Nanomaterials
·
2020
·Vol. 10
·期 3
·551
·
IF 4.324
Teng Chen,Haitao Wang,Zhouping Wang,Mingqian Tan
As(III) is a toxic heavy metal which causes serious health problems. Therefore, the development of highly sensitive sensors for As(III) detection is of great significance. Herein, a turn-on luminescence resonance energy transfer (LRET) method based on luminous nanorods was designed for As(III) detection. Biotin-labelled As(III) aptamers were tagged to avidin functionalized luminous nanorods as energy donors, while graphene oxide (GO) acted as the energy acceptor. The adsorption of single-stranded DNA on graphene oxide resulted in the efficient quenching of the luminescence of the nanorods through the LRET process. In the presence of As(III), aptamers bonded to As(III) preferentially and resulted in the formation of aptamer-As(III) complexes. The aptamer-As(III) complexes were rubbed off from the GO surface due to their conformational change, which led to the recovery of the luminescence of the nanorods. A good linear relationship between the luminescence intensity and concentration of As(III) was obtained in the range from 1 to 50 ng·mL−1, with a detection limit of 0.5 ng·mL−1. Furthermore, the developed sensors showed good specificity towards As(III) and proved capable of detecting As(III) in the environment and food samples. The proposed time-resolved sensors provide a promising sensing strategy for the rapid and sensitive detection of As(III).Keywords:As(III);time-resolved luminescence;aptamer;nanorods
研究领域:
环境科学
·
分析化学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Jiahui Luo,Zece Zhu
Fluorescence detection has always suffered from high background fluorescence from real samples such as milk. Therefore, cumbersome pretreatments of samples were necessary to remove the fluorescent substances but led to long processing times and low efficiency. Time-resolved luminescence detection is a powerful technique for eliminating short-lived background fluorescence without additional pretreatments. However, the related instruments are usually equipped with high-speed excitation sources and detectors, which are always bulky and expensive. Herein, we developed a low-cost and miniaturized imaging system for high-throughput time-gated luminescence detection. An UV LED array was used to excite multiple samples, the luminescence of which could be detected by a smartphone simultaneously. An analog circuit was designed to synchronize the LED to the mechanical chopper to eliminate the background signals resulting from scattering and short-lived autofluorescence. Compared to other synchronous circuits based on FPGAs and microcontrollers, this analog circuit required no programming and memory. For the first time, high-throughput time-resolved luminescence detection of tetracycline in milk without any separation or enrichment was achieved by utilizing a smartphone as a camera, and the scattered signals and the background fluorescence were eliminated efficiently. The limit of detection reached as low as 53 nM (∼0.024 ppm), lower than the residue limit set by the European Union. This high-throughput time-gated luminescence detection method can be used for quantitative analysis of many real samples with high background fluorescence.
研究领域:
分析化学
·
食品安全
✓ 已验证
DOI
PubMed
高影响因子
Foods
·
2020
·Vol. 9
·期 12
·1758
·
IF 4.092
Bin Lai,Ruiying Wang,Xiaoting Yu,Haitao Wang,Zhouping Wang,Mingqian Tan
Cadmium contamination is a severe threat to food safety. Therefore, the development of sensitive and selective cadmium detection strategies is urgently required. The elimination of background autofluorescence generated from the food matrix is critical to the optical assay for cadmium detection. Herein, a time-resolved phosphorescence sensor based on an “on-off” strategy was developed for cadmium determination in food samples. The phosphorescence nanoparticles were used as a luminous material to minimize the interference of background autofluorescence. The cadmium-binding aptamer was immobilized onto the magnetic beads and combined with a black hole quencher 1 (BHQ1) with complementary DNA as the target recognition element. With the presence of cadmium, the cadmium-binding aptamer bound to cadmium specifically and resulted in the release of BHQ1. The free BHQ1remained in the solution after magnetic separation and quenched the phosphorescence. The phosphorescence intensity was negatively related to the concentration of cadmium. Under optimal conditions, the time-resolved phosphorescence sensor showed a linear response to cadmium concentration within a range from 0.05 to 5 ng mL−1and with a detection limit of 0.04 ng mL−1. This “on-off” time-resolved phosphorescence sensor was successfully applied for cadmium detection in spring water and clam samples, which provided a rapid and straightforward method.Keywords:aptamer;cadmium;magnetic separation;time-resolved luminescence
