ELECTROCHIMICA ACTA
·
2026
·
IF 5.8
Longying Zhao,Jiayu Chen,Donglin Xiang,Mierban Dawuti,Laping He,Yuangen Wu,Han Tao
Residual organophosphorus pesticides (OPs) in food pose a severe threat to public health, necessitating simple and sensitive on-site analytical methods. In this study, a signal-off electrochemical sensing platform for OPs was constructed based on the OP-induced inhibition of silver deposition triggered by red kidney bean esterase (RKBE), coupled with the signal amplification of a CuNi dual-atom catalyst (CuNi-DAC). RKBE catalyzes the hydrolysis of α-naphthyl acetate to produce α-naphthol, which reduces Ag⁺ to Ag 0 deposited on the electrode. The electroactive Ag 0 undergoes oxidation to generate a prominent oxidation current, providing a sensitive sensing signal for detection. CuNi-DAC modification of the electrode accelerates charge transfer, provides more active sites, and effectively amplifies the sensing signal. Upon exposure to OPs, the activity of RKBE is inhibited, thereby suppressing the production of α-naphthol. This leads to a diminished Ag 0 generation and subsequent a weakened oxidation current. The decrease degree of current signal shows a positive correlation with pesticide concentration. Under optimized conditions, the fabricated sensing platform exhibits a good linear response range of 10 pmol/L-1000 pmol/L for fenthion with a low detection limit of 5.4 pmol/L. Spiked recoveries in cabbage and apple samples ranged from 93.3% to 108.5%, validating the sensing platform's practicality and reliability. This work offers a facile and cost-effective electrochemical sensing approach for on-site screening of OPs.
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.1
Jiatong Li,Shiyu Ou,Yaxuan Luo,Qing Han,Yongxin Li,Hui Huang
A new cerium-based nanozyme (Bpy Ce) with high organophosphorus hydrolase-like activity was simply prepared. A sensing platform free of natural enzymes was constructed for the selective detection of organophosphorus pesticides (OPs) in juice drinks and fruits, based on both absorbance measurements and smartphone-assisted RGB value extraction. The synthesis method of Bpy-Ce was simple, structurally stable, and had good organophosphorus hydrolase-like activity. Fenitrothion was hydrolyzed to yellow 3-methyl-p-nitrophenol through hydrolysis of the P O bond. This hydrolysis process can be directly judged visually. Therefore, not only were the absorbance values used to determine the yellow hydrolyzed product of fenitrothion, but also the color of the product in the picture was preserved through smartphone-assisted methods. The RGB values of the colors in the image were extracted, and the R, G, and B values were added. These methods can detect the corresponding concentration of fenitrothion in juice drinks. The colorimetric sensing platform exhibited excellent performance for point-of-care detection (POCT) of real samples. And the detection limit of Bpy-Ce nanozyme for fenitrothion has reached 0.022 μg/mL. Free of natural enzymes fenitrothion sensing platform exhibited selective and robust anti-interference capability, providing a new strategy for selective point-of-care detection of OPs.
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MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 4
·252
·
IF 5.4
Shi Fan,Hu Xiaojuan,Han Xiao,Li Juntong,Li Xiaojiao,Mohan Brij,Sun Wei
A freestanding manganese oxide nanoparticle–decorated laser-induced graphene electrode (MnOx-LIGE) for sensitive electrochemical detection of the organophosphate insecticide fenitrothion (FT) is reported. The electrode is fabricated via a one-step laser-engraving process on MnCl₂-doped polyimide films, enabling the simultaneous formation of porous graphene and in situ decoration with MnOx nanoparticles. Structural characterization confirms a three-dimensional porous graphene network uniformly decorated with MnOx nanoparticles, providing abundant active sites and accelerated electron transfer. Benefiting from this architecture, the MnOx-LIGE exhibits a wide linear detection range from 100.0 nmol/L to 250.0 µmol/L with a low detection limit of 13.66 nmol/L. Integrated with a portable electrochemical workstation, the sensor demonstrates excellent selectivity and stability. It achieves reliable recoveries of 102.4–104.2% in mango samples, highlighting its potential for rapid on-site monitoring of pesticide residues.
