Reproductive Biology and Endocrinology
·
2023
·Vol. 21
·期 1
·1-8
·
IF 4.4
Qiaomei Zheng,Ping Wu,Yanjing Zhao,Jinhua Wang,Shaozhan Chen,Lihong Chen
Background Emerging evidence of immunological dysfunction have been described in endometriosis. Dendritic cells (DCs), one of the main antigen-presenting cells, are specialized in the initiation and modulation of the adaptive immune response. Emerging studies demonstrated both endometrial and circulating differences in DCs populations in women with endometriosis. However, the role and mechanism of peritoneal DCs in endometriosis is still unclear. The present study was undertaken to explore the features of peritoneal DCs in the pathogenesis of endometriosis. This study is beneficial to further clarify the cause of endometriosis and provide a new insight into the medical treatment for endometriosis.Methods The study included 12 women with endometriosis and 11 women without endometriosis. The C57BL6 mouse model of endometriosis was established by intraperitoneal injection of endometrial segments. The peritoneal DCs of endometriosis patients and mouse models were analyzed by fluorescence associated cell sorting (FACS) examination.Results Increased cell density of peritoneal DCs were observed in endometriosis patients. Moreover, the proportion of mature DCs (mDCs, CD80highCD1alow cells) in the peritoneal DCs was lower whereas the proportion of immature DCs (iDCs, CD80lowCD1ahigh cells) was increased in endometriosis patients. Similarly, the cell density of peritoneal DCs in murine models increased immediately after the injection of endometrial tissues and reached the highest level at 14 days. In addition, the proportion of mDCs (CD11chighCD80high cells) in the peritoneal DCs decreased immediately after the injection of endometrial tissues and then increased with the time until 42 days, but still lower than the control group. In contrast, the proportion of iDCs (CD11chighCD80low cells) in the peritoneal DCs showed the opposite dynamic changes. However, after treated with LPS, the mDCs proportion was significantly increased, leading to lower volume and weight of the endometriosis lesions.Conclusion sIncreased level of peritoneal DCs facilitated the pathogenesis of endometriosis lesions, especially in the early stage of the disease. Furthermore, peritoneal DCs maturation played an important role in the development of endometriosis.
研究领域:
妇产科学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEURON
·
2023
·
IF 16.2
Ning Cheng,Qiqi Dong,Zhen Zhang,Li Wang,Xiaojing Chen,Cheng Wang
Egocentric representations of external items are essential for spatial navigation and memory. Here, we explored the neural mechanisms underlying egocentric processing in the retrosplenial cortex (RSC), a pivotal area for memory and navigation. Using one-photon and two-photon calcium imaging, we identified egocentric tuning for environment boundaries in dendrites, spines, and somas of RSC neurons (egocentric boundary cells) in the open-field task. Dendrites with egocentric tuning tended to have similarly tuned spines. We further identified egocentric neurons representing landmarks in a virtual navigation task or remembered cue location in a goal-oriented task, respectively. These neurons formed an independent population with egocentric boundary cells, suggesting that dedicated neurons with microscopic clustering of functional inputs shaped egocentric boundary processing in RSC and that RSC adopted a labeled line code with distinct classes of egocentric neurons responsible for representing different items in specific behavioral contexts, which could lead to efficient and flexible computation.
研究领域:
神经科学
·
认知神经生物学
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系统神经科学
·
计算神经科学
·
行为神经科学
·
分子与细胞神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Antioxidants
·
2025
·Vol. 14
·期 4
·468
·
IF 6.6
Xin Zhang,Shuang Han,Zhu Zeng,Jie Dai,Yi Jia
Selenium-binding protein 1 (SELENBP1) has been implicated in cancer development, neurological disorders, tissue injury, metabolic regulation, and cell differentiation. Sepsis is characterized prominently by immunological dysregulation and severe organ damage. However, whether SELENBP1 improves sepsis by regulating immune cell activity remains unknown. Here, we detected an elevation of SELENBP1 levels in the blood of sepsis patients and in the livers of septic mice. Significantly, SELENBP1 knockout (KO) prolonged survival in septic mice. This phenomenon was accompanied by decreased liver damage, reduced inflammation levels, and an increased regulatory T cell/T helper 17 cell (Treg/Th17) ratio in the spleen. Additionally, SELENBP1 deficiency induced a redox imbalance and inhibited dendritic cell (DC) maturation, resulting in a tolerogenic DC (tolDC) phenotype and an increase in the Treg/Th17 ratio. Furthermore, SELENBP1-KO mature DCs (mDCs) alleviated liver injury by increasing the Treg/Th17 ratio in the spleen, thus improving the survival of septic mice. These findings indicate that SELENBP1 is involved in sepsis by regulating DC immune activity, which might provide a potential way for sepsis treatment.
