JOURNAL OF CONTROLLED RELEASE
·
2017
·
IF 7.786
Jingjing Hu,Yanchun Quan,Yuping Lai,Zhao Zheng,Zhiqi Hu,Xinyu Wang,Tianjiao Dai,Qiang Zhang,Yiyun Cheng
Aminoglycosides are a family of critically important antibiotics for the treatment of serious infections including multidrug-resistant pathogens. However, clinical use of aminoglycoside antibiotics is compromised by bacterial biofilm formation at subinhibitory concentrations or adverse side effects such as ototoxicity and nephrotoxicity at high antibiotic doses. Preparation of aminoglycoside formulation that allows on-demand drug delivery is a solution to this sticky issue. Here, we designed a new type of aminoglycoside hydrogels by cross-linking oxidized polysaccharides such as dextran , carboxymethyl cellulose , alginate , and chondroitin using aminoglycosides as cross-linkers. The hydrogel modulus, degradation rate and release kinetics can be precisely modulated by tailoring the aminoglycoside dose during gel formation. The aminoglycoside hydrogel showed fast gelation , self-healing and on-demand drug release behaviors, and high antibacterial activities in vitro and in vivo against both Gram-positive and Gram-negative bacteria. This study provides a facile and promising strategy to develop smart hydrogels for topical administration of aminoglycoside antibiotics.
研究领域:
药学
·
生物材料
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NANO
·
2013
·
IF 1.167
RUIYONG WANG,SHUMIN FAN,RUIQIANG WANG,RUI WANG,HUANJING DOU,LVJING WANG
A sensitive and selective colorimetric biosensor for determination of gentamicin, amikacin and tobramycin was proposed with the unmodified gold nanoparticles (GNPs) as the sensing element. Gentamicin, amikacin and tobramycin can rapidly induce the aggregation of gold nanoparticles and is accompanied by a color change from red to blue. The concentration of gentamicin, amikacin and tobramycin can be determined by using UV-Vis spectrometer. The experimental parameters were optimized with regard to pH, incubation time and the concentration of the GNPs. Under optimal experimental conditions, the linear range of the colorimetric sensor for gentamicin/amikacin/tobramycin were 2.67–33.93 ng mL -1 , 13.33–66.67 ng mL -1 and 20–180 ng mL -1 , respectively. The corresponding limit of detection (3σ) was 0.354 ng mL -1 , 0.999 ng mL -1 and 0.579 ng mL -1 , respectively. This assay was simple and used to detect aminoglycoside antibiotics in milk and medicine products.
研究领域:
分析化学
·
生物传感
·
药学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Guoxu Ao,Zhaoxuan Wang,Yueqi Shi,Hongzhi Ling,Shanshan Sun,Wenxiang Ping
Efficient elimination of antibiotics, particularly aminoglycosides, and Antibiotic resistance genes (ARGs) from agricultural and livestock waste, as well as enhancing the level of humification, remains a major challenge in the pursuit of high-value utilization of agricultural waste. Here, the roles of bacteria and fungus in the dissipation of aminoglycoside antibiotics, ARGs and the enhancement of humification with the addition of humic acid (HA) to compost systems were investigated. Compared to the control, the contents of humus (HS) and HA were increased by 41.3 and 143.3 %, respectively, and the content of fulvic acid (FA) was decreased by 74.1 % with HA addition. Interestingly, the abundance of aminoglycoside ARGs was decreased significantly ( p < 0.05) as well as the abundance of functional genes involved in oxidative stress response and type IV secretion system (T4SS). The bacterium Nocardiopsis related to carbon cycle, and the fungi Ascomycota and Basidiomycota associated with ligocellulose degradation were significantly enriched ( p < 0.05). Structural equation models revealed that the decrease in aminoglycoside ARGs abundance was attributed to bacteria promoting HA synthesis, thereby inhibiting the multiplication of aminoglycoside ARGs and ultimately mitigating oxidative damage to microbial cells. These findings shed light on the mechanism by which HA removes aminoglycoside ARGs, and provide valuable insights into enhancing the biosafety of compost products.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Xinglong Yang,Qin Wei,Huawu Shao,Xingyu Jiang
Bacterial infection, especially multidrug-resistant (MDR) bacteria-induced wound infection, is an enormous challenge and is the result of the inability of traditional antibiotics to combat MDR bacteria produced by the abuse of broad-spectrum drugs. Here, we present multivalent aminosaccharide-based gold nanoparticles (AuNPs) to remedy the superbug-infected wound. We synthesized multivalent aminosaccharide-based AuNPs via a straightforward method using d-glucosamine (GluN) to modify gold nanoparticles (AuNPs) as reported. This kind of multivalent aminosaccharide-based AuNP (Au_GluN) can lower the bacterial viability in a mature biofilm that may lead to antibiotic resistance. Au_GluN is innocuous not only for erythrocytes in vitro but also for mice. Moreover, it displays an outstanding ability for superbug-infected wound healing. Such a material provides new candidates to treat bacteria in the clinic.