Section
Graphical
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JOURNAL OF HAZARDOUS MATERIALS
·
2026
·
IF 10.6
Yuqing Cheng,Tian Tian,Donghui Song,Yu Ma,Zhichun Bi,Xinjian Zhang,Ling Zhang,Yongxin Li,Hui Huang
Sulfonylurea herbicides (SUs) are widely used in agriculture owing to their high herbicidal efficacy and low mammalian toxicity. However, their persistence results in residues that pose potential risks to the environment and human health, making it essential to develop rapid and accurate detection techniques for these herbicides. Herein, we designed bionic nanozymes inspired by the structure of acetolactate synthase (ALS), the primary target enzyme of SUs in plants. Using the ALS cofactor thiamine diphosphate and key binding amino acids (arginine, proline, and tryptophan) as ligands, we synthesized bionic nanozymes that exhibited excellent peroxidase-like activity. SUs significantly enhanced the activity of the bionic nanozymes, and the degree of enhancement varied among different SUs. In contrast, organophosphate pesticides (OPs), carbamate pesticides (CPs), and other tested pesticides did not induce such enhancement. Therefore, we constructed a six-channel colorimetric sensor array and achieved 100% accurate discrimination of five SUs across a concentration range of 1–100 µg/mL. Furthermore, the SUs in the four real grain samples and the blind sample were also accurately distinguished by the array. This study not only provides an efficient method for rapid detection of SUs, but also opens new avenues for the bionic design of nanozymes aimed at the precise identification of specific pesticide molecules in complex matrices.
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Analytical Methods
·
2023
·Vol. 15
·期 19
·2376-2381
·
IF 3.1
Lei Jiang,Lin Yuan,Shan Gao,Yingying Xiang,Fei Song,Wensi Ma,Jing Wan,Xiuling Ji,Yujiao Tu
Pesticides are enormously important to industry requirements and in agriculture for improving yields. Parathion is widely used to control pests in vegetables, fruits, and flowers. However, excessive use of parathion poses a threat to food safety, the environment, and human health. Due to low cost, ease to use, and high selectivity and sensitivity, a fluorescent nanoprobe is a candidate for parathion detection. Blue fluorescent carbon dots were prepared by a hydrothermal method with rutinum and o-phenylenediamine as precursors. The Rut-CDs were purified by using dialysis, thin layer chromatography, and a chromatographic column. Excellent linear ranges of 0–7.5 μg L−1 and 12.5–62.5 μg L−1 parathion were obtained with a low detection limit of 0.11 ng mL−1. In addition, the quenching mechanism of parathion to the fluorescence of Rut-CDs was illuminated. Furthermore, the nanoprobe was efficiently applied to determine the parathion content in Chinese cabbage, cantaloupe, and cowpea samples. It shows great potential to detect parathion.
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BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Wanqi Jiang,Zan Yang,Fei Tong,Siyu Zhang,Lu Zhu,Lei Wang,Lunjing Huang,Kang Liu,Mingming Zheng,Yibin Zhou,Ruyan Hou,Yingnan Liu
In this study, based on the oxidase activity and photothermal effect of manganese dioxide nanosheets (MnO 2 NSs), with thiamine (TH) as the fluorescence response signal and tris (2,2'-bipyridyl) ruthenium (II) hexahydrate as the reference signal, an enzyme-regulated ratiometric fluorescence and photothermal dual-mode probe was constructed for the quantitative detection of organophosphorus pesticide (OPs) residues. OPs reduced the production of the reductive product thiocholine by inhibiting the activity of acetylcholinesterase , thereby regulating the residual amount of MnO 2 NSs. With the increase of OPs concentration, the color of the probe solution gradually transitioned from red to blue, and the temperature gradually increased. Using dichlorvos and chlorpyrifos as pesticide models, the developed probes exhibited sensitive responses to OPs in a wide linear range of 0.1–8000 ng/mL. The detection limits of dichlorvos and chlorpyrifos in fluorescence mode were 1.13 × 10 −3 ng/mL and 0.86 ng/mL, respectively. The corresponding detection limits in photothermal mode were 1.01 ng/mL and 1.02 ng/mL, respectively. The proposed probe displayed excellent anti-interference and reliability in the analysis of OPs residues in real samples. The dual-mode probe with self-verification function is expected to provide more accurate and robust detection results than the single-mode probe, and has a wider application prospect.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Yanping Li,Qiong Hu,Jihui Zhang,Hong Zhou,Na Wang,Yishan Fang,Bo Cui
