FIBERS AND POLYMERS
·
2024
·Vol. 25
·期 7
·2669-2682
·
IF 2.2
Luo Laipeng,Ding Chenkun,Zhu Qinting,Liu Lei,Wang Ping,Wang Qiang,Yu Yuanyuan
Untreated natural cotton fabrics are hydrophobic due to the high content of pectin and waxes, and the fabrics need to improve the wettability by scouring for the demands of the subsequent processing such as dyeing and finishing. In this study, a new scouring method for cotton fabrics was developed by pectin hydrolase combined with Fenton system (Fe 2+ /H 2 O 2 ). The mechanism of pectin degradation by pectinase combined with Fe 2+ /H 2 O 2 was investigated. The results showed that α-1,4-glycosidic bonds of water-soluble pectin could be degraded more efficiently by pectinase hydrolysis and Fe 2+ /H 2 O 2 oxidation. The fabrics scoured with pectinase → Fe 2+ /H 2 O 2 had less wetting time (4.25 s) and higher vertical wicking height (9.80 cm) compared to the fabrics treated with pectinase alone (> 120 s, 5.15 cm). The results of bleaching and dyeing experiments indicated that the whiteness and dyeing properties of the fabrics were not negatively affected by pectinase → Fe 2+ /H 2 O 2 treatment, and the strength loss of the fabrics (11.79%) was lower than that of the fabrics with conventional NaOH treatment (20.16%). The results of the experiments in the pilot-scale machine showed that various performance indicators of the fabrics scoured by pectinase → Fe 2+ /H 2 O 2 reached the level of the conventional NaOH treatment, which demonstrated the possibility for practical application of pectinase → Fe 2+ /H 2 O 2 scouring. The cotton fabric scouring using pectinase → Fe 2+ /H 2 O 2 is an effective and feasible new method with advantages such as environmental friendliness, mild treatment conditions, and good treatment effect.
研究领域:
纺织科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Nanomaterials
·
2024
·Vol. 14
·期 24
·2022
·
IF 4.4
Qiongmei Mai,Yu Lu,Qianyu Cai,Jianglong Hu,Yunyou Lv,Yonglan Yang,Liqiang Wang,Yuezhao Zhou,Jie Liu
Sclerotinia sclerotiorumis one of the fungi that cause plant diseases. It damages plants by secreting large amounts of oxalic acid and cell wall-degrading enzymes. To meet this challenge, we designed a new pH/enzyme dual-responsive nanopesticide Pro@ZnO@Pectin (PZP). This nanopesticide uses zinc oxide (ZnO) as a carrier of prochloraz (Pro) and is encapsulated with pectin. When encountering oxalic acid released bySclerotinia sclerotiorum, the acidic environment promotes the decomposition of ZnO; at the same time, the pectinase produced bySclerotinia sclerotiorumcan also decompose the outer pectin layer of PZP, thereby promoting the effective release of the active ingredient. Experimental data showed that PZP was able to achieve an efficient release rate of 57.25% and 68.46% when pectinase was added or under acidic conditions, respectively. In addition, in vitro tests showed that the antifungal effect of PZP was comparable to that of the commercial Pro (Pro SC) on the market, and its efficacy was 1.40 times and 1.32 times that of the Pro original drug (Pro TC), respectively. Crucially, the application of PZP significantly alleviated the detrimental impacts of Pro on wheat development. Soil wetting experiments have proved that PZP primarily remained in the soil, thereby decreasing its likelihood of contaminating water sources and reducing potential risks to non-target organisms. Moreover, PZP improved the foliar wettability of Pro, lowering the contact angle to 75.06°. Residue analyses indicated that PZP did not elevate prochloraz residue levels in tomato fruits compared to conventional applications, indicating that the nanopesticide formulation does not lead to excessive pesticide buildup. In summary, the nanopesticide PZP shows great promise for effectively managingSclerotinia sclerotiorumwhile minimizing environmental impact.