研究领域:
生物医学工程
·
微生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2021
·
IF 4.759
Dan Wu,Peng Liu,Wenjia Fan,Ke Shi,Xi Luo,Feng Zheng,Shunli Ji
Bio-based and low-cost hybrid polyvinyl alcohol (PVA) and gelatin (Gel) hydrophilic macromolecular complex coated microspheres were prepared based on one-pot process, characterized, and applied as novel sorbent materials for the purification of trace aminoglycosides from complex matrices. PVA acts as a "rigid" component in the hybrid complex to enhance its mechanical properties, while Gel's "flexible" role is to improve the swelling properties of the hybrid complex in water. It is shown that hybrid PVA/Gel-functionalized sorbents are more efficient than the respective PVA or Gel sorbents since the presence of Gel increases the material selectivity for aminoglycosides, which is due to the specific interactions occurring between the targets and amino acid residues in the hybrid materials. Under the optimum conditions, material preparation and pretreatment processes were entirely carried out in single water system without toxic organic solvent. The detection limit (LOD) of spectinomycin, kanamycin, streptomycin and dihydrostreptomycin in honey were 0.811, 0.303, 0.168, 0.045 μg⋅kg −1 respectively. Linearity was obtained in the range of 20 to 2000 ug⋅kg −1 , relative recovery yield up to 84.1-111.7% were obtained and matrix effect of all four aminoglycosides was within 100.8-107.6%. Intra-day and inter-day precision under four spiking levels (5, 200, 500 and 1000 ug⋅kg −1 ) were less than 10.9% (n=6) and 13.6% (n=3) respectively. In addition, the sorbents exhibited excellent reusability even after six recycles. This work demonstrates the potential of bio-based and low-cost hybrid polymer extraction platforms as promising bonded phase alternatives, in which eco-friendly and natural-based polymers can be used to improve the material selectivity and are conducive to the realization of "green chemistry".
研究领域:
分析化学
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环境科学
·
生物材料
✓ 已验证
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2018
·Vol. 25
·期 17
·17128-17136
·
IF 2.8
Tan Lin-Rui,Xia Peng-Fei,Zeng Raymond J.,Li Qian,Sun Xue-Fei,Wang Shu-Guang
The interactions between antibiotics and microorganisms have attracted enormous research attentions. In this study, we investigated the effects of two typical aminoglycoside antibiotics on the aggregation of the model cyanobacterium, Synechococcus elongatus , and the dominating strain in algal blooms, Microcystis aeruginosa , via the analysis of zeta potentials, hydrophobicity, and extracellular polymeric substances (EPS) secretion . The results showed that low-level antibiotics promoted the aggregation of S. elongatus and M. aeruginosa by 40 and 18% under 0.10 and 0.02 μg/mL of kanamycin, respectively, which was mainly attributed to the combined effects of increased zeta potentials and the ratio between extracellular proteins and polysaccharides. Tobramycin exerted similar effects. Additionally, we discovered that at low pH (pH 5) and ionic strength (1 mM Na + and 2 mM Mg 2+ ), the inducing effects of antibiotics would be even larger than those with higher pH and ionic strength. As aggregation is important to cyanobacteria in either the basic physiology of biofilm formation or the algal bloom, our study demonstrated that low-level antibiotics exert ecological impacts via interfered aggregation. We believe this study will shed light on the mechanisms underlying antibiotic-induced biofilm formation and help with the evaluation of the environmental and ecological risks of antibiotics and other emerging pollutants.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Guang Yang,Changrong Wang,Yaping Wang,Xiaoqing Liu,Ye Zhang,Ming Xu,Hongzhang Deng,Weiwei Wang
Antibiotics still serve as the first choice for combating bacterial infections, whereas the therapeutic efficiency of antibiotics needs to be further augmented. Here, we report the spontaneous, generalized noncovalent co-assembly of aminoglycoside antibiotics (AGs) with tannic acid (TA) through electrostatic and hydrogen-bond interactions to form nanoparticles in water as off-the-shelf antibacterial agents. Specially, tobramycin@TA nanoparticles (Tob@TA NPs) elicited superior sterilization efficacy over native Tob against both Gram-positive and Gram-negative bacterial strains by enhancing the attachment onto the bacteria surface and the production of intracellular reactive oxygen species (ROS), disturbing membrane potential and promoting the leakage of bioactive substance such as DNA. Importantly, Tob@TA NPs also showed enhanced antibacterial efficacy against Tob-resistant Pseudomonas aeruginosa ( P. aeruginosa ). In P. aeruginosa -induced acute pneumonia model, the administration of Tob@TA NPs could eliminate bacterial infection and reduce inflammation. Furthermore, co-assembled Tob@TA NPs loaded in adhesive gelatin hydrogel dressing significantly accelerated the healing of P. aeruginosa -infected skin wound by effectively eradicating bacteria, decreasing inflammation and improving angiogenesis . Taking together, our findings provide a facile, simple and effective methodology to construct ready-to-use aminoglycoside antibiotics@TA NPs for the eradication of bacterial infection in clinic.
