SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Ruizhuo Li,Jing Dong,Guojun Wu,Limin Gao,Min Yang
Turbidity interference in measurements can reduce the accuracy of fluorescence detection. Conventional turbidity compensation methods directly establish the relationship between turbidity value and fluorescence but cannot accurately characterize the complex interference of turbidity on fluorescence detection. This paper introduces a novel turbidity compensation technique that separates the interference caused by turbidity particles into scattering intensifying and scattering-absorption attenuating components and corrects them separately. First, the scattering spectrum overlapping with fluorescence is estimated and subtracted from the actual sample spectrum to mitigate the fluorescence intensification caused by scattering. Then, attenuation coefficients at different turbidity intervals are calculated to compensate for fluorescence attenuation. Finally, the two components are combined to obtain the final corrected result. Based on the proposed method, the fluorescence spectra data of Platymonas helgolandica var. tsingtaoensis and Synechococcus elongatus undeod is evaluated. The core problem for comper different turbidity interferences were analyzed. Intensifying and attenuating coefficients based on turbidity values and scattering spectra were determined, ensuring adaptability to known and unknown turbidity conditions. The study results show that the fluorescence variation at different concentrations and turbidity levels are influenced by sample concentration and turbidity, exhibiting nonlinear behavior. The compensation model developed was applied to experimental data, achieving a mean relative error of less than 4% and a satisfactory root-mean-square error, significantly enhancing prediction accuracy. This method offers a straightforward and rapid application to detect a wide range of fluorescent substances.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2020
·
IF 2.369
Taotao Zhan,Junshuai Chen,Xin Li,Qi Zhou,Maogang He,Ying Zhang
The experimental speeds of sound combined with Brillouin frequency shifts of ethyl laurate at temperatures from 294.97 to 572.89 K along six isobaric lines from 0.1 to 10.0 MPa were measured by Rayleigh–Brillouin light-scattering spectroscopy. The expanded relative uncertainty in the reported speeds of sound in ethyl laurate is estimated to be 1.3% (coverage factor k = 2 with a confidence level of 0.95). Within the temperature limits of 303.15–383.15 K and at pressures up to 10.0 MPa, the derived thermoacoustic properties of ethyl laurate including density, specific isobaric heat capacity, isentropic compressibility, isobaric thermal expansivity, isothermal compressibility, the difference in isobaric and isochoric heat capacity, internal pressure, and intermolecular free length were studied. Because of the heavily updated data of density and the speed of sound in the literature, a modified Jacobson’s model for biodiesels was proposed over the temperature range from 293.15 to 343.15 K, which is able to calculate the intermolecular free length of ethyl laurate with higher accuracy compared with Jacobson’s model.
研究领域:
物理化学
·
材料科学
✓ 已验证
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2018
·
IF 2.88
Jinxia Xu,Zhangjun Bai,Fanlin Zu,Fanyong Yan,Junfu Wei,Saihui Zhang,Yunmei Luo
A convenient, highly sensitive and reliable assay for 2,4,6‑trinitrophenol (TNP) and Fe (III) ion (Fe 3+ ) in the dual spectroscopic manner is developed based on novel carbon dots (CDs). The CDs with highly blue emitting fluorescent were easily prepared via the one-step potassium hydroxide-assisted reflux method from dextrin . The as-synthesized CDs exhibited the high crystalline quality, the excellent fluorescence characteristics with a high quantum yield of ~13.1%, and the narrow size distribution with an average diameter of 6.3 ± 0.5 nm. Fluorescence and frequency doubling scattering (FDS) spectra of CDs show the unique changes in the presence of TNP/Fe 3+ by different mechanism. The fluorescence of CDs decreased apparently in the presence of TNP via electron-transfer. Thus, after the experimental conditions were optimized, the linear range for detection TNP is 0–50 μM, the detection limit was 19.1 nM. With the addition of Fe 3+ , the FDS of CDs appeared to be highly sensitive with a quick response to Fe 3+ as a result of the change concentration of the scattering particle. The emission peak for FDS at 450 nm was enhanced under the excitation wavelength at 900 nm. The fluorescence response changes linearly with Fe 3+ concentration in the range of 8–40 μM, the detection limits were determined to be 44.1 nM. The applications of CDs were extended for the detection of TNP, Fe 3+ in real water samples with a high recovery. The results reported here may become the potential tools for the fast response of TNP and Fe 3+ in the analysis of environmental pollutants .
