JOURNAL OF APPLIED MICROBIOLOGY
·
2019
·Vol. 127
·期 4
·1148-1156
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IF 2.683
Yang Y.,OuYang Q.,Li L.,Shao X.,Che J.,Tao N.
AimsThis study investigated the inhibitory effect of glutaraldehyde (GA) on sour rot in citrus fruit and the underlying antifungal mechanism on mycelial growth of the causative pathogen Geotrichum citri‐aurantii.Methods and ResultsGlutaraldehyde exhibited a strong inhibitory effect on G. citri‐aurantii, with a minimum inhibitory and fungicidal concentration (MFC) of 1·00 µl ml−1. In addition, in vivo application of GA (1 × MFC and 5 × MFC) reduced the disease incidence of sour rot in citrus fruit by 60 and 80% respectively. Scanning electron microscopy results revealed that the morphology of G. citri‐aurantii mycelia was greatly altered by GA treatment. Propidium iodide and Calcofluor White Staining revealed that the membrane permeability, rather than the cell wall integrity, of G. citri‐aurantii mycelia was severely disrupted after the addition of GA. Massive accumulation of malonaldehyde and reactive oxygen species as well as an increase in lipoxygenase activity were observed.ConclusionThese results indicate that GA may inhibit the mycelia growth of G. citri‐aurantii through a membrane damage mechanism induced by membrane peroxidation.Significance and Impact of the StudyGlutaraldehyde is expected to be a novel fungicide for controlling sour rot in citrus fruit.
研究领域:
植物病理学
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食品科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2020
·Vol. 100
·期 10
·3812-3821
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IF 2.614
Ou Chen,Lanhua Yi,Lili Deng,Changqing Ruan,Kaifang Zeng
BACKGROUND Penicillium digitatum is one of the most important pathogens causing citrus green mold, leading to significant economic losses. Traditionally, synthetic fungicides are used to control diseases. However, the side effects of fungicides should not be ignored. Thus, antagonistic yeasts were proposed to be safe and effective alternatives for managing diseases. Orchards are excellent sources of naturally occurring antagonists against pathogens. Therefore, in the present study, antagonistic yeasts obtained from orchards were screened, and the possible biocontrol mechanisms of the most promising yeast were investigated. RESULTS Seventy-eight isolates of yeasts (15 species of 10 genera) were obtained from citrus orchards. In in vitro assays, 16 strains showed antifungal activity against Pichia digitatum and 15 strains showed biocontrol potential against green mold on Olinda oranges. Pichia galeiformis (BAF03) exhibited the best antagonistic activity against P. digitatum during 6 days storage at 25 °C and a good antagonistic activity during 29 days at 4 °C. Pichia galeiformis (BAF03) could colonize and amplify quickly in wounded citrus. Scanning electron microscopy results showed that the citrus wound was colonised by the yeast. A total of eight volatile organic compounds (VOCs) were identified by gas chromatography-mass spectrometry The VOCs produced by P. galeiformis (BAF03) efficiently inhibited P. digitatum . CONCLUSION Pichia galeiformis (BAF03) isolated from a citrus orchard showed potential to control postharvest green mold of citrus. The possible mechanisms of action likely include competition for space and nutrients as well as production of VOCs.
