WATER AIR AND SOIL POLLUTION
·
2023
·Vol. 234
·期 2
·1-12
·
IF 2.9
Wang Ke,Huang Changjiang,Tang Jun,Liu Shuai,Miao Yuqing,Liu Yuntong,Wu Chuansheng
Bisphenol A (BPA) reportedly exerts concentration-dependent effects on plant growth, based on hydroponic exposure experiments. However, this pattern might differ in soil substrate, and the effects of different BPA concentrations in soil on crop germination as well as symbiotic relationship with rhizobia remain largely unclear. In this study, we evaluated the effects of different BPA concentrations (0, 10, 20, 40, 80, 160, and 320 mg/kg air-dry soil) on soybean growth index, germination, and root nodule by soil culture experiments. Results showed that low-dose BPA (10 mg/kg) treatment resulted in a 38.0 and 59.4% increase in total biomass and yield, respectively, while germination time was shortened by 10.1%. Conversely, high-dose BPA treatment significantly inhibited total biomass, yield, and germination time by an average of 39.9, 35.4, and 31.3% in 160 and 320 mg/kg, respectively. Root nodule biomass decreased in all BPA treatments, especially significantly decreased evenly by 66.6% in high concentrations of 160 and 320 mg/kg. Furthermore, germination time significantly explained variations of total biomass and yield by 19.2 and 18.3%, whereas nodule biomass significantly explained by 40.4 and 26.2%, respectively. The structural equation modeling revealed that BPA inhibited soybean total biomass and yield by prolonging germination time and inhibiting soybean nodule formation, while nodule biomass exhibited a larger path coefficient (0.553 and 0.475) than germination time (− 0.252 and − 0.280), suggesting a more important role of soybean nodule. Collectively, these findings provide invaluable insights into the mechanisms underlying the effects of BPA on crop growth.
研究领域:
植物学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Shijin Xiong,Dandan Qiu,Xiaoyan Xu,Tonghao Du,Zhen Peng,Muyan Xiao,Tao Huang,Jinqing Huang,Hongbing Ren,Mingyong Xie,Tao Xiong
Laotan Suancai is a well-known Chinese traditional fermented vegetable with a unique flavor. However, there are significant differences in the quality of Laotan Suancai pickled by jars (LTSC-J) and pools (LTSC-P) on an industrial scale. To reveal the effect of fermentation craft on Laotan Suancai quality, the microbial community, flavor metabolites, and physicochemical properties of the two types of Laotan Suancai, as well as their interrelationship were investigated. LTSC-P had higher salinity, glucose content, and histidine content, while LTSC-J had higher TA, lactic acid content, and isobutyric acid content. Nine differential aroma-active compounds were found between the two types of Laotan Suancai: anethole , eucalyptol, diallyl sulfide, estragole, diallyl disulfide , eugenol, linalool, nonanal , and ethyl butyrate. LTSC-P showed a higher relative abundance of Weissella , Acinetobacter , Pseudomonas , Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium , Inhella , and Leuconostoc compared to LTSC-J. Co-occurrence analysis indicated the distinct differences in the co-occurrence patterns of the microbial network across the two types of Laotan Suancai. RDA analysis indicated that glucose, salinity, TA, maltose , and nitrite were the main drivers for the differences in bacterial communities between the two types of Laotan Suancai. Spearman correlation analysis revealed strong correlations between 18 bacteria and 61 flavor compounds. Finally, bacterial metabolic function prediction indicated that sulfide and aromatic ester may be biosynthesized by sulfur metabolism , cysteine and methionine metabolism, pyruvate metabolism, and butanoate metabolism. Overall, these findings provide important guidance for improving the manufacturing and quality of Laotan Suancai.
研究领域:
食品科学
·
微生物学
·
发酵技术
·
风味化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2020
·
IF 0
Jianhong Yang,Zhiyong Song,Jun Ma,Heyou Han
With the rapid development of nanotechnology, molybdenum (Mo)-based nanomaterials have been widely used in various fields, and the ensuing environmental behavior has also raised widespread concern. Here, we report the nanotoxic effects and mechanism of nano-Mo treatment on the soybean–rhizobia symbiotic system in the sterilized mixture of sand and vermiculite environment. Exposure to different concentrations and types of Mo-based nanomaterials was shown to have significant physiological and biochemical toxicity to soybean. The accumulation of Mo in plant tissues increased with the concentration of nano-Mo in the sand and vermiculite media. The Mo-based nanomaterials that enter plant tissues were found to affect not only the growth and development of plants, but also the microstructure of the roots and the activity of rhizobium in the symbiotic system, thereby weakening the nitrogen fixation capacity of the soybean–rhizobia symbiotic system. Meanwhile, the activity of superoxide dismutase (SOD) and peroxidase (POD) showed a significant increase, indicating that these enzymes were activated after exposure to nano-Mo, which helps to remove the reactive oxygen species. Furthermore, the treatment of high-concentration Mo nanoparticles was shown to have obvious epigenetic toxicity to plants. These results demonstrate that high concentrations of Mo nanoparticles can affect the agronomical and physiological parameters in soybean, which may impact human nutrition and health.
