Foods
·
2024
·Vol. 13
·期 13
·2010
·
IF 4.7
Yan Chen,Jing Liu,Yifan Xu,Chaoqun Sun,Wenjie Qu,Hanchen Du,Menglu He,Junsheng Huo,Jing Sun,Jian Huang,Jiyong Yin
The main active component ofPolygonatum sibiricum(P. sibiricum) rhizome isPolygonatum sibiricumPolysaccharide (PsP) with antioxidant function. At present, only the mature rhizome ofP. sibiricumis used to extract PsP, while the young rhizome of by-product is discarded directly as waste, resulting in significant wastage ofP. sibiricumresources. We used ultrasound-assisted extraction-deep eutectic solvents (UAE-DESs) method to extract PsP of young and mature rhizomes, respectively. The extraction rate, structure composition and antioxidant ability of PsP between young and mature rhizomes were compared, so as to provide references for comprehensive utilization ofP. sibiricumresources. The PsP extraction rate (33.88 ± 1.95%) of young rhizome was close to that (45.08 ± 1.92%) of mature rhizomes. The main component (PsP-2) of the PsP in young rhizome contained six kinds of monosaccharides, which belonged to acidic polysaccharides. The above characteristics of the PsP of young rhizome were similar to those of mature rhizome. The PsP of young rhizome also exhibited similar biological activity to that of the mature rhizome, which indicated even more advantages in DPPH free radical scavenging ability. The results of this study support the utility of the young rhizome, consequently helping to avoid unnecessary waste and provide reference for comprehensive utilization ofP. sibiricum.
研究领域:
药用植物学
·
植物化学
·
天然产物研究
·
生物活性
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOCHEMICAL SYSTEMATICS AND ECOLOGY
·
2025
·
IF 2
Lue Hong,Zerong Wu,Jiahui Gong,Jiahui Ma,Shengzhi Liu
Alocasia cucullata (Lour.) Schott is a member of the Araceae family and is characterized by its unique leaf morphology and vibrant flowers; thus, it is a valuable addition to gardens and interior decor. However, A. cucullata may contain toxic compounds, including calcium oxalate crystals, lectins, and alkaloids, and their distribution within the plant tissues remains poorly understood. In this study, untargeted metabolomics was used to analyse the distribution of the bioactive compounds across the roots, stems, leaves, and guttation of A. cucullata . The toxic effects of these tissues on the MDA-MB-231 human breast cancer cell line were evaluated using the MTT assay. Microscopic techniques were utilized to examine the microstructure of the guttation and stems, and the focus was on the morphology of the calcium oxalate crystals. Liquid chromatography and ELISA kits were used to quantify the calcium oxalate and lectin contents in the various tissues. The results demonstrated that the active constituents, primarily fatty acids, phenylpropanoids, flavonoids, peptides, and alkaloids, were concentrated in the roots. Notably, guttation exhibited no toxicity and lacked both calcium oxalate crystals and significant lectin levels. This research enhances the understanding of the bioactive composition and safety profile of A. cucullata and provides a theoretical foundation for future scientific investigations and safe cultivation, with implications for both scientific and industrial applications.
研究领域:
植物学
·
毒理学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
·
2014
·
IF 0.638
Zhisheng Xie,Xinjun Xu,Chunyan Xie,Zhikun Liang,Mei Yang,Jieyun Huang,Depo Yang
Tetrandrine and fangchinoline were successfully separated by reversed-phase flash chromatography on a manually-packed C18 column from Radix Stephania tetrandra. After recrystallization by acetone, 13 mg of fangchinoline and 21 mg of tetrandrine were obtained from 100 mg Stephania tetrandra extract, with their relative content 98.78% and 98.19%, respectively. Their structures were characterized by UV, IR, MS, and NMR. The established method was simple, fast, and reproducible, which can be applied to the preparation of reference substances of tetrandrine and fangchinoline.