研究领域:
植物病理学
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微生物学
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农业科学
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DOI
PubMed
高影响因子
PLANT CELL REPORTS
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2025
·Vol. 44
·期 2
·1-15
·
IF 4.5
Li Xue,Zhou Yue,Chen Hang,Guo Zetian,Zhang Jinlian,Chen Wenli
Key message The three SDEs of C Las were expressed in citrus leaves by AuNPs-PEI mediated transient expression system, and promoted the proliferation of C Las and inhibited citrus immunity. Huanglongbing (HLB) is the most severe bacterial disease of citrus caused by Candidatus Liberibacter asiaticus ( C Las). C Las suppress host immune responses and promote infection by sec-dependent effectors (SDEs), thus insight into HLB pathogenesis is urgently needed to develop effective management strategies. In this study, we focused on the roles of SDE4310 , SDE4435 and SDE4955 in citrus. We found that the expression of SDE4310 , SDE4435 and SDE4955 to increase with increasing citrus immune genes CsPR1 , CsPR2 , CsPR5 , CsNPR1 , CsRBOHD , CsMAP3K and CsBIK1 , suggesting that the level of citrus immunity could be judged by the expression of SDE. To further explore the relationship between these three SDEs and citrus immunity, we established a transient expression system in citrus leaves, using gold nanoparticle-polyethyleneimine (AuNPs-PEI) to deliver recombinant plasmid containing SDE4310 , SDE4435 or SDE4955 respectively into citrus leaves. Results showed that SDE4310 , SDE4435 and SDE4955 were successfully expressed in citrus leaves using this transient expression system, and found that SDE4310 , SDE4435 and SDE4955 could promote the C Las proliferation by decreasing the immune gene expression of the citrus. Additionally, we used AuNPs-PEI to deliver siRNA 4310 to citrus cells, significantly reducing the expression of SDE4310 within 3 days. Although the suppression of SDE4310 expression did not inhibit the C Las proliferation, it increased the expression level of CsPR1 , CsNPR1 and CsBIK1 . This is also the first time that AuNPs-PEI has been found to be able to deliver exogenous plasmids into citrus cells and express the target protein, providing a new method for future studies on citrus HLB.
研究领域:
植物病理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2020
·
IF 2.877
Yongquan Liu,Ahui Xue,Siyu Wang,Yingbin Hao,Meng Cui,Lihua Liu,Liping Luo
Asian citrus psyllid (ACP) causes direct and indirect damage to the citrus industry. Extractive electrospray ionization mass spectrometry (EESI-MS) and high performance liquid chromatography (HPLC) were used to detect the metabolites of C. limon leaves at 0, 12, 24, and 72 h after ACP treatment. The EESI-MS results showed that ACP infestation significantly affected metabolites within a short feeding duration with 8 metabolites identified. The metabolites in leaves of these four groups could be distinguished, with 55 peaks showing significant differences including methyl N -methylanthranilate, caffeic acid , and syringic acid . The quantification of 15 phenolic compounds with HPLC-UV method in C. limon leaves after ACP infestation showed that the total content of them reached a peak of 3504.69 μg g −1 at 12 h, with 9 phenolic compounds changing significantly ( P < 0.05). A total of 21 metabolites identified in this study were involved in the biosynthesis pathways of flavonoid , flavone and flavonol , isoflavonoid and phenylpropanoid , and the degradation of aminobenzoate . Contents of epicatechin and caffeic acid increased with the feeding time of ACP as detected by both EESI-MS and HPLC. This may be related to plant defense. This study provides novel insights into the biochemical relationship of ACP and its host plants.
研究领域:
植物学
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昆虫学
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代谢组学
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DOI
高影响因子
JOURNAL OF FOOD SCIENCE
·
2025
·Vol. 90
·期 9
·e70540
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IF 3.4
Yimei Zhao,Xinrui Xiao,Jiadai Tang,Xueqiu Wang,Cheng Wu,Lu Qiao
By using non-targeted metabolomics technology and high-throughput sequencing methods, the differences in metabolites between the green and red Citrus reticulata “Chachi” peels (CRCP), and their associations with microbial communities were systematically analyzed. Combined with physicochemical indicators (browning degree, total flavonoid content, antioxidant capacity), the material basis of their quality and functional components was explored. The results showed that the total flavonoid content (21.89 mg/g) and the antioxidant indicators (ABTS, FRAP) in green CRCP were significantly higher than those in red CRCP ( P < 0.05), while there was no significant difference in browning degree. Metabolomics analysis showed that a total of 1,322 differential metabolites were identified in green and red CRCP. A total of 723 metabolites in green CRCP were significantly up regulated (∼54.70%) ( p < 0.05), including active components such as lariciresinol, ginsenoside Rb1, and salidroside, with potential anti-diabetic, anti-tumor and anti-depression properties, respectively. Correlation analysis of physicochemical indicators and metabolites indicated that antioxidant capacity was positively correlated with most metabolites (83.56%), and the accumulation of core metabolites such as dodecatetraene and genipin was synergistically regulated by FRAP, ABTS, and total flavonoids. The abundance of fungi such as Aspergillus spp. In green CRCP was relatively high, which was positively correlated with the total flavonoid and antioxidant indicators, driving the synthesis of flavonoids. Cladosporium in red CRCP was negatively correlated with metabolites, and bacteria were negatively correlated with antioxidant indicators. This study reveals the advantages of green CRCP in antioxidant activity, and provides a theoretical basis at the metabolite and microbial levels.
