MOLECULES
·
2019
·Vol. 24
·期 14
·2621
·
IF 3.06
Weiwei Hu,Hui Gong,Lanqi Li,Shiguo Chen,Xingqian Ye
Blueberry pomace is abundant in anthocyanins. This work characterized the anthocyanins in blueberry pomace, discussed the stability of anthocyanins under ultrasound treatment, and compared the extraction conditions for different anthocyanin compositions. Thirteen anthocyanins were identified, and malvidin-3-galactoside (18.56%), which represented the most abundant anthocyanin, was selected as the individual analyte. The general linear model univariate analysis revealed that ultrasound-assisted extraction (UAE) resulted in higher recoveries of both total anthocyanins (TA) and individual anthocyanins (IA) when compared with conventional solvent extraction. The optimized extraction conditions for TA and IA were UAE in pure methanol (12.49 mg/g dry weight) at 25 °C for 30 min and UAE in 70% ethanol (3.57 mg/g dry weight) at 40 °C for 40 min, respectively. Moreover, IA was more vulnerable to degradation compared with TA. Therefore, a specific extraction process of IA is significant for monomer preparation, and harsh conditions should be avoided in UAE.Keywords:pomace;anthocyanin;identification;ultrasound-assisted extraction;conventional solvent extraction;comparison
研究领域:
食品科学
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化学
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植物化学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Shiwen Cheng,Yumei Qin,Yuezhong Mao,Yanyun Cao,Ruihang Zheng,Jianzhong Han,Shiyi Tian,Zihan Qin
Shelf life is a critical comprehensive indicator of food quality. Voltammetric electronic tongue (V-Et), is well-suited for assessing food shelf life, due to its capable of capturing food overall fingerprints. This study designed a “reference sample comparison method” for V-Et to assess the shelf life of fresh milk. Quality differences between milk samples of different shelf lives and reference samples were quantified by differential degree (D d ) values. A new “one-to-one” model of milk shelf life was established based on D d values, and significantly improved predictive accuracy by 11.14 %–17.17 % and 14.86 %–44.47 % in overall quality shelf life assessment compared to “many-to-one” models based on SVM and DFA. Even in the more sophisticated evaluation of microbial safety and sensory quality shelf life, it attained relative errors of 13.57 % and 7.68 %, respectively. All these findings showed the significant potential of the “reference sample comparison method” in assessing food shelf life with V-Et.
研究领域:
食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2022
·
IF 5.895
Wanjun Jin,Yu Lu,Cheng Li,Meiyi Zou,Qinghui Chen,Lijing Nan,Ming Wei,Chengjian Wang,Linjuan Huang,Zhongfu Wang
Sialylated human milk oligosaccharides (SHMOs) possess unique biological activities. Qualitative and quantitative analyses of SHMOs at different lactation stages are limited by interference from neutral oligosaccharides, glycan structural complexity, and low detection sensitivity. Herein, our previously developed glycoqueuing strategy was improved and applied to enable an isomer-specific quantitative comparison of SHMOs between colostrum milk (CM) and mature milk (MM). A total of 49 putative structures were determined, including 1 α2,6-linked and 13 α2,3-linked isomers separated from seven newly discovered SHMO compositions. The content of most oligosaccharides was more than 50% lower in MM than in CM, and α2,3-sialylation was observed in 43.74% of SHMOs from CM and 22.95% of SHMOs from MM. Finally, the fucosylation level of the SHMOs increased from 16.45 to 22.28% with prolonged lactation. These findings provide the basis for further studies on the structure–activity relationship of SHMOs and a blueprint to improve infant formula.