研究领域:
免疫学
·
医学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Abubakar Abdussalam,Hongzhan Liu,Islam Mohamed Mostafa,Baohua Lou,Dmytro Viktorovych Snizhko,Yuriy Tymofiiovych Zholudov,Wei Zhang,Guobao Xu
Chemiluminescence (CL)-based analytical methods utilize luminophores that need to be activated with an oxidizing agent to trigger CL emission. Despite its susceptibility to decomposition when exposed to external light or trace metals, hydrogen peroxide (H2O2) has been widely used to develop chemiluminescent methods due to the limited number of suitable alternatives for activating chemiluminescent luminophores. Also, analytical methods based on the well-known luminol/H2O2 CL system have low sensitivity. Dissolved oxygen (DO) is a naturally abundant and environmentally benign alternative oxidant for luminol and other CL luminophores. However, DO alone is inactive and needs an efficient catalyst or a coreaction accelerator for its activation. Because of the narrow bandgap of VS4 (ca. 1.12 eV), it can facilitate fast electron-transfer kinetics with an acceptor molecule such as DO. Here, we introduce vanadium tetrasulfide (VS4) to boost CL for the first time. Under the optimized conditions, VS4 nanodendrite catalyzes the generation of reactive oxygen species by activating DO which subsequently reacts with luminol to generate intense CL. It enhances the CL intensity of luminol/DO by about 10,000 times. Surprisingly, hemin remarkably quenches the generated CL of luminol/DO/VS4 nanodendrites, which is completely opposite to its typical enhancement of luminol CL. Based on the remarkable concentration-dependent quenching of the luminol/DO/VS4 nanodendrite CL by hemin, we have developed a sensitive CL method that can selectively detect hemin in the linear concentration range of 1–250 nM and achieved a limit of detection of 0.11 nM. The practical utility of the developed method was demonstrated by the determination of hemin in a pharmaceutical drug for the treatment of acute intermittent porphyria and in human serum. This study demonstrates that VS4 holds great promise in analytical method development.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INFLAMMATION
·
2020
·Vol. 43
·期 5
·1780-1788
·
IF 3.212
Zhang Liming,Huang Yang,Cui Xiaoxiao,Tan Xiao,Zhu Ying,Zhou Wenjun,Wang Chaokui,Yuan Gangxiang,Cao Qingfeng,Su Guannan,Kijlstra Aize,Yang Peizeng
Previous studies have pointed out that indoleamine 2,3-dioxygenase (IDO), the rate-limiting enzyme initiating tryptophan metabolism, plays a role in the regulation of the immune system. This project was designed to investigate the potential role of IDO in monocyte-derived dendritic cells (moDCs) obtained from active Vogt-Koyanagi-Harada (VKH) disease patients. In this study, we found that the IDO mRNA expression and enzyme activity were increased in active VKH patients as compared with healthy controls and patients in remission. To investigate the role of IDO in immune regulation, an effective inhibitor 1-methyl-L-tryptophan (1-MT) was used to suppress its activity in DCs. The results showed that inhibition of IDO with 1-MT significantly decreased the expression of DC marker CD86. IDO inhibition did not affect the cytokine production of IL-6, IL-1β, TNF-α, IL-10, and TGF-β in DCs. Downregulation of IDO in DCs also led to the reduction of regulatory T (Treg) cells and an increased CD4 + T cell proliferation. Treatment with 1-MT did not affect the phosphorylation of the MAPK pathway in DCs. In general, our study suggests that IDO may play an important role in the pathogenesis of VKH disease by regulating DC and CD4 + T cell function. Tryptophan deficiency and kynurenine accumulation may account for the complicated effects of IDO. Further research is needed to study the precise tryptophan metabolites that may limit immune responses in VKH disease.
研究领域:
免疫学
·
眼科
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Energy Storage Materials
·
2017
·
IF 0
Liping Wang,Lei Zhang,Qingji Wang,Wenjun Li,Bo Wu,Weishang Jia,Yuehui Wang,Jingze Li,Hong Li
Lithium metal is a Holy Grail anode for high energy density lithium ion batteries . While, its high chemical reactivity and high tendency to form dendrites hinder its wide applications. Here, in this study, we stabilize lithium metal by engineering an artificial SEI layer, namely fabricating a conformal nano-meter thickness amorphous Al 2 O 3 thin film via magnetron sputtering technique on its surface. The obtained Al 2 O 3 modified Li metals present robust chemical surface stability and effectively restrain the dendrite growth. 20 nm-thick Al 2 O 3 film modified lithium metal in an all-solid-state battery Li/PEO-LiTFSI/Li ensures a cycle life of 660 h at a current density of 0.1 mA/cm 2 . Contradicting to a traditional belief of lithium plating at the current collector side via crossing the Al 2 O 3 film, it directly plates on the Al 2 O 3 film next to the separator side. Al 2 O 3 film plays a crucial role as a “surfactant” in inducing a layer-by-layer film growth mode.