Herein, an enhanced electrochemiluminescence (ECL) aptasensor driven by a complex (T4PPVB-COP@CdS QDs) with large specific surface area and high stability was constructed for highly sensitive detection of chlorpyrifos (CPF), using electrostatic interactions and signal amplification techniques. In the presence of CPF, the specific binding between the aptamer and CPF caused partial detachment of the aptamer from the sensor, thus restoring the ECL signal. Notably, gold nanoparticles functionalized with streptavidin (SA) as signal enhancers further amplified the ECL signal in specific interactions with aptamers, thereby improving the sensitivity of the assay. Based on this, the proposed ECL aptasensor demonstrated significant detection performance for CPF with a linear range of 1–10 7 pg/mL and a LOD of 0.34 pg/mL. Furthermore, the feasibility of the ECL aptasensor was validated by the detection and analysis of CPF in real samples, which also provided a broad reference value for bioanalysis.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Xiaoyu Su,Kaiyi Zheng,Xiaoyu Tian,Xuan Zhou,Xiaobo Zou,Xuechao Xu,Zongbao Sun,Wen Zhang
An indirect and ultrasensitive ratiometric molecularly imprinted (MIP) sensor, based on metal ion reoxidation, is introduced for glyphosate (GLY) determination in fruit. As high-performance signal amplification substrates, carbon nanotubes (MWCNTs) and gold nanoparticles (AuNPs) are conveniently modified on GCE. The artificial antibody-MIP membrane, presents typical three-dimensional structure to GLY template. Built-in reference methylene blue (MB) is directly electropolymerized on MWCNTs-Au/GCE. Particularly, Cu 2+ and GLY interestingly form chelate complex, and the Cu 2+ ( I Cu ) in Cu(Ⅱ)-GLY-complex can be reoxidized, and indirectly quantizes GLY. The reference signal ( I MB ) presents noteworthy stability with different GLY levels, and the ratiometric readout ( I Cu / I MB ) is recognized as a more trustworthy indicator to quantize GLY. Proposed sensor presents broad range as 1.73 ∼ 400 ng/mL, and limit of detection is well found as 0.24 ng/mL (S/N = 3). Finally, as-fabricated method is verified with standard HPLC in real-fruit-sample, and the errors and recovery rates are calculated as 3.4% ∼ 6.7% and 94.4% ∼ 104.6%, respectively.
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MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 9
·1-9
·
IF 6.408
Shang Shuqi,Wang Dongwei,Wang Jiasheng
A dual-mode electrochemical biosensor for acetamiprid detection was proposed for the first time based on carbon quantum dots/Prussian blue (CQDs/PB)-functionalized poly(3,4-ethylenedioxythiphene) (PEDOT) nanocomposite. The nanocomposite with spherical stacking nanostructure showed high surface area, excellent catalytic ability, and cycling stability. The biosensor can be effortlessly constructed after the immobilization of acetamiprid aptamer. The concentration of acetamiprid can be determined by differential pulse voltammetry (DPV) based on its signal change deduced from the pristine PB. With the capture of acetamiprid, the response current (I-T) signal generated by hydrogen peroxide catalysis from the biosensor can also been used to establish the method for monitoring acetamiprid. The dual-mode biosensor showed a wide linear range from 10 −12 g mL −1 to 10 −6 g mL −1 , low detection limits of 6.84 × 10 −13 g mL −1 and 4.99 × 10 −13 g mL −1 , and ultrafast detection time of 25 s and 5 s through DPV and I-T mode, respectively. The biosensor possessed excellent selectivity and stability. More importantly, the biosensor was successfully applied to detect acetamiprid residues in vegetables, proving a promising approach for routine detection of pesticide in real samples. Graphical abstract The biosensor based on PEDOT/CQDs/PB for acetamiprid can be effortlessly constructed through both the increase of differential pulse voltammetry (DPV) signal change deduced by the pristine PB and the decrease of the response current (I-T) signal of the reduction of hydrogen peroxide catalyzed by PEDOT/CQDs/PB.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2022
·Vol. 102
·期 14
·6612-6622
·
IF 4.125
Shaofeng Yuan,Fangwei Yang,Hang Yu,Yunfei Xie,Yahui Guo,Weirong Yao