研究领域:
生物化学
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营养学
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儿科医学
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DOI
PubMed
高影响因子
BMC Chemistry
·
2023
·Vol. 17
·期 1
·1-10
·
IF 4.6
Xu Daokun,Huang Haolun,Hu Wenyan,Liu Xinmei,Yang Jun
A liquid chromatography–tandem mass spectrometry (LC–MS/MS) method was developed for the analysis of ribavirin in chicken. Samples was extracted with 0.1% formic acid and purified by Hypercarb cartridge prior to LC–MS/MS analysis. The eluates were evaporated to dryness, reconstituted in 1 mL 5mM ammonium acetate containing 5% acetonitrile (v/v) and 0.1% (v/v) formic acid. Chromatographic separation was performed on a Hypercarb analytical column under a gradient elution program with acetonitrile and 0.1% (v/v) formic acid in 5 mM ammonium acetate at a flow rate of 0.6 ml/min. The intraday and interday accuracy ranged from − 7.83 − 1.39%, and − 6.38 − 2.25%, with precisions between 1.34 − 3.88%% and 1.10 − 4.67%. The limits of detection (LODs) and limits of quantitation (LOQs) of ribavirin was 0.1 ng/mL and 0.5 ng/mL, respectively. The method was validated for linearity, accuracy, precision, matrix effect and stability. Application of the method confirmed 3 ribavirin positive samples out of 50 commercial chicken samples, with concentrations of ribavirin ranging from 0.9 μg/kg to 5.8 μg/kg a, respectively. Additionally, both AB Sciex 5500 and Agilent 6945B were proven to be suitable in ribavirin separation and quantification. The described method is suitable for the determination of ribavirin in chicken in analytical practice to monitor illegal addition of this kind of anti–viral drug.
研究领域:
分析化学
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兽药残留检测
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食品科学
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产品关联
DOI
PubMed
高影响因子
Microorganisms
·
2019
·Vol. 7
·期 10
·379
·
IF 4.167
Huanhuan Shao,Meiju Chen,Xueting Fei,Ronglin Zhang,Yue Zhong,Weimin Ni,Xiang Tao,XinYi He,Erliang Zhang,Bin Yong,Xuemei Tan
A bacterial strain,Streptomyces albogriseolusLBX-2, was isolated from a soil sample in Chengdu, China.S. albogriseolusLBX-2 is an aerobic and Gram-positive microorganism that is capable of using the polyethylene as the sole carbon source. Results of scanning electron microscopy and tensile tests indicated thatS. albogriseolusLBX-2 could cause the damages to polyethylene (PE). Suspension culture of LBX-2 resulted in the weight loss in the PE powder over a 15-day period. The bacterial growth curve assay clearly demonstrated the utilization of n-hexadecane and n-octadecane for the strain LBX-2. Phylogenetic analysis showed that it was grouped in the same clade asS. albogriseolusbelonging toStreptomyces. The complete genome of strain LBX-2 consists of a chromosome of 7,210,477 bp and a linear plasmid of 336,677 bp. Compared with other strains ofStreptomyces, the genome size ofS. albogriseolusLBX-2 was smaller than the average but its guanine and cytosine content (72.47%) was higher than the others. The Non-Redundant Protein Database (NR), Kyoto Encyclopedia of Genes and Genomes (KEGG), SwissProt, Gene Ontology (GO) and Clusters of Orthologous Groups (COG) annotations provided information on the specific functions of encoded proteins. A total of 21 monooxygenase and 22 dioxygenase genes were found in its genome. Synteny comparison with the genome ofStreptomyces coelicolorA3(2) revealed a low overall genetic diversity between them. This study provides valuable information to reveal the underlying mechanisms on PE degradation byS. albogriseolusLBX-2.Keywords:Streptomyces albogriseolusLBX-2;polyethylene biodegradation;genome;synteny comparison
研究领域:
微生物学
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环境科学