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
Nanomaterials
·
2023
·Vol. 13
·期 21
·2855
·
IF 5.3
Shan Wang,Lifeng Ma,Dan Song,Shengchun Yang
To boost the electrocatalytic methanol oxidation reaction (MOR) of Platinum (Pt), making binary PtM (M = transition metals, for example, Fe, Cu, and Ni) with specific morphology is known as a promising method. Although great progress has been made in the synthesis of shaped PtM catalysts toward MOR, enhancing the catalytic performance of the PtM to enable it to be commercialized is still a hotspot. In this work, the Au-doped PtNi dendritic nanoparticles (Au-PtNi DNPs) were obtained by doping a small amount of gold (Au) into initially prepared PtNi DNPs, greatly improving their MOR catalytic activity and durability. The energy-dispersive X-ray spectroscopy mapping (EDXS) indicates that the surface of DNPs is mainly composed of Au dopant and PtNi, while the core is mainly Pt, indicating the formation of Au-doped PtNi/Pt core-shell-like DNP structures. The electrocatalytic performance of the prepared Au-PtNi DNPs with different compositions for the MOR was evaluated using cyclic voltammetry, chronoamperometry, and CO-stripping tests. The experimental findings indicate that the Au-PtNi DNPs showed better MOR performance in comparison with PtNi DNPs and commercial Pt catalysts. Among all the catalysts, 6% Au-PtNi DNPs showed 4.3 times improved mass catalytic activity for the MOR in comparison with commercial Pt catalysts. In addition, all the prepared Au-PtNi DNPs display a remarkable CO tolerance compared to that of PtNi DNPs and commercial Pt catalysts. The dendritic structure of Au-PtNi DNPs can effectively enhance catalytic performance, combined with the electronic effect of Au, Pt, and Ni.Keywords:Au dopant;nanodendrite;AuPtNi shell;methanol oxidation reaction
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2018
·Vol. 5
·期 3
·141-149
·
IF 0
Shen Taipeng,Zhu Wencheng,Yang Li,Liu Li,Jin Rongrong,Duan Jimei,Anderson James M,Ai Hua
Dendritic cell (DC)-based vaccines have shown promising therapeutic results in cancer and some immune disorders. It is critical to track in vivo migration behaviours of DCs and monitor the whole process dynamically and non-invasively. Superparamagnetic iron oxide (SPIO) nanoparticles are chosen for DC labelling under magnetic resonance imaging (MRI) because of their proven biosafety as contrast agents. However, when used for cell labelling, sensitive biological indicators such as cell autophagy may be helpful to better understand the process and improve the probe design. Here, lactosylated N-Alkyl polyethylenimine coated SPIO nanoparticles are used for DC labelling. This probe shows satisfactory cell labelling efficiency and low cytotoxicity. In this study, autophagy was used as a key factor to understand how DCs react to nanoparticles after labelling. Our results demonstrate that the nanoparticles can induce protective autophagy in DCs, as inhibition of the autophagy flux could lead to cell death. Meanwhile, the nanoparticles induced autophagy could promote DC maturation which is an essential process for its migration and antigen presentation. Autophagy induced DC maturation is known to enhance the vaccine functions of DCs, therefore, our results suggest that beyond the MRI tracking ability, this probe might enhance therapeutic immune activation as well.
研究领域:
纳米医学
·
免疫学
·
癌症研究
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
BIOMATERIALS
·
2025
·
IF 12.9
Wen Guo,Zhiqi Wang,Rubing Xue,Xinyue Zhang,Qinfu Zhao,Yikun Gao,Siling Wang,Yuling Mao
Weak response is the bottleneck for subunit prophylactic vaccines to trigger efficient immunization. The emergence of highly effective adjuvants will revolutionize. Considering that genomic epigenetic reprogramming plays an important role in the immune response, we herein present an minimalist epigenetic nano-adjuvant gMSN constructed of β-glucan-modified functional mesoporous silica (MSN), which we term as OVA/gMSN after loaded with the model antigen ovalbumin (OVA). Oral administration of OVA/gMSN ensures that enough antigen reach dendritic cells (DCs) in intestinal mucosal lymphoid follicles via intestinal M cells while relying on gMSN to exhibit good adjuvant properties to trigger an effective systemic co-mucosal immune response. gMSN upregulates genes associated with aerobic glycolysis, promoting metabolic reprogramming in DCs and enhancing the expression of genes related to DC activation, cross-presentation, maturation, and migration. Simultaneously, it increases the accumulation of key epigenetic markers H3K27ac, H3K4me1, and H3K4me3. ATAC-seq results reveal enhanced chromatin accessibility of key immune genes, such as Il2rα1, Il18r1, and Cd83, in gMSN-treated DCs. This integrative molecular mechanism demonstrates that gMSN induces epigenomic remodeling in DCs after oral administration, showcasing the potential of combining epigenetic adjuvants and nanotechnology in oral vaccine design, and providing new directions and a theoretical basis for vaccine adjuvant development.