研究领域:
食品科学
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代谢组学
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微生物学
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天然产物化学
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植物生理学
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产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2022
·Vol. 79
·期 2
·811-820
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IF 4.462
Jing Gao,TongLai Tao,Steven P. Arthurs,Fengxian Ye,Xincheng An,Mubasher Hussain,Runqian Mao
Background Herbivores may influence each other directly and through plant mediated inter-specific interactions. The Asian citrus psyllid ( Diaphorina citri Kuwayama) and citrus aphids are key pests that can co-exist on citrus, but their plant-mediated interaction between them is unknown. Here we investigated plant-mediated effect of two citrus aphid species, the polyphagous Aphis spiraecola Patch and the oligophagous Aphis ( Toxoptera ) citricidus (Kirkaldy) on the feeding behavior and reproduction of Diaphorina citri , and explored the underlying mechanisms. Results In comparison with those on aphid free plants, Diaphorina citri had decreased reproduction and reduced phloem feeding on Aphis spiraecola pre-infested plants, while the reproduction and feeding efficiency were increased on Aphis citricidus pre-infested plants. Jasmonic acid (JA) dependent defense was significantly activated by Diaphorina citri feeding on Aphis spiraecola pre-infested plants, but was suppressed by Diaphorina citri feeding on Aphis citricidus pre-infested plants compared with that on aphid free plant. By contrast, only one tested marker gene in salicylic acid (SA) signaling was activated by Diaphorina citri feeding on Aphis spiraecola pre-infested plants. Furthermore, exogenous application of methyl jasmonate, but not SA, conferred resistance against Diaphorina citri in our citrus trials. Conclusion Our results indicate that pre-infestation by two citrus aphid species differentially altered Diaphorina citri induced citrus JA dependent defense, which resulted in different effect on subsequent Diaphorina citri performance. © 2022 Society of Chemical Industry.
研究领域:
植物科学
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昆虫学
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植物与害虫互作
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分子植物病理学
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产品关联
DOI
PubMed
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2019
·
IF 3.927
Lu Li,Xu Tang,Qiuli Ouyang,Nengguo Tao
Sodium dehydroacetate (SD) and sodium silicate (Na 2 SiO 3 ) are food preservatives with antifungal activities against many pathogens. In this paper, the antifungal activity of the combination treatment of SD and Na 2 SiO 3 against Geotrichum citri-aurantii , the postharvest pathogen of sour rot in citrus fruit, was tested. SD and Na 2 SiO 3 combination (1:4, w/w) showed a synergistic inhibitory effect compared to single compound on the mycelial growth of G. citri-aurantii , with the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) being 1.8 and 3.6 g L −1 , respectively. The spores’ germination and the plasma membrane integrity of the pathogen could also be visibly influenced by the 1× MFC SD + Na 2 SiO 3 treatment. The application of various SD + Na 2 SiO 3 concentrations (1×, 5×, and 10× MFC) to citrus fruit inoculated with G. citri-aurantii significantly ( P < 0.05) decreased the incidence of sour rot during the entire storage period. After 8 d of storage, the disease incidences in SD + Na 2 SiO 3 (10×, 5× or 1× MFC)-treated fruit were only 20%, 30%, 65%, respectively, in contrast to 100% of the control fruit. Meanwhile, SD + Na 2 SiO 3 treatments maintained the fruit quality of citrus fruit. Results indicate that the combination of SD and Na 2 SiO 3 is a promising approach in controlling the postharvest decay of citrus fruit.
