 3D-printed cartridge with integrated contactless conductivity detection (C 4 D) head and a sheath liquid channel. The C 4 D electrodes and Faraday shield layers are fabricated by casting Wood's metal in chambers inside the cartridge. Effective thermostatting of the short capillary is achieved by flowing Fluorinert liquid, which provides better heat dissipation compared to airflow. A HSCE device is created by using the cartridge and a modified slotted-vial array sample-introduction approach. Analytes are introduced through electrokinetic injection. With the help of sheath liquid thermostatting, background electrolyte concentration can be increased to several hundred mM, resulting in improved sample stacking and peak resolutions. Additionally, the baseline signal is flattened. Typical cations such as NH 4 + , K + , Na + , Mg 2+ , Li + , and Ca 2+ can be separated within 22 s with an applied field strength of 1200 V/cm. The limit of detection ranges from 2.5 to 4.6 μM with a relative standard deviation of migration times of 1.1–1.2% ( n = 17). The method has been applied to detect cations in drinking water and black tea leaching for drink safety testing, and to identify explosive anions in paper swabs. Samples can be directly injected without the need for dilution.
研究领域:
分析化学
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生物医学工程
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DOI
PubMed
高影响因子
ANALYTICAL BIOCHEMISTRY
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2020
·
IF 2.877
Fangyuan Zheng,Sufang Li,Sunan Wang,Tao Feng,Zejun Jiang,Jiarong Pan
Adulteration of high-quality meat with their cheaper counterparts can be minimized by rapid and reliable methods for detecting meat species. Here an isothermal cross-primer amplification (CPA) technique combined with colloidal gold nucleic acid test strips (CPA strips) was developed to differentiate cow, sheep, arctic fox , and pig meat. A simple primer design for multiplex differentiation using a universal single-labeled CPA primer system and four detection-level species-specific labeling primers were analyzed by colloidal gold-based test strip assay. Moreover, simultaneous detection of fox and pig meat on a double-test line strip was feasible. The CPA strip assay indicated a lower amounts sensitivity of 0.3 ng DNA when one targeted species was tested and a detection limit of 1% when arctic fox meat was detected in the meat mixtures. Using a minimal set of primers, this study provides a promising tool for detecting the species of different types of meat using a constant temperature amplification technology.
研究领域:
食品科学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Li Li,Cuixin Huang,Zhicheng Li,Yulin Zhao,Jun Liu,Yu Zheng,Rong Cao,Yuting Liao
Sichuan shai vinegar (SSV) is an acidic condiment with a long history and unique flavor. Aging is an important production process that determines the unique flavor of shai vinegar. This study compared the differences in physicochemical properties, volatile flavor, and non-volatile flavor substances between the constant temperature aging and natural aging methods of SSV Cupei . The results showed that the total acid and amino acid nitrogen content in naturally aged vinegar was higher than that in constant temperature aged vinegar. Furthermore, 20 different volatile flavor substances and 65 different non-volatile flavor substances were obtained after 21 and 25 days of constant temperature aging and 1, 3, and 5 years of natural aging SSV samples. This indicates that the temperature environment during natural aging was more moderate, allowing acidic compounds to form and stably exist in the aging environment. Correlation analysis further demonstrated that there were more differentiated non-volatile substances significantly related to the Maillard reaction under constant temperature aging condition and related to antioxidant activity under natural aging condition. The results of this study provide a comprehensive understanding of the characteristics and applications of the two aging methods of SSV and guide strategy for factories in the selection of aging methods.
研究领域:
食品科学
·
发酵工程
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Xuetong Li,Yuan Su,Huashuo Chu,Shuxia Lyu,Jingjing Tian,Wentao Xu
Vibrio parahaemolyticus is a major hidden danger of food safety. To develop a rapid, sensitive and on-site detecting method of Vibrio parahaemolyticus ( V. parahaemolyticus ), a strand replacement primer thermostat phosphate (SRPP) visual sensor was proposed, based on Bst DNA polymerase and pyrophosphatase. The novel strand replacement primer (SRP) facilitates chain substitution and to open a self-folding hairpin by adding region at its 3′ end. Under the action of the SRP, a pair of external primers and two inner primers, target DNA is specifically amplified at 63 °C relies mainly on the hairpin. Many pyrophosphates (PPi) are simultaneously generated as by-products, which can be converted into phosphates (Pi) by pyrophosphatase for phosphomolybdate blue visual detection within 5 min. The proposed biosensor can detect 1.29 × 10 3 copies of V. parahaemolyticus within 35 min.
研究领域:
微生物学
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食品科学
·
生物技术
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Qinbo Jiang,Meiyu Chen,Zeyu Qin,Yang Li,Jiawen Li,Hui Zhang
Thermochromic windows can reduce temperature changes, thereby reducing electricity consumption. Traditional hydrogel-based thermochromic windows suffer from volatilization and freezing, reducing their utility as protective enclosures for display cases. Here, we present a dual-layer organohydrogel system (DG), consisting of thermochromic organohydrogels that provide both heat shielding and heat retention for smart windows. The cononsolvency caused by polyethylene glycol (PEG) is introduced to sharply reduce the thermochromic temperature to below 10 °C for applicability in thermostatically controlled display windows by strengthening hydrogen bonding between mediums and affecting the interaction between copolymer chains and water molecules. PEG imparts antifreeze, liquid retention, and regenerative qualities to DG, enhancing its stability and recyclability. Organohydrogels exhibit over 90 % light transmittance, obstruct 99.17 % of solar heat, and scatter 23.14 % of mid-infrared light. Within 24 h, DG conserves energy at high rates of 37.22 % and 27.13 % in hot and cold settings, respectively. Thus, aims of DG are to maintain a stable temperature within thermostatic display cases and promoting emissions mitigation.
研究领域:
材料科学
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能源效率
·
环境工程
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Yuan Su,Xuetong Li,Longjiao Zhu,Huashuo Chu,Yangzi Zhang,Jingjing Tian,Shuxia Lyu,Wentao Xu
To develop an on-site, thermostatic and rapid sensor for the detection of Vibrio parahaemolyticus (V. parahaemolyticus) , A single cross-priming fluorescence (SCPF) sensor was designed using a 3D nano-nucleic acid hybrid material that termed mesoporous silica nanoparticle/nucleic acid-doped nanoflower (MSN/NA-doped nanoflower). In addition, a portable polymerase chain reaction (PCR) tube fluorescence reader was built. Further analysis of the MSN/NA-doped nanoflower morphology and the enhancement mechanism indicated that the MSNs aggregated into larger nanoclusters by adsorbing single cross-priming amplification (sCPA) components, forming MSNs/NAs-doped nanoflowers, and increasing the local concentrations to enhance sCPA efficiency. Cyclic amplification relied mainly on the self-folding hairpin-like structure of the amplified product that continuously formed, opened, re-formed, and opened again. The target DNA was detected with a detection limit of 2.4 copies/μL.
研究领域:
生物技术
·
分子生物学
·
分析化学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）