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PLoS One · 2013 ·Vol. 8 ·期 8 ·e71676 · IF 3.73

Host Deception: Predaceous Fungus, Esteya vermicola, Entices Pine Wood Nematode by Mimicking the Scent of Pine Tree for Nutrient

Feng Lin,Jianling Ye,Huaguang Wang,Aijun Zhang,Boguang Zhao

Background A nematophagous fungus, Esteya vermicola , is recorded as the first endoparasitic fungus of pine wood nematode (PWN), Bursaphelenchus xylophilus , in last century. E. vermicola exhibited high infectivity toward PWN in the laboratory conditions and conidia spraying of this fungus on Japanese red pine, Pinus densiflora , seedlings in the field protected the pine trees from pine wilt disease to some extent, indicating that it is a potential bio-control agent against PWN. Previous research had demonstrated that the living fungal mycelia of E. vermicola continuously produced certain volatile organic compounds (VOCs), which were responsible for the PWN attraction. However, identity of these VOCs remains unknown. Methodology/Principal Findings In this study, we report the identification of α-pinene, β-pinene, and camphor produced by living mycelia of E. vermicola , the same volatile compounds emitted from PWN host pine tree, as the major VOCs for PWN attraction using gas chromatography-mass spectrometry (GC-MS). In addition, we also confirmed the host deception behavior of E. vermicola to PWN by using synthetic VOCs in a straightforward laboratory bioassay. Conclusions/Significance This research result has demonstrated that the endoparasitic nematophagous fungus, E. vermicola , mimics the scent of PWN host pine tree to entice PWN for the nutrient. The identification of the attractive VOCs emitted from the fungus E. vermicola is of significance in better understanding parasitic mechanism of the fungus and the co-evolution in the two organisms and will aid management of the pine wilt disease.

研究领域: 生物学 · 生态学 · 真菌学
✓ 已验证 DOI PubMed 高影响因子
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Polymers · 2020 ·Vol. 12 ·期 1 ·107 · IF 3.426

Modification of Rigid Polyurethane Foams with the Addition of Nano-SiO2 or Lignocellulosic Biomass

Qinqin Zhang,Xiaoqi Lin,Weisheng Chen,Heng Zhang,Dezhi Han

Many achievements have been made on the research of composite polyurethane foams to improve their structure and mechanical properties, and the composite foams have been widely utilized in building insulation and furniture. In this work, rigid polyurethane foams (RPUFs) with the addition of different fillers (nano-SiO2, peanut shell, pine bark) were prepared through the one-step method. The effects of inorganic nano-SiO2and organic biomass on foam properties were evaluated by means of physical and chemical characterization. The characterization results indicate that the compressive strength values of prepared foams could fully meet the specification requirement for the building insulation materials. The inorganic and organic fillers have no effect on the hydrogen bonding states in composite RPUFs. Furthermore, compared to the biomass fillers, the addition of nano-SiO2greatly influenced the final residual content of the fabricated foam. All composite foams exhibit closed-cell structure with smaller cell size in comparison with the parent foam. The prepared composite foams have the potential for utilization in building insulation.Keywords:rigid polyurethane foam;peanut shell;pine bark;nano-SiO2;insulation materials

研究领域: 材料科学 · 化学工程
✓ 已验证 产品关联 DOI PubMed 高影响因子
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CHEMICAL ENGINEERING JOURNAL · 2022 · IF 16.744

Biorhythm paralleled release of pheromone by photothermal conversion for Long-term bark beetle control

Qianhao Pan,Jiaxing Fang,Shiming Zhang,Yonglin He,Yapei Wang

The spruce bark beetle Ips typographus , as the most notorious pest in Eurasia spruce forest ecosystem, mainly feed on phloem of spruce tree trunk. The concealment of their habit makes regulation of insect population a difficulty to cope with, contributing to spruce forest recession, destruction of environment balance and forest transformation from carbon sinks into carbon source. Based on the cross point between the biorhythm of the I. typographus and photothermal material, description herein was a novel sunlight-triggered biorhythm-paralleled photothermal sponge (BPPS) for continuous control of I. typographus . Three steps including preparation of sugar template based porous polyurethane (PU) sponge, polypyrrole (PPy) modification and pheromone filling were involved in material manufacturing. The attractant and repellant agents were employed into the PU separately to realize a comprehensive regulation on I. typographus behavior. Laboratory simulation and forest field experiments illustrated that both the rapid release within a short period and long-term effectiveness in 21 days were verified. Due to the advantages of designability, cost-effectiveness and environmental friendliness of our sustained-release sponge, this manufacturing strategy offers great promise for generating photothermal responsive sustained-release material, shedding light on long-term control of injurious trunk borers and environmental protection.

