Algal Research-Biomass Biofuels and Bioproducts
·
2024
·
IF 4.6
Luqiu Lin,Jianhui Zeng,Chuxuan Zhang,Zhengkang Peng,Xun Gong
This study reports the transformation of three typical heavy metals (As(III), Hg(II), and Pb(II)) during the process for magnetic harvesting and subsequent liquefaction in ethanol of microalgae. The magnetic harvesting process of Chlorella Vulgaris (CV) was simulated using a co-precipitation method, achieving the highest magnetic harvesting ratio of 98.95 % at a pH of 8 and an iron-algae ratio of 0.4 g/g. Then the magnetically harvested CV was subjected to liquefaction in ethanol to explore the speciation and migration of As(III), Hg(II), and Pb(II). The environmental risk assessment of above three heavy metals in the bio-oil and biochar was assessed according to the Risk Assessment Code (RAC). Hg(II) and Pb(II) were found to be effectively stabilized and immobilized in the biochar, while As(III) exhibited a propensity to migrate into the bio-oil and existed mostly in the dangerous speciation such as fraction associated with Fe and Mn oxides and fraction bound to organic matter. It reflects a high environmental risk and necessitates a pre-removal treatment.
研究领域:
环境科学
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生物技术
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2019
·
IF 4.191
Wenjing Wang,Yuan Wu,Huihui Xu,Yuan Shang,Yichen Chen,Miaoer Yan,Zhaohui Li,David R. Walt
The harvest time of a plant usually affects the yield of its metabolites. Polygonum tinctorium ( Polygonum tinctorium Ait., P. tinctorium ) is an important cultivated plant that produces the natural blue dye indigo and the medicinal metabolite indirubin. To explore the mechanism of indigo and indirubin accumulation, the leaves of P. tinctorium at different harvest times, namely July, August, and October, were investigated by metabolite and de novo transcriptome analyses. The results revealed that the indigo and indirubin content varied at different harvest times. Moreover, their contents were significantly higher (p < 0.05) in August than in either July or October. Transcriptome analysis showed different gene expression patterns of P. tinctorium leaves in August. Almost all the identified differentially expressed genes (DEGs) that were responsible for the biosynthesis of indigo and indirubin, including four anthranilate synthase (AS) genes, two indole-3-glycerolphosphate synthase (IGPS) genes, three tryptophan synthase (TS) genes and seven cytochrome P450 (CYP450) genes were expressed at significantly higher levels in August ( p < 0.05), which correlated with the increase of the two compounds. Overall, the results of this study provide useful information for understanding the accumulation mechanism of indigo and indirubin in P. tinctorium , which may promote the development of agricultural breeding and industrial production of P. tinctorium. Moreover, sequence data obtained by de novo transcriptome sequencing of P. tinctorium provides a platform for future molecular biological studies.
研究领域:
植物学
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分子生物学
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产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2024
·
IF 8.1
Zhihong Yin,Liandong Zhu,Xiaowen Shi,Francesco G. Gentili,Yohanes Kristianto Nugroho
Flocculation is one of the promising methods for harvesting microalgae. In this study, a series of grafted cationic chitosan-cyclodextrin composite flocculants (CTS/β-CDs) with different structural properties were prepared from chitosan and cyclodextrin. The results showed that the harvesting efficiency of CTS/β-CD-1 for C. vulgaris reached 99.91%. The chain type of polymeric flocculants with moderate graft chain lengths (L = 10–20) and tighter branched chain density distribution (D = 1.0–1.8) was favorable for C. vulgaris harvesting. The addition of CTS/β-CDs caused the discrete C. vulgaris cells to be flocculated and aggregated, and the reorganization of particles resulted in larger particles after flocculation, improving the dewatering performance of microalgae flocs. CTS/β-CDs had no significant inhibitory effect on the extraction of lipids, proteins and carbohydrates, and did not affect the extraction and quality of fatty acid methyl esters, and the re-cultivation experiments of the harvested water showed that chitosan-based flocculant was able to effectively reduce the cost of microalgae harvesting. Electrostatic interactions and bridging were the main harvesting mechanisms between CTS/β-CD-1 and C vulgaris . E-DLVO modeling suggested that flocculation is mainly a result of electrostatic and acid-base interactions when the distance between particles is small. At higher pH, the harvesting efficiency of CTS/β-CD-1 with C. vulgaris decreased. The study contributes to a better understanding of the structure–activity relationship between the structural characteristics of flocculants and the harvesting performance.