研究领域:
分析化学
·
环境科学
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食品科学
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2013
·
IF 3.498
Lin Zhang,Lu Tian,Zhiqiang Ye,Bo Song,Jingli Yuan
By using a visible-light-excited ternary Eu 3+ complex, BHHBCB-Eu 3+ -BPT (BHHBCB: 1,2-bis[4′-(1“,1“,1“,2“,2“,3“,3“-heptafluoro-4″,6″-hexanedion-6″-yl)-benzyl]-4-chlorosulfobenzene; BPT: 2-( N , N -diethylanilin-4-yl)-4,6-bis(pyrazol-1-yl)-1,3,5-triazine), as a luminophore, two kinds of novel visible-light-excited europium materials, the silica-encapsulated BHHBCB-Eu 3+ -BPT (Eu@SiO 2 ) nanoparticles and BHHBCB-Eu 3+ -BPT-conjugated bovine serum albumin (BSA–BHHBCB-Eu 3+ -BPT), were prepared for biolabeling and time-resolved luminescence cell imaging applications. The Eu@SiO 2 nanoparticles, prepared by the copolymerization of 3-aminopropyl(triethoxy)silane–BHHBCB-Eu 3+ -BPT conjugate, free 3-aminopropyl(triethoxy) silane and tetraethyl orthosilicate in a water-in-oil reverse microemulsion , are monodispersed, spherical and uniform in size, and strongly luminescent with an excitation peak at ∼400 nm and a long luminescence lifetime of 346 μs. The BSA–BHHBCB-Eu 3+ -BPT, prepared by covalent binding of BHHBCB-Eu 3+ -BPT to BSA, shows also strong visible-light-excited luminescence with a excitation peak at ∼400 nm and a long luminescence lifetime of 402 μs. The two materials were used for labeling transferrin and folic acid . Using the time-resolved luminescence imaging of living HeLa cells, the cell-surface receptors of transferrin and folic acid were successfully visualized by the prepared biolabels based on the ligand–receptor affinity binding interaction. The results demonstrated the feasibility of the new materials as visible-light-excited biolabels for the time-resolved luminescence cell imaging.
研究领域:
生物化学
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材料科学
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医学影像
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 412
·期 19
·4555-4563
·
IF 3.637
Huang Yukun,Wang Chong,Huo Qiuyu,Song Yaning,Du Gang,Wang Lijun,Yang Xiao,Chen Xianggui
A novel sensitive, competitive, and time-resolved luminescence sensor for the detection of ofloxacin (OFL) was developed in this study. The sensor used OFL-specific aptamer as a recognition molecule and rolling circle amplification (RCA) as a signal amplification tool. In this way, the time-resolved luminescence can not only avoid background noise from sample, but also provide robust luminescence for detection. Besides, the separation and enrichment of target veterinary drug can be conducted assisted by magnetic treatment. Under optimal conditions, the logarithmic correlation between the concentration of OFL and the luminescence intensity was found to be linear in the range of 5 × 10 −11 –5 × 10 −8 mol L −1 ( R 2 = 0.9988), with a detection limit (LOD) of 32.1 pmol L −1 . Furthermore, this method was applied to the determination of OFL in chicken and pork samples, exhibiting good recovery (72.5–100%) and repeatability (RSD < 10.0%). These results confirm that this novel established method has good application potential for the detection of OFL in food samples.
研究领域:
分析化学
·
生物传感器
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食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Coatings
·
2025
·Vol. 15
·期 9
·1012
·
IF 2.8
Shijian Sun,Mengquan Liu,Zheng Li,Junqiang Cheng,Dechuan Li
Hollow dendritic Na4La2(CO3)5crystals doped with Sm3+ions were synthesized with sodium carbonate using a hydrothermal method. The unique lifetime of Sm3+enables the optical measurement of luminescent ion content. The X-ray diffraction spectrum indicates that the nanocrystals maintain structural stability with a hexagonal arrangement, even when the concentration of Sm3+reaches 50 at.%. As the concentration of Sm3+increases, the emission intensity of Na4(La1−xSmx)2(CO3)5first rises and then falls. The maximum emission intensity of the fluorescent powder occurs at a Sm3+concentration of 0.04. Beyond this concentration, concentration quenching takes place, primarily due to electric dipole–dipole interactions. Using an excitation wavelength of 404 nm and monitoring at 596 nm, the fluorescence lifetime of Na4(La1−xSmx)2(CO3)5shows a strong dependence on Sm3+concentration, which can be described by a precise equation. Over the range of Sm3+concentrations from 0.005 to 1, the lifetime decreases from 3.126 ms to 0.023 ms. Therefore, optical monitoring of fluorescent powders is crucial for confirming the composition of coatings used in applications such as solid-state lighting and anti-counterfeiting, by utilizing the relationship between lifetime and doping concentration.