研究领域:
植物病理学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CELLULOSE
·
2021
·Vol. 28
·期 13
·8375-8386
·
IF 5.044
Fan Fangwei,Zhu Mengting,Fang Kaiyang,Xie Jinpeng,Deng Zhongmin,Wang Xianfeng,Zhang Zhen,Cao Xinwang
This paper reports an improved traditional fiber degumming method, where sisal fibers were treated by alkali oxygen and pectinase, respectively, after the solute alkali pretreatment. To explore the influence of various factors on its degumming, efficiency of degumming through single factor and orthogonal experiments was aasessed. The results showed that pectinase/alkali-oxygen method after the first alkali treatment had a good effect on the degumming of sisal fiber, and most of the non-cellulose components such as hemicellulose, lignin and pectin had been removed. After pectinase treatment, the cellulose content and crystallinity were 71.87% and 66.29%, respectively. After alkaline oxygen treatment, the cellulose content was 77.16%, and the crystallinity was 69.09%. In terms of degumming rate, alkali oxygen treatment worked better than pectinase treatment, the degumming rate of pectinase method was about 10%, while that of alkali-oxygen method was more than 20%. In other hand, the pectinase method was much milder and had less damage to fibers. It would provide some references for the future application and development of sisal fiber.
研究领域:
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2018
·
IF 5.089
Ziyu Deng,Fang Wang,Bin Zhou,Jing Li,Bin Li,Hongshan Liang
In this study, polygalacturonase (PG) was immobilized in calcium alginate microspheres prepared using endogenous emulsification method and its application in apple juice clarification was evaluated. Preparation conditions of calcium alginate microspheres prepared by endogenous emulsification were optimized, additionally, the formed microspheres were characterized. Under optimal conditions, microspheres with the particle size of 38.0 ± 0.2 μm showed good sphericity, storage stability and a narrow particle size distribution. Meanwhile, single factor experiment and response surface experiment were combined to get the optimum conditions of immobilizing PG. The immobilized enzyme presented the optimum temperature at 60.0 °C, which was almost 10.0 °C higher than the free PG and the activity was less dependent upon pH within the interval of 3.0–4.5 than free enzyme, supporting the immobilized enzyme more stable in apple juice clarification (pH = 4.3 ± 0.1). The immobilized enzyme also exhibited good operational stability and 63.0% of its initial activity retained after three batch reactions. In the present of the immobilized PG, the turbidity of apple juice decreased, as well as apple juice had good thermal and freezing ability.
研究领域:
食品科学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yinchun Liu,Fang Liu,Jianrui Liu,Jingwen Dong,Mingxia Xing,Xiangyu Chen,Ya'nan Wu,Tianxi Ai,Yongqin Zhang
Pectin depolymerase is widely utilized in various industrial sectors. However, the traditional methods for determining its enzymatic activity have limitations, such as cumbersome operations and a significant impact of enzyme solution dilution ratios on activity. The 3-methyl-2-benzothiazolinone hydrazone (MBTH) method can be employed to address these issues, but pectin precipitation and strong background commonly arise in this method. We have successfully overcome these challenges by employing a low-temperature and high-alkaline environment, and further optimized the reagent compositions and detection wavelength to improve the method. Consequently, enzyme hydrolysis follows a zero-order reaction within 60 min, which is helpful for the endpoint measurement of pectinase activity. The developed calibration curve for pectinase concentration and hydrolysis rate demonstrates linearity (R 2 = 0.9945) within the range of 2.5–15.8 mU/mL of pectinase. This method exhibits high sensitivity, accuracy, and stability, making it suitable for routine determination of pectin depolymerase activity in research and applications.
研究领域:
生物化学
·
工业酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Bioresources and Bioprocessing
·
2017
·Vol. 4
·期 1
·1-8
·
IF 0
Sun Yang,Ding Song,Huang He,Hu Yi
Background In recent years, ionic liquids and enzymes have been widely used in the separation and extraction processes of natural products. Chlorogenic acid (CGA) has important biological and pharmacological activities. It is significant to develop a green and efficient method to extract GCA from Flos Lonicera japonica (FLJ) by integrating the advantages of the ionic liquids and enzymes. Results The optimal type of enzyme and ionic liquid was screened. Pectinase in [C6mim] Br aqueous phase was demonstrated to be an ideal combination. The parameters including extraction time, extraction temperature, pH, enzyme amount, and IL concentration were optimized systematically. Scanning electronic microscopy of FLJ samples demonstrated that pectinase and ionic liquid disposal both obviously facilitated the extraction process by destroying the structure of cell wall. Circular dichroism spectroscopy showed that ionic liquid enhanced the activity of the pectinase by altering its secondary structure. Conclusions Compared with previous reported methods, ionic liquid-based enzyme-assisted extraction of GCA from FLJ was proved to be efficient and practical, offering a higher yield in a shorter time. A novel process was proposed for the extraction of active component from natural resources.