研究领域:
生物技术
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环境科学
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产品关联
DOI
高影响因子
Horticultural Plant Journal
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2017
·Vol. 3
·期 4
·165-171
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IF 0
Yanhui ZHANG,Peiying LIU,Qianqian KONG,Shiyan XING,Xiaojing LIU,Limin SUN
Flavonoids and terpene trilactones, especially, ginkgo flavonglycosides, ginkgolides and bilobalides in leaves of ginkgo trees, need to be studied for effective application of these active components with high medical and health-care values. This study was aimed to provide scientific bases for genealogies selection and harvest season confirmation for Ginkgo biloba . A high-performance liquid chromatographic method (HPLC-ELSD) was developed to determine the contents of terpene trilactone and flavonoid of 36 ancient G. biloba genealogies from 19 provinces in China. The study indicated that the content gradually increased from April to August, and thereafter declined. Analysis of variance indicated that the contents of terpene trilactone, flavonoids, and their respective components had significant difference among 36 genealogies. The cluster analysis showed that No. 72 (Xing'an, Guangxi), No. 58 (Youyang, Chongqing), No. 82 (Rugao, Jiangsu), No. 123 (Huixian, Gansu), No. 99 (Dujun, Guizhou), No. 10 (Tai'an, Shandong) and No. 133 (Mentougou, Beijing) genealogies have higher content of terpene trilactone and flavonoid. These results can help us to select superior variety containing high content of terpene trilactone and flavonoid.
研究领域:
植物化学
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药用植物学
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天然产物化学
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林木遗传育种
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药物代谢
✓ 已验证
DOI
Foods
·
2025
·Vol. 14
·期 7
·1155
·
IF 5.1
Lian He,Yuwen Yi,Hongfeng Jia,Chengjian Xu,Mingfeng Qiao,Xuemei Cai,Sze Ying Leong,Nallammai Singaram,Sook Wah Chan,Hua Peng
Sichuan pepper is known for its unique aroma and tingling, numbing sensation, making it a key ingredient in Sichuan cuisine. This study explored the effect of harvest periods on the quality of Sichuan pepper at five selected harvest periods (LSA (early harvest), LSB, LSC, LSD, and LSE (late harvest)) along the 24 solar terms in the traditional Chinese lunar calendar. Apart from evaluating their physicochemical and volatile profiles, the growth characteristics, polyphenol and flavonoid contents, antioxidant properties, and free amino acid and other nutrient concentrations in these peppers were also analysed. Results showed that the moisture content, weight, and shape of Sichuan peppers improved progressively, peaking at the LSE harvest period. Throughout maturation, the energy content of the Sichuan pepper remained stable. Polyphenols and flavonoids, indicators of antioxidant capacity, increased in later harvest periods. A total of 18 amino acids were detected in Sichuan pepper. Proline was the most abundant amino acid, followed by serine, arginine, and glutamic acid, accounting for 83% of the total amino acids. Based on the taste threshold values of amino acids, a taste activity value (TAV) analysis of the amino acids was conducted. The TAV analysis of arginine and glutamic acid were greater than 1, indicating their significant contribution to the bitterness and umami taste, respectively. Through the principal component analysis of the electronic tongue, it was found that Sichuan pepper picked in late July (LSA stage) had a significant difference from that picked in September (LSD and LSE stages), while the difference in taste characteristics between Sichuan pepper in early September and late September was relatively small. Terpenes were the primary volatile compounds, and the number of compounds increased as the harvest period was delayed. PLS-DA analysis revealed that D-limonene had the highest VIP value, indicating its significant contribution to the overall odour of Sichuan pepper, and thus can serve as an indicator for assessing the maturity of Sichuan pepper. This study offers valuable insights for optimising the harvesting period of Sichuan pepper and serves as a theoretical reference for enhancing the development of the seasoning industry.