研究领域:
材料科学
·
光学材料
·
发光光谱学
·
固态化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Xin Tong,Zicheng Wang,Lina Zhao,Kai Wang,Yuxin Li
Lanthanide coordination compounds (LnCC), as excellent luminophores, exhibit ultrasensitive responses to the structural details of coordination environments, providing an effective platform for chemical and biomedical detection. Due to the overtones of molecular vibrations in water that cause luminescence quenching, LnCC-based sensors are typically used in heterogeneous systems, where the compounds are dispersed as solid nanoparticles in aqueous solvents. However, this reduces sensor stability, affecting sensitivity and selectivity, and complicates mechanistic analysis. In this work, we developed homogeneous luminescence detection techniques using LnCC and investigated the underlying chemical mechanisms. The Eu-DPA coordination compound was synthesized as a homogeneous detector with good water solubility (140.3 mg/g) and excellent luminescence (92.49% quantum efficiency and >2 ms lifetime at 1 g/L concentration) via multifaceted sonication. Thanks to its homogeneous nature, the sensor achieved high sensitivity, selectivity, and stability for detecting the carcinoid marker 5-hydroxyindoleacetic acid (5-HIAA) in water, plasma, and urine. Eu-DPA exposes numerous pyridine carboxylate oxygen sites that form hydrogen bonds with the amino groups in 5-HIAA. Transient Absorption Spectroscopy (TAS) and Time-Resolved Luminescence Spectroscopy (TRES) further confirm that electrostatic interactions between the hydrogen-bonding sites significantly influence the energy transfer process, as evidenced by changes in luminescence behavior. This work not only addresses the challenge of balancing water solubility and efficient luminescence in lanthanide complexes but also offers new insights into the development of practical biomedical luminescence sensors.
研究领域:
无机化学
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分析化学
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生物无机化学
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传感器
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生物医学检测
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配位化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Yihui Hu,Wenjing Guo,Yubin Ding,Hanjun Cheng,Hui Wei
The detection of heparin (Hep) and its contaminant oversulfated chondroitin sulfate (OSCS) is of great importance in clinics but remains challenging. Here, we report a sensitive and selective time-resolved luminescence (TRL) biosensing system for Hep by modulating the photoluminescence of Tb 3+ with guanine-rich ssDNA and Hep-specific AG73 peptide (RKRLQVQLSIRT). With the developed system, Hep including both unfractionated Hep (UFH) and the low molecular weight Hep (LMWH) has been successfully detected with a satisfactory detection limit. Owing to the highly specific interaction between Hep and AG73 peptide, major interfering substances in Hep detection, such as Hep analogs of chondrotin sulfate (Chs) and hyaluronic acid (HA), did not interfere with Hep detection. The established TRL sensing system was then successfully used for monitoring Hep metabolism in living rats by microdialysis . Moreover, the proposed TRL sensing system was further applied to analyze OSCS contaminant in Hep with heparinases treatment by exploring the inhibition effects of OSCS on the activity of heparinases. As low as 0.002% of OSCS in Hep was identified.
