ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
·
2018
·Vol. 98
·期 1
·e21452
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IF 1.316
Jing Zhang,Deye Luo,Peng Wu,Haozhe Li,Hongyu Zhang,Weiwei Zheng
Odorant-binding proteins (OBPs) in insects are essential for mating and oviposition host selection. How these OBPs respond to different hosts at the mRNA level and their effects on behavior remain poorly characterized. The oriental fruit fly Bactrocera dorsalis is a highly invasive agricultural pest with an extremely broad host range and high fecundity. Based on our previously constructed B. dorsalis transcriptome, six OBP s that were differentially expressed during three different physiological adult stages were identified. A phylogenetic tree was constructed to illustrate the relationships of these six OBP s with OBP sequences from three other dipteran species ( Drosophila melanogaster , Anopheles gambiae , and Ceratitis capitata ). The spatiotemporal expression profiles of the six OBP s were analyzed using quantitative real-time PCR. Our results revealed that OBP19c, OBP44a, OBP99a , and OBP99d were abundantly expressed from the prepupa stage to the adult stage, and most of the OBP s were mainly expressed in the head, wings, and antennae. The expression levels of these OBP s were upregulated when female flies were exposed to their preferred hosts. Silencing OBP99a resulted fewer eggs being laid compared with the control group when the females were exposed to their preferred host, that is, banana, whereas more eggs were laid when a non-preferred host, that is, tomato, was used. Furthermore, silencing OBP99a led to sexually dimorphic mating behavior. dsOBP99a -injected males dramatically reduced courtship, whereas enhanced courtship was observed in the treated females. These data indicate that OBP s may participate in different biological processes of B. dorsalis . Our study will provide insight into the molecular mechanism of chemoreception and help develop ecologically friendly pest-control strategies.
研究领域:
昆虫学
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分子生物学
·
农业害虫防治
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
·
2016
·
IF 0.824
Lu Liu,Qiong Zhou
Citrus fruit fly Bactrocera minax (Enderlein) (Diptera:Tephritidae) is a devastating pest of citrus plants in China. In order to characterize the behavioral mechanisms and possible chemical cues involved in host selection by female citrus fruit fly, the host plant volatiles was analyzed and Y-tube olfactometer assays were used. Host odor from citrus fruits peels elicited upwind orientation flights followed by landing and egg-laying, demonstrating the essential role of host plant volatiles in B. minax host-finding and oviposition behavior. GC–MS analysis of volatiles from the peels of three varieties citrus fruits, that were Bingtang sweet oranges ( Citrus sinensis cv. Bingtang), Satsumas mandarins ( Citrus reticulata cv. Satsuma) and Sour oranges ( Citrus aurantium ), which adult and larval of B. minax preferred to, showed that the major compounds extracted from the basal region were different from those of the distal region to some extent. Furthermore, in Y-tube olfactometer attraction assays, female flies were significantly attracted ( P < 0.05) to four out of the thirteen tested compounds from the citrus volatiles, including nonanal , citral , limonene and linalool . Conversely, furfuryl alcohol and guaiastil significantly repelled females ( P < 0.05). The percentage composition of nonanal, citral, limonene and linalool from different regions of the fruits displayed a positive correlation with the numbers of egg marks made by females, whereas furfuryl alcohol and guaiastil displayed a correspondingly negative correlation. These results suggest that the oviposition preference of B. minax females is highly related to the chemical compounds emitted from host fruit peels.
研究领域:
昆虫学
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植物化学
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化学生态学
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产品关联
DOI
高影响因子
POLYMER
·
2023
·
IF 4.6
Yinjie Qian,Junjie Zhang,Yanxue Yu,Qiushi Jiang,Bin Yan,Xinlong Song,Xiaobin Yu,Zhiqiang Cheng
Methyl eugenol (ME) is a highly potent sexual attractant for Bactrocera dorsalis , which plays a crucial role in agricultural pest management. In this study, a composite fiber membrane was prepared by polystyrene (PS), polyethylene glycol diacrylate (PEGDA), and ME through electrostatic spinning respectively. The effective release period of ME was prolonged by triggering PEGDA with UV light. The mass ratio of PS to PEGDA affects the morphology of the fibers as well as the loading capacity (LC) and encapsulation efficiency (EE) for ME. Under optimal test conditions, the diameter of the fibers was 1.70 μm, and the ME loading capacity and encapsulation efficiency were (7.80 ± 0.07%) and (85.84 ± 7.94%). The combination of PS and PEGDA was confirmed by FTIR analysis, while its excellent thermal stability was verified through TGA testing. In the PS/PEGDA-ME-UV membrane, the cumulative release rate of ME reached 81.97% by week 8, which conformed to a first-order model. In the trapping experiments, a 53% rate of Bactrocera dorsalis capture was maintained even by week 8, demonstrating its potential for application in agricultural biocontrol.