研究领域:
植物化学
·
药物化学
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Chentao Huang,Jiabei Xia,Jiayun Xu,Xingyu Mei,Huan Cheng,Shiguo Chen,Jinping Si,Xingqian Ye,Jianle Chen
Polygonatum a widely recognized medicinal and edible plant, exhibits several confirmed biological activities and holds significant market potential. Numerous studies have identified polysaccharides are one of the primary bioactive components in the rhizomes of Polygonatum . Our previous research identified a notable presence of fructo-oligosaccharides in the rhizomes of Polygonatum sibiricum , highlighting its largely overlooked potential as a natural source of bioactive compounds. Therefore, a more systematic and comprehensive analysis of water-soluble carbohydrates across different species and plant parts of Polygonatum is essential. The aim of this study was to investigate the carbohydrate composition in four different Polygonatum species, focusing on the rhizomes, roots, leaves, stems, and fruits. In particular, we utilized a self-established method for the determination of fructo-oligosaccharides. The results indicated the presence of small amounts of mono/disaccharides and polysaccharides, as well as a considerable quantity of fructo-oligosaccharides in rhizome, root, fruit of Polygonatum . The low-molecular-weight glycans was the predominant carbohydrate in the rhizomes of Polygonatum Mill. spp., comprising over 80% of the total soluble carbohydrates, with polysaccharides contributing approximately 11%, and mono/disaccharides less than 10%. Our study offers valuable insights and a meaningful basis for the excavation of the functional carbohydrate resources of the Polygonatum Mill.
研究领域:
植物化学
·
药用植物学
·
功能食品科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF WOOD CHEMISTRY AND TECHNOLOGY
·
2023
·
IF 2
Yinping Li,Qing He,Xiaoqing Zhu,Zhufeng Geng,Yi Wang,Jin Li
Daphne altaica Pall. is an important plant in traditional Chinese medicine. Mineral elements play an important role in the formation of active ingredients in medicinal plants. In this study, 14 mineral elements were quantitatively determined by inductively coupled plasma emission spectrometry using the roots, stems and leaves of D. altaica. The distribution variance of mineral elements between different organs were analyzed by statistical analysis methods such as single factor analysis of variance (ANOVA), correlation analysis (CA), principal component analysis (PCA) and cluster heat map. The results show that the mineral element contents in corresponding parts of D. altaica exhibited significant variation, with the order from highest to lowest being K > Ca > Mg > Fe > Al > Mn > Na > Zn > Sr > Cr > Ni > Cu > Pb > Mo. Among them, the highest content of mineral elements are K, Ca, Mg, Mn, Cu and Mo in leaves, and the stems contains more Zn, Sr, Cr and Ni, while the content of mineral elements Fe, Al, Na and Pb in roots is much higher than the other two organs. The ANOVA revealed significant variations in the mineral element content across different regions of D. altaica (p < 0.05). Based on single factor analysis of variance, different parts of D. altaica had different fingerprint of mineral elements, and the concentration of the same mineral elements in different parts had significant difference (p < 0.05). The principal component analysis (PCA) model showed that the cumulative variance contribution rate of first two principal components was 99.10%, in which Mn, Mg and Ca have higher load values, which is consistent with the results of bi-plot and cluster analysis. These results indicated that there were great differences in absorption and utilization of mineral nutrient elements between roots, stems and leaves of D. altaica, and there was significant correlation among mineral elements, indicating that there was a complex synergistic and antagonistic effect among the elements. At the same time, the discriminant analysis of the characteristic elements provided a scientific basis for the quality control of D. altaica.
