CHEMICAL ENGINEERING JOURNAL
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2026
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IF 12.5
Hongyang Liu,Tiancheng Gao,Jian Zhang,Xin Cheng,Zheng Xing,Lina Li,Haina Bai
Constructed MOF-in-LDHs to regulate morphology and anchor active sites. • Ternary metal redox cycles coupled with ETP boosted PMS activation. • Ion resistance and cycling stability enable continuous-flow application. Although the development of catalysts with high activity and stability is still a major problem, advanced oxidation processes based on peroxymonosulfate (PMS-AOPs) show promise for the degradation of antibiotics. In this work, FeCu-LDH with an initial fish-scale embroidered spherical morphology was reconstructed during ZIF-67 growth, forming a unique 3D structure with ZIF-67 nanoparticles confined on lamellar substrates. This structure not only provides ideal anchoring sites for ZIF-67 but also alleviates its self-agglomeration and metal ion leaching via spatial confinement. Under optimized conditions, 2 mg of the catalyst achieved 95.12% norfloxacin (NOR) removal within 30 min; positively charged LDHs stabilized negatively charged PMS, while ZIF-67 Co sites triggered the continual production of reactive oxygen species (ROS). DFT calculations showed ZIF-67/FeCu-LDH efficiently activates PMS by enhancing adsorption and interfacial electron transfer. A continuous-flow reactor with this catalyst maintained >90% NOR removal for 8 h, showing good practical potential. Quenching experiments and EPR confirmed SO 4 ·- , ·OH, ·O 2 − and 1 O 2 generation; electrochemical tests verified the participation of type III electron transfer pathway (ETP(III)), with radical-non-radical coupling promoting NOR degradation. Mechanistically, FeCu-LDH lamellar confinement enhances Fe/Cu/Co redox cycles and accelerates electron transfer, supplying sufficient electrons for PMS activation. This study proposes a potential method for designing MOF-in-LDHs composites, providing a reference for the treatment of water contaminated by antibiotics.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Chenyang Wang,Cuiyu Huang,Licheng Yu,Xiwen He,Langxing Chen,Yukui Zhang
In this work, a fluorescent sensor based on dual-emissive RhB/COF@ZIF-90 composite was fabricated for ratiometric detection of tetracycline (TC) residues in foods. To mitigate the aggregation-caused quenching (ACQ) effect of TAPB-DMTA COF, ZIF-90 on TAPB-DMTA COF composite was fabricated by an in situ growth strategy. Rhodamine B (RhB) was subsequently encapsulated within the ZIF-90 framework to construct a dual-emission fluorescent probe. When TC was present, the fluorescence signal at 460, 525 and 570 nm increased linearly with the amount of TC. The I 570 /I 460 and I 525 /I 570 ratio signals were demonstrated in the two linear ranges of 5–100 nM and 0.1–20 μM concentrations of TC, respectively. This multi-signal sensor exhibited a low detection limit of 0.29 nM, enabling highly sensitive detection of TC. Furthermore, this work paves the way for developing other MOF/COF hybrids as multi-emission fluorescent sensors for application in antibiotic sensing in foods.
