TALANTA
·
2024
·
IF 5.6
Kai Zhang,Yule Qian,Chaoyan Lou,Mingli Ye,Yan Zhu
PyICLab is an open-source Python-based package featuring an object-oriented programming (OOP) interface, providing tools for realistic and customized numerical simulations of ion chromatography (IC). In this paper, we showcase PyICLab's use in simulating diverse separation scenarios, including isocratic carbonate elution, gradient hydroxide elution, high-concentration and large-volume injections. The accuracy of the embedded models was validated by demonstrating strong correlations between predicted and experimental results. Additionally, PyICLab's capability to handle complex IC configurations was demonstrated through a simulation of a column-switching system for seawater analysis. PyICLab offers valuable resources for chromatographic optimization, method development, and educational purposes. It is available on PyPI at pypi.org/project/pyIClab. Interested readers can install PyICLab using the pip command in a Python 3.11 or higher environment.
研究领域:
分析化学
·
计算科学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
·
2017
·
IF 0.697
Qiqi Wu,Zhiwei Lin,Haibao Zhu,Chengzhu Ni,Meilan Chen
In this work, a liquid chromatography connected with an ion chromatography by the column-switching technique was developed for the simultaneous determination of three phytochemicals (scopoletin, rutin, and quercetin), three anions (chloride, sulfate, and phosphoric), and seven organic acids (lactic, acetic, propionic, formic, malic, oxalic, and citric acid) in Chinese traditional herbs, S. japonica bud and premium noni juice. A concentration column was used to collect organic acids and anions, and then the concentrated analytes were eluted to an analytical column by column-switching technique in which target analytes could be separated successfully and detected simultaneously within 70 min. This method showed good linearity with the linear correlation coefficients in the range of 0.9971–1.0000. The relative standard deviations of the peak areas were all less than 5.63% (n = 6). The limits of detection (LOD, S/N = 3) of anions and organic acids were in the range of 0.0006–0.0857 mg/L, whereas phytochemicals were in the range of 0.0123–0.0197 mg/L. Recoveries were in the range of 86.53–117.01% for the spiked sample. This method was successfully applied to the determination of the low-weight organic acids and phytochemicals in Chinese traditional herbal medicines, S. japonica bud and premium noni juice.
研究领域:
分析化学
·
中药学
·
植物化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2017
·
IF 3.981
Nadeem Muhammad,Qamar Subhani,Fenglian Wang,Dandan Guo,Qiming Zhao,Shuchao Wu,Yan Zhu
This work illustrates the introduction of a simple, rugged and flexible column-switching ion chromatography (IC) technique for an automated on-line QuEChERS extracted samples extracts washing followed by sensitive fluorescence (FLD) determination of five acidic pharmaceutical drugs namely; clofibric acid (CLO), ibuprofen (IBU), aspirin (ASP), naproxen (NAP) and flurobrofen (FLU) in three complex samples (spinach, apple and hospital sewage sludge). An old anion exchange column IonPac ® AS11-HC was utilized as a pre-treatment column for on-line washing of inorganic and organic interferences followed by isocratic separation of five acidic drugs with another anion exchange IonPac ® AS12A analytical column by exploiting the column-switching technique. This novel method exhibited good linearity with correlation coefficients (r 2 ) for all drugs were in the range 0.976–0.996. The limit of detection and quantification of all five acidic drugs were in the range 0.024 μg/kg to 8.70 μg/kg and 0.082 μg/kg to 0.029 mg/kg, respectively, and better recoveries in the range 81.17–112.5% with percentage relative standard deviations (RSDs) less than 17.8% were obtained. This on-line sample pre-treatment method showed minimum matrix effect in the range of 0.87–1.25 except for aspirin. This simple rugged and flexible column-switching system required only 28 min for maximum elimination of matrices and interferences in three complex samples extracts, isocratic separation of five acidic drugs and for the continuous regeneration of pre-treatment column prior to every subsequent analysis. Finally, this simple automated IC system was appeared so rugged and flexible, which can eliminate and wash out most of interference, impurities and matrices in complex samples, simply by adjusting the NaOH and acetonitrile concentration in washing mobile phase with maximum recoveries of acidic analytes of interest.