研究领域:
植物学
·
药用植物研究
·
化学分析
✓ 已验证
DOI
高影响因子
Metabolites
·
2024
·Vol. 14
·期 8
·409
·
IF 3.4
Nana Chang,Xianping Yang,Xiaoqing Wang,Chao Chen,Chu Wang,Yang Xu,Hengyu Huang,Ye Wang
Drynaria roosii Nakaike, a fern widely distributed in China and some countries in Southeast Asia, is a commonly used herbal medicine in tonic diets and Chinese patented medicine. The metabolites of its dried rhizomes are easily affected by the epiphytic pattern, whether on rock tunnels (RTs) or tree trunks (TTs). The current research focused on rhizomes from these two patterns, RTs and TTs (further divided into subclasses TA, TB, TC, and TD, based on trunk differences) and conducted a widely targeted metabolomics analysis. A total of 1435 components were identified across 13 categories, with flavonoids, amino acids, and their derivative, lipids, identified as the main components. They accounted for 19.96%, 12.07%, and 12.14% of all metabolites, respectively. The top five flavonoids in TB were eriodicty-ol-7- O -(6″-acetyl)glucoside, quercetin-3- O -sophoroside (baimaside), dihydrochar-cone-4′- O -glucoside, morin, and hesperetin-7- O -glucoside, with relative contents 76.10, 24.20, 17.02, 15.84, and 14.64 times higher than in RTs. Principal component analysis revealed that samples with different epiphytic patterns clustered into five groups. The RT patterns revealed unique metabolites that were not detected in the other four epiphytic species (TA, TB, TC, and TD), including 16 authenticated metabolites: 1 alkaloid, 1 amino acid derivative, 7 flavonoids, 2 lignans, 1 lipid, 1 alcohol, 1 aldehyde, and 2 phenolic acids. These differences in epiphytic patterns considerably affected the accumulation of both primary and secondary metabolites. The comparison of diversity between RTs and TTs can guide the selection of a cultivation substance and the grading of collective rhizomes in the wild. This comprehensive analysis of D. roosii rhizome metabolites also offers fundamental insights for identifying active components and understanding the mechanisms underlying their potential pharmacological activities.
研究领域:
药理学
·
植物学
·
代谢组学
·
中医药
✓ 已验证
产品关联
DOI
PubMed
高影响因子
HORTSCIENCE
·
2021
·Vol. 56
·期 8
·909-914
·
IF 1.455
Asma Mohammed Saeed Al-Kubati,Baoshan Kang,Liming Liu,Aqleem Abbas,Qinsheng Gu
Zucchini yellow mosaic virus (ZYMV) causes serious damage to cucurbit crops worldwide and can be spread by aphids, by mechanical injury, and in seeds. With the popularization of cucurbit grafting, the use of susceptible rootstock has increased the risk of ZYMV infection in cucurbit crops. In China, the bottle gourd (Lagenaria siceraria) is a widely used rootstock in grafted watermelon production. However, few resistant bottle gourds are available commercially. This study developed bottle gourd lines resistant to ZYMV using ethyl methanesulfonate (EMS) mutagenesis. A new mutated bottle gourd population (M1) was generated by treating seeds with EMS. Diverse phenotypes were observed in the seedlings, flowers, and fruit of M2 plants, some of which are of potential commercial interest, such as dwarfing and different fruit shapes. Based on the M2 phenotypes, 106 M3 lines were selected and screened for resistance to ZYMV by mechanical inoculation and agroinfiltration. Nine M3 lines were resistant to ZYMV during three tests. One inbred M4 line (177-8) was developed and showed stable resistance and no virus when tested using a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and polymerase chain reaction. These resistant lines are promising materials for developing watermelon rootstock and exploring resistance genes as new ZYMV-resistant resources. EMS induction could be a practical strategy for creating resistant cucurbit crops.