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JOURNAL OF MOLECULAR BIOLOGY
·
2025
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IF 4.5
Xiangrong Li,Xianfei Liu,Jingjing Zhao,Yujie Sun,Yuxin Liang,Qiongya Hu
The combination of lysozyme with antibiotics has emerged as a promising strategy to combat bacterial resistance; however, the lack of mechanistic understanding regarding their molecular-level interactions remains a critical knowledge gap, hindering the rational design of optimized synergistic therapies. Through integrated spectroscopic techniques (FTIR, CD, UV-vis, fluorescence), thermodynamic analyses, molecular docking, and 200-ns molecular dynamics simulation, we established that doxycycline/tigecycline bind lysozyme via moderate-affinity hydrophobic interactions ( K a ∼10 4 L mol -1 ) but with distinct binding patterns-tigecycline forms additional hydrogen bonds through its glycylamino side chain, yielding higher stability and lower free drug fraction ( f u ) compare to doxycycline. Additionally, doxycycline and tigecycline have a relatively mild effect on lysozyme conformation, but doxycycline has a greater impact on lysozyme conformation than tigecycline. Key findings reveal a paradoxical efficacy relationship: while doxycycline exhibits stronger intrinsic antibacterial activity, the lysozyme-tigecycline complex demonstrates synergistic enhancement (particularly against E. coli ) through stable binding-mediated protection and optimized release kinetics, whereas lysozyme-doxycycline’s weaker binding leads to premature release and reduced activity. These results fundamentally advance protein-drug interaction paradigms by demonstrating that optimal therapeutic outcomes require balanced binding stability, with broader implications for designing lysozyme-based delivery systems where moderately strong interactions enable synergistic effects while preserving structural integrity, offering a template for developing combination therapies against antimicrobial resistance.
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PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
·
2026
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IF 7.8
Qihong Cai,Xiaodan Zheng,Sujun Fang,Qifeng Zou,Hang Zhu,Zhima Yangcuo,Yajuan Zhang,Yong Lin,Chunyan Lian,Shiqi Zhang
The weakly fluorescent of chlortetracycline hydrochloride (CTC) and tetracycline hydrochloride (TC) were alkalized under sodium hydroxide (NaOH) to produce products with strong fluorescence. When the two products were coated with cetyltrimethyl ammonium bromide (CTAB) micelles, the fluorescence intensity was further enhanced, which greatly improved the detection sensitivity. Since the conventional fluorescence spectra of CTC and TC alkaline hydrolysis products-CTAB overlapped seriously, synchronous fluorescence spectra scanning were performed at the wavelength difference (Δλ) was 43 nm to simplify the spectra. Then the two synchronous fluorescence spectra were treated with the first derivative. When λ= 350.6 nm, CTC was measured, here the relative fluorescence intensity of TC was 0. Similarly, so did TC at 360.4 nm. The zero-crossing determination was skillfully utilized to eliminate the mutual interference between the two drugs. Moreover, the fluorescence intensity of water sample background was suppressed to nearly 0 at the zero-crossing. The result showed that detection limit of CTC and TC were 0.2593 ng·mL −1 and 0.2199 ng·mL −1 . Recoveries of CTC and TC in water were 86.42 %-99.17 % and 90.56 %-97.79 %, respectively. The new first derivative synchronous fluorescence spectrometry had the merits of speed, simplicity, sensitive, good selectivity and high accuracy, and it was also environmentally friendly.
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JOURNAL OF HAZARDOUS MATERIALS
·
2023
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IF 13.6
Shunlan Li,Dongdong Chen,Ziqing Liu,Shipin Tao,Tianyi Zhang,Yiwen Chen,Lingzhi Bao,Jie Ma,Yuee Huang,Shengmin Xu,Lijun Wu,Shaopeng Chen
Antibiotic abuse is the main reason for the drug resistance of pathogenic bacteria, posing a potential health risk. Antibiotic surveillance is critical for preventing antibiotic contamination. This study aimed to develop a sensitive and broad-spectrum whole-cell biosensor for tetracycline antibiotics (TCs) detection. Wild-type TCs-responsive biosensor was constructed by introducing a tetracycline operon into a sfGFP reporter plasmid. Using error-prone PCR, mutation libraries containing approximately 10 7 variants of the tetracycline repressor (TetR) gene were generated. The tigecycline-senstive mutants were isolated using high-throughput flow cytometric sorting. After 2 rounds of directed evolution, a mutant epS2–22 of TerR was isolated and assembled as a TCs biosensor. The epS2–22 biosensor was more sensitive and broad-spectrum than the wild-type biosensors. The detection limits of the epS2–22 biosensor for seven TCs were 4- to 62-fold lower than the wild-type biosensor (no response to tigecycline). Meanwhile, the epS2–22 biosensor had a shorter detection time and a stronger signal output than the wild type. In addition, the evolved epS2–22 biosensor showed excellent performance in detecting low traces of TCs in environmental water. These results suggest that directed evolution is a powerful tool for developing high-performance whole-cell biosensors, and the evolved epS2–22 biosensors have the potential for wider applications in real-world TCs detection.