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Water Chemistry and Technology
·
2022
·Vol. 44
·期 6
·482-487
·
IF 0.484
Ruijia Lan,Su Wenbin,Jia Yanan,Li Jitai
Abstract With the increasingly extensive application of persulfate for water treatment, the development of persulfate detection technology has gradually become a research hotspot. Ferrous ions can catalyze peroxydisulphate (PDS) to produce \({\text{SO}}_{4}^{ - }• \) and \(• {\text{OH}}\) , which oxidize excessive KI to form \({\text{I}}_{3}^{ - }\) . Then \({\text{I}}_{3}^{ - }\) and cationic dye (Basic Blue 54) form ion association complex through electrostatic interaction. These complexes aggregate to form particles with the average size of 362 nm. Formation of ion association particles leads to sharp enhancement of resonance scattering intensity of the system. Based on this principle, a new method for the resonance light scattering determination of peroxydisulphate has been developed, and the optimum reaction conditions, influential factors and applications were investigated. Initial solution pH of 4.5, initial Fe 2+ concentration of 3.0 × 10 –6 mol L –1 , KI concentration of 1.0 × 10 –3 mol L –1 , BB54 concentration of 2.0 × 10 –5 mol L –1 , these conditions were selected as the optimized initial concentration for the detection of PDS. Under the optimal reaction conditions, peroxydisulphate concentrations of 2.5 × 10 –7 –1.8 × 10 –5 mol × L –1 were linearly correlated with the reduction of the resonance light scattering intensity with a correlation coefficient of 0.99 and a detection limit of 7.8 × 10 –8 mol L –1 . This method of measuring PDS shows a higher sensitivity than other methods. The interferences of coexisting substances were also investigated. It has been applied to the analysis of peroxydisulphate in water samples. The recovery rates of the spiked samples were in the range between 91.1–106.2% which showed that the method had high accuracy for the analysis of peroxydisulphate.
研究领域:
分析化学
·
环境科学
✓ 已验证
DOI
高影响因子
Nanoscale
·
2014
·Vol. 7
·期 2
·511-517
·
IF 6.739
Tao Xie,Chao Jing,Wei Ma,Zhifeng Ding,Andrew James Gross,Yi-Tao Long
The morphological characteristics of metal nanoparticles, particularly the shape, play an essential role in the optical, physical and chemical properties. In this work, we reported a transverse etching process to investigate the morphological variations of single gold nanorods (GNRs). Dark-field microscopy and Rayleigh scattering spectroscopy were used as complementary technologies to track the transverse etching process. Dark-field imaging with high spatial and temporal resolution could easily monitor the transverse etching process of GNRs in situ and in real time. Interactions between the scattering spectrum and the morphological variations were judiciously calculated within the dipole approximation by the Drude function. The calculated peak shift of GNRs (Δλmax = 17 nm) was obtained via the ratio of the long axis and short axis (aspect ratio) of GNRs from transmission electron microscopy. The average scattering peak shift (Δλmax = 22 nm) from Rayleigh scattering spectroscopy was in good agreement with the calculated peak shift. Monitoring the morphological variations of single GNRs enables us to track the transverse etching of GNRs at arbitrary time. This promises to be a useful method for the study of different nanomaterials and their spectral properties.
研究领域:
纳米材料科学
·
光学
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Qiannan Duan,Zhaoyi Xu,Shourong Zheng,Jiayuan Chen,Yunjin Feng,Luo Run,Jianchao Lee
Determination of complex pollutants often involves many high-cost and laborious operations. Today’s pop machine-learning (ML) technology has exhibited their amazing successes in image recognition, drug designing, disease detection, natural language understanding, etc. ML-driven samples testing will inevitably promote the development of related subjects and fields, but the biggest challenge ahead for this process is how to provide some intelligible and sufficient data for various algorithms. In this work, we present a full strategy for rapid detecting mixed pollutants through the synergistic application of holographic spectrum and convolutional neural network (CNN). The results have shown that a well-trained CNN model could realize quantitative analysis of the mixed pollutants by extracting spectral information of matters, suggesting the strategy’s value in facilitating the study of complex chemical systems.