研究领域:
食品科学
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植物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
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2019
·
IF 7.015
Rui Huang,Zhiquan Liu,Boyin Yan,Yaqiong Li,Huarui Li,Dongmei Liu,Peng Wang,Fuyi Cui,Wenxin Shi
To tailor low-biofouling membranes remains one of the most problematic issues in filtration for microalgae harvesting. The aim of this study is to develop a high negatively charged and hydrophilic membrane to reduce membrane fouling via bioinspired design and Layer-by-layer (LBL) self-assembly technique for the application in microalgae harvesting. Bioinspired adhesive based on the polydopamine and polyethylenimine (PDA/PEI) composite was first linked onto the neutrally charged PC membrane substrates, then the poly-(styrenesulfonate) (PSS)/poly-(diallyldimethylammonium chloride) (PDADMAC) layers were uniformly assembled by electrostatic attraction. The resultant highly negatively charged and hydrophilic PC membranes exhibit a zeta potential of -66.1 mV and a water contact angle of 26°. Moreover, the modified membranes present a high average flux (133.1 L m 2 h -1 ·bar -1 ) in 1-h microalgae filtration, a high algae retention (~100%) as well as a high flux recovery rate (over 94%). Meanwhile, the resultant membranes exhibit excellent chemical stability in high salty solutions, the low pH solutions and tolerance to long-time running in DI water. The combination of bioinspired construction method and LBL self-assembly technique could be expected to be a universal way to endow neutrally charged materials with highly negative or positive surface charge for various applications.
研究领域:
环境科学
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材料科学
·
生物工程
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Dian Dai,Mingxiang Qv,Dongyang Liu,Chunming Tang,Wei Wang,Qirui Wu,Zhihong Yin,Liandong Zhu
Magnetic harvesting of microalgae depends on the interface interactions between microalgal cells and flocculants, and thus it is important to find a reasonable evaluation method understand these interface interactions. In this effort, we discussed the formation mechanism and pH regulation of microalgae magnetic harvesting via magnetic chitosan (Fe 3 O 4 @SiO 2 -CS) synthesized by a micro-emulsion method. The flocculation performances and mechanisms during the harvesting of microalgae by the novel flocculant were evaluated. The synthesis process and structure were determined by XRD, FT-IR, SEM and XPS, and the results indicated that Fe 3 O 4 @SiO 2 -CS was successfully prepared. The collection efficiency of 96.52 % was achieved for Chlorella vulgaris within 3 min using Fe 3 O 4 @SiO 2 -CS with the Fe 3 O 4 @SiO 2 and CS mass ratio of 1:3 under pH 1.0. Moreover, this study introduced the two-dimension fractal dimension and extended Derjaguin-Landau-Verwey-Overbeek (E-DLVO) model to evaluate the harvesting performances and mechanisms of Fe 3 O 4 @SiO 2 -CS. Experimental results showed that a strong electrostatic interaction was formed under acidic conditions between Fe 3 O 4 @SiO 2 -CS and microalgae cells with D 2 = 1.82 (fractal dimension value). The hydrophobic tryptophan-like amino acid was observed in the slime-extracellular polymeric substances (S-EPS) secreted by microalgae. S-EPS was found to be a significant substance to promote harvesting performance when the system was neutral or alkaline. This work provides deeper insights into microalgae harvesting by Fe 3 O 4 @SiO 2 -CS as well as reasonable mechanisms to illustrate the interaction of Fe 3 O 4 @SiO 2 -CS and microalgae under different pH values.