研究领域:
食品科学
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植物病理学
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采后技术
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产品关联
DOI
高影响因子
Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment
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2019
·
IF 2.17
Xiangwu Liu,Ya Yang,Ya Chen,Qingtao Zhang,Ping Lu,Deyu Hu
An efficient, sensitive, simple and fast method for the simultaneous determination of oxine-copper and pyraclostrobin in citrus fruit was developed and validated. The method uses ethylene diamine tetra-acetic acid as a competitive ligand to convert oxine-copper to soluble 8-hydroxyquinoline for analysis by QuEChERS and LC-MS/MS. Linear relationships were determined with correlation coefficients ranging from 0.9904 to 0.9998. The limits of detection for the analytes were 0.012–0.8 μg kg−1, and the limits of quantitation were 0.04–2.6 μg kg−1 in citrus. The average recoveries were 79.1–114.9% with relative standard deviations of less than 7.4%. The analyses of dissipation indicated that the half-lives of oxine-copper and pyraclostrobin were 1.94–3.67 and 1.79–2.48 days and the terminal residues were <0.08–8.99 and <0.02–1.90 mg kg−1, respectively. The risk quotients of oxine-copper and pyraclostrobin were 0.026–0.199 and 0.003–0.022, respectively. This risk assessment provides a reference for the safe and reasonable use of oxine-copper and pyraclostrobin and may help to establish maximum residue limits for these pesticides in China.
研究领域:
农业科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2014
·
IF 2.628
Xingfeng Shao,Baoying Cao,Feng Xu,Shuhui Xie,Dandan Yu,Hongfei Wang
The antifungal activity of chitosan combined with clove oil against Penicillium digitatum , the causal agent of citrus green mold, was tested in vitro and in vivo . Chitosan combined with clove oil inhibited mycelial growth more than individual treatments, which was related to the greater release of cellular material and the largest alterations in hyphal morphology of P. digitatum . However, compared to chitosan alone, 1% chitosan coatings combined with various amounts of clove oil (0.5, 1 or 2 mL/L) showed no greater ability in controlling decay development on artificially inoculated citrus fruit. 1% chitosan combined with 0.5 mL/L clove oil appeared to slightly reduce lesion diameter and enhanced the activities of defense enzymes, including chitinase and phenylalanine ammonia-lyase at the later stages of incubation. This study indicated that the synergistic antifungal activity of chitosan-clove oil observed in in vitro studies was not found in in vivo tests. Therefore, the data suggest that a coating of 1% chitosan alone, not combined with clove oil, can effectively contribute to the control of green mold on citrus fruit.
研究领域:
植物保护
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食品科学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2015
·
IF 4.097
Jing Yu,Na Song,Ya-Kun Zhang,Shu-Xian Zhong,Ai-Jun Wang,Jianrong Chen
Green, low-cost, and water-soluble fluorescent carbon dots were prepared under hydrothermal conditions, with a plant of Jinhua bergamot as a carbon source. The as-synthesized C-dots have better stability and relatively high photoluminescence with a quantum yield of 50.78%, along with the fluorescence lifetime of ca. 3.84 ns. Their photoluminescence can be significantly quenched by simply using the buffer solution of HAC–NaAC for Hg 2+ and Tris–HCl for Fe 3+ . Based on this, we explored for selective detection of Hg 2+ and Fe 3+ with high selectivity, fast response, low cost, and broad linear ranges of 0.01–100 μM for Hg 2+ and 0.025–100 μM for Fe 3+ , as well as low detection limits of 5.5 nM (Hg 2+ ) and 0.075 μM (Fe 3+ ) (S/N = 3).