研究领域:
环境科学
·
化学
·
人工智能
✓ 已验证
DOI
PubMed
高影响因子
ANALYST
·
2011
·Vol. 137
·期 1
·195-201
·
IF 3.913
Jianbo Xiao,Yaru Zhao,Fangfang Mao,Jie Liu,Muxia Wu,Xibin Yu
The effect of a ZnO#ZnS QDs heterojunction (O#SQDs) on the binding affinities of flavonoid glycosides for bovine serum albumin (BSA) was investigated. The fluorescence intensities of BSA decreased remarkably with increasing concentration of O#SQDs. The magnitudes of the binding constants of flavonoid glycosides for BSA in the presence of O#SQDs were in the range of 105–107 L mol−1, and the number of binding sites per BSA (n) was determined as 1.24 ± 0.17. O#SQDs increased the affinities of flavonoid glycosides for BSA by about 2.96% to 114.68% depending on their structures. O#SQDs in blood will enhance the transportation of flavonoid glycosidegs in blood and improve their pharmacology effects. From this point, O#SQDs are a perfect candidate for flavonoid glycosides delivery applications.
研究领域:
生物化学
·
药理学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2016
·
IF 2.758
Congfei Yin,Weidong Xiang,Guangcai Hu,Xie Hu,Weibin Fan,Xipeng Chen,Run Xiang,Xiaojuan Liang
A two-step synthetic method was used to obtain a Pb glass film containing micro-crystalline Ca 2 CuO 3 particles on a K 9 glass substrate. The Ca 2 CuO 3 micro-crystals were prepared for the first time by a high-temperature solid state method. The synthesized films were obtained on K 9 glass substrates by screen-printing and co-sintering techniques, using Pb glass powder and Ca 2 CuO 3 micro-crystals as reagents under different sintering temperatures . Secondly, micro-crystalline Ca 2 CuO 3 Pb-glass films with different thickness were obtained by mechanical polishing for various times. The analysis of the surface structure, purity of the Ca 2 CuO 3 phase and confirmation of the Pb-glass components in the given composites were provided by X-ray powder diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and X-ray photoelectron spectroscopy (XPS). The interactions between phonons and atoms of the micro-crystalline Ca 2 CuO 3 particles embedded in the Pb-glass matrix were characterized by means of Raman scattering spectra. Moreover, the nonlinear optical property of the film was detected by a top-hat Z-scan technique with a laser duration of 190 ns at 532 nm. The results showed that the film polished for 120 min exhibited a large third-order non-linear susceptibility χ (3) of 6.1×10 −12 esu, suggesting its potential application in all-optical switches and integrated electron devices.
研究领域:
材料科学
·
光学工程
·
固体物理
✓ 已验证
DOI
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 89
·73091-73098
·
IF 3.84
Xu Zang,Jingdong Peng,Mingqiong Zhou,Huanjun Peng
A highly selective and sensitive method of high performance liquid chromatography combined with resonance Rayleigh scattering spectra was developed for the detection of six psychotropic drugs, including Doxepin, Promethazine, Imipramine, Amitriptyline, Chlorpromazine and Clomipramine. This method was based on the weak intensity of the RRS of six drugs and its significant enhancement after combined with Erythrosine (Ery) in a pH 4.0–4.7 BR buffer solution. The linear range was between 0.05 and 20 μg mL−1 with correlation coefficients (r) all above 0.9916. The limits of detection (S/N = 3) were in the range of 0.42 and 21.05 ng mL−1. Because the RRS detection wavelength was special (the excitation wavelength was equal to the emission wavelength, λex = λem = 295 nm) and some other substances in human urine do not form ion-association complex with the Ery, no interference from the matrix was observed. The proposed method was successfully applied to real human urine samples analysis.