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BIORESOURCE TECHNOLOGY
·
2022
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IF 11.889
Xinyi Liao,Chen Chen,Zhijie Liang,Zhiwei Zhao,Fuyi Cui
In this study, MnCl 2 -impregnated biomass was oxygen-limited pyrolyzed to produce manganese oxide-loaded biochar (MBC), its adsorption behaviors and influencing factors on tetracycline (TTC), norfloxacin (NOR), and sulfamethoxazole (SMX) were systematically investigated. Three antibiotics exhibited enhanced adsorption behavior on MBC, with maximum adsorption capacity as accurately described by Sips isotherm: TTC (534 mg/g) > NOR (67 mg/g) > SMX (28 mg/g). Hydrogen bonding, n/π-π interactions, electrostatic interaction, surface coordination, and hydrophobic interaction are the major mechanisms for the improved adsorption. Manganese oxide particles on MBC promoted surface coordination and hydrogen bonding. Antibiotic molecules with more hydroxyl oxygen-containing functional groups are more susceptible to migrate to biochar surfaces and to be adhered. Moreover, the quantitative structure–property relationship (QSPR) model was constructed and revealed that hydrogen bonding and π-π interactions were crucial for tetracycline antibiotics selective adsorption. Hence, MBC was a prospective adsorbent with promising applications for antibiotic removal in sewage processing.
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Nature Communications
·
2022
·Vol. 13
·期 1
·1-17
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IF 17.694
Wang Xiaojuan,Xu Xiaoling,Zhang Shaojun,Chen Na,Sun Yunfeng,Ma Kuifen,Hong Dongsheng,Li Lu,Du Yongzhong,Lu Xiaoyang,Jiang Saiping
Tigecycline is regarded as the last line of defense to combat multidrug-resistant Klebsiella pneumoniae . However, increasing utilization has led to rising drug resistance and treatment failure. Here, we design a D-alpha tocopheryl polyethylene glycol succinate-modified and S-thanatin peptide-functionalized nanorods based on calcium phosphate nanoparticles for tigecycline delivery and pneumonia therapy caused by tigecycline-resistant Klebsiella pneumoniae . After incubation with bacteria, the fabricated nanorods can enhance tigecycline accumulation in bacteria via the inhibitory effect on efflux pumps exerted by D-alpha tocopheryl polyethylene glycol succinate and the targeting capacity of S-thanatin to bacteria. The synergistic antibacterial capacity between S-thanatin and tigecycline further enhances the antibacterial activity of nanorods, thus overcoming the tigecycline resistance of Klebsiella pneumoniae . After intravenous injection, nanorods significantly reduces the counts of white blood cells and neutrophils, decreases bacterial colonies, and ameliorates neutrophil infiltration events, thereby largely increasing the survival rate of mice with pneumonia. These findings may provide a therapeutic strategy for infections caused by drug-resistant bacteria.
