RSC Advances
·
2019
·Vol. 9
·期 48
·28248-28252
·
IF 3.049
Yumin Leng,Fang Hu,Chunhua Ma,Chenxi Du,Linfeng Ma,Jiao Xu,Qiyuan Lin,Zhipei Sang,Zhiwen Lu
A simple, rapid, sensitive, selective and label-free method is presented for the colorimetric determination of lincomycin (Lin) by using HAuCl4 and NaOH. Upon the addition of Lin, the mixture of HAuCl4 and NaOH shows a color change from colorless to blue (or dark blue). The limit of colorimetric detection is as low as 1 μM, observed both in Milli-Q water and real samples. The selectivity of Lin detection is excellent compared with 9 other common antibiotics. On the basis of the “three-color” principle of Thomas Young, we extracted the red, green and blue (RGB) alterations of the sensor in the absence and presence of different concentrations of Lin. The color changes are quantitatively illustrated by the total Euclidean distances (EDs = [ΔR2 + ΔG2 + ΔB2]1/2). The good linear relationship between the EDs and Lin concentration is used for the quantitative assay of Lin. The developed method demonstrates great potential for the detection of Lin in environmental water and milk.
研究领域:
分析化学
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环境科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2019
·
IF 4.259
Kang Yajing,Wang Yingying,Hou Bingbing,Wang Ruida,Ye Jiang,Zhu Xiaoyu,Wu Haizhen,Zhang Huizhan
Lincomycin is one of the most important antibiotics in clinical practice. To further understand the regulatory mechanism on lincomycin biosynthesis, we investigated a pleiotropic transcriptional regulator AdpAlin in the lincomycin producer Streptomyces lincolnensis NRRL 2936. Deletion of adpAlin (which generated ΔadpAlin) interrupted lincomycin biosynthesis and impaired the morphological differentiation. We also found that putative AdpA binding sites were unusually scattered in the promoters of all the 8 putative operons in the lincomycin biosynthetic gene cluster (BGC). In ΔadpAlin, transcript levels of structural genes in 8 putative operons were decreased with varying degrees, and electrophoretic mobility shift assays (EMSAs) confirmed that AdpAlin activated the overall putative operons via directly binding to their promoter regions. Thus, we speculated that the entire lincomycin biosynthesis is under the control of AdpAlin. Besides, AdpAlin participated in lincomycin biosynthesis by binding to the promoter of lmbU which encoded a cluster sited regulator (CSR) LmbU of lincomycin biosynthesis. Results of qRT-PCR and catechol dioxygenase activity assay showed that AdpAlin activated the transcription of lmbU. In addition, AdpAlin activated the transcription of the bldA by binding to its promoter, suggesting that AdpAlin indirectly participated in lincomycin biosynthesis and morphological differentiation. Uncommon but understandable, AdpAlin auto-activated its own transcription via binding to its own promoter region. In conclusion, we provided a molecular mechanism around the effect of AdpAlin on lincomycin biosynthesis in S. lincolnensis, and revealed a cascade regulation of lincomycin biosynthesis by AdpAlin, LmbU, and BldA.
研究领域:
微生物学
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分子生物学
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抗生素研究
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产品关联
DOI
PubMed
高影响因子
Journal of Cleaner Production
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2019
·
IF 6.395
Qiong Ouyang,Fangying Kou,Pokeung Eric Tsang,Jintao Lian,Jingyi Xian,Jianzhang Fang,Zhanqiang Fang
Iron-based catalysts (GFe0.5) were successfully prepared from tea polyphenols (TP) and ferric trichloride and used as a heterogeneous Fenton-like catalyst for the degradation of lincomycin (LCM). The results showed that a GFe0.5 dosage of 0.01 g/L could completely degrade a 20 mg/L concentration of LCM in 90 min, and assessment of the toxicity to luminous bacteria showed that the GFe0.5/H 2 O 2 system effectively reduced the toxicity risk posed by this pollutant. The results of transmission electron microscopy , X-ray diffraction (XRD), Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and other characterisations showed that the synthetic material had a triangular structure similar to that of nonacarbonyldiiron, with Fe 2+ , Fe 3+ and organic groups attached to the triangular structure's surface. That is, the present environment-friendly synthesis produces an Fe-based material resembling a mixture of nonacarbonyldiiron and ferrous chloride. Free-radical capture and electron spin resonance experiments confirmed that ·OH was the main active free radical in the degradation mechanism of the iron-based material. Cyclic experiments, inductively coupled plasma and XRD characterisation of the materials after the reaction showed that the loss of free iron was the main reason for the decrease of activity. The free iron in the Fe-based material becomes bound during the reaction, which effectively avoids the excessive consumption of Fe 2+ in the prophase of the Fenton-like reaction, and is the key to both the efficient Fenton-like catalytic ability of GFe0.5, and to preventing the production of iron sludge .
