PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
·
2025
·
IF 1.9
Zehao Li,Zixuan Wang,Lulu Zhang,Feng Yingjie,Jinchu Yang,Haoliang Li,Weimin Song,Qiuling Wang,Yongfeng Yang,Zhenzhen Huang,Yongming Xu,Yufeng Fu,Meizhou Ding,Gang Fan,Jingnan Ren,Aiqun Yu,Jian Feng,Guijie Li,Linhua Huang,Yujiao Cheng
α-Terpineol, an abundant oxygenated monoterpene compound, shows a varied range of beneficial bioactivities. This study focused on the efficient extraction and purification of α-terpineol from the biotransformation of limonene using Penicillium digitatum DSM 62840 mutant (PdTP1-overexpressed OE2 strain). The α-terpineol was primarily distributed in the supernatant, with minimal association with the biomass. And the optimal extraction was achieved using ethyl acetate as the extractant, which can directly obtain the highest recovery of α-terpineol without sequential extractions. The equilibrium of mass transfer was quickly reached (≤20 s in vortex). Additionally, the purification method of α-terpineol using column chromatography was described, further improving the purity of product to 96.86% in gas chromatography. Structural identification of purified α-terpineol was confirmed using gas chromatography-mass spectrometry, Fourier-transform infrared spectroscopy, and nuclear magnetic resonance. This simplified and efficient strategy for the extraction and purification of α-terpineol not only provides a solid theoretical basis for the final step in the relevant study of microbial synthesis of α-terpineol but also is of great significance for its industrial application.
研究领域:
微生物学
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天然产物化学
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药理学
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DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 1
·263
·
IF 4.2
Shuji Liu,Yue Zhang,Yihui Chen,Yongchang Su,Bei Chen,Yin Wang,Min Xu,Kun Qiao,Shuigen Li,Zhiyu Liu
Protamine is a cationic peptide derived from fish sperm and has several important functional properties: antibacterial properties, acting as a carrier for injectable insulin and as a heparin antagonist, combatting fatigue, etc. Thus, it has been widely used in medicinal applications and food products. CulturedTakifugu flavidusis a type of pufferfish with a delicious taste that is popular in China, and its production is increasing significantly. Therefore, protamine was extracted via acid extraction from the sperm ofTakifugu flavidusand further isolated and purified via sephadex gel chromatography, ion exchange chromatography, and desalination chromatography. Furthermore, the physicochemical properties of protamine were investigated. The results showed that the sperm of the culturedT. flaviduswere non-toxic, and the extracted and purified protamine had high contents of arginine (36.90%) and lysine (27.02%), respectively. The secondary structure of protamine was mainly β-folded and irregularly curled. Additionally, protamine exhibited high thermal stability with a denaturation temperature of 176 °C. This study would provide a theoretical basis for the structural analysis, bioactivity, and resource development of pufferfish protamine and help to promote the development of the pufferfish industry.
研究领域:
生物化学
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医药学
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食品科学
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产品关联
DOI
PubMed
高影响因子
Biomass Conversion and Biorefinery
·
2023
·
IF 4
Weng Di,Tian RongRong,Jin HaiXin,Zha ShengHua,Zhao QingSheng,Zhao Bing
In this study, a method to enrich forsythiaside A (FA) from the ball-milled powder of Forsythia suspensa leaves was established. Forsythiaside A was extracted by heating and stirring, and the extraction process was optimized by response surface methodology using the independent variables of extraction temperature, extraction time, liquid-to-material ratio, ethanol concentration, and pH value of the solution, respectively. Then five types of macroporous resins with different polarities were studied to explain the adsorption mechanism by static adsorption and desorption experiments. Different models were used to study the static adsorption thermodynamics and kinetic process of AB-8 resin on FA extracts, and optimal conditions of the process were optimized. Finally, the antioxidant abilities of purified FA were evaluated. The results showed that the optimum extraction conditions were 2.2 h, 53.4 °C, 45.7 mL/g, 60% ethanol, and pH 6, and the extraction efficiency of forsythiaside A could achieve 120.96 mg/g. The AB-8 macroporous resin was the most suitable resin with high adsorption and desorption rates. It was found that the Temkin model was able to calculate the adsorption parameters accurately. The adsorption kinetic process of the resin was in accordance with the pseudo-second-order kinetic model. The optimal process conditions were as follows: the optimum loading concentration of 5.83 mg/mL, the loading rate of 1 BV/h, and the loading volume of 7.51 BV; in the desorption process, deionized water was used to remove impurities first, and then, 30% ethanol was used with an elution rate of 1 BV/h. The purity of FA increased from 20.52 to 81.58%. The purified FA exhibited superior scavenging ability for DPPH radicals and ABTS radicals. Cytotoxicity experiments proved that it was safe and nontoxic and was capable of promoting cell growth. In conclusion, the present study provides a theoretical basis for industrially extracting and purifying FA on a reliable basis.