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基因组学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 19
·11144
·
IF 6.208
Qiuju Chen,Min Lian,Jian Guo,Binbin Zhang,Sankui Yang,Kexin Huang,Futian Peng,Yuansong Xiao
Nitrogen is an important nutrient element that limits plant growth and yield formation, but excessive nitrogen has negative effects on plants and the environment. It is important to reveal the molecular mechanism of high NUE (nitrogen use efficiency) for breeding peach rootstock and variety with high NUE. In this study, two peach rootstocks, Shannong–1 (S) and Maotao (M), with different NUE were used as materials and treated with 0.1 mM KNO3for transcriptome sequencing together with the control group. From the results of comparison between groups, we found that the two rootstocks had different responses to KNO3, and 2151 (KCL_S vs. KCL_M), 327 (KNO3_S vs. KCL_S), 2200 (KNO3_S vs. KNO3_M) and 146 (KNO3_M vs. KCL_M) differentially expressed genes (DEGs) were identified, respectively, which included multiple transcription factor families. These DEGs were enriched in many biological processes and signal transduction pathways, including nitrogen metabolism and plant hormone signal transduction. The function ofPpNRT2.1, which showed up-regulated expression under KNO3treatment, was verified by heterologous expression in Arabidopsis. The plant height, SPAD (soil and plant analyzer development) of leaf and primary root length of the transgenic plants were increased compared with those of WT, indicating the roles ofPpNRT2.1in nitrogen metabolism. The study uncovered for the first time the different molecular regulatory pathways involved in nitrogen metabolism between two peach rootstocks and provided gene reserve for studying the molecular mechanism of nitrogen metabolism and theoretical basis for screening peach rootstock or variety with high NUE.Keywords:peach;nitrogen use efficiency;comparative transcriptomics;PpNRT2.1gene;expression pattern;nitrate ion
研究领域:
植物科学
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分子生物学
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基因组学
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产品关联
DOI
PubMed
高影响因子
TOXICOLOGY IN VITRO
·
2021
·
IF 3.5
Shuyu Ouyang,Dachuan Zhang,Tengfei Zhu,Shuting Yu,Wunier,Qian-Nan Hu,Yingying Le
β 2 -adrenoceptor (β 2 AR) agonists can stimulate skeletal muscle growth. Their illegal use in food-producing animals, human athletes and bodybuilders causes adverse health effects. In the present study, we developed 3D-QSAR models for predicting the activity of chemicals which can stimulate skeletal muscle growth through β 2 AR. The activity of 25 β 2 AR agonist s was measured by β 2 AR-cAMP response element (CRE) –luciferase (Luc) reporter assay. The 3D-QSAR models were built using comparative molecular field analysis (CoMFA) and comparative molecular similarity indices analysis (CoMSIA). The CoMFA and CoMSIA models displayed high external predictability (R 2 0.996 and 0.992, respectively) and good statistical robustness, and revealed that electrostatic effects were the most prominent forces influencing the activity of β 2 AR agonists. The CoMFA and CoMSIA contour plots provided clues regarding the main chemical features responsible for the activity variations and also resulted in predictions which correlate very well with the observed activity. In vitro study with differentiated myotubes showed that the potency orders of β 2 AR agonists in activating the β 2 AR-CRE-Luc reporter and in upregulating CREB target genes related to muscle growth were consistent. These 3D-QSAR models provide tools for predicting the activity of chemicals which might be illegally used in livestock or humans to stimulate skeletal muscle growth.