研究领域:
环境科学
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材料科学
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DOI
高影响因子
OPTICS AND SPECTROSCOPY
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2015
·Vol. 118
·期 5
·748-755
·
IF 0.723
Ge Baoyu,Li Zhigang,Xie Yuanzhe,Yang Lingling,Wang Ruiyong
Two novel and convenient methods for the determination of lincomycin (LCM) in aqueous solutions have been developed. The first method was based on the enhanced fluorescence of thioglycolic acidcapped CdTe quantum dots (TGA-CdTe QDs) by LCM. For the second method, the introduction of LCM could induce the aggregation of gold nanoparticles (AuNPs), displaying distinct changes in color and in UVvis spectra. Under optimal conditions, the enhanced fluorescence intensity was linearly proportional to LCM concentration in the range of 1–240 μg mL −1 with a detection limit of 2.63 × 10 −1 μg mL −1 . The second platform is capable of determining LCM in ranges from 1.00 × 10 −3 to 2.00 × 10 −2 μg mL −1 and from 3.00 × 10 −2 to 1.20 × 10 −1 μg mL −1 with a detection limit of 1.27 × 10 −4 μg mL −1 . Both methods were used for rapid detection of LCM in real samples with satisfactory results. Comparisons between the two methods were made.
研究领域:
分析化学
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纳米生物学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Zeqing Dong,Jian Lu,Yilin Wu,Minjia Meng,Chao Yu,Chang Sun,Muning Chen,Zulin Da,Yongsheng Yan
As a veterinary antibiotic, lincomycin (LIN) residues in milk are raising concerns of public on account of potential harm to human health. Efficient strategy is eagerly desired for detection of LIN from milk samples. Hence, lincomycin molecularly imprinted membranes (LINMIMs) were developed for selective separation of LIN as an efficient pretreatment of milk samples. The synergistic effect of polyethylenimine and dopamine provided effective antifouling performance by improving the hydrophilicity. Based on click chemistry, specific recognition sites were facilely formed on membranes using 4-vinylpyridine as functional monomers. The satisfactory rebinding capacity (151.62 mg g −1 ), permselectivity (4.43), together with the linear dependence ( R 2 = 0.9902) of concentrations in eluents and original samples. Moreover, the method was utilized to determine LIN from milk, with good recovery and relative standard deviation. Achievements in this work will actively promote the development of efficient detection technology.
研究领域:
食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2024
·Vol. 149
·期 6
·1746-1752
·
IF 3.6
Xiaohui Chen,Jing Wen,Xueling Shan,Wenchang Wang,Zhidong Chen
In this work, the supersensitive and selective determination of lincomycin (Lin) was achieved using a novel electroluminescent (ECL) aptasensor based on the synergistic integration of gold functionalized upconversion nanoparticles (UCNPs) and thiolated 3,4,9,10-perylene tetracarboxylic acid (PTCA). The integration of two luminophores of UCNPs and PTCA combined the merits of the cathodoluminescence stability of UCNPs and the high quantum yield of PTCA, which significantly promoted the ECL signal and analytical performance of the proposed sensor. The introduction of gold nanoparticles in UCNPs can not only improve the conductivity and ECL performance of UCNPs but also cause them to easily integrate with thiolated PTCA (t-PTCA) via an Au-S bond. The ECL signal of UCNPs@Au/t-PTCA/GCE was almost twice as strong as that of t-PTCA/GCE and tenfold higher than that of UCNPs@Au/GCE. Because of the non-conductive protein of the Lin aptamer, the ECL intensity of apt/UCNPs@Au/t-PTCA/GCE noticeably decreased. In the presence of Lin, the aptamer was pulled down from the sensing interface, resulting in the recovery of the ECL intensity of the sensor. Under optimal conditions, our proposed sensor can quantify the concentration of Lin in the range from 1.0 × 10−15 to 1.0 × 10−7 M with a low detection limit of 2.4 × 10−16 M (S/N = 3), exhibiting high sensitivity and specificity for the determination of Lin.