研究领域:
药学
·
感染病学
·
纳米医学
✓ 已验证
产品关联
DOI
高影响因子
BIOELECTROCHEMISTRY
·
2022
·
IF 5.76
Pinghua Ling,Shan Cheng,Linyu Wang,Xinyu Sun,Xianping Gao,Feng Gao
Herein, we reported the differential binding ability of aminoglycosides to DNA structures using electrochemical method through principal component analysis (PCA) to classify different DNA secondary structures and understand the link between secondary structure and DNA conformation. In these analyses, the DNA with different secondary structure motifs: bulge, internal loop, hairpin loop and stem loop were designed. The aminoglycosides as receptors were modified on the surface of electrode. In the presence of DNA, the DNA will be absorbed on the surface of electrode via the recognition of DNA and aminoglycosides, resulting in the electrochemical signal observed in [Fe(CN) 6 ] 3-/4- . Furthermore, the DNA structures labeled with 2-aminopurine (2-AP) at the structural motif of interest were also employed to study the binding affinity between aminoglycosides and different DNA motifs. The PCA suggested that this method may achieve nucleotide-specific classification of two independent secondary structure motifs, and the structure and sequence of DNA and the size and structure of small molecule could affect the binding ability of the aminoglycosides and DNA. This approach presents a new approach to classify DNA structure and offers ideas for designing targeted drugs small molecule compounds for wound dressing and drug delivery.
研究领域:
生物化学
·
分子生物学
·
药物设计
✓ 已验证
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 18
·4689-4696
·
IF 4.157
Li Xizhe,Li Bingyu,Liu Ruirui,Dong Yanhua,Wu Yayan
Sensitive and rapid identification of pathogenic microorganisms is of great importance for clinical diagnosis and treatment. In this study, we developed an ultrasensitive colorimetric sensor array (CSA) based on the interactions between aminoglycoside antibiotics (AMGs) and Ag nanoparticles decorated with β-cyclodextrin (AgNPs@β-CD) to discriminate microorganisms quickly and accurately. Microorganisms can absorb different amounts of AMGs after incubation. Upon the addition of AgNPs@β-CD, the corresponding extracellular AMG residues will bind to AgNPs@β-CD, leading to color changes due to the modifications in localized surface plasmon resonance. The array was developed using 4 AMGs as sensing elements and AgNPs@β-CD as the colorimetric probe to generate a unique colorimetric response pattern for each microorganism. Standard chemometric methods indicated excellent discrimination among 20 microorganisms at low concentrations of 2 × 10 6 CFU/mL. Therefore, this ultrasensitive CSA can be used for microbial discrimination portably and efficiently. Importantly, the concentration of microbial discrimination by our array is much lower than that of prior CSAs. This method of extracellular residue sensing also provided a new strategy to improve the sensitivity of conventional CSA in the discrimination of microorganisms, to measure the amount of intercellular uptake of AMGs by microorganisms, and to screen drugs that can easily be accumulated by the pathogenic microorganisms. Graphical abstract
研究领域:
临床诊断
·
生物化学
·
纳米技术
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Xuemei Li,Wenhua Ji,Rongyu Wang,Lidan Zhang,Ran Miao,Shenling Wang
Covalent organic framework molecularly imprinted polymers (CMIPs) was prepared using the thiol-ene click post-synthetic modification. The morphological, chemical characteristics and porosity were systematically characterized by scanning electron microscopy, Fourier-transform infrared spectroscopy, X-ray photoelectron spectroscopy, and N 2 adsorption–desorption. Isothermal adsorption, kinetic adsorption, and selective adsorption were used to evaluate the binding property of CMIPs. After being prepared as a solid-phase microextraction (SPME) fiber coating, the CMIPs-SPME pretreatment process for the extraction of six aminoglycosides was established after optimizing parameters such as desorption