研究领域:
植物化学
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药理学
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天然产物研究
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
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2022
·
IF 9.231
Shiyang Zhou,Gangliang Huang,Hualiang Huang
The preparation of purified polysaccharides was described from fresh onions by hot water extraction, protein removal and dialysis treatment. The structure of onion polysaccharide was confirmed by 13 C NMR, DEPT, COSY, HSQC and HMBC. In order to study the structure–activity relationship of onion polysaccharide, the derivatives of onion polysaccharide including acetylated onion polysaccharide and phosphoric onion polysaccharide were prepared. On this basis, the antioxidant activities of onion polysaccharide and its derivatives were tested in vitro . The results showed that onion polysaccharides and its derivatives had a good antioxidant activity, and the activity of phosphorylated polysaccharide was similar to that of V c positive control.
研究领域:
食品科学
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生物化学
✓ 已验证
DOI
PubMed
高影响因子
PHARMACEUTICAL CHEMISTRY JOURNAL
·
2017
·Vol. 51
·期 6
·491-493
·
IF 0.445
Zhu Jian,Jin Zhi-Min
Insoluble camptothecin was converted into the corresponding hydrosoluble analog, camptothecin carboxylate, by using 1,1,3,3-tetramethylguanidine as hydrolyzing agent. The ethanol/K 2 CO 3 /H 2 O aqueous two-phase system was established to extract the hydrosoluble camptothecin carboxylate. Through two-stage extraction from crude camptothecin of 5.4%, purity, final hydrosoluble camptothecin was obtained with a purity of 98.5% at a total yield of 78.4%.
研究领域:
药物化学
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分离工程
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING RESEARCH & DESIGN
·
2022
·
IF 4.119
Mengfei Tian,Chunyu Liang,Naveed Ahmad,Chunying Li,Weihao Wang,Yu Zhang,Zidan Luo,Lequn Qiu,Chunjian Zhao
A reverse micellar microemulsion combined with magnetic solid phase extraction (RMM-MSPE) technology was developed for the extraction and purification of juglone from Juglans mandshurica . Reverse micellar microemulsion (RMM) (Tween 80: n-propanol: n-hexane: 0.05 mol/L KCl aqueous solution = 27:13.5:4.5:55; w/w/w/w) was used to extract juglone based on microwave. The optimum conditions were a pH of 5.6, a liquid-solid ratio of 20:1 (mL/g), temperature of 40 °C, time of 63 s, and power of 400 W. Graphene oxide/nano diamonds/ Fe 3 O 4 (GO/ND/Fe 3 O 4 ) was prepared as adsorbent and applied to purify juglone by magnetic solid-phase extraction (MSPE). The optimum adsorption conditions were time of 14 h, solid to liquid ratio of 30 mg/mL, pH of 5.5 and shaker speed of 200 r/min. Anhydrous methanol provided the best desorption of juglone and the optimum conditions were a desorption time of 16 h, pH of 8, and the solid-liquid ratio of 40 mg/mL. The purity of juglone was 69.08% and the recovery yield was 78.67%. RMM and GO/ND/Fe 3 O 4 could be reused three times and eight times after the first use, respectively. The proposed RMM-MSPE is a green and efficient alternative method for extraction and purification of juglone.
研究领域:
植物化学
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环境科学
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药用植物学
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2020
·
IF 4.244
Chang-Wei Zhang,Ming-Fei Li,Ran Tao,Mi-Jun Peng,Zhi-Hong Wang,Zhi-Wen Qi,Xing-Ying Xue,Cheng-Zhang Wang
Polyprenol is an important active ingredient in Ginkgo biloba leaves (GBL) with great industrial application potential. In this paper, a novel high energy ball milling extraction technology coupled with silver-thiolate material purification method was developed, with which a high purity of GBL polyprenol (GBP) was obtained. Results showed that this method had a high extraction efficiency, and GBP of a purity up to 99.8 % was produced. GBP liposomal gel (GLG) was prepared, and its stability, rheological property, release behavior and antibacterial activity were evaluated. According to the results, GLG had a small particle size and high homogeneity which was desirable for topical application, and it also manifested good physiochemical stability and suitable plastic flow property. In addition, GLG illustrated excellent inhibitory effect against Staphylococcus aureus , Escherichia coli , Candida albicans and Streptococcus pneumoniae , and the minimal inhibitory concentration was 25∼200 μg/mL. The systematic research provided a useful method for the biorefining of GBL.
研究领域:
植物化学
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药剂学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）