BACKGROUND Ultrasound has the potential to increase microbial metabolic activity, so this study explored the stimulatory effect of ultrasound pre-treatment on the degradation of four common pesticides (fenitrothion, chlorpyrifos, profenofos, and dimethoate) during milk fermentation by Lactobacillus plantarum and its effect on yogurt quality. RESULTS Appropriate ultrasound pretreatment significantly enhanced the growth of L . plantarum . The degradation percentages of pesticides increased by 19–38% under ultrasound treatment. Ultrasonic intensity, pulse duty cycle, and duration time were key factors affecting microbial growth and pesticide degradation. Under optimal ultrasonic pre-treatment conditions, the degradation rate constants of four pesticides were at least 3.4 times higher than those without sonication. In addition, such ultrasound pretreatment significantly shortened yogurt fermentation time, increased the water holding capacity, hardness and antioxidant activity of the yogurt, and improved the flavor quality of the yogurt. CONCLUSION Ultrasonic pretreatment significantly accelerated the degradation of the four pesticides during yogurt fermentation. In addition, such ultrasound pretreatment increased the efficiency of yogurt making and improved the quality of yogurt in terms of water holding capacity, firmness, antioxidant activity, and flavor. These findings provide a basis for the application of ultrasound to the removal of pesticide residues and quality improvement of yogurt. © 2022 Society of Chemical Industry.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Shuhua Li,Jizhong Wu,Shen Zhang,Tianhui Jiao,Jie Wei,Xiaomei Chen,Qingmin Chen,Quansheng Chen
Herein, we propose a method for detecting thiram based on the fluorescence inner filter effect using upconversion nanoparticles and dithizone–cadmium complexes (DZ-Cd 2+ ). The ultraviolet absorption of DZ-Cd 2+ was in the range of 480–600 nm under alkaline conditions, resulting in fluorescence quenching of the nanoparticles at 540 nm. Thiram had a stronger coordination effect with Cd 2+ than dithizone ; thus, more thiram–cadmium complex (T-Cd 2+ ) formed when thiram was added, leading to fluorescence recovery at 540 nm. The standard thiram curve was found to have a detection limit of 6.75 ng/mL in the linear range of 0.01–1000 µg/mL. In addition, high-performance liquid chromatography results for detecting thiram in apple samples revealed good application performance. The results demonstrate that the developed method has great potential to detect thiram residues in food.
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ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Li Wang,Yi Pan,Zhengwu Wang,Yuanfeng Wang,Xinlin Wei
Organophosphorus pesticides (OPPs) are extensively used in agricultural production, and the contamination caused by their residues has raised significant concerns regarding potential threats to human health. Herein, a novel fluorescence nanoprobe based on an enzyme-mediated silver nanoparticle-modified metal organic framework (AgNPs@PCN-224) was successfully prepared for the rapid detection of OPPs. Initially, AgNPs@PCN-224 were synthesized by reducing silver nitrate (AgNO3) using sodium borohydride (NaBH4) embedded into luminescent PCN-224. This triggered the inner filter effect, leading to fluorescence quenching. Meanwhile, under the catalysis of acetylcholinesterase (AChE) and choline oxidase (CHO), acetylcholine (ATCh) was decomposed to hydrogen peroxide (H2O2), which could destroy AgNPs to form Ag+ released from PCN-224 for fluorescence recovery. Instead, fenitrothion, an OPP, inhibited AChE activity, allowing the quenched fluorescence to be reactivated. Under the current optimum conditions, the fluorescence intensity had a good correlation (Y = −728.5370X + 2178.4248, R2 = 0.9869) over a dynamic range of fenitrothion concentrations from 0.1 to 500 ng/mL, with an LOD of 0.037 ng/mL. In addition, the anti-interference ability and robustness of the proposed sensor was verified for the monitoring of fenitrothion in tea with recoveries of 87.67–103.72% and the relative standard deviations (RSD) < 5.43%, indicating that the system has excellent prospects for OPP determination in practical applications. Furthermore, this work provides a universal platform for screening other enzyme inhibitors to detect OPPs.
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