研究领域:
环境科学
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生物技术
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2018
·
IF 3.997
Yifei Zhu,Zhiguo Cheng,Wenjian Weng,Kui Cheng
This study presents a convenient and versatile way to prepare functionalized composite polydopamine/titanium dioxide (PDA/TiO 2 ) film on polystyrene (PS). First, polystyrene substrate was immersed in dopamine chloride solution, and then collosol containing TiO 2 and water was spun on it, to produce uniform, continuous PDA/TiO 2 composite films. The thickness of film was controllable by adjustment of the spin speed. It was found that the films were strongly adhered on the PS substrate, with peel strength and shear strength of 2.78 MPa and 37.78 MPa, respectively. After 20 min of ultraviolet (365 nm) illumination, over 90% of fibroblasts and 77% of osteoblasts detached from the PDA/TiO 2 composite film. Additionally, the detached cells showed good viability, allowing further culture and applications. This preparation method could be widely applied for cell and cell sheet harvesting directly from PS-based culture wares.
研究领域:
生物材料学
·
细胞工程
·
表面科学
✓ 已验证
DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 10
·1795
·
IF 5.1
Fan Huang,Haijun Wu,Fan Luo,Yingchun Wang,Yulong Ye,Yiyun Gong,Xianlin Ye
The flavor profile of white tea emerges from the natural biochemical composition of its tender leaves, a delicate balance profoundly shaped by seasonal growing conditions and tea cultivars. However, the effects of harvest seasons on biochemical and volatile compounds in white teas in southwestern China have not been fully analyzed at present. This study investigated the sensory characteristics, biochemical components, and volatile compounds of ‘Sanhua1951’ spring white tea (SH-S), ‘Sanhua1951’ autumn white tea (SH-A), ‘Fudingdabai’ spring white tea (FD-S), and ‘Fudingdabai’ autumn white tea (FD-A). The results showed that the sensory quality (appearance, taste, and aroma) scores of spring tea were higher than those of autumn tea. Spring teas exhibited significantly higher epigallocatechin, soluble sugar, and amino acid levels than autumn teas (p< 0.05), whereas autumn teas contained greater contents of epicatechin gallate, catechin, caffeine, and polyphenols (p< 0.05), which were responsible for the differences in taste quality observed between samples with different harvest seasons. A total of 90 volatile compounds in four groups were identified through HS-SPME–GC–MS analysis, and spring white teas contained higher contents of and variability in volatile compounds than autumn white teas. According to the OPLS-DA model, 52 and 57 differential volatile compounds (VIP > 1,p< 0.05, and fold change ≥ 2 or ≤0.5) were identified in SH-S vs. SH-A and FD-S vs. FD-A, including (Z)-linalool oxide, (E)-linalool oxide, styrene, phenylethyl alcohol, (Z)-citral, etc. The odor active value (OAV) results indicated that 30 key differential volatile compounds (OAV > 1) were determined in four groups, among which β-ionone, 5,6-epoxy-β-ionone, linalool, and (E)-linalool oxide exhibited particularly high OAVs and contributed more pekoe aroma and floral sensory characteristics. Notably, (E)-linalool oxide, (Z)-jasmone, and δ-cadinene were identified in each cultivar. These findings suggest their potential as seasonal markers, paving the way for the development of white tea ’Sanhua1951’ and ’Fudingdabai’.
研究领域:
食品科学
·
植物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2019
·
IF 6.669
Yuxi Liu,Wenbiao Jin,Xu Zhou,Song-Fang Han,Renjie Tu,Xiaochi Feng,Paul D. Jensen,Qilin Wang
In this work, a novel flocculation process by using nano-Fe 3 O 4 coated with polyethyleneimine (Fe 3 O 4 @PEI) as magnetic seeds was developed to harvest the microalgae cultivated in urban sewage. Experiment results indicated that the harvest efficiency of Chlorella pyrenoidosa (0.5 g/L) was 98.92 ± 0.41% under the optimal conditions of Fe 3 O 4 @PEI dose of 20 mL/L, flocculation time of 20 min, and stirring speed of 800 rpm (3 min), while that of Scenedesmus obliquus (0.4 g/L) was 98.45 ± 0.35% under a Fe 3 O 4 @PEI dose of 16 mL/L, flocculation time of 15 min, and stirring speed of 730 rpm (3 min). Moreover, the process did not reduce the lipid content of microalgae and quality of biodiesel. After microalgae harvest, Fe 3 O 4 @PEI could be recovered by ultrasonication, re-wrapped with polyethyleneimine and reused to reduce operational cost.
