CURRENT MICROBIOLOGY
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2025
·Vol. 83
·期 2
·112
·
IF 2.6
Gao Peng,Lu Xie-Feng,Yao Ling,Xu Yuan-Jia,Zhang Shu-Yi,Liu Guo-Hong,Zhou Shun-Gui
Two novel aerobic, rod-shaped, motile bacterial strains, designated as strains sgz302251 T and sgz5001026 T , were both isolated from paddy soils. 16S rRNA gene sequences analysis revealed that strain sgz302251 T exhibited 98.3% similarity with its closest reference strain Paenibacillus vietnamensis N4 T , while strain sgz5001026 T had the highest similarity of 97.5% to Ectobacillus polymachus KACC 18242 T . The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strains sgz302251 T , sgz5001026 T and their closest reference strains were 86.0% and 21.2%, 84.9% and 22.9%, respectively. The genomic DNA G + C contents of sgz302251 T and sgz5001026 T were 63.4% and 68.8%, respectively. Strain sgz302251 T grew at 20–37 °C (optimum 30 °C), pH 5.0–9.0 (optimum 6.5–7.5) and tolerated 0–1.0% NaCl (optimum 0.5%, w/v), while strain sgz5001026 T grew at 4–45 °C (optimum 37 °C), pH 5.0–9.0 (optimum 6.0) and 0–0.5% NaCl (optimum 0%). The quinone in both strains was MK-7 and major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE) and phosphatidylglycerol (PG). The main fatty acids (> 5%) of strain sgz302251 T were anteiso-C 15:0 , anteiso-C 17:0 , and C 16:0 , whereas strain sgz5001026 T included anteiso-C 15:0 , iso-C 15:0 , iso-C 16:0 and iso-C 14:0 . Based on the above evidence, the proposed names of the two novel species are Paenibacillus praedii sp. nov. (type strain sgz302251 T = MCCC 1K09633 T = KCTC 43808 T ), Ectobacillus soli sp. nov. (type strain sgz5001026 T = MCCC 1K09373 T = KCTC 25903 T ), respectively.
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高影响因子
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
·
2022
·Vol. 72
·期 7
·005455
·
IF 2.689
Zhen Wang,Jun-Hui Cheng,Xiao-Yu Zhang,Fei Xu,Hui-Hui Fu,Chun-Yang Li,Yu-Zhong Zhang,Xiu-Lan Chen,Xiao-Yan Song,Xi-Ying Zhang
A Gram-stain-negative, aerobic, flagellated and rod-shaped bacterium, designated strain SM2107T, was isolated from a deep-sea sediment sample collected from the Southwest Indian Ocean. Strain SM2107Tgrew at 4–40 °C and with 0–10.0 % (w/v) NaCl. It reduced nitrate to nitrite and hydrolysed casein, gelatin, chitin and DNA. The phylogenetic trees based on the 16S rRNA genes and single-copy orthologous clusters showed that strain SM2107T, together withRheinheimera tuosuensis,Rheinheimera perlucidaandArsukibacterium ikkense, formed a separate clade, having the highest similarity to the type strain ofRheinheimera tuosuensis(98.3%). The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol and the major cellular fatty acids were summed feature 8 (C18 : 1ω7cand/or C18 : 1ω6c), C16 : 0, C17 : 1ω8сand summed feature 3 (C16 : 1ω7cand/or C16 : 1ω6c). The only respiratory quinone was Q-8. The genomic DNA G+C content of strain SM2107Twas 48.8 %. The digital DNA–DNA hybridization values between strain SM2107Tand type strains ofRheinheimera tuosuensis,Rheinheimera perlucidaandArsukibacterium ikkensewere 41.16, 37.70 and 31.80 %, while the average amino acid identity values between them were 87.59, 86.76 and 83.64 %, respectively. Based on the polyphasic evidence presented in this study, strain SM2107Twas considered to represent a novel species within the genusArsukibacterium, for which the nameArsukibacterium indicumwas proposed. The type strain is SM2107T(=MCCC M24986T=KCTC 82921T). Moreover, the transfer ofRheinheimera tuosuensisandRheinheimera perlucidato the genusArsukibacteriumasArsukibacterium tuosuensecomb. nov. (type strain TS-T4T=CGMCC 1.12461T=JCM 19264T) andArsukibacterium perlucidumcomb. nov. (type strain BA131T=LMG 23581T=CIP 109200T) is also proposed.
研究领域:
微生物学
·
海洋生物学
·
基因组学
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系统发育学
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高影响因子
CURRENT MICROBIOLOGY
·
2020
·Vol. 78
·期 1
·383-388
·
IF 1.746
Zhou Jie,Chen Jianhao,Ma Jieyu,Xu Ning,Xin Fengxue,Zhang Wenming,Zhang Hao,Dong Weiliang,Jiang Min
A Gram-staining-negative, aerobic, non-motile, rod-shaped bacterium with degradation ability of chitin, designated strain YD-1 T , was isolated from landfill soil sample collected in Wenzhou, Zhejiang province, China. The growth of strain YD-1 T occurred optimally in the tryptone soy broth (TSB) with 1.0% NaCl at pH 7.0–8.0, 30 °C. Ubiquinone-8 (Q-8) was the predominant quinone. The polar lipids of strain YD-1 T consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, five glycolipids and four lipids. The major fatty acids were iso-C 15:0 (30.7%), iso-C 17:1 ω9c (23.2%), iso-C 11:0 (18.9%), iso-C 11:0 3-OH (6.8%) and iso-C 17:0 (5.9%). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain YD-1 T was affiliated to the genus Luteimonas with the highest similarity to Luteimonas marina KCTC 12327 T (97.3%), followed by Luteimonas aquatica DSM 22088 T (96.5%) and Luteimonas composti CCUG 53595 T (96.4%). The genomic DNA G + C content of strain YD-1 T was 71.8 mol%. Average nucleotide identity (ANI) and the digital DNA–DNA hybridizations (dDDH) for draft genomes between strain YD-1 T and Luteimonas marina KCTC 12327 T were 82.7% and 26.1%, respectively. On the basis of genotypic, phenotypic and chemotaxonomic data, strain YD-1 T is considered to represent a novel species to degrade chitin in the genus Luteimonas , for which the name Luteimonas wenzhouensis sp. nov. is proposed, with YD-1 T (= KCTC 72425 T = CCTCC AB 2019153 T ) as the type strain.
研究领域:
微生物学
·
环境科学
·
分子生物学
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高影响因子
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