研究领域:
分析化学
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生物传感
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药物检测
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2019
·
IF 8.355
Qiong Ouyang,Jintao Lian,Baizhou Lu,Zhanqiang Fang
In this paper, the effects of lincomycin (LCM) degradation by six typical promoters such as tea polyphenols (TP), oxalic acid (OA), ethylenediaminetetraacetic acid disodium (EDTA), hydroxylamine hydrochloride (HA), sodium tripolyphosphate (TPP) and ascorbic acid (AA) on the Fe 0 /H 2 O 2 system based on microscale zero valent iron (mZVI) were compared through batches of experiments. The degradation effects of promoters on the Fe 0 /H 2 O 2 system in 90 min showed significant promotion (OA, AA and HA), moderate promotion (TP) and inhibition (EDTA, TPP), respectively. Moreover, this result was very consistent with the results of •OH and ORP. First, by studying the effects of each promoter on the pH value and the iron leaching of the Fe 0 /H 2 O 2 system, it was preliminarily proved that the main reason for the enhancement of Fenton-like degradation of OA, HA and AA is to lower the pH and thus promote iron leaching. Then, through comparative experiments of reducing and chelating abilities of six promoters, it was proved that TP promoted the Fenton-like degradation mainly by reduction and chelation without significantly changing the pH of the system. Although EDTA and TPP have strong chelating ability and ability to promote iron leaching, respectively, they caused the pH to be too high, which inhibited the reaction. The results show that lowering the pH of system, promoting iron leaching, reducing ferric iron and chelating iron ions to avoid the formation of iron sludge are the main mechanisms of the current Fenton-like promoters. Moreover, the combination of multiple promotion methods can better adapt to different reaction conditions.
研究领域:
环境科学
·
化学工程
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Qiong Ouyang,Fangying Kou,Nuanqin Zhang,Jintao Lian,Guoquan Tu,Zhanqiang Fang
Tea polyphenols (TP) are natural green organic compounds with chelating and reducing properties, which can promote the catalytic reaction, but the mechanism of promotion is still uncertain. In this paper, the chelation and reduction of TP were used to promote the degradation of lincomycin (LCM) by zero-valent iron Fenton-like reaction, and the mechanism of the two properties of TP was analyzed. The results showed that in the Fe 0 /H 2 O 2 system, the degradation rate of the reaction system was increased from 27% to 100% in two hours after the addition of TP, and the reaction rate was increased about 26 times. Moreover, the parameters of pH, Fe 2+ , ·OH, oxidation reduction potential (ORP) and so forth indicating that the chelating structure of TP and iron ions was formed under pH = 4–9, which obviously promoted the mutual conversion of Fe 3+ /Fe 2+ . It is propitious to the proper amount of iron dissolution in order to facilitate the continuous and efficient Fenton reaction. Under acidic conditions, the TP are more reductive due to the destruction of the chelate structure, which promotes the conversion of Fe 3+ to Fe 2+ or even zero-valent iron, significantly improves the Fe 3+ /Fe 2+ cycle capacity and increases the amount of OH produced.
研究领域:
环境科学
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化学
·
生物学
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产品关联
DOI
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 35
·4501-4510
·
IF 2.378
Pu Wu,Qiuzheng Du,Yiyang Sun,Zhonghong Li,Hua He
Lincomycin (LCM) has low ultraviolet (UV) absorption and thus, its determination requires complex instrumentation. Herein, a novel fluorometric probe based on an europium metal–organic framework coated with molecularly imprinted polymers (Eu(BTC)-MIP) was fabricated for the selective determination of LCM. First, highly luminescent Eu(BTC) was synthesized at room temperature. Then, through a simple co-polymerization process, MIPs were formed on the Eu(BTC) surface in the presence of LCM as template molecules to obtain Eu(BTC)-MIP. Eu(BTC) acted as a supporting substrate and antenna, and the MIP layer provided specific binding sites for LCM. Due to the advantages of both Eu(BTC) and MIPs, the probe had a long emission lifetime, strong fluorescence and specific recognition. Under the optimized conditions, the probe achieved the selective determination of LCM in the concentration range of 10 to 100 μg L−1 with a detection limit (LOD) of 7.18 μg L−1 (S/N = 3). This method is attractive because of its low cost, good selectivity, ready availability and simple manipulation without the need for complex instrumentation. Furthermore, the probe was successfully employed to detect LCM in egg samples with a recovery in the range of 88.02% to 97.29% and a relative standard deviation (RSD) of less than 3.46%. The satisfactory results demonstrate that the proposed probe based on Eu(BTC)-MIP can be used for the quantitative analysis of LCM and exhibits great potential for LCM determination in food samples. Also this study provides a new perspective for the determination of weak ultraviolet absorption or non-fluorescent substances without conjugated groups.