研究领域:
环境科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Cleaner Production
·
2021
·
IF 9.297
Wenqi Qin,Siqi Zhang,Shujuan Yang,Feilin Lin,Qichang Zhou,Houkui Gong,Xinyu Zhao,Furui He,Yang Zhou,Yuhong Feng,Gaobo Yu,Yuanyuan Liu,Jiacheng Li
Microalgae have been known as a promising sustainable resource for bioenergy cleaner production. Herein, a series of highly dispersible novel magnetic quaternary ammonium cationic surfactants (Fe 3 O 4 @DQn, n = 1, 2, 3) for the efficient harvesting of oleaginous Chlorella pyrenoidosa ( C. pyrenoidosa ) have been investigated. The Fe 3 O 4 @DQn capture microalgae process can be divided into a three-step operation (i.e., flocculation , separation, and recycle). For harvesting C. pyrenoidosa , the Fe 3 O 4 @DQn has both a high recovery efficiency (>98.4%) and a short flocculation time (2 min). In addition, the reused Fe 3 O 4 @DQ2 can maintain a high recovery efficiency (92.31%) with five cycling times. Moreover, the N -[3-(Trimethoxysilyl)propyl]ethylenediamine (TMPED) amount and the pH value both have a positive influence on the C. pyrenoidosa harvesting. It is worth noting that the dual effect of quaternary ammonium cationic surfactant, strong positively charged and hydrophobic properties, leads to a higher harvesting efficiency of microalgae. Furthermore, the results of particle size distribution and turbiscan stability index (TSI) show that a higher specific surface area, a smaller polydispersity index and TSI value of the Fe 3 O 4 @DQn can make a more excellent adsorption and dispersion properties. Finally, the Fe 3 O 4 @DQn might provide a promising high cost-performance to harvesting of oleaginous microalgae due to the better economic viability.
研究领域:
生物技术
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Zhihong Yin,Shuangxi Li,Dan Hu,Zhuo Li,Ruoyu Chu,Chenchen Liu,Xinzhu Li,Jiangjun Hu,Liandong Zhu
As an environmentally friendly bioflocculant, chitosan has a great application potential in the field of microalgae harvesting due to its biocompatibility. In this work, three different inorganic clay minerals (kaolin, montmorillonite, and diatomite) were selected to prepare chitosan-clay composite materials (CTS/KAL, CTS/MML and CTS/DTE) as flocculants to harvest microalgae cells. The harvesting capacities of CTS/KAL, CTS/MML and CTS/DTE were accordingly 97.37%, 97.81% and 98.34% for microalgae cells under the dosage of 60 mg/L, microalgae concentration of 0.923 g/L, settling time of 30 min and pH of 10. CTS/DTE showed excellent harvesting performance for microalgae cells, mainly due to the enhancement of charge neutralization, sweeping and bridging effects. Based on the phenomenological theory, the second-order polynomial model was used to analyze the influences of different external factors on the microalgae harvesting efficiency. A reasonable performance relationship between CTS-clays for harvesting of microalgae cells and external factors was established. The analysis of carbohydrates, proteins, and fatty acids of microalgae biomass collected by CTS/KAL, CTS/MML, CTS/DTE and centrifugation showed that the harvesting of CTS-clay did not influence the properties of biomass. The experimental results of water resource recycling and re-cultivation after microalgae harvesting by CTS/KAL, CTS/MML and CTS/DTE exhibited that the growth rates of 50% circulating medium + 50% fresh BG11 culture medium were accordingly 2.512, 2.466 and 2.572 times higher than those of 100% circulating medium. It is concluded that chitosan-clay composite materials are efficient and environmentally friendly flocculants with a great applicable potential in microalgae harvesting.
研究领域:
环境科学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）