研究领域:
药理学
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分子生物学
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生物信息学
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毒理学
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DOI
PubMed
高影响因子
3 Biotech
·
2021
·Vol. 11
·期 6
·1-13
·
IF 2.406
Huang Kai,Zhang Bo,Chen Yu,Wu Zhe-Ming,Liu Zhi-Qiang,Zheng Yu-Guo
Amphotericin, as an important macrolide antibiotic, is synthesized by Streptomyces nodosus . A high-yield S. nodosus ZJB2016050 was obtained by mutagenesis in our lab with the advantages of high yield, short fermentation cycle and few by-products, which was more suitable for industrial production. The fermentation differences in 50-tons bioreactor between S. nodosus ATCC14899 and S. nodosus ZJB2016050 were compared. The amphotericin B (AmB) yield of S. nodosus ZJB2016050 was 9.73 mg/g at 96 h, which was 30% higher than that of S. nodosus ATCC14899. The by-product amphotericin A (AmA) production of S. nodosus ZJB2016050 was 78% lower than that of S. nodosus ATCC14899. By performing whole-genome sequencing of S. nodosus ZJB2016050 and comparative genome analysis with the wild-type S. nodosus ATCC14899, it was found that the two strains have high synteny, but each has a special gene fragment. The genes functions of fragment were identified in the amino acid transport and metabolism, carbohydrate metabolism and lipid transport and metabolism. The gene functions of SNP (single nucleotide polymorphism) genes were identified in amino acid transport and metabolism, carbohydrate metabolism, coenzyme metabolism and secondary metabolites biosynthesis. The difference in signal-regulation and transcription may be the main reason for the differences between these two strains. Three GntR family egulatory factors of S. nodosus ATCC14899 may reduce the synthesis of amphotericin. Based on the analysis of comparative genomes, the effects of corn oil in S. nodosus ATCC14899 and S. nodosus ZJB2016050 were also compared. The results showed that corn oil can promote the fermentation of S. nodosus ZJB2016050. The S. nodosus ZJB2016050 may degrade fatty acids faster, and the degraded acyl-coenzyme can be used to synthesize amphotericin.
研究领域:
微生物学
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基因组学
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制药工程
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DOI
PubMed
高影响因子
ARABIAN JOURNAL FOR SCIENCE AND ENGINEERING
·
2017
·Vol. 43
·期 1
·423-432
·
IF 0.865
Yamani Zain Hassan
While most technologists use lamp light for the evaluation of catalysts synthesized for photocatalytic processes, some researchers prefer laser light. To conform in judgement, a comparative study of photocatalytically degrading methylene blue is reported, using a model catalyst Degussa P25, under different energies of 355 nm pulsed laser and under lamp (visible light and broader lamp light) irradiations. The used amounts of Degussa P25 after each batch of experiments were characterized by X-ray diffractometry, BET surface area analysis, field emission scanning electron microscopy, energy dispersive X-ray spectrometry and diffuse reflectance spectroscopy. The spectral photocatalytic degradation efficiencies, between broad lamp light and UV pulsed laser light, were comparable. Larger and spherical nanoparticles formation and reduction of BET surface area were noticed under pulsed UV laser beam irradiation of Degussa P25, while such trends were not observed under lamp irradiation for similar incident light intensities. The carbon contents on the surface, even after calcination of used samples at \(500\, ^{\circ }\) C, and the red shift in absorption spectra, were recorded in Degussa P25 used samples for both pulsed UV laser and lamp photocatalysis. Moreover, no change in the phases of Degussa P25 resulted under either types of irradiations during photocatalysis evaluation.
研究领域:
材料科学
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环境化学
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光催化
·
纳米材料
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表面科学
·
分析化学
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DOI
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2024
·
IF 6.4
Hua Huang,Xuemei He,Qingming Sun,Guoming Liu,Yayuan Tang,Jian Sun
Pitaya ( Hylocereus undatus ) fruit is susceptible to pathogens that induce fruit decay after harvest. Cuticular waxes covering the outermost plant surfaces are the first site of plant-environment interactions; however, their changes in response to fruit decay are under-researched. Herein, the surface microstructures and chemical composition of wax on pitayas, as well as their changes in response to fruit decay, were comparatively studied. The varieties ‘Baishuijing’ (BSJ) and ‘Hongshuijing’ (HSJ) were examined after harvest. The fruit appearance, morphology, and wax composition differed between cultivars. The surface of both pitaya cultivars was smooth, although traces of granules and a thicker lipid layer were observed using histochemical staining in HSJ than in BSJ, as well as a higher cuticular wax content. Fruit decay rate increased rapidly in both cultivars under room storage conditions, and greater sensitivity to pathogens was found in BSJ than HSJ. As well as the occurrence of fruit decay, the total wax coverage, very-long-chain primary alcohols, aldehydes, fatty acids, and triterpenoids decreased in BSJ, while remaining relatively stable in HSJ. Correspondingly, the relative gene expression levels of HuKCS11 (−1, 2, and 3) , HuKCS17 , and HuKCS19 (for the elongation of very-long-chain fatty acids), HuCER1 (for aldehydes or n -alkanes), HuFAR (−1 and 2) (for primary alcohols), HuBAS1 (−1, 3, and 4) (for β-amyrin), and HuLUS1 (for lupeol) were considerably modified following fruit senescence during storage. The difference in surface topography and wax accumulation between cultivars might have affected pathogen susceptibility after harvest. These results may help when choosing varieties of pitaya based on surface characteristics and cuticular chemicals. They may also help to optimize strategies for postharvest preservation and storage.