研究领域: 昆虫学 · 环境科学 · 材料科学
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RSC Advances · 2017 ·Vol. 7 ·期 74 ·46529-46535 · IF 3.108

Hierarchical branched α-MnO2: one-step synthesis and catalytic activity

Hengfa Liu,Bentian Zhang,Wanping Li,Gao Cheng,Jiaxi Han,Bang Lan,Ming Sun,Lin Yu

Hierarchical pine tree-like α-MnO2 architectures are prepared by a facile one-step method without any template. The hierarchical α-MnO2 is composed of branched α-MnO2 short nanorods assembled onto the backbone of an α-MnO2 long nanowire. Time-dependent experiment shows that the growth process of the branched α-MnO2 nanostructures is the formation of β-MnOOH and then the transformation to a α-MnO2 branched structure in a step by step manner. Owing to its specific structure features, the hierarchical pine tree-like α-MnO2 exhibits superior catalytic performance in the catalytic combustion of dimethyl ether with a light-off temperature (T10) at 169 °C and a complete conversion temperature (T90) of 235 °C, which is far better than commercial MnO2.

研究领域: 材料科学 · 催化化学
✓ 已验证 DOI 高影响因子
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INDUSTRIAL CROPS AND PRODUCTS · 2023 · IF 5.9

Fully biodegradable, hydrophobic, enhanced barrier starch bio-composites with sandwich structure by simulating wood

Chuanwei Zhang,Pengfei Zhang,Yanhui Li,S. Nair Sandeep,Jianyong Li,Maocheng Ji,Sixian Peng,Ning Yan,Fangyi Li

Through a single-sided cavity free-form foaming process, composites with open cell structures similar to wood cells were obtained by using corn starch , pulp fiber , and sisal fibers, which was used as the middle layer of the sandwiched composites. Afterwards, more hydrophobic lignin-containing nanocellulose fibrils from pine bark were coated on the surfaces of the open cell composites by high-pressure gun spraying, simulating the bark of wood to impart barrier property to the final product. The water barrier, mechanical property, apparent density, heat insulation, and biodegradability of the sandwich structured bio-composites were characterized. Experimental results indicated that the water contact angle on the surface of the bio-composite was as high as 92°. The apparent density of the bio-composite was very low, at 0.107 g/cm 3 . The tensile strength of the bio-composites reached 5.3 MPa. After conducting biodegradability tests in soil for 60 days, the bio-composites lost 87 % of its mass.

研究领域: 材料科学 · 生物学
✓ 已验证 DOI 高影响因子
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Phytochemistry Letters · 2016 · IF 1.353

Acyl flavone and lignan glucosides from Leontopodium leontopodioides

Yangyang Xiao,Haihui Xie,Li Zhao,Ping Gou

Two new acyl flavone glucosides, luteolin-4⿲- O -(6- p -hydroxybenzoyl)-β- d -glucopyranoside (leontopodioside A, 1 ) and luteolin-4⿲- O -[6-(2-methylbutyryl)]-β- d -glucopyranoside (leontopodioside B, 2 ), and a new acyl lignan glucoside, lariciresinol-9-angeloyl-4- O -β- d -glucopyranoside (leontopodioside   C, 3 ) were isolated from the whole plants of Leontopodium leontopodioides (Asteraceae). Their structures were determined by spectroscopic and chemical methods. Compounds 1 ⿿ 3 exhibited α-glucosidase inhibitory activity with the half maximal inhibitory concentration (IC 50 ) values of 55.6, 39.7, and 474.9   μM, respectively, in comparison with acarbose (626.3   μM).