研究领域:
分析化学
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食品科学
·
材料科学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Xiaojiao Du,Jun Sun,Yan Li,Wenhan Du,Ding Jiang
A highly sensitive electrochemiluminescence (ECL) aptasensor for lincomycin (Lin) detection was designed based on a novel self-accelerated ECL luminophore of Ag 3 PO 4 -Ti 3 C 2 nanohybrids. The Ag 3 PO 4 -Ti 3 C 2 nanohybrids demonstrated prominent ECL performances with K 2 S 2 O 8 as co-reactant, and the Ti 3 C 2 as co-reaction accelerator, which catalyzes K 2 S 2 O 8 into more active radicals and inducing a tremendous amount of excited-state luminophores. Besides, it can be used for loading aptamer molecules owing to its large surface area. Given these attributes, a self-accelerated ECL aptasensor for Lin detection was constructed based on the effect of target induced aptamer dissociating. Under optimized conditions, the ECL aptasensor exhibited wide linear relationship of 1.0 × 10 −4 nmol/L to 1.0 × 10 2 nmol/L, low detection limit of 6.17 × 10 −5 nmol/L (S/N = 3) and good stability and reproducibility for Lin detection. The aptasensor was successfully applied for the Lin detection in milk and water samples, implying immense potential in real applications.
研究领域:
分析化学
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生物传感器
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纳米技术
·
药物分析
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2022
·
IF 14.224
Huaxin Lei,Jiayu Zhang,Jin Huang,Dengjin Shen,Yin Li,Rui Jiao,Renxin Zhao,Xiaoyan Li,Lin Lin,Bing Li
The aerobic, lincomycin-degrading bacterial strain Conexibacter sp . LD01, belonging to the phylum Actinobacteria, was isolated from activated sludge . Both second- and third-generation sequencing technologies were applied to uncover the genomic characterization and high-quality genome with 99.2% completeness and 2.2% contamination was obtained. The biodegradation kinetics of lincomycin fit well with the modified Gompertz model ( R 2 > 0.97). Conexibacter sp . LD01 could subsist with lincomycin as the sole source of carbon, nitrogen, and energy. When 500 mg/L of glucose was added as a co-substrate, the biodegradation rate improved significantly, whereas the addition of 500 mg/L sodium pyruvate had a slight inhibitory effect. Ammonia nitrogen was the best nitrogen source for Conexibacter sp . LD01 when growing and degrading lincomycin. In total, 17 metabolic products consisting of nine novel products were detected, and five biodegradation pathways, including N-demethylation, breakage of the amido bond, sulfoxidation, and oxidation of the pyrrolidine ring and propylamino chain, were proposed. This study significantly expands our understanding of the functional microorganisms and mechanism involved in lincomycin biodegradation at the phylum level.
研究领域:
微生物学
·
环境科学
·
基因组学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2024
·
IF 4.1
Yahui Zhang,Jing Wang,Yongping Dong
In the present work, the nanosheets of iridium complex (Ir NS) were synthesized by surfactant assisted reprecipitation method. Strong anodic ECL signal of Ir NS was obtained under neutral aqueous condition with electrogenerated hydroxyl radical as coreactant, without the need of additional coreactant. Excellent light absorption ability of gold nanoparticles decorated Cu 2 O cubic nanoparticles (Cu 2 O@Au) could apparently inhibit the ECL intensity of Ir NS. When Cu 2 O@Au was assembled onto the electrode surface by base pair complementarity of DNA, the ECL resonance energy transfer (ECL-RET) system could be fabricated with Ir NS ECL as energy donor and Cu 2 O@Au as energy acceptor. The specific interaction of lincomycin (LIN) with the aptamer could release Cu 2 O@Au from the electrode surface, resulting in the recovery of ECL signal. Based on this, an “off-on” mode ECL sensor was designed to detect LIN. Under the optimal condition, the ECL intensity changed linearly with the logarithm of LIN concentration in the range of 1.0 × 10 −11 –1.0 × 10 −6 mol L −1 with a detection limit of 2.88 × 10 −13 mol L −1 . Importantly, the proposed ECL sensor showed outstanding accuracy and specificity in the detection of LIN, and provided a potential strategy for the determination of antibiotic.
研究领域:
分析化学
·
生物传感器
·
纳米技术
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）