研究领域:
植物科学
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农学
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采后生理学
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分子生物学
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产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Guilan Jiang,Silin Li,Jingyi Zhu,Binbin Li,Zhuhong Ding
To investigate the conformational relationship and mechanism of action of β-glucosidase (BGL) activators, a theoretical study of three-dimensional quantitative structure activity relationship, molecular docking, molecular dynamics (MD) simulations, and binding free energy analysis were performed. The results indicate that comparative molecular field analysis (CoMFA) and comparative molecular similarity index analysis (CoMSIA) models had good stability and predictive ability, with r 2 cv / r 2 pred values of 0.540/0.998 and 0.654/0.993, respectively. The derived contour maps of steric, electrostatic, and hydrogen bond donor field further displayed the modified information of these activators. Molecular docking and MD were performed on calcium diascorbate (CaAS) and L-Ascorbyl 2,6-Dipalmitate (L-ADP), which have good activating ability. The study indicates a positive interaction between the activators and the enzyme, and this interaction does not cause irreversible negative effects on the conformation of BGL. The MD results show that the total binding free energies of CaAS and L-ADP with BGL were −19.837 kJ/mol and −37.798 kJ/mol, respectively, and the binding force of the activators with BGL was mainly derived from hydrophobic interaction and hydrogen bonding. This study offers important guidance for the efficient use of BGL and for the research and development of effective activators.
研究领域:
生物化学
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药物化学
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计算化学
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酶学
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2020
·
IF 4.006
Jiao Wu,Yun Duan,Zhaoyin Gao,Xinfeng Yang,Dongxiang Zhao,Jinglin Gao,Wensu Han,Guosheng Li,Shijie Wang
The physicochemical parameters, sugars, minerals and volatile compounds were selected in current work as the crucial parameters for evaluating multifloral honeys produced by Apis cerana cerana Fabricius, Apis dorsata Fabricius and Lepidotrigona flavibasis Cockerell. A summarized physicochemical profile of multifloral honeys exhibited great variability, which was quite different from that of A. mellifera honey, regarding color (83.86–328.36 mmPfund), ash (0.30–0.34 g 100 g −1 ), soluble solid (74.72–80.41 °Brix), diastase activity (5.48–6.70 °Göthe), electrical conductivity (0.40–0.55 mS cm −1 ), free acidity (0.51–0.85 mol kg −1 ), hydroxymethylfurfural (0–39.28 mg kg −1 ), moisture (18.65–23.80%) and pH (3.73–3.95). Fructose and glucose were the major carbohydrates in Apis spp. honeys but high level of maltose was present in L. flavibasis honey. The potassium , zinc and calcium were abundant elements in multifloral honeys. The principal component analysis was applied to discriminate honeys from different bee species by cobalt, sulfur, magnesium, color, maltose, pH, free acidity, potassium, molybdenum, F/G, HMF and iron, and it was concluded that L. flavibasis honey had better quality compared to the other honeys. Moreover, the specific volatile compounds detected in A. dorsata and L. flavibasis honeys were more than A. cerana cerana honey, which could be used as the characteristic markers discriminating honey samples.
研究领域:
农业
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食品科学
·
化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）