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Computational Intelligence and Neuroscience
·
2022
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IF 3.12
Guiqiu Li,Ying Wei,Yan Guo,Hui Gong,Jie Lian,Guangjian Xu,Bing Bai,Zhijian Yu,Qiwen Deng
This study aimed to evaluate the activity, resistance, clonality of MIC distribution, and the correlation between virulence and resistance genes and biofilm formation of omadacycline (OMC) in clinics for Streptococcus agalactiae isolates from China. 162 isolates were collected retrospectively in China. The S. agalactiae were collected from the body’s cervical secretions, wound secretions, ear swabs, secretions, semen, venous blood, cerebrospinal fluid, pee, etc. The MIC of OMC against S. agalactiae was determined by broth microdilution. The inhibition zone diameters of OMC and other common antibiotics were measured using filter paper. D-test was performed to determine the phenotype of cross resistance between erythromycin and clindamycin. In Multilocus sequence typing (MLST), some commonly-detected resistance genes and virulence gene of these S. agalactiae isolates were investigated using polymerase chain reaction (PCR). Biofilms were detected by crystal violet staining. Our data demonstrated the correalation of the biofilm formation and OMA antimicrobial susceptibility of S.agalactiae clinical isolates with the carrier of virulence gene scpB. Conclusively, OMC exhibits the robust antimcirobial activity against clinical S. agalactiae isolates from China compared with DOX or MIN, and the carrier of the virulence gene scpB might correlate with the biofilm formation in OMC-resistant S. agalactiae.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Rouying Cai,Chenfang Miao,Liang Zhang,Yi Zhou,Yuebin Liu,Chen Zheng,Wendi Han,Zhengjun Huang,Xin Zhou,Shaohuang Weng
Tigecycline (TIGE) plays a significant role in the management of complex and serious bacterial infection. The monitoring of TIGE content in plasma and the further application in pharmacokinetic (PK) study is important for clinical medicine, however, the facile and effective strategy is limited. Herein, a sensitive, accurate and facile method for TIGE detection was developed by fluorescence with F, N codoping carbon dots (F, N-CDs) as probe. F, N-CDs exhibited stable maximum emission fluorescence at 500 nm and large Stokes shift of 135 nm. Such property help to weaken the possible self-absorption of biological system and the spectral overlap of fluorophore . Thus, using F, N-CDs as probe, the method exhibited accuracy and similarity of the analytical performance of TIGE detection in human plasma and rat plasma. The validated method was successfully applied to the PK research of TIGE in rat after intravenous injection at three dose levels of 1 μg/g, 2 μg/g, and 4 μg/g at different time-points. The obtained PK parameters of TIGE using this method were basically consistent with current clinical practice. The findings demonstrated the application ability of F, N-CDs for PK study of TIGE ascribed to the clinical need of simple operation, accuracy and rapidity.
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MOLECULES
·
2023
·Vol. 29
·期 1
·173
·
IF 4.6
Lingling Rong,Ligui Wu,Tiao Zhang,Cui Hu,Haihui Tang,Hongcheng Pan,Xiaoming Zou
To improve the adsorption efficiency of pollutants by biochar, preparing graphene-like biochar (GBC) or nitrogen-doped biochar are two commonly used methods. However, the difference in the nitrogen doping (N-doping) effects upon the adsorption of pollutants by pristine biochar (PBC) and GBC, as well as the underlying mechanisms, are still unclear. Take the tetracycline (TC) as an example, the present study analyzed the characteristics of the adsorption of TCs on biochars (PBC, GBC, N-PBC, N-GBC), and significant differences in the effects of N-doping on the adsorption of TCs by PBC and GBC were consistently observed at different solution properties. Specifically, N-doping had varied effects on the adsorption performance of PBC, whereas it uniformly improved the adsorption performance of GBC. To interpret the phenomenon, the N-doping upon the adsorption was revealed by the QSAR model, which indicated that the pore filling (VM) and the interactions between TCs with biochars (Ead-v) were found to be the most important two factors. Furthermore, the density functional theory (DFT) results demonstrated that N-doping slightly affects biochar’s chemical reactivity. The van der Waals (vdWs) and electrostatic interactions are the main forces for TCs-biochars interactions. Moreover, N-doping mostly strengthened the electrostatic interactions of TCs-biochars, but the vdWs interactions of most samples remained largely unaffected. Overall, the revealed mechanism of N-doping on TCs adsorption by biochars will enhance our knowledge of antibiotic pollution remediation.