研究领域:
农业科学
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昆虫学
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材料科学
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DOI
高影响因子
Scientific Reports
·
2020
·Vol. 10
·期 1
·1-11
·
IF 3.998
Zhou You,Qin De Qiang,Zhang Pei Wen,Chen Xiao Tian,Liu Ben Ju,Cheng Dong Mei,Zhang Zhi Xiang
Azadirachtin exhibits excellent bioactivities against several hundred arthropods. However, current knowlege of its biochemical effect on B . dorsalis larvae is not deep enough. In this study, integrated LC-MS and GC-MS-based untargeted metabolomics were used to analyze the changes of endogenous metabolites and the biochemical effects of azadirachtin on B . dorsalis larvae. Azadirachtin has excellent bioactivities against B . dorsalis larvae in this study, leading to a longer developmental duration, lower survival rate, and low pupa weight. The effect of azadirachtin was investigated on a total of 22 and 13 differentially abundant metabolites in the LC–MS and GC–MS-based metabolomics results, are selected respectively. Pathway analysis indicated that 14 differentially enriched metabolic pathways, including seven influential pathways, are worthy of attention. Further integrated key metabolic pathway analysis showed that histidine metabolism, d -glutamine and d -glutamate metabolism, biotin metabolism, ascorbate and aldarate metabolism, pentose and glucuronate interconversions, and alanine, aspartate and glutamate metabolism in B . dorsalis larvae are significantly relevant pathways affected by azadirachtin. Although extrapolating the bioactivity results in this study to the practical project of B . dorsalis pest management in the field has limitations, it was found that azadirachtin has a significant effect on the primary metabolism of B . dorsalis larvae.
研究领域:
昆虫学
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代谢组学
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植物化学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
·
IF 8.025
Zhao Liu,Qian-Ping Xie,Huai-Wang Guo,Wei Xu,Jin-Jun Wang
Odorant-binding proteins (OBPs) are believed to play critical roles in host-seeking behavior. However, little attention was paid to its different functions in male and female. The antenna-specific OBP gene from Bactrocera dorsalis , BdorOBP13, was cloned and its expression profile was examined. The results showed that BdorOBP13 was exclusively expressed in male and female adults, which exhibited a high transcript level in antennae. After injection of BdorOBP13 dsRNA, its transcript level in males and females decreased significantly. Electrophysiological responses of RNAi-injected flies to, methyl eugenol (male attractant) and γ-octalactone (female attractant) decreased significantly. However, no significant changes in the electrophysiological response were observed in RNAi-injected flies to benzothiazole, (+),dipentene, and ethyl tiglate. The behavioral bioassay showed that males treated with RNAi significantly reduced their preference to methyl eugenol, while RNAi-injected females showed a significantly lower preference to γ-octalactone, suggesting that BdorOBP13 may have different functions between males and females: it may be involved in the detection of methyl eugenol in males but is involved in the detection of γ-octalactone in females. These findings improve our understanding of insect OBPs and their roles in insect chemosensation, which may provide us with new molecular targets in the management of B. dorsalis .
研究领域:
昆虫学
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分子生物学
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害虫管理
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2022
·
IF 9.231
Tao Wen,Qiyi Nie,Longbo Han,Zhongliang Gong,Dapeng Li,Qiang Ma,Zhiyu Wang,Weitao He,Liang Wen,Hailong Peng
D- limonene is the main volatile gas in citrus, and its content changes significantly after the citrus becomes infected, thus making it possible to use gas-derived information to identify pest-infested citrus. In this study, a quartz crystal microbalance (QCM) sensor based on a molecularly imprinted polymers (MIPs) coating was used to identify the citrus infested with Bactrocera dorsalis . The experimental results show that within the concentration range of 300–2100 mg m −3 , the sensor’s response to the concentration follows a linear trend, and its sensitivity was 0.078 Hz (mg m −3 ) −1 ; the detection limit was 41.40 mg m −3 . The sensor’s reproducibility and repeatability were both higher than 95%, and the one-month frequency drop deviation was less than 5%. The results confirmed that the MIPs-QCM sensor can rapid detection and nondestructive of infested citrus.