研究领域:
植物学
·
遗传学
·
作物科学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2015
·
IF 2.858
Jie Hu,Xuejing Jia,Xiaobin Fang,Peng Li,Chengwei He,Meiwan Chen
Ultrasonic-assisted extraction technology was employed to prepare Ligusticum chuanxiong Hort polysaccharide. Single factor test and orthogonal experimental design were used to optimize the extraction conditions. The results showed that the optimal extraction conditions consisted of ultrasonic temperature of 80 °C, ultrasonic time of 40 min and water to raw material ratio of 30 mL/g. Three novel polysaccharides fractions, LCX0, LCX1 and LCX2, were isolated and purified from the crude polysaccharides using DEAE-52 cellulose and Sephadex G-100 column chromatography. The molecular weight and monosaccharide composition of three LCX polysaccharides fractions were analyzed with gel permeation chromatography (GPC) and HPLC analysis, respectively. Furthermore, the antioxidant and in vitro anticancer activities of the polysaccharides were investigated. Compared with LCX0, LCX2 and LCX1 showed relative higher antioxidant activity and inhibitory activity to the growth of HepG2, SMMC7721, A549 and HCT-116 cells. It is suggested that the novel polysaccharides from rhizome of L. chuanxiong could be promising bioactive macromolecules for biomedical use.
研究领域:
药理学
·
生物化学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACTA PHYSIOLOGIAE PLANTARUM
·
2024
·Vol. 46
·期 3
·1-11
·
IF 2.4
Wu Wenqing,Li Simeng,Gu Wei,Tian Rong,Luo Man,Tang Junjie,Tang Min,Wu Yinzhi
Alismatis rhizoma is an essential medicine in clinical practice. Asian water plantain ( Alisma orientale (Sam.) Juzep) is one of the original plants of Alismatis rhizoma. Previous studies have identified the optimal light intensity range for the growth and development of A. orientale , but the mechanism by which light intensity affects the accumulation of secondary metabolites of A. orientale is unknown. The aim of this study was to investigate the effect of light intensity on the accumulation of triterpenoids in A. orientale saplings and its potential molecular mechanism. The dry weight and contents of total triterpenes and indicative components (alisol B 23-acetate and alisol C 23-acetate) as well as the expression of key enzyme genes in the triterpene biosynthesis pathway under different light intensities (50–600 μmol m −2 ·s −1 ) were determined. The results showed that the accumulation of dry matter and the contents of total triterpenes, alisol B 23-acetate, and alisol C 23-acetate increase first and then decrease with increasing light intensity, with the maximum values of 31.65 g, 18.35 mg·g −1 , 1.91 mg·g −1 , and 0.13 mg·g −1 recorded at light intensities of 400, 200, and both of 300 μmol m −2 ·s −1 , respectively. Light intensities of 200–400 μmol m −2 ·s −1 promote the expressions of key enzyme genes and the accumulation of total triterpenes significantly. Correlation analysis showed that the expression levels of key enzyme genes are significantly correlated with total triterpene and indicative component contents, and these correlations are strongest under moderate light intensities. Overall, our results reveal that a moderate light intensity of 200–400 μmol m −2 ·s −1 is beneficial for the growth and synthesis of protostane triterpenes in A. orientale seedlings, and that its probable mechanism involves the upregulated expression of enzymes that are key in the synthesis of triterpenoid ingredients. This study clarified the suitable light intensity range for the synthesis and accumulation of protostane triterpenes of A. orientale , which provided scientific basis for the production of high-quality superior forms of A. orientale .
研究领域:
生药学
·
植物生理学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
BIOMEDICAL CHROMATOGRAPHY
·
2014
·Vol. 29
·期 6
·860-868
·
IF 1.662
Xiaoping Zhang,Huiqing Lv,Zuguang Li,Kezhi Jiang,Maw-Rong Lee
Ficus pandurata H. aerial roots are used as a traditional Chinese medicine for the treatment of uarthritis, indigestion and hyperuricemia. However, the bioactive constituents responsible for the pharmacological effects of F . pandurata H. are unclear. A simple and efficient HPLC/QTOF-MS/MS (high-performance liquid chromatography/electrospray ionization with quadrupole time-of-flight tandem mass spectrometry) method was established to detect and identify active constituents in the n -butanol extract of F . pandurata H. aerial roots. Chemical constituents were separated and investigated by HPLC/QTOF-MS/MS in the negative-ion mode. Thirty-seven compounds, including hydroxycinnamic acid derivatives, hydroxybenzoic acid derivatives, hydroquinone glycosides, flavonoid glycosides, etc., were identified or tentatively characterized in the n -butanol extract of F . pandurata H. aerial roots by comparing the UV spectra, accurate mass spectra and fragmentation pathways and retrieving the reference literatures. Moreover, the flavonoid trisaccharides and hydroxybenzoic acid derivatives were tentatively characterized in F . pandurata H. for the first time. The analytical tool used here is very valuable in the rapid separation and identification of the multiple and minor constituents in the n -butanol extract of F . pandurata H. aerial roots. Copyright © 2014 John Wiley & Sons, Ltd.