研究领域:
分析化学
·
环境科学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Yang Yang,Wei Ma,Baiyang Chen,Chong Peng,Wang Luo,Huan He
Haloacetic acids (HAAs) are a group of disinfection byproducts ubiquitously found in disinfected drinking water and they are currently regulated by some governments. Conventional HAAs analytic methods employ either gas chromatography (GC), two-dimensional ion chromatography (2D-IC), or ion chromatography with electrospray ionization tandem mass spectrometry (IC-ESI-MS/MS) to reduce the effect of interfering compounds and enhance detection resolution; however, they are either laborious in sample preparation procedures or too expensive to be used by common laboratories. To bypass these problems, this study proposes to analyze HAAs by employing a one-pump column-switching ion chromatography (OPCS-IC) that allows direct sample injection without requiring extraction and derivatization pretreatments. Unlike 2D-IC method, which uses two pumps and two parallel sets of IC columns, this method applies only one pump and one column. The tailored OPCS-IC removes coexisting anions from water and enriches HAAs simultaneously, thereby distinguishing HAAs from interfering anions and magnifying the signals of HAAs. For example, when HAAs were enriched by five times, the coexisting F − , Cl − , Br − , NO 3 – , and SO 4 2− were removed by 62.4%, 93.9%, 99.7%, 99.9%, and 98.9%, respectively. Meanwhile, the recoveries of HAAs dosed into real samples ranged from 84% to 102%, similar to those obtained from conventional GC method, meaning that the convenience of this method did not compromise its performance. The study hence proves an easy-to-use and relatively cheaper method for measuring HAAs in drinking water.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2012
·
IF 4.531
Zhongping Huang,Zuoyi Zhu,Qamar Subhani,Wenwu Yan,Weiqiang Guo,Yan Zhu
A simple ion chromatographic method for simultaneous detection of iodide and iodate in a single running was proposed, with columns packed with homemade functionalized polystyrene-divinylbenzene (PS-DVB) resins and column-switching technique. Homemade resins were functionalized with controllable amounts of quaternary ammonium groups. The low-capacity anion-exchange column and high-capacity anion-exchange column were prepared, due to the resins having different exchange capacities. With this method, iodide and iodate in povidone iodine solution were detected simultaneously in a short time with iodide being eluted off first. A series of standard solutions consisting of target anions of various concentrations from 0.01 mg/L to 100 mg/L were analyzed. Each anion exhibited satisfactory linearity, with correlation coefficient r ≥ 0.9990. The detection limits (LODs) for iodide and iodate obtained by injecting 100 μL of sample were 5.66 and 14.83 μg/L (S/N = 3), respectively. A spiking study was performed with satisfactory recoveries between 101.2% and 100.6% for iodide and iodate.
研究领域:
分析化学
·
医学检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Water
·
2022
·Vol. 14
·期 15
·2371
·
IF 3.53
Huili Tang,Ziru Li,Hanqing Chen,Yang Xu,Xu Jiang,Erdeng Du,Zhen Lyu,Lu Zheng,Mingguo Peng
N-nitrosamines have recently attracted attention as a class of disinfection byproducts and are also a hot spot in environmental studies. Current N-nitrosamine analytical methods typically involve manual solid phase extraction (SPE) of samples followed by quantitative analysis using liquid chromatography-mass spectrometry (LCMS), which is time-consuming and may also fail to eliminate complex matrix effects. Size exclusion chromatography (SEC) is a technique that can separate compounds according to their molecular size. For the first time, this study developed an Online-SPE/SEC/LCMS quantitative analysis method to detect and analyze nine common N-nitrosamine disinfection byproducts in wastewater plant tailwater, including N-dimethylnitrosamine (NDMA) and N-nitrosodiethylamine (NDEA), etc. The samples of 1.0 mL can be directly injected after the simple 0.22 μm membrane filtration. This method reports the combination of SPE, SEC, and RP C18 columns to achieve several functions in a processing time of 20 min, including online enrichment, desalination, and matrix separation for the first time. The method provides good linearity (R2> 0.999), recoveries ranging from 91.67% to 105.88%, relative standard deviation (RSD) lower than 4.17%, and the limits of detection (LOD) are 0.12–6.60 ng/L. This method alleviates tedious human labor and can effectively overcome the matrix effect (ME< 20%). This method allows for the accurate quantitative analysis of N-nitrosamines with high compatibility in wastewater plant tailwater, rivers, and lakes with a high background matrix. Interested researchers can also use this method as a reference in the online analysis of other specific pollutants after necessary optimization. It can also be utilized for non-targeted screening and targeted analysis of contaminants in water with a wide range of applications, giving valuable information for environmental monitoring.Keywords:N-nitrosamines;analysis method;online solid-phase extraction (Online-SPE);size exclusion chromatography (SEC);LCMS;column switching;wastewater plant tailwater
研究领域:
环境科学
·
分析化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）