研究领域:
植物学
·
环境科学
·
分子生物学
✓ 已验证
DOI
Nature Communications
·
2025
·Vol. 16
·期 1
·1-16
·
IF 15.7
Feng Qirui,Luo Yu,Liang Mu,Cao Yingui,Wang LingShuang,Liu Can,Zhang Xiaoyong,Ren Lanyang,Wang Yongfeng,Wang Daojie,Zhu Yantao,Zhang Yanfeng,Xiao Bo,Li Nannan
Endophytic plant growth promoting rhizobacteria (PGPRs) could replace chemical fertilizers in sustainable agriculture. Unfortunately, they are susceptible to harsh environmental conditions. Here, we proposed a polymeric hydrogel (PMH) consisting of carboxymethyl chitosan, sodium alginate, and calcium chloride for loading and protecting endophytic PGPR. This hydrogel can load endophytic PGPRs to not only boost its growth-promoting efficiency, but also help them adapt more effectively to environments. Using endophytic PGPR Ensifer C5 as model bacteria and Brasscia napus as host, we demonstrate that the PMH facilitate the colonization of endophytic PGPRs in the apical and lateral root primordia regions. Further analysis indicates that the PMH modulate suberin deposition of the endodermal cell layers and regulate the accumulation of auxin at the root tip. Meanwhile, PMH enhances the antioxidant capacity and disease resistance properties of plants by increasing the content of arachidonic acid metabolism intermediates in the plant. Importantly, the combination of PMH and endophytic PGPRs increases the yields of B. napus by approximately 30% in the field. Furthermore, PMH attenuates the loss of endophytic PGPR activity in the acidic environments. Overall, this microbial encapsulation strategy is a promising way to protect fragile endophytic microorganisms, providing attractive avenues in sustainable agriculture.
研究领域:
农业科学
·
微生物学
·
植物生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHYTOCHEMISTRY
·
2021
·
IF 4.072
Yu Jin,Yingting Xu,Zhengwan Huang,Zhongyu Zhou,Xiaoyi Wei
Casuarina equisetifolia L. (Casuarinaceae), an actinorhizal plant , exhibits mutualistic symbiosis with Frankia and promotes nitrogen fixation in root nodules . While the exchange of metabolites between host plant and microsymbiont is well understood in legume symbioses, the situation in the symbiosis between nitrogen-fixing Frankia and actinorhizal plants is less clear. In this study, a metabolomic approach was applied to root nodules of mature C. equisetifolia trees, leading to the identification of an undescribed taraxerane-type triterpenoid ester, 3- O -dihydrocoumaroyl β -taraxerol, along with twelve known compounds. An abundant component was tyramine with a content of 2.76 ± 0.315 mg/g FW in mature nodules. Tyramine specifically and abundantly accumulated in mature nitrogen-fixing nodules compared to senescent nodules, stems, leaves, and seeds. In addition, the potential function of tyramine was preliminarily examined and discussed.
研究领域:
植物生理学
·
共生关系
·
代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Science
·
2025
·
IF 14.1
Jing Ren,Qi Wang,Xiaxia Zhang,Yongheng Cao,JingXia Wu,Juan Tian,Yanjun Yu,Qingqiu Gong,Zhaosheng Kong
Legumes establish symbiosis with rhizobia by forming a symbiotic interface that enables cross-kingdom exchanges of signaling molecules and nutrients. However, how host organelles interact with symbiosomes at the symbiotic interface remains elusive during rhizobia endosymbiosis. Here, symbiotic cells are reconstructed using 3D scanning electron microscopy (SEM) and uncover that the host endoplasmic reticulum (ER) undergoes dynamic expansion to gradually enwrap symbiosomes, facilitating their compartmentalization and endosymbiosis. Consistently, altering ER lamellar expansion by overexpressing MtRTNLBs , the reticulons responsible for ER tubulation, impairs rhizobia accommodation and symbiosome development. Intriguingly, unfolded protein response (UPR)-marker genes, bZIP60 and IRE1A/B , show continuously activated expression during nodule development, and the two UPR-deficient mutants, ire1b , and bzip60 , exhibit compromised ER biogenesis and defective symbiosome development. Collectively, the findings underpin ER expansion and UPR activation as two key events in rhizobia accommodation and reveal an intrinsic coupling of ER morphology with proper UPR during root nodule symbiosis.