研究领域:
分析化学
·
生物医学工程
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 411
·期 7
·1453-1465
·
IF 3.286
Niazi Sobia,Khan Imran Mahmood,Yan Lv,Khan Muhammad Issa,Mohsin Ali,Duan Nuo,Wu Shijia,Wang Zhouping
A rapid and highly sensitive time-resolved fluorescence (TRF)-based aptasensor for simultaneous recognition of mycotoxins ochratoxin A (OTA) and fumonisin B 1 (FB 1 ) was developed using multi-color, Ln 3+ -doped time-resolved fluorescence nanoparticles (TRF-NPs) (NaYF 4 : Ce, Tb and NH 2 -Eu/DPA@SiO 2 NPs) coupled with complementary strand DNA (cDNA) as luminescence probe and aptamers-conjugated amine-functionalized Fe 3 O 4 magnetic nanoparticles (MNPs) act as a capture probe. Under the optimized conditions, the time-resolved fluorescence intensities at 544 and 618 nm corresponded with Tb 3+ and Eu 3+ , respectively, were used to measure FB 1 ( Y = 19,177.1 + (− 12,054.4) x , R 2 = 0.9917) and OTA ( Y = 4138.8 + (− 11,182.6) x , R 2 = 0.9924), respectively. The limits of detection (LODs) for FB 1 and OTA were 0.019 pg mL −1 and 0.015 pg mL −1 , respectively, which were much lower than previously described methods for simultaneous recognition of mycotoxins OTA and FB 1 while detection range varied from 0.0001–0.5 ng mL −1 . This aptasensor was effectively applied to quantity FB 1 and OTA in maize samples and results were compared with ELISA method. This is the first reported time-resolved fluorescence (TRF)-based aptasensor to detect two agriculturally important toxins in the maize. The developed aptasensor has potential to be used for detection of toxins in food safety fields.
Graphical abstract
研究领域:
食品安全
·
分析化学
·
纳米技术
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED MATERIALS
·
2025
·
IF 26.8
Mingxin Zhou,Panlai Li,Xiaoxue Huo,Xue Meng,Hao Suo,Guodong Zhang,Xiaojun Wang,Zhijun Wang
Multimodal luminescence materials exhibiting multiple stimulus responses are highly favored in optical anti-counterfeiting and information encryption applications. However, this static luminescence under fixed stimuli remains vulnerable to replication. The development of dynamic multicolor luminescent materials offers an effective solution, yet integrating multidimensional dynamic luminescence within a single material remains challenge. Here, this work introduces Tb 3+ capture centers into the self-activated luminescent host MgGa 2 O 4 -featuring an alternating layered structure and abundant defects-to construct efficient energy transfer channels. This design enables not only static multicolor luminescence dependent on concentration and interplanar spacing, but also, for the first time, stable dynamic multicolor luminescence modulated by four independent dimensions: excitation wavelength, time, temperature, and pressure. In particular, the time domain reveals dynamic photoluminescence with tunable evolution rates, as well as visible–near-infrared dual-band persistent luminescence. These unique optical properties provide strong potential for advanced anti-counterfeiting and visual temperature/stress sensing. Moreover, this work proposes a 4D coupled dynamic encryption system that integrates self-destruction protection and memory fault-tolerance, thereby greatly reducing the risk of information leakage. Combined experimental and theoretical analyses further elucidate the underlying mechanisms, opening new avenues for the design of multidimensional dynamic multicolor luminescent materials.
研究领域:
材料科学
·
光学材料
·
发光与光子学
·
防伪技术
·
信息安全
·
固态化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INORGANICA CHIMICA ACTA
·
2013
·
IF 1.687
Yan-Jing Gu,Bing Yan
The synthesis of organics 9-hydroxyphenalenone (HPO), 2-methyl-9-hydroxyphenalenone (MHPO), 6-hydroxybenz[de]anthracen-7-one (HBAO), 5-amino-1,10-phenanthroline (phen-NH 2 ) and its functionalized mesoporous MCM-41 by covalent band are reported, as well as those of Europium (III) hybrid mesoporous materials, EuL 3 phen-MCM-41 (L = HPO, MHPO, HBAO). The photoluminescence and microstructural properties are characterized, and the XRD and BET results revealed that all of these hybrid materials have uniformity in the mesostructure. It is worth noting that the excitation spectra of these hybrid materials have a broad absorption, which occupies from UV to visible region (250–475 nm). Upon ligand-mediated excitation with the visible light, the low efficient energy transfer exhibit between the organic ligands and europium(III) ion under visible excitation, but the maximum wavelength (456 nm/457 nm/451 nm) located at blue light region, which is in consistent with the blue LED light, then might be a feasible alternative in producing time-resolved luminescence under LED-excitation.
研究领域:
材料科学
·
化学
·
光电技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）