CAS / MDL: 50-99-7 921-60-8
研究领域: 药用植物学 · 天然产物化学 · 生物化学
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JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS · 2020 · IF 3.905

Comparative chemical analysis of pyrolyzed bio oil using online TGA-FTIR and GC-MS

Mudassir Hussain Tahir,Xingxing Cheng,Rana Muhammad Irfan,Rizwan Ashraf,Yiteng Zhang

The present study proposed successfully TG-FTIR as a novel technique for high quality bio oil production having desirable concentration of targeted products especially phenols and acids. TG-FTIR analysis of pine waste was performed to calculate yield of desirable chemical compounds at different temperature ranged 350−600 °C. While, bio oil was produced at each corresponded temperatures and GC–MS analysis was carried out to support TG-FTIR analysis which showed high consistency. Additionally, efficient extraction of phenolic compounds was performed via complex method using Ca 2+ as a complex agent under alkaline conditions which showed that 95 % phenols were extracted successfully. Meanwhile, production of calcium acetate in residue bio oil turns this study highly valuable for future scientific research.

研究领域: 生物质能 · 分析化学 · 环境科学
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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY · 2017 · IF 2.59

Effects of the plant volatile trans‑2-hexenal on the dispersal ability, nutrient metabolism and enzymatic activities of Bursaphelenchus xylophilus

Yunhe Zhao,Shuangyu Xu,Hongbao Lu,Daxia Zhang,Feng Liu,Jin Lin,Chenggang Zhou,Wei Mu

Bursaphelenchus xylophilus causes pine wilt disease (PWD), which severely damages pine species. The plant volatile trans ‑2-hexenal has strong activity against nematodes, although the precise mechanism of this inhibitory action remains unclear. In this paper, the fumigant effects of the LC 10 and LC 30 of trans ‑2-hexenal on B. xylophilus were demonstrated. The trans ‑2-hexenal treatments significantly inhibited the dispersal ability of nematodes. The results also indicated that trans ‑2-hexenal affects the metabolism of nutrients and the activity of digestive enzymes . Among detoxifying enzymes , after treatment with trans ‑2-hexenal, glutathione S-transferase activity increased significantly and general esterase activity decreased significantly. Based on these results, trans ‑2-hexenal disturbs the normal physiological and biochemical activities of this nematode. These results provide valuable insight into the nematicidal mechanisms of trans ‑2-hexenal.

研究领域: 植物科学 · 线虫学 · 害虫管理
✓ 已验证 产品关联 DOI PubMed 高影响因子
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Sustainable Chemistry and Pharmacy · 2025 · IF 5.8

Green and sustainable ultrasound–microwave hybrid system for intensified and efficient quercetin extraction from Pinus koraiensis seed scales

Zhanjun Li,Yunwei Liu,Mingru Kong,Zheng Qin,Qi Shen,Mengze Wang

The primary objective of this study was to achieve high-efficiency, sustainable method for extracting quercetin from Pinus koraiensi s seed scales using a green chitosan–Cu–Fe catalyzed ultrasound–microwave continuous system. Key factors were screened and optimized, with the following optimal conditions: a liquid material ratio of 10:1 mL/g for both ultrasonic and microwave treatments, ultrasonic 10 min, 90 W, 40 °C; microwave irradiation at 303 W for 8.19 min; and exogenous chitosan–Cu–Fe at 0.6 %, achieving an actual yield of 3.13 mg/g. The optimal process was compared with conventional Soxhlet extraction, used as a benchmark to evaluate the new method's efficiency. Kinetic analysis showed equilibrium at 12.5 and 50 min, with yields of 3.33 and 3.23 mg/g, respectively, while prolonged high-temperature exposure extraction caused partial degradation of the extract. Life cycle assessment and inductively coupled plasma mass spectrometry confirmed the superior stability and environmental sustainability of the optimal process. Mechanistic evaluation indicated that the extraction followed a second-order kinetics model; with the second-stage activation energy (87.56 kJ/mol) primarily governed by extraction, indicating that regulating the temperature enhances efficiency and reduces losses. Ultrasonic cavitation and microwave heating enhance extraction efficiency, while chitosan–Cu–Fe improves stability by increasing active sites and facilitating electron transfer. The process offers environmental and sustainability benefits, providing insights into the valorization of forest residues.