研究领域:
化学
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环境科学
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材料科学
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产品关联
DOI
高影响因子
Separations
·
2024
·Vol. 11
·期 4
·95
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IF 2.5
Chenyang Yao,Yachao Li,Peng Li,Xiaokui Che,Guosheng Li,Fanfan Zhang,Weijun Peng,Bozeng Wu,Guixia Fan
This paper presents the development of an environmentally friendly, small molecular depressant citrus pectin for improving the recovery of cassiterite resources. Citrus pectin extracted from citrus peel was utilized as the depressant, and it demonstrated significant potential in separating calcite from cassiterite in micro-flotation tests. The molecular weight of the citrus pectin extracted in this paper decreased from 11,485,412 Da to 32,959 Da compared to commercial pectin, resulting in the depressant efficiency of the reagent. The results of a zeta potential and adsorption test indicated that citrus pectin had less and weaker adsorption on the cassiterite surface and could be replaced with NaOL. The chemical adsorption process of citrus pectin on the surface of calcite was determined through FTIR spectroscopy analysis. XPS analysis results indicated that the interaction between the carboxyl groups of citrus pectin and calcium atoms enables adsorption to occur. The AFM revealed that citrus pectin displayed a uniform and dense pattern of point-like adsorption on the surface of calcite. Micro-flotation experiments showed that cassiterite recovery of 80% can be obtained at a citrus pectin dosage of 10 mg/L. Citrus pectin has the advantages of being low-cost, highly selective, and environmentally suitable, making it a promising alternative to conventional reagents.
研究领域:
矿物加工工程
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环境科学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
PEST MANAGEMENT SCIENCE
·
2025
·
IF 3.8
Tian-Yuan Liu,Yu-Xi Zhou,Chen-Yang Yuan,Yi-Fan Gao,Jia-Yao Fan,Guo-Rui Yuan,Jin-Jun Wang,Wei Dou
BACKGROUND Diaphorina citri , a serious pest of citrus, causes significant economic losses due to its role in spreading Huanglongbing. Chemical treatment is the primary method for controlling D. citri . In recent years, D. citri has demonstrated varying resistance levels to a number of pesticides. Combination bioassay and amplicon sequencing approaches can provide a more accurate assessment of resistance in D. citri . RESULTS Field populations of D. citri exhibited significant variability in pesticide susceptibility depending on geographic location. The Guilin population showed the highest imidacloprid sensitivity, with a median lethal concentration (LC 50 ) of 1.14 mg L −1 , indicating a comparatively high susceptibility to this pesticide. In contrast, the Chongzuo population exhibited the lowest sensitivity, with an LC 50 exceeding 2000 mg L −1 , reflecting a significant resistance level. Molecular analysis showed that resistance levels were associated with specific genetic mutations in two key areas: VGSC_L925M, VGSC_M918T, and the acetylcholine receptor α (nAChRα_N88S). Furthermore, there was a correlation between the rate of these mutations and the resistance level. Interestingly, the VGSC_L925M mutation was prevalent in the Chongzuo population, exhibiting a significant frequency of 42.08%. The nAChRα_N88S mutation, found at a low frequency of 0.24%, was unique to the Chongzuo population. CONCLUSION The frequency of VGSC_L925M mutations linked to pyrethroid resistance and nAChRα_N88S mutations associated with neonicotinoid resistance has been revealed in D. citri from major citrus-producing regions in China. We recommend region-specific resistance management strategies, immediate reduction of pyrethroid use in Chongzuo area with high L925M mutation frequencies. This research also provides fundamental data to manage resistance in D. citri . © 2025 Society of Chemical Industry.
研究领域:
昆虫学
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分子生物学
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农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）