研究领域:
分析化学
·
植物保护
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DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2019
·
IF 4.527
You Zhou,De-Qiang Qin,Pei-Wen Zhang,Ben-Ju Liu,Xiao-Tian Chen,Zhi-Xiang Zhang
Azadirachtin , as the most promising and effective botanical insecticide, exhibits significant growth inhibition activity against agricultural and forestry pests. However, its biochemical effects at the metabolic level compared with those of other insect growth regulators have not been studied. Therefore, in this study, a GC–MS based untargeted metabolomics approach was applied to compare azadirachtin with pyriproxyfen (a juvenile hormone analog) and tebufenozide (a molting hormone analog) in terms of their metabolic effects on Bactrocera dorsalis larvae. The bioactivity of azadirachtin against B. dorsalis larvae was significantly different than those of pyriproxyfen and tebufenozide. A total of 693 mass features were recognized, and 112 metabolites were identified in this study. The results showed that a total of 16, 13 and 10 differentially regulated metabolites corresponding to 12, 5 and 8 pathways occur in Aza versus CK, Pyr versus CK and Teb versus CK group, respectively. Further analysis showed that 6 differentially regulated metabolites corresponding to 5 key pathways could be the primary differential metabolic response of B. dorsalis larvae to the three insect growth regulators. The pathways were myo-inositol corresponding to ascorbate and aldarate metabolism as the specific response of B. dorsalis larvae to azadirachtin; xylitol, xylulose and 3-aminopropionitrile corresponding to pentose and glucuronate interconversions, and cyanoamino acid metabolism as the common responses to azadirachtin and pyriproxyfen; and 3-hydroxypropionic acid and beta-alanine corresponding to propanoate metabolism and beta-alanine metabolism as the specific responses to tebufenozide. The results showed that the metabolic response of B. dorsalis larvae to azadirachitin is closer to that of pyriproxyfen than tebufenozide. The differentially regulated metabolites and pathways responsible for this difference are discussed.
研究领域:
昆虫学
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植物保护
·
代谢组学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Tao Wen,Weitao He,Qiyi Nie,Zhongliang Gong,Dapeng Li,Liang Wen,Hailong Peng
The characteristic substances in citrus volatile organic compounds (VOCs) are associated with the infestation of the Bactrocera dorsalis (Hendel), which provides a noninvasive evaluation method to discriminate infested citrus. This paper developed an olfactory detection system based on a quartz crystal microbalance (QCM) sensor array and classification algorithms to identify the citrus infested with B. dorsalis . Six characteristic substances, including d -limonene, myrcene, α-pinene, decanal , linalool , and β-ocimene, which vary significantly after B. dorsalis infestation were selected as template molecules to prepare molecularly imprinted polymers (MIPs) and modify QCM respectively. The experimental results show that the prepared MIPs-QCM sensors had sensitivity in the range of 0.043–0.070 Hz/(mg/m 3 ), and their stability and reproducibility were above 93.1%. Four sensors that contributed to the classification were screened by a stepwise discriminant analysis. Afterward, Bayesian optimization was employed to optimize the hyperparameters. The accuracy of the optimized support vector machine (SVM) reached 94.17%. The olfactory detection system developed in this study enables the discrimination of citrus infested with B. dorsalis , which may have potential applications in the field of post-harvest treatment of citrus.
研究领域:
植物保护
·
食品科学与技术
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产品关联
DOI
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2023
·
IF 4.7
Zhenyu Li,Mengling Chen,Wenjie Bai,Shuxia Zhang,Liwei Meng,Wei Dou,Jinjun Wang,Guorui Yuan
Carboxylesterases (CarEs) are a multifunctional superfamily of enzymes and play an important role in detoxification of various insecticides in insects. The oriental fruit fly, Bactrocera dorsalis , is one of the most destructive agricultural pests and has developed different degrees of resistance to organophosphates in field. However, the involvement of BdCarEs in tolerance or resistance to other alternative insecticides are still unclear. In the present study, 33 BdCarEs genes were identified based on the genome database of B. dorsalis . Phylogenetic analysis demonstrated that they were classified into nine clades, with abundance of α -esterases. Meanwhile, the sequence characterization and the chromosome distribution were also analyzed. The spatiotemporal expression analysis of BdCarEs genes suggested that the diversity of potential function in different physiological processes . With the exception of BdCarE21 , all BdCarEs genes responded to at least one insecticide exposure, and BdCarE20 was found to be up-regulated after exposure to all five tested insecticides individually. Eight BdCarEs genes were overexpressed in MR strain when compared to that in SS strain. Subsequently, knockdown the expression of representative BdCarEs genes significantly increased the susceptibility of the oriental fruit fly to corresponding insecticides, which indicated that the tested BdCarEs genes contributed to one or multiple insecticide detoxification. These findings provide valuable insights into the potential role in respond to tolerance or resistance to insecticides with different mode of action, and will facilitate development of efficiency management strategy for B. dorsalis .
研究领域:
昆虫学
·
分子生物学
·
基因组学
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农药科学
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产品关联
DOI
PubMed
高影响因子
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