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Emerging Microbes & Infections
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2018
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IF 6.032
Jin-xin Zheng,Zhi-wei Lin,Xiang Sun,Wei-hong Lin,Zhong Chen,Yang Wu,Guo-bin Qi,Qi-wen Deng,Di Qu,Zhi-jian Yu
This study investigated the characteristics and mechanisms of eravacycline resistance and heteroresistance in clinical Klebsiella pneumoniae isolates. A total of 393 clinical K. pneumoniae isolates were collected and subjected to eravacycline and tigecycline MIC determinations using the agar dilution method. Eravacycline heteroresistance was assessed by a population analysis profile (PAP). The expression levels of efflux pumps and their regulators were determined by quantitative reverse-transcription PCR (qRT-PCR). This study identified 67 eravacycline-nonsusceptible isolates; among the extended-spectrum β-lactamase (ESBL)-positive isolates, eravacycline-nonsusceptible isolates were detected more frequently than tigecycline-nonsusceptible isolates (21.7% vs. 9.4%, p = 0.001). The study sample was observed to include 20 K. pneumoniae isolates with eravacycline heteroresistance. Compared to the reference strain, oqxA or oqxB overexpression was observed in nine eravacycline-nonsusceptible isolates (range, 35.64–309.02-fold) and 13 eravacycline-heteroresistant isolates (8.42–296.34-fold). The overexpression of macA or macB was detected in 12 eravacycline-heteroresistant isolates (3.23–28.35-fold). Overexpression of the efflux pump regulator gene ramA was observed in 11 eravacycline-nonsusceptible isolates (3.33–94.05-fold) and 18 eravacycline-heteroresistant isolates (3.89–571.70-fold). The eravacycline MICs were increased by one–fourfold by overexpression of oqxAB or macAB in three eravacycline-sensitive isolates. In conclusion, the overexpression of OqxAB and MacAB efflux pumps and the transcriptional regulator RamA were suggested to be involved in K. pneumoniae eravacycline resistance and heteroresistance.
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Advanced Healthcare Materials
·
2024
·
IF 10
Yuwei Zheng,Jiaying Chi,Jiayu Ou,Ling Jiang,Liqing Wang,Rui Luo,Yilang Yan,Zejun Xu,Tingting Peng,Jianfeng Cai,Chuanbin Wu,Peng Teng,Guilan Quan,Chao Lu
The development of narrow-spectrum antimicrobial agents is paramount for swiftly eradicating pathogenic bacteria, mitigating the onset of drug resistance, and preserving the homeostasis of bacterial microbiota in tissues. Owing to the limited affinity between the hydrophobic lipid bilayer interior of bacterial cells and most hydrophilic, polar peptides, the construction of a distinctive class of four-armed host-defense peptides/peptidomimetics (HDPs) is proposed with enhanced specificity and membrane perturbation capability against Pseudomonas aeruginosa by incorporating imidazole groups. These groups demonstrate substantial affinity for unsaturated phospholipids, which are predominantly expressed in the cell membrane of P. aeruginosa , thereby enabling HDPs to exhibit narrow-spectrum activity against this bacterium. Computational simulations and experimental investigations have corroborated that the imidazole-rich, four-armed peptidomimetics exhibit notable selectivity toward bacteria over mammalian cells. Among them, 4H10, characterized by its abundant and densely distributed imidazole groups, exhibits impressive activity against various clinically isolated P. aeruginosa strains. Moreover, 4H10 has demonstrated potential as an antibiotic adjuvant, enhancing doxycycline accumulation and exerting effects on intracellular targets by efficiently disrupting bacterial cell membranes. Consequently, the hydrogel composed of 4H10 and doxycycline emerged as a promising topical agent, significantly diminishing the skin P. aeruginosa burden by 97.1% within 2 days while inducing minimal local and systemic toxicity.
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