研究领域:
植物化学
·
天然产物提取
·
生物技术
✓ 已验证
DOI
PubMed
FOOD CHEMISTRY
·
2018
·
IF 4.946
Xiao-Yan Dai,Li-Min Kong,Xiao-Ling Wang,Qing Zhu,Kai Chen,Tao Zhou
Pectinase was immobilized onto sodium alginate/graphene oxide beads via amide bonds by using N , N ′-dicyclohexylcarbodiimide/ N -hydroxysuccinimide as the activating agent. The immobilized pectinase was characterized by Fourier transform infrared spectra and scanning electron microscopy analyses. Immobilization conditions were optimized by Box–Behnken design and the response surface method. The activity of the immobilized pectinase prepared under optimal conditions reached 1236.86 ± 40.21 U/g, with an enzyme activity recovery of 83.5%. The optimal pH of free pectinase was 4.5, while that of immobilized pectinase was shifted to 4.0. The optimal temperature of immobilized pectinase was increased to 60 °C, which was 10 °C higher than that of free form. Furthermore, the immobilized pectinase possessed a superior thermal stability and storage stability to those of free pectinase. Reusability studies indicated that the immobilized pectinase retained 73% of initial activity after six times cycles. Due to these good properties, such immobilized pectinase may find application in food industry.
研究领域:
生物化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 5
·1035
·
IF 4.2
Kaihua Guo
Enzymatic hydrolysis using pectinase is critical for producing high-yield and quality sea buckthorn juice. This study determined the optimal temperature, time, and enzyme dosage combinations to guide manufacturers. A temperature of 60 °C, hydrolysis time of 3 h, and 0.3% enzyme dosage gave 64.1% juice yield—25% higher than without enzymes. Furthermore, monitoring physicochemical properties reveals enzyme impacts on composition. Higher dosages increase soluble solids up to 15% and soluble fiber content by 35% through cell wall breakdown. However, excessive amounts over 0.3% decrease yields. Pectin concentration also declines dose-dependently, falling by 91% at 0.4%, improving juice stability but needing modulation to retain viscosity. Electrochemical fingerprinting successfully differentiates process conditions, offering a rapid quality control tool. Its potential for commercial inline use during enzymatic treatment requires exploration. Overall, connecting optimized parameters to measured effects provides actionable insights for manufacturers to boost yields, determine enzyme impacts on nutrition/functionality, and introduce novel process analytical technology. Further investigations of health properties using these conditions could expand sea buckthorn juice functionality.
研究领域:
食品科学
·
酶工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2025
·
IF 6.2
Jie Hao,Xusong Wang,Liqun Li,Xuhui Huang,Na Wu,Tao Yu,Lu An,Lei Qin
The effect of exogenous enzyme treatment on hazardous substances and small-molecule compounds in smoke was investigated by treating Yunyan 87 tobacco with mesophilicα-amylase, pectinase, and lipase. The three enzymes could effectively diminish the total particulate matter (TPM) and nicotine-free dry particulate matter (NFDPM) content in smoke. Notably, mesophilicα-amylase demonstrated the most significant reduction, achieving a decrease of 8.15 % in TPM and 7.08 % in NFDPM content. The efficacy of these three enzymes in reducing phenol levels was remarkable, with a reduction exceeding 20 %. Pectinase and lipase exhibited superior performance in diminishing hydrogen cyanide (HCN) content, achieving a reduction of 13 %. The relative abundance of aroma-related and alkaloids compounds in smoke exhibited varying alterations after different enzyme treatments. The utilization of mesophilicα-amylase exhibited a favorable influence on the augmentation of aroma-related compounds, such as β-damascone and α-cyperone, whereas the effect of pectinase was opposite. The application of pectinase played a crucial role in the reduction of tobacco alkaloids and their related compounds. The occurrence of defects is unavoidable during the growth and curing process of tobacco leaves. Utilizing the characteristics of high efficiency and specificity of enzyme preparation for targeted modification of tobacco leaves is a viable method that can be tried to improve the quality of tobacco leaves.