solvent and time, extraction time, pH value of the sample, and ion concentration. Under the optimized conditions, the CMIPs-SPME was combined with UPLC-MS/MS for the determination of trace aminoglycosides in milk and honey samples. The limits of quantification (S/N = 10) in milk and honey samples were 0.007–0.07 μg kg −1 and 0.04–0.1 μg kg −1 , respectively. The relative recoveries at three different spiked levels (1, 10, and 100 μg kg −1 ) for milk and honey are in the ranges of 86.2 %–115.3 % (RSD ≤ 9.6 %) and 88.1 %–114.3 % (RSD ≤ 10.4 %), respectively. The results indicated that the CMIPs-SPME pretreatment method for the determination of aminoglycosides in real samples is accurate, sensitive, and reliable.
研究领域:
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Maosai Zhang,Baojin Zhang,Tianbao Li,Xinhua Zhu,Wenchuan Guo
This work proposed a cost-effective, simple, and highly sensitive magnetic molecularly imprinted particles (MMIPs) electrochemical sensor to indirectly detect kanamycin (KAN), tobramycin (TOB), and gentamicin (GEN). The MMIPs electrochemical sensor was prepared by a complex of metal ions and the MMIPs of rebinding the template, which modified on the magnetic glassy carbon electrode surface. Here, metal ions were used as the signal tracers and amplifiers of the MMIPs electrochemical sensor. Then the response peak currents of metal re-oxidized to metal ion was recorded by differential pulse voltammetry and used to monitor the level of aminoglycoside antibiotics. Under the optimal conditions, the MMIPs electrochemical sensors showed a high sensitivity toward KAN, TOB, and GEN with detection limits of 4.88, 1.28, and 1.07 nmol/L, respectively. Furthermore, the MMIPs electrochemical sensors showed high selectivity for determining KAN, TOB, and GEN, and they were successfully used to detect KAN, TOB, and GEN in milk.
研究领域:
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Pharmacology
·
2025
·
IF 4.8
Lihua Yang,Hang Zhai,Tingting Tian,Botong Liu,Xianpu Ni,Huanzhang Xia*
Background: Aminoglycosides (AGs) are one of the initial classes of antibiotics that have been used clinically and possess broad spectrum of activity. Nevertheless, their clinical utilization is restricted by safety issues associated with nephrotoxicity and ototoxicity.Methods: Glycosyltransferase (GT) KanM2 was introduced into M. echinospora to produce the gentamicin derivatives, in which a kanosamine sugar ring was introduced to replace the garosamine. The premature termination codon (PTC) readthrough activity of genkamicins (GKs) was compared using dual luciferase reporter assay. The toxicity of GK was assessed in vitro in HEK-293 and NCI-H1299 cells and determined based on cell viability calculated after 48 h of treatment with different concentrations of the compounds. The NCI-H1299 cells harbouring the R213X nonsense mutation were treated with different concentrations of the derivatives to compare their expression of p53 proteins. The expression of p53 and its downstream targets p21 and BAX was detected using Western blotting and qRT-PCR in NCI-H1299 cells containing the R213X nonsense mutation treated with different concentrations of GK-Ae and G418. Finally, immunofluorescence and flow cytometry were used to determine the subcellular localization of full-length p53 protein induced by GK-Ae treatment and its effect on apoptosis in cancer cells.Results: Eight gentamicin derivatives were obtained in this study. GK-Ae displayed similar PTC readthrough activity and reduced toxicity compared to natural aminoglycoside G418. Moreover, GK-Ae increased the levels of both p53 and its downstream targets p21 and BAX, and promoted apoptosis of cancer cells.Conclusion: These results demonstrate the potential of combinatorial biosynthesis to increase the diversity of structures of AGs and provide directions for the development of new AGs with low toxicity and high PTC readthrough activity.
研究领域:
药理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）