研究领域:
植物学
·
分子生物学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2024
·
IF 3.4
Puhong Yi,Mengdan Liu,Yuhua Hao,Ziwen Wang,Hanlin Liu,Xue Cai,Feng Cheng,Zhiqiang Liu,Yaping Xue,Liqun Jin,Yuguo Zheng
L-phosphinothricin (L-PPT) is the most popular broad-spectrum and highly effective herbicide. Transaminases (TAs) play a pivotal role in asymmetric synthesis of L-PPT, yet encounter the challenge of unfavorable reaction equilibrium. In this study, the novel dual transaminases cascade system (DTCS) was introduced to facilitate the synthesis of L-PPT. The specific amine transaminase BdATA, originating from Bradyrhizobium diazoefficiens ZJY088, was screened and identified. It exhibited remarkable activity, good stability, and required only 2.5 equivalents of isopropylamine to transform pyruvate effectively. By coupling BdATA with previously reported SeTA to construct the DTCS for pyruvate removal in situ , the L-PPT yield escalated from 37.37% to 85.35%. Three advantages of the DTCS were presented: the removal of pyruvate alleviated by-product inhibition, the use of isopropylamine reduced reliance on excess L-alanine, and no demand for expensive cofactors like NAD(P)H. It demonstrated an innovative idea for addressing the challenges associated with transaminase-mediated synthesis of L-PPT.
研究领域:
生物催化
·
酶工程
·
化学生物学
·
药物化学
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产品关联
DOI
PubMed
高影响因子
NANOTECHNOLOGY
·
2020
·Vol. 31
·期 44
·445501
·
IF 3.551
Wei Zhang,Xianghan Li,Qinyin Liu,Guanhui Liu,Guizhou Yue,Zhouping Yang,Yanying Wang,Hanbing Rao,Yinyin Chen,Changfang Lu,Xianxiang Wang
Fluorescent nitrogen-doped carbon dots (CDs) were prepared via hydrothermal method at 190 °C for 10 h using rhizobium from soy as the carbon and nitrogen source. Their optical properties, structure, morphology, and functional groups were characterized in detail and the results showed that they possess unique excitation-dependent fluorescence behavior, with average diameter 4.5 ± 2.0 nm and good water dispersibility. Due to the overlap of the UV–vis absorbance of chlortetracycline hydrochloride (CCH) and the fluorescence excitation band of CDs, the fluorescence of the prepared CDs can be quenched by CCH selectively and sensitively. The changes of the fluorescence intensity of CDs have a good linear relationship with the concentration of CCH in a wide concentration range of 5–100 μM, with a detection limit of 0.254 μM. This present method has been successfully applied to determine the CCH in water with recovery ranging from 96.0% to 100.7%.
研究领域:
材料科学
·
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2021
·
IF 6.291
Zhijian Ke,Qian Zhu,Wankui Jiang,Yidong Zhou,Mingliang Zhang,Mingli Jiang,Qing Hong
Carbaryl is the representative of carbamate insecticide. As an acetylcholinesterase inhibitor, it poses potential threat to humans and other non-target organisms. Agrobacterium sp. XWY-2, which could grow with carbaryl as the sole carbon source, was isolated and characterized. The carH gene, encoding a carbaryl hydrolase, was cloned from strain XWY-2 and expressed in Escherichia coli BL21 (DE3). CarH was able to hydrolyze carbamate pesticides including carbaryl, carbofuran, isoprocarb, propoxur and fenobucarb efficiently, while it hydrolyzed oxamyl and aldicarb poorly. The optimal pH of CarH was 8.0 and the optimal temperature was 30 ℃. The apparent K m and k cat values of CarH for carbaryl were 38.01 ± 2.81 μM and 0.33 ± 0.01 s −1 , respectively. The point mutation experiment demonstrated that His341, His343, His346, His416 and D437 are the key sites for CarH to hydrolyze carbaryl.
研究领域:
环境科学
·
微生物学
·
生物化学
·
酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Ruiping Song,Yuanze Sun,Xinfei Li,Changfeng Ding,Yi Huang,Xinyu Du,Jie Wang
Concerns about the ecological safety of both conventional and biodegradable microplastics have grown due to the inadequate end-of-life treatments of plastics. In this study, the effects of conventional and biodegradable microplastics on the spread of antibiotic resistance genes (ARGs) and virulence factors (VFs) were estimated in a soil microcosm experiment. The gene profiles and their respective bacterial hosts in soil were evaluated by metagenomic sequencing methods. The abundances of ARGs and VFs in polybutylene succinate (PBS) treated soils were statistically higher than the values in the control and conventional microplastic treatments. In comparison with the control, application of conventional microplastics showed negligible effects on ARG and VF profiles in the soil, while biodegradable microplastic amendments significantly changed the compositions of ARGs and VFs. The host-tracking analysis suggested application of microplastics broadened the bacterial hosts of ARGs and VFs in the soil. The percentage of Proteobacteria as ARG hosts increased from 38.5% in the control soils to 58.2% in microplastic exposed soil. The genus Bradyrhizobium was the dominant host of ARGs and VFs in biodegradable microplastic treatments, while conventional microplastics increased the percentages of Pseudomonas as the bacterial hosts. This study enhances the understanding of the effects of conventional and biodegradable microplastics on the propagation and hosts of ARGs and VFs in the terrestrial environment, providing essential insights into the risk assessment and management of plastics.