研究领域:
生物学
·
医学
·
烟草科学
✓ 已验证
产品关联
DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2017
·
IF 2.892
Yinzhou Guo,Jiangtao Liu,Kehu Zhang,Honghong Zhang,Yan Li,Zhongli Lei
In this study, a novel type of stimuli-responsive support materials was synthesized successfully by atom transfer radical polymerization (ATRP) and ring opening polymerization (ROP) of N-isopropylacrylamide (NIPAAm), tert -butyl methacrylate (tBMA) and ε-caprolactone (CL). β-Cyclodextrin (β-CD) and azobenzene (Azo) were employed as the starting groups to form PCL-hv-PNIPAAm by host–guest inclusion complexation, while PCL-PMAA was converted by simple hydrolysis of PCL-PtBMA with trifluoroacetic acid. Both PCL-PMAA and PCL-hv-PNIPAAm formed the microspheres by self-assembly. Their characteristics were confirmed by NMR, TEM, GPC, DLS, etc. Then, the microspheres were used for the immobilization of pectinase by electrostatic interaction. The optimal immobilization reaction parameters were 10 U/mL for pectinase at 25 °C for 4 h at pH = 6. Compared with free pectinase, the immobilized enzymes were found to exhibit better tolerance of variations in pH and temperature, as well as improved storage stability. Furthermore, the relative activity of the immobilized pectinase was controlled at approximately 13% when the temperature was higher than the LCST of PNIPAAm. With different light irradiation, there is approximately a 15% influence on the relative activity of the immobilized pectinase. This new enzyme support material has good environmental adaptability and can make the activity of immobilized enzymes be tailored predictably and enable the enzyme to be used as a biocatalyst for various applications in the food industry.
研究领域:
生物化学
·
材料科学
·
食品科学
✓ 已验证
DOI
高影响因子
CHEMOSPHERE
·
2020
·
IF 5.778
Melani Purnika Mudugamuwa Arachchige,Taihua Mu,Mengmei Ma
Novel pectin derived from sweet potato residue was modified by high hydrostatic pressure (HHP)-assisted pectinase and then used for Pb 2+ removal from aqueous solutions. The removal characteristics and mechanisms were also investigated. Results showed that modified sweet potato pectin exhibited greater adsorption performances for Pb 2+ than that of natural ones, and showed excellent eco-friendly properties and good potential for adsorption of some other heavy metals (such as Cu 2+ ). The adsorption curves were much more conformed to Langmuir model, and the highest capacity for Pb 2+ adsorption was 263.15 mg/g with 1.00% pectin at pH 7. Chemical adsorption process of pectin for Pb 2+ absorption involved O-containing functional groups (O–H, COO-), cation exchange, and along with electrostatic interactions. Overall, the results in this study indicated that sweet potato pectin modified with HHP-assisted pectinase had the potential to become an environmentally friendly coagulant-flocculant agent for the heavy metal adsorption, especially for Pb 2+ .
研究领域:
食品科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2016
·
IF 3.138
Gang Fang,Honggao Chen,Yunpeng Zhang,Anqing Chen
Fe 3 O 4 magnetic nanometer particles (MNPS) with a diameter of 30–40 nm and coated by ammoniated silicon dioxides were developed by sol–gel method. The X-ray diffraction (XRD) pattern indicates that the Fe 3 O 4 is wrapped in the amorphous SiO 2 , and the Fourier-transform infrared spectroscopy (FT-IR) analysis confirmed the NH 2 group had been successfully introduced onto the surface of Fe 3 O 4 @SiO 2 nanoparticles. The main factors related to the immobilization process of pectinase onto the magnetic support were optimized by multi-factor orthogonal experiments, and the recommended combination is that 3% glutaraldehyde (coupling agent), pH = 8.0, 50 °C, and cross-linking time 8 h. Scanning electron microscope (SEM) and FT-IR analyses confirmed that the pectinase had been immobilized onto the surface of the amino modified nanoparticles. The storage stability and reusability of the pectinase were enhanced by the immobilization that 64.4% of the total starting activity was retrieved after 7 recycling batches and only about 21.1% of the total activity was lost after 30-day storage. For the application of the immobilized pectinase, the optimal pH and temperature were 8.5 and 55 °C, respectively.
研究领域:
生物化学
·
纳米生物学
·
酶工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）