研究领域:
免疫学
·
纳米医学
·
疫苗学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2017
·
IF 0
Yijun Li,Xinyu Wen,Min Nie,Qi Wang
Dendritic β-nucleating agent (β-NA) can readily manipulate the formation of dendritic β-crystal with a unique toughening effect on polypropylene (PP) to drastically enhance the ductility. However, by the current method, the geometric size is too large to fully perform the nucleating efficiency. In this study, by comparatively investigating the effect of molecular weight of PP and diffusion of β-NAs in a PP melt, we proposed a novel carrier strategy that selective enrichment of β-NAs in a PP carrier was followed by directed migration into polymer matrix. Accordingly, the growth of NAs was controlled by the release from the PP carrier, which decreased the available amount of β-NAs during the growth stage. In this case, the viscosity difference between PP carrier and matrix determined the interfacial movement of β-NAs. When the PP carrier and matrix had same molecular weight, the diffusion and release became favorable to facilitate the formation of the dense and fine dendritic aggregates. As a result, the relative content of β-crystals reached 92%, with a drastic increase of ∼82% in the optimal condition compared to the directed compounded PP/β-NAs sample. This study can open a new avenue to tailor the topologies of β-NAs and the ensuing β-crystals for high-performance PP products.
研究领域:
材料科学
·
高分子化学
✓ 已验证
产品关联
DOI
PubMed
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Hui-Ying Sun,Yu Ding,Ya-Qi Yue,Qi Xue,Fu-Min Li,Jia-Xing Jiang,Pei Chen,Yu Chen
The rational design of advanced electrocatalysts and energy-saving electrolysis strategies is highly desirable for achieving high-efficiency electrochemical H2 generation yet challenging. In this work, we report highly branched Pd hydride nanodendrites (PdH-NDs) formed by a very facial solvothermal method and a succedent chemical H intercalation method in N,N-dimethylformamide. The electrocatalytic performance of PdH-NDs is experimentally and theoretically correlated with the morphology and composition, which has demonstrated substantially enhanced electrochemical activity and stability for formate oxidation reaction and hydrogen evolution reaction in alkaline electrolyte compared with Pd nanodendrites. Density functional theory calculations suggest a downshift of the Pd d-band center of PdH-NDs due to the dominant Pd–H ligand effects that weaken the binding energies of the intermediate catalytic species and toxic carbon monoxide. The asymmetric formate electrolyzer based on bifunctional PdH-ND electrocatalysts is first constructed, which only requires a low voltage of 0.54 V at 10 mA cm–2 for continuous H2 generation. This study reveals significant insights about the morphology/composition-performance relationship for palladium hydrides with bifunctional electroactivity.
研究领域:
材料科学
·
电化学
·
能源科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 43
·19889-19896
·
IF 3.3
Lanfang Wang,Ruifang Ding,Yanqing Hao,Yujia Li,Wenjiao Liu,Wenbo Lu,Xiaohong Xu
A novel light-driven non-enzymatic glucose sensor based on AuNi nanodendrites has been successfully constructed and applied in glucose detection. The AuNi nanodendrites were fabricated through a one-step underpotential electrodeposition approach, in which porous anodic aluminum was introduced as the template. This nanodendritic structure with a large surface area provides abundant active sites and good electron transfer for the oxidation of glucose. Considering the plasmonic effect of Au, such a hierarchical hybrid structure displayed remarkable photoelectrocatalytic activity for glucose under the visible light illumination. As a result, the light-driven glucose sensor based on AuNi nanodendrites reveals a high sensitivity of ∼183 μA cm−2 mM−1 (0.1–18 mM) and a relative low detection limit (0.01 mM), without applying an external electrical power supply. The current work is promising in the design of novel hybrid materials for glucose sensors under visible light illumination without electrical power consumption.
研究领域:
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）