研究领域:
环境科学
·
微生物学
·
基因组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Yidong Zhou,Zhijian Ke,Hangting Ye,Mengting Hong,Yifei Xu,Mingliang Zhang,Wankui Jiang,Qing Hong
Carbaryl is a widely used carbamate pesticide in agriculture. The strain Rhizobium sp. X9 possesses the typical carbaryl degradation pathway in which carbaryl is mineralized via 1-naphthol, salicylate, and gentisate. In this study, we cloned a carbaryl hydrolase gene cehA and a novel two-component 1-naphthol hydroxylase gene cehC1C2. CehA mediates carbaryl hydrolysis to 1-naphthol and CehC1, an FMNH2 or FADH2-dependent monooxygenase belonging to the HpaB superfamily, and hydroxylates 1-naphthol in the presence of reduced nicotinamide-adenine dinucleotide (FMN)/flavin adenine dinucleotide (FAD), and the reductase CehC2. CehC1 has the highest amino acid similarity (58%) with the oxygenase component of a two-component 4-nitrophenol 2-monooxygenase, while CehC2 has the highest amino acid similarity (46%) with its reductase component. CehC1C2 could utilize both FAD and FMN as the cofactor during the hydroxylation, although higher catalytic activity was observed with FAD as the cofactor. The optimal molar ratio of CehC1 to CehC2 was 2:1. The Km and Kcat/Km values of CehC1 for 1-naphthol were 74.71 ± 16.07 μM and (8.29 ± 2.44) × 10–4 s–1·μM–1, respectively. Moreover, the enzyme activities and substrate spectrum between CehC1C2 and previously reported 1-naphthol hydroxylase McbC were compared. The results suggested that McbC had a higher 1-naphthol hydroxylation activity, while CehC1C2 had a broader substrate spectrum.
研究领域:
环境微生物学
·
生物化学
·
农药生物降解
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Plants-Basel
·
2024
·Vol. 13
·期 2
·317
·
IF 4
Xiu Wang,Yaqi Liu,Na Peng,Haitao Yu,Yu Ma,Mingxin Zhang,Yaoyao Wang,Yi Wang,Weiwei Gao
The volatile compounds produced by plants play an important role in plant growth, plant communication, and resistance to biological and abiotic stresses.Astragalus membranaceusvar.mongholicus(AM) is a perennial herbaceous plant (Leguminosae) that is widely cultivated in northwest China. The bioactive compounds in its root have shown various pharmacological activities. Root rot disease caused byFusariumspp. often occurs inAMplanting with increasing severity in continuous monoculture. It is currently still unclear what are the effects of the volatile compounds produced by freshAMon itself, other crops cultivated on the same field afterAM, pathogen, and rhizobia. In this study, we found that seed germination and seedling growth ofAM, lettuce (Lactuca sativaL.), and wheat (Triticum aestivumL.) could be affected if they were in an enclosed space with freshAMtissue. Additionally, 90 volatile compounds were identified by SPME-GC-MS from wholeAMplant during the vegetative growth, 36 of which were specific to aerial parts ofAM(stems and leaves, AMA), 17 to roots (AMR), and 37 were found in both AMA and AMR. To further identify the allelopathic effects of these volatile compounds, five compounds (1-hexanol, (E)-2-hexenal, (E,E)-2,4-decadienal, hexanal, and eugenol) with relatively high content inAMwere tested on three receptor plants and two microorganisms. We found that (E,E)-2,4-decadienal and (E)-2-hexenal showed significant inhibitory effects on the growth ofAMand lettuce. One-hexanol and hexanal suppressed the growth of wheat, while eugenol showed a similar effect on all three plant species. Moreover, the activities of these compounds were dose dependent. Notably, we discovered that (E)-2-hexenal and eugenol also inhibited the growth of the pathogenFusarium solaniby as high as 100%. Meanwhile, all five compounds tested suppressed the rhizobiaSinorhizobium fredii. In summary, this study furthered our understanding of the comprehensive allelopathic effects of the main volatile components ofAM.
研究领域:
植物科学
·
药理学
·
微生物学
·
农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）