研究领域:
分析化学
·
医学检验
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2020
·
IF 7.489
Zi Yu Pan,Jun Zhou,Hong Yan Zou,Yuan Fang Li,Peng Fei Gao,Cheng Zhi Huang
Insightful understanding of size-dependent optical signatures and precise regularity of nanosensors is critical for developing applications of plasmonic sensing. This work presents a systematic study on localized surface plasmon resonance (LSPR)-based nanosensors of plasmonic silver nanocubes (AgNCs) with the edge lengths of 59.84 ± 7.97 nm (no. 1 AgNCs), 75.70 ± 9.05 nm (no. 2 AgNCs) and 110.32 ± 14.63 nm (no. 3 AgNCs), respectively. The effects of different sizes on the scattering signatures and refractive index (RI) sensitivities of AgNCs were in situ determined using the multi-model co-localization approach of single AgNC by dark-field microscope (DFM), LSPR spectroscopy and scanning electron microscopy (SEM). The scattering light colour of single AgNC took place bathochromic shift from monocolour to multicolour with the growth of edge length of single AgNC. The LSPR scattering spectra of no. 1 and 2 AgNCs exhibited singlet and singlet with the shoulder peak from quadrupolar resonance mode, respectively. Compared with the scattering signatures of no. 1 and 2 AgNCs, the interesting LSPR effect of plasmon line shape with two distinct peaks was observed on single no. 3 AgNC. In situ studies on the scattering spectral response of single AgNC to the ambient solvents and probing the small-molecule adsorbates on the surface of single silver nanocube reveal that no. 2 AgNC is more suitable as nanosensor due to strong regularity and higher sensitivity. The mechanism involved in optical signatures was elaborated clearly by combining with the experiments and theoretical simulation.
研究领域:
光学
·
材料科学
·
纳米技术
✓ 已验证
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2018
·
IF 4.439
Hua Zhang,Guanhua Li,Shuang Li,Linlin Xu,Yue Tian,Anxin Jiao,Xiangdong Liu,Feng Chen,Ming Chen
Hexagonal boron nitride/gold nanocomposites (h-BN/Au NCs) were fabricated and developed for ultra-sensitive detection of crystal violet (CV) and creatinine molecules using surface enhanced Raman scattering spectroscopy (SERS). The overgrowth of Au nanoparticles was driven by laser-induced photoexcitation of h-BN, which was monitored by absorption spectra. The h-BN/Au NCs with controllable Au compositions (0–1.85%) were characterized using Transmission Electron Microscopy (TEM), Scan Electron Microscopy (SEM), X-ray diffraction (XRD) and X-ray photoelectron spectra (XPS). The microstructural analyses indicated that mono-dispersed Au nanoparticles with size of ∼10 nm can be accreted on h-BN via 30 min synthetic reaction. The comparative SERS results reveal that the h-BN/Au NCs with 1.68% Au composition provide enhanced SERS activity in comparison with other nanosubstrates in this paper. The corresponding enhancement mechanism of h-BN/Au NCs has been illustrated in detail, which can be composed of four contributions in our research. Then, the SERS analyses demonstrated that the detection limit of CV molecules was achieved at femtomole level of ∼10 −15 M, leading to ultrasensitive monitoring of dye pollution. More importantly, well-defined linear relationships were established between SERS signal intensities and logarithmical scale of creatinine concentration (10 −2 –10 −6 M), providing a precise assessment of creatinine in pathological diagnostics.
研究领域:
材料科学
·
分析化学
·
生物医学
✓ 已验证
DOI
高影响因子
TALANTA
·
2018
·
IF 4.244
Ying-Bo Liu,Ting-Ting Zhai,Yan-Yan Liang,Yue-Bo Wang,Xing-Hua Xia
We demonstrated a sensitive method for detection of glutathione (GSH) based on LSPR scattering spectrum using gold core-satellite nanostructure linked by T-Hg 2+ -T base pair . The core-satellite assembly caused coupling between plasmonic nanoparticles , which inducing distinct change of LSPR peak wavelength. As the interaction between Hg 2+ and GSH, the core-satellite nanostructure would be disassembled, which accompanied with spectral blue-shift of the scattering spectrum. By using this method, GSH could be quantitatively detected, and the detection limits can reach to 0.1 µM.
研究领域:
纳米技术
·
分析化学
·
生物传感器
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）