研究领域:
药理学
·
分析化学
·
植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2020
·
IF 3.004
Dandan Wang,Xinxin Luo,Min Wang,Kai Zhou,Zhining Xia
Molecularly imprinted polymers (MIPs) based on polydatin were prepared by precipitation polymerization method. Synthesis process of MIPs was optimized by discussion of functional monomers, porogens and the molar ratio of template- functional monomer-cross linker. Then, MIPs were prepared with polydatin as the template, 4-vinyl pyridine as the functional monomer, ethylene glycol dimethyl acrylate as the cross linker, acetonitrile as the porogen and the molar ratio of template-monomer-cross linker at 1:10:20. Scanning electron microscopy and Fourier transform infrared spectrometer were used to inspect macroscale and chemical bond of MIPs. Adsorption capability and selectivity of MIPs to polydatin were investigated by carrying out the static, dynamic and selective experiments. The results showed MIPs performed high adsorption ability and selectivity to polydatin, indicating MIPs could be used to separate and enrich polydatin from the complex systems. Finally, MIPs were applied as the adsorbent for isolation and purification of polydatin from the extract of Polygoni Cuspidati Rhizoma et Radix , rats' plasma and urine samples. MIPs were successfully used to separate polydatin from the Polygoni Cuspidati Rhizoma et Radix and recovery ranged from 89.2% to 91.6%. The maximum concentration of polydatin in rats' plasma and urine samples was 2.84 ± 0.0748 µg mL −1 and 2.64 ± 0.485 µg mL −1 , respectively. Moreover, to compare with the MIPs method, organic solvent methods were used to analyze the polydatin in rats' plasma and urine samples. The results illustrated MIPs method was effective and selective for enrichment of polydatin from the medicinal plants and biological samples.
研究领域:
药物化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Yujuan Cai,Jun Zhang,Yulian Wen,Zefan Zhang,Hailong Wang,Ye Yang,Zhigang Tai
As global food, it has important implications for monitoring and testing of pesticide pollution in the root and tuber crops. Herein, a novel mixed-matrix membrane based on metal–organic frameworks (MOF-525-MMM) was successfully prepared for a simplified dispersive solid-phase extraction (d-SPE) of ten kinds of pesticides in root and tuber crops. The MOF-525-MMM was well characterized and major factors affecting extraction process were systematically investigated. Under optimal conditions, the MOF-525-MMM based d-SPE coupled with GC–MS exhibited excellent linearity (R 2 ≥ 0.9947) and detection limits of 0.80–4.16 µg/L. Finally, the developed method was successfully applied to the determination of pesticides in potatoes and sweet potatoes, with recoveries in range of 65.3–92.5 % and RSDs lower than 9.8 %. In the extraction process, MOF-525-MMM exhibited excellent extraction efficiency and purified ratio. Therefore, the adsorption mechanism was studied, and the results indicated that it was attributed to the π-π stacking effect and hydrogen bond between MOF-525 and pesticides. It was demonstrated that this d-SPE-GC–MS method based on MOF-525-MMM offers great potential for efficient extraction and determination of pesticides in root and tuber crops.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）