CAS / MDL: 111-30-8 9012-76-4
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CHEMICAL ENGINEERING JOURNAL · 2025 · IF 13.2

Conduction of AgNPs with different surface charges in pine trees and their prevention and control of pine wood nematode disease

Shouhe Pan,Pengkun Yan,Rui Zhao,Fengyu Li,Lei Wang,Yinmin Wang,Ze Lv,Yingjian Ma,Meng Yu,Xinyu Guo,Yonggang Xia,Yong Xu,Xuemin Wu

Pine wood nematode disease is caused by pine wood nematodes and is difficult to prevent and control because the nematodes are located inside the trees. In this study, three types of silver nanoparticles (AgNPs) with different surface charges were prepared using Cetyl trimethyl ammonium chloride (CTAC), Polyvinyl pyrrolidone (PVP), and Acacia senegal (GA), and the ion and element concentrations in tree sap were measured after the introduction of AgNPs. Additionally, the effects of the introduction of AgNPs by foliar and soil applications and by trunk injection on the delivery of the AgNPs in the trees were compared. It was found that the LC 50 values of AgNP-GA, AgNP-PVP, and AgNP-CTAC against pine wood nematodes were 0.59, 1.15, and 1.25 mg∙L − 1 , respectively. Our toxicity mechanism studies showed that AgNP can inhibit the activity of acetylcholinesterase and catalase in pine wood nematodes, which is the main origin of the inhibition of pine wood nematode activity by AgNPs. The ion concentration of the sap has a significant impact on the delivery of AgNPs within the tree, with negatively charged AgNP-GA displaying better delivery within pine trees. In addition to conduits and sieve tubes, resin channels and intercellular spaces are likely to be the key sites for AgNP delivery. It was found that trunk injection facilitates the delivery of AgNPs within trees. This study provides an effective strategy for the prevention and control of pine wilt disease, tree trunk pests, and other diseases.

研究领域: 植物病理学 · 纳米技术 · 林业科学 · 农药科学 · 树木生理学 · 生物化学
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CARBOHYDRATE POLYMERS · 2020 · IF 7.182

Inhibition effect of three common proanthocyanidins from grape seeds, peanut skins and pine barks on maize starch retrogradation

Mengting Wang,Jianchu Chen,Shiguo Chen,Xingqian Ye,Donghong Liu

The inhibition effect of three common proanthocyanidins (PA) on the retrogradation properties of maize starch was investigated (including grape seed proanthocyanidins (GSPA), peanut skin proanthocyanidins (PSPA), and pine bark proanthocyanidins (PBPA)). Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) analysis showed that PA could significantly decrease the values of melting enthalpy of retrogradation ( ΔH r ) and the degree of relative crystallinity, suggesting that the starch re-crystallization was retarded by PA. Scanning electron microscope (SEM) characterizations illustrated that retrograded PA-starch samples formed a looser matrix with less appearance of continuous flakes during storage. Overall, 0.5 %–2.0 % of three PAs exhibited suppression of starch retrogradation after 21-day cooling storage, mainly resulting from the PA-starch interaction. Among them, PSPA showed the most substantial inhibition effect on starch retrogradation, which might be attributed to its structural features. This study suggested that PA could be a new type of inhibitor to suppress starch retrogradation.

CAS / MDL: 9005-25-8
研究领域: 食品科学 · 化学 · 农业科学
✓ 已验证 产品关联 DOI PubMed 高影响因子
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Frontiers in Microbiology · 2025 · IF 4.5

Synergistic effects of beneficial microbial inoculants and SMS-amendments on improving soil properties and Pinus seedling growth in degraded soils

Xuan Bo,Yong Liu,Hong Zhang,Chao Su,Yang Miao

Spent mushroom substrate (SMS) is a promising organic amendment for remediating degraded soils, yet its effectiveness is often limited by poor compost maturity and stability. This study aimed to enhance the quality and functionality of SMS compost through microbial inoculation and to evaluate its impact on soil improvement and plant growth. Three plant growth-promoting strains [Bacillus subtilis (B. subtilis), Azotobacter chroococcum (A. chroococcum), and Paenibacillus mucilaginosus (P. mucilaginosus)] and their combination as a mixed microbial inoculant (MMI) were used in composting. Subsequent pot experiments assessed changes in soil physicochemical properties, nutrient levels, microbial diversity, and the growth of Pinus sylvestris seedlings. Results showed that SMS amended with MMI significantly improved soil porosity, pH, and nutrient content, while enriching beneficial microbial communities dominated by Proteobacteria and Basidiomycota. Moreover, MMI treatment notably enhanced plant height, stem diameter, and chlorophyll content compared to control treatments. These findings highlight the synergistic effect of mixed microbial inoculants in optimizing SMS compost and promoting soil and plant health. The approach offers a sustainable strategy for the circular reuse of agricultural waste and effective restoration of degraded soils.

研究领域: 土壤科学 · 微生物学 · 植物学 · 农业可持续性 · 环境修复
✓ 已